LOCUS       RR217596                8919 bp    DNA     circular  SYN 
DEFINITION  Whsc1 (myc-DDK-tagged) - Rat Wolf-Hirschhorn syndrome candidate 1 (Whsc1)
ACCESSION   NM_001191552
VERSION     NM_001191552.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5076..5167 
                     /label="Myc-DDK"  
     misc_feature    5197..5782 
                     /label="PolyA signal"  
     misc_feature    5254..5273 
                     /label="Restriction Enzyme"  
     misc_feature    6058..6730 
                     /label="ColE1"  
     misc_feature    7160..7954 
                     /label="Kan/Neo"  
     misc_feature    7989..8291 
                     /label="SV40 ori"  
     misc_feature    8377..8854 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5058..5064 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5058 
                     /label="Nsd2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCAGGCA CCAGAAATCC TTGGCAGTGC AAATGGAAAG ACTCAGAACT 
     1081 GTGAAGTGAA TCACGAATGT TCTGTATTCC TCAGCAAAGC TCAGCTTTCT AACAGCCTAC 
     1141 AGGAGGGGGT CATGCAGAAA TTTAACGGGC ATGATGCACT CCCCTTTCTT CCAGCAGAGA 
     1201 AGTTGAAAGA TCTTACTTCT TGTGTGTTTA ATGGAGAACC TGGTGCTCAT GATACTAAGT 
     1261 TGTGTTTTGA GACCCAGGAA GTAAAAGGAA TTGGGACACC ACCCAATACT ACTCCTATCA 
     1321 AAAATGGCTC TCCAGAAATT AAACTGAAAA TCACCAAAAC ATACATGAAT GGGAAACCTC 
     1381 TCTTTGAATC TTCAATTTGT GGTGATGGCG CTGCTGATAT GTCTCAGTCA GAAGAAAATG 
     1441 GACAAAAATC GGATAATAAG ACTAGGAGGA ACAGGAAGAG GAGCATAAAA TATGACTCTT 
     1501 TGCTGGAGCA GGGCCTTGTG GAAGCAGCTT TAGTGTCCAA GATCTCAAGT CCTGAAGATA 
     1561 AAAAGATTCC AGTTAAGAAG GAGTCCTGTC CAAATAGTGG CAGAGACAGA GACCTCTTGT 
     1621 TAAAATACAA CGTTGGTGAT TTGGTGTGGT CCAAAGTGTC AGGTTACCCT TGGTGGCCTT 
     1681 GCATGGTTTC TGCTGATCCA CTCCTTCACA ATCATACCAA ACTTAAAGGT CAGAAAAAAA 
     1741 GTGCACGCCA GTATCATGTA CAATTCTTTG GTGATGCCCC AGAAAGAGCT TGGATATTTG 
     1801 AGAAGAGCCT TGTTGCTTTT GAAGGAGAAG AACAATTTGA AAAATTATGC CAAGAAAGTG 
     1861 CCAAGCAGGC ACCCACGAAA GCTGAGAAAA TCAAGTTGTT GAAACCTATT TCAGGGAGAT 
     1921 TGAGAGCCCA GTGGGAAATG GGCATTGTCC AGGCTGAGGA AGCTGCTAGC ATGTCAGTAG 
     1981 AGGAGCGGAA AGCCAAGTTT ACTTTCCTTT ATGTGGGGGA CCAACTGCGC CTCAACCCTC 
     2041 AAGTGGCTAA GGAGGCTGGC ATTGCTACAG AACCTTTGGG AGAAATGGTG GACTCTTCAG 
     2101 TGGCCAATGA AGAAGCTGCT GTAGACCCTG GGACTATGAG AGAAGAGGAC ATTCCCGTAA 
     2161 AGAGAAGGCG AAGAGCCAAA AGGTCTAGTT CTGCTGAGAA CCAGGAAGGA GACCCCGGCA 
     2221 CAGAAAAGAG TACACCTCCA AAGATGGCGG ATGCTGAACC TAAGAGAGGC GTAGGTTCTC 
     2281 CTGCCGGGAG GAAAAGGTCC ACAGGATCTG CCTCACGGAG CAGAAAGGGA GACTCAGCAG 
     2341 CCCAGTTCTT AGTCTTTTGT CAAAAACACA GGGATGAGGT CGTAGCTGAA CACCCAGATG 
     2401 CCTCAGAGGA GGAGATTGAA GAATTGCTTG GGTCCCAATG GAGCATGCTC AATGAAAAGC 
     2461 AGAAAGCACG ATATAATACA AAGTTTTCCC TAATGATCTC TGCCCAGTCT GAAGAAGACT 
     2521 CTGGAAATAC CAGTGGGAAA AAAAGGACCC ACACAAAAAG GACAGATGAC CCTCCTGAGG 
     2581 ATGTGGATGT AGAAGACGCA CCCAGGAAAA GACTTAGGAC AGATAAGCAC AGTCTTCGGA 
     2641 AGCAAAGAGA GACAATCACC GACAAAACGG CCAGAACGAG CTCGTACAAG GCTATAGAGG 
     2701 CAGCCTCCTC ACTCAAGAGC CAGGCAGCAA CGAAAAATTT GTCTGATGCT TGCAAACCAC 
     2761 TGAAGAAGCG AAATCGGGCT TCTGCAACAG CGTCTTCAGC ACTTGGGTTT AACAAAAGTT 
     2821 CATCTCCTTC TGCATCCTTA ACTGAGAATG AGGTCTCAGA CAACCCAGGA GATGAACCGT 
