LOCUS       RR217598                8943 bp    DNA     circular  SYN 
DEFINITION  Mink1 (myc-DDK-tagged) - Rat misshapen-like kinase 1 (Mink1)
ACCESSION   NM_001271136
VERSION     NM_001271136.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5100..5191 
                     /label="Myc-DDK"  
     misc_feature    5221..5806 
                     /label="PolyA signal"  
     misc_feature    5278..5297 
                     /label="Restriction Enzyme"  
     misc_feature    6082..6754 
                     /label="ColE1"  
     misc_feature    7184..7978 
                     /label="Kan/Neo"  
     misc_feature    8013..8315 
                     /label="SV40 ori"  
     misc_feature    8401..8878 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5082..5088 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5082 
                     /label="Mink1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACCCA GCCCCCGCCC GAAGCTTGGA CGACATCGAC CTGTCTGCCC 
     1081 TGAGGGACCC TGCAGGAATC TTTGAGCTGG TGGAGGTGGT TGGCAATGGA ACCTATGGAC 
     1141 AGGTGTACAA GGGGCGGCAC GTCAAGACTG GGCAGCTGGC TGCCATTAAG GTCATGGATG 
     1201 TCACAGAGGA TGAGGAGGAA GAAATCAAAC AGGAAATCAA CATGTTAAAG AAGTACTCTC 
     1261 ACCATCGCAA TATTGCCACC TACTACGGGG CCTTCATCAA GAAGAGCCCT CCTGGGAACG 
     1321 ATGACCAGCT CTGGCTGGTG ATGGAGTTCT GTGGTGCTGG CTCAGTGACC GACCTGGTAA 
     1381 AGAACACGAA AGGAAACGCG CTGAAAGAGG ATTGCATTGC GTACATCTGC AGGGAGATTC 
     1441 TCAGGGGTCT TGCCCATCTC CATGCCCACA AGGTGATCCA CCGAGATATC AAGGGACAAA 
     1501 ATGTGCTGCT GACAGAGAAT GCTGAAGTCA AGCTAGTGGA CTTTGGGGTG AGTGCTCAGC 
     1561 TGGACCGCAC TGTGGGGAGG CGGAACACTT TCATTGGGAC CCCATACTGG ATGGCTCCAG 
     1621 AAGTCATTGC CTGTGACGAG AACCCCGATG CCACCTATGA CTACAGGAGT GACATTTGGT 
     1681 CTCTAGGAAT CACAGCCATT GAAATGGCAG AGGGAGCCCC CCCTCTGTGT GACATGCACC 
     1741 CTATGCGGGC TCTCTTCCTC ATCCCTCGGA ACCCTCCCCC CAGGCTCAAG TCAAAGAAAT 
     1801 GGTCTAAGAA GTTCACTGAC TTCATTGACA CGTGTCTCAT CAAGACTTAC CTGAGCCGAC 
     1861 CACCAACAGA ACAGTTACTC AAATTCCCCT TCATCCGAGA CCAGCCCACG GAGCGGCAGG 
     1921 TCCGCATCCA GCTCAAGGAC CACATTGACC GCTCCCGGAA GAAGCGGGGG GAGAAAGAGG 
     1981 AGACAGAGTA TGAGTACAGT GGCAGTGAAG AGGAAGACGA CAGCCATGGA GAGGAAGGCG 
     2041 AGCCAAGTTC CATCATGAAT GTGCCTGGGG AGTCCACACT CCGCCGAGAA TTCCTCAGAC 
     2101 TCCAACAGGA GAATAAGAGC AACTCAGAGG CTTTAAAGCA GCAGCAGCAG CTGCAGCAGC 
     2161 AGCAGCAGCG GGACCCTGAG GCACACATCA AACACCTGCT GCACCAGCGG CAGCGTCGCA 
     2221 TAGAGGAGCA GAAGGAGGAG CGGCGACGTG TAGAGGAGCA ACAGCGGCGA GAGCGAGAAC 
     2281 AGCGCAAGCT GCAGGAGAAG GAGCAGCAGC GGCGGTTGGA AGACATGCAA GCCCTTCGTC 
     2341 GAGAGGAAGA GAGGCGGCAA GCAGAGCGGG AACAGGAATA CAAGCGGAAG CAGCTGGAGG 
     2401 AGCAGCGGCA GTCGGAGCGG CTCCAGAGAC AGCTGCAGCA GGAGCATGCC TACCTCAAGT 
     2461 CCCTGCAGCA GCAGCAGCAA CAGCAGCAGC AGCTCCAGAA ACAACAGCAG CAGCAGCAGC 
     2521 AGCAGCAGCA GCAGCAGCAG CAGCAGCAGA TCCTACCTGG AGACAGGAAA CCCCTGTATC 
     2581 ATTATGGTCG GGGCATTAAT CCTGCTGACA AGCCAGCATG GGCCCGAGAG GTGGAAGAGA 
     2641 GAGCTCGGAT GAACAAGCAG CAGAACTCTC CCTTGGCCAA GACAAAGCCA AGCAGTGCGG 
     2701 GGCCAGAGCC CCCCATTCCC CAGGCCTCTC CTAGCCCCCC AGGACCTCTT TCCCAAACTC 
     2761 CTCCTATGCA GAGGCCTGTG GAGCCTCAGG AAGGACCACA CAAGAGCCTG GTGGCACACC 
     2821 GGGTCCCACT GAAGCCATAT GCAGCACCTG TACCCCGATC CCAGTCCCTG CAGGACCAAC 
     2881 CCACTCGAAA CTTGGCTGCC TTCCCAGCCT CCCACGACCC TGACCCTGCT GCTGTCCCCA 
     2941 CACCCACTGC CACACCCAGT GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT 
     3001 CTGAAGGGCC TGGGCCTAGC CCTAACCCTC CATCCTGGGT CCGGCCTGAT AATGAAGCTC 
     3061 CACCCAAGGT TCCTCAGAGG ACCTCGTCTA TTGCCACTGC CCTTAACACC AGTGGGGCTG 
     3121 GAGGGTCCCG GCCAGCTCAG GCTGTCCGTG CCAGACCTCG CAGCAACTCC GCCTGGCAAA 
     3181 TCTATCTGCA GAGGCGGGCA GAGCGAGGCA CCCCCAAGCC TCCTGGGCCC CCAGCTCAGC 
     3241 CCCCTGGCCC GCCCAACACC TCTAGTAACC CTGACCTCAG GAGGAGTGAC CCTGGCTGGG 
     3301 AGCGTTCAGA CAGTGTCCTC CCTGCCTCCC ACGGCCACCT CCCTCAGGCC GGCTCCTTGG 
     3361 AGCGGAACCG AAACCGTGTG GGAGCCTCCA CAAAACTGGA CAGCTCCCCA GTGCTCTCCC 
     3421 CTGGGAACAA GGCCAAGCCT GAAGACCACC GCTCAAGGCC AGGCCGGCCC GCAAGCTATA 
     3481 AGCGAGCGAT TGGTGAGGAT TTCGTGTTGC TCAAAGAGCG GACTCTGGAT GAGGCCCCTA 
     3541 AGCCTCCCAA GAAGGCCATG GACTATTCCT CATCCAGTGA GGAGGTGGAG AGCAGTGAAG 
     3601 ATGAGGAGGA GGAAGGCGAT GGGGAGCCAT CAGAGGGGAG CAGAGACACT CCCGGGGGCC 
     3661 GTGATGGTGA CACAGATAGC GTCAGCACCA TGGTGGTTCA TGATGTTGAA GAGGTATCCG 
     3721 GGACCCAGCC TTCATATGGA GGAGGCACCA TGGTGGTCCA GCGTACTCCT GAAGAGGAGC 
     3781 GAAGCCTGCT GCTTGCTGAT AGCAATGGCT ACACAAACCT GCCAGATGTG GTCCAGCCCA 
     3841 GCCACTCACC TACTGAGAAC AGCCAAGGTC AAAGCCCTCC AACAAAGGAT GGAGGCGGTG 
     3901 ATTACCAGTC TCGTGGGCTG GTAAAGGCCC CAGGAAAGAG TTCATTCACC ATGTTTGTGG 
     3961 ATCTAGGGAT CTACCAGCCT GGAGGCAGTG GGGATACCAT CCCCATCACA GCCCTAGTGG 
     4021 GCGGAGAGGG TGGTCGCCTT GATCAACTGC AGTTCGATGT GAGGAAGGGC TCTGTGGTCA 
     4081 ACGTCAATCC CACCAACACT CGAGCTCATA GTGAAACTCC CGAGATTCGC AAGTACAAGA 
     4141 AGCGATTCAA TTCCGAGATC CTCTGTGCAG CCCTCTGGGG GGTCAACCTC CTAGTGGGCA 
     4201 CAGAGAATGG GCTGATGTTG CTGGACCGAA GTGGGCAGGG CAAAGTGTAT GGACTTATTG 
     4261 GGCGACGACG CTTCCAGCAA ATGGATGTTC TGGAAGGGCT CAACCTGCTC ATCACCATCT 
     4321 CAGGGAAAAG GAACAAACTG CGGGTATATT ACCTGTCCTG GCTTCGGAAT AAGATTCTAC 
     4381 ACAATGACCC AGAGGTGGAA AAGAAGCAGG GGTGGACCAC CGTGGGGGAC ATGGAGGGCT 
     4441 GCGGCCACTA TCGTGTTGTG AAATATGAAC GAATTAAGTT CCTGGTCATT GCTCTGAAGA 
     4501 ACTCTGTGGA GGTGTATGCC TGGGCCCCTA AACCCTACCA CAAATTCATG GCCTTCAAGT 
     4561 CCTTTGCTGA CCTCCCTCAC CGCCCTCTGC TGGTGGACCT GACAGTAGAG GAGGGCCAGC 
     4621 GGCTCAAGGT CATCTATGGC TCCAGTGCTG GCTTCCATGC TGTGGATGTT GACTCTGGGA 
     4681 ACAGCTATGA CATCTACATC CCTGTACATA TCCAGAGCCA AATCACACCC CACGCCATCG 
     4741 TCTTTCTCCC CAACACTGAC GGCATGGAGA TGCTGCTTTG CTATGAAGAT GAGGGTGTCT 
     4801 ACGTCAACAC TTACGGGCGG ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA 
     4861 CCTCTGTCGC CTACATCTGC TCCAACCAGA TAATGGGCTG GGGTGAGAAG GCCATAGAGA 
     4921 TCCGCTCTGT GGAGACAGGC CACCTAGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC 
     4981 TCAAGTTCCT GTGTGAGCGG AATGATAAGG TGTTTTTTGC CTCAGTCCGC TCTGGAGGAA 
     5041 GCAGCCAAGT TTACTTTATG ACTCTGAACC GTAACTGCAT CATGAACTGG ACGCGTACGC 
     5101 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5161 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5221 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5281 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5341 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5401 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5461 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5521 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5581 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5641 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5701 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5761 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5821 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5881 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5941 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6001 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6061 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6121 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6181 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6241 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6301 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6361 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6421 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6481 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6541 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6601 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6661 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6721 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6781 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6841 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6901 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6961 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7021 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7081 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7141 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7201 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7261 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7321 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7381 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7441 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7501 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7561 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7621 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7681 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7741 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7801 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7861 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7921 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7981 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8041 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8101 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8161 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8221 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8281 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8341 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8401 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8461 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8521 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8581 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8641 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8701 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8761 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8821 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8881 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8941 TTT
//