     2881 CAGAGTCTCC GTATGAAAGT GCAGATGAAA CACAGACTGA AGCGTCCGTC TCATCTAAGA 
     2941 AATCTGAAAG AGGAATGGCT GCCAAAAAGG AGTACGTGTG TCAGCTGTGT GAGAAGACAG 
     3001 GCAGTCTCTT ACTGTGTGAA GGCCCCTGTT GTGGAGCATT CCACTTAGCC TGCCTTGGAC 
     3061 TTTCCCAGAG ACCAGAAGGA AGATTCACCT GCACTGAATG TGCCTCAGGG ATTCACTCAT 
     3121 GCTTCGTATG TAAAGAAAGC AAGATGGAGG TGAAACGCTG TATGGTAAAC CAATGTGGAA 
     3181 AGTTTTACCA TGAAGCCTGT GTGAAAAAAT ACCCTCTCAC TGTGTTTGAG AGCCGAGGCT 
     3241 TTCGCTGTCC TCTCCACAGC TGTATGAGCT GCCATGCCTC AAACCCTTCA AATCCGCGGC 
     3301 CATCAAAAGG TAAAATGATG CGGTGCGTCC GATGCCCTGT CGCCTATCAT GGCGGGGATG 
     3361 CCTGCCTGGC AGCGGGATGT TCCGTGATTG CTTCTAACAG CATAATCTGC ACAGGCCACT 
     3421 TCACTGCCCG CAAGGGGAAG CGGCACCACA CTCATGTCAA TGTGAGCTGG TGCTTCGTGT 
     3481 GCTCCAAAGG GGGAAGCCTT CTGTGCTGCG AGGCCTGTCC GGCAGCCTTC CACCCTGATT 
     3541 GTTTGAGCAT CGAGATGCCT GATGGCAGCT GGTTCTGCAA TGACTGCCGA GCTGGGAAGA 
     3601 AGCTCCACTT TCAAGACATC ATTTGGGTCA AACTTGGGAA CTACAGATGG TGGCCAGCAG 
     3661 AAGTTTGCCA TCCCAAAAAT GTTCCCCCAA ATATTCAGAA AATGAAGCAC GAGATTGGAG 
     3721 AATTCCCTGT GTTTTTCTTC GGGTCTAAAG ATTATTACTG GACGCATCAG GCACGAGTGT 
     3781 TCCCATACAT GGAGGGGGAC CGGGGCAGCC GCTACCAGGG GGTCAGAGGG ATTGGAAGAG 
     3841 TCTTCAAGAA CGCATTGCAA GAAGCCGAAG CTCGTTTTAA TGAAATCAAA CTTCAGCGGG 
     3901 AAGCCCGAGA AACACAGGAG AGTGAGAGAA AGCCTCCACC ATACAAGCAT ATCAAGGTGA 
     3961 ATAAACCTTA TGGTAAAGTC CAGATCTATA CAGCTGACAT TTCTGAAATC CCAAAATGCA 
     4021 ACTGTAAGCC CACGGATGAG AACCCCTGTG GCTCTGATTC AGAGTGTCTA AACAGGATGC 
     4081 TAATGTTTGA GTGCCACCCG CAGGTGTGCC CTGCAGGGGA ATATTGCCAG AACCAGTGTT 
     4141 TCACTAAGCG CCAGTACCCG GAGACCAAGA TCATCAAGAC CGATGGCAAA GGGTGGGGCC 
     4201 TGGTTGCCAA AAGGGACATC AGAAAGGGAG AATTTGTTAA TGAGTATGTT GGCGAACTGA 
     4261 TTGATGAAGA GGAGTGTATG GCAAGAATCA AATATGCACA TGAGAATGAC ATCACTCACT 
     4321 TTTACATGCT CACCATAGAC AAGGACCGCA TAATTGATGC TGGCCCCAAA GGGAATTACT 
     4381 CGCGGTTCAT GAACCATAGC TGCCAGCCCA ACTGTGAGAC CCTGAAGTGG ACAGTGAATG 
     4441 GGGACACACG GGTTGGCCTG TTTGCTGTGT GCGACATCCC TGCAGGTACA GAGCTGACTT 
     4501 TCAACTATAA CCTCGATTGT TTGGGCAATG AAAAGACAGT CTGTCGGTGT GGAGCCTCCA 
     4561 ACTGTAGTGG GTTCCTTGGA GACAGACCAA AGACATCAAC ATCCCTTTCT TCAGAGGAAA 
     4621 AGAGTAAAAA GGCCAAGAAG AAAACCAGGA GGCGCAGAGC CAAGGGTGAA GGAAAGCGGC 
     4681 AGTCAGAGGA CGAATGCTTC CGCTGTGGTG ATGGCGGACA ACTGGTGCTG TGTGACCGCA 
     4741 AGTTCTGCAC CAAGGCCTAC CACCTATCCT GCTTGGGCTT GGGCAAGCGG CCCTTTGGGA 
     4801 AGTGGGAATG TCCTTGGCAT CACTGTGATG TGTGTGGCAA ACCTTCTACC TCATTTTGCC 
     4861 ACCTCTGCCC CAACTCATTC TGTAAGGAAC ACCAAGATGG GACTGCTTTC CGCTCCACCC 
     4921 AGGATGGGCA ATCCTACTGC TGTGAGCATG ACCTGAGGGC AGACTCTGCA AATAATACCA 
     4981 AGACTGAGAA ACCCTTTCTA GACTCACTCA AGGCGAAGGG GAAGAGGAAG AAAAGGCGGT 
     5041 GCTGGCGAAG GGTCACAGAC GGCAAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5101 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5161 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5221 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5281 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5341 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5401 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5461 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5521 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5581 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5641 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5701 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5761 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5821 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5881 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5941 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6001 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6061 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6121 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6181 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6241 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6301 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6361 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6421 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6481 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6541 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6601 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6661 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6721 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6781 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6841 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6901 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6961 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7021 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7081 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7141 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7201 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7261 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7321 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7381 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7441 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7501 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7561 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7621 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7681 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7741 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7801 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7861 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7921 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7981 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8041 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8101 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8161 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8221 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8281 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8341 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8401 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8461 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8521 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8581 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8641 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8701 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8761 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8821 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8881 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//