LOCUS       RR217591                8862 bp    DNA     circular  SYN 
DEFINITION  Irs2 (myc-DDK-tagged) - Rat insulin receptor substrate 2 (Irs2)
ACCESSION   NM_001168633
VERSION     NM_001168633.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5019..5110 
                     /label="Myc-DDK"  
     misc_feature    5140..5725 
                     /label="PolyA signal"  
     misc_feature    5197..5216 
                     /label="Restriction Enzyme"  
     misc_feature    6001..6673 
                     /label="ColE1"  
     misc_feature    7103..7897 
                     /label="Kan/Neo"  
     misc_feature    7932..8234 
                     /label="SV40 ori"  
     misc_feature    8320..8797 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5001..5007 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5001 
                     /label="Irs2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTAGCGCG CCCCTGCCTG GGCCCCCCGC GTCGGCGGGC GGGGACGGCC 
     1081 CGAACCTCAA TAACAACAAC AACAACAACA ACAACCACAG CGTGCGCAAG TGCGGCTACC 
     1141 TGCGCAAGCA GAAGCACGGC CACAAGCGCT TCTTCGTGCT GCGCGGCCCG GGCACCGGCG 
     1201 GCGAGGAGGC AGCCGCGGCT GGGGGGTCGC CGCCGCAGCC TCCGCGGCTG GAGTACTATG 
     1261 AGAGCGAGAA GAAGTGGAAG AGCAAGGCGG GCGCGCCGAA GCGAGTGATC GCGCTCGACT 
     1321 GCTGTCTGAA CATCAACAAG CGCGCGGACG CCAAGCACAA GTACCTGATC GCCCTCTACA 
     1381 CCAAGGACGA GTACTTCGCC GTAGCGGCGG AGAACGAGCA GGAGCAGGAG GGCTGGTACC 
     1441 GCGCACTCAC CGACTTGGTC AGCGAAGGCC GCTCTGGCGA TGGGGGCTCC GGCACCACGG 
     1501 GCGGCTCTTG CAGCGCCTCT CTCCCGGGGG CCCTGGGCGG CTCGGCGGGC GCCGCTGGCT 
     1561 GCGATGACAA CTACGGGCTC GTGACGCCCG CCACGGCAGT CTACCGCGAG GTGTGGCAGG 
     1621 TGAACCTGAA GCCTAAGGGA CTGGGCCAGA GCAAGAACCT GACCGGTGTA TACCGCCTAT 
     1681 GCCTGTCTGC GCGCACCATC GGCTTCGTGA AGCTCAATTG CGAACAGCCG TCGGTGACGC 
     1741 TGCAGCTTAT GAACATTCGC CGCTGCGGCC ACTCGGACAG CTTCTTCTTC ATCGAGGTGG 
     1801 GCCGTTCAGC CGTCACCGGT CCCGGCGAGC TGTGGATGCA AGCCGATGAC TCGGTGGTGG 
     1861 CGCAGAACAT CCATGAGACC ATCCTGGAGG CCATGAAGGC ACTCAAGGAG CTCTTCGAGT 
     1921 TCCGGCCTCG CAGCAAGAGT CAGTCGTCTG GGTCGTCTGC CACGCACCCC ATCAGCGTAC 
     1981 CGGGCGCGCG CCGCCACCAC CACCTAGTCA ACCTACCCCC TAGCCAGACG GGCCTGGTGC 
     2041 GCCGCTCTCG CACTGATAGC CTGGCGGCCA CCCCGCCAGC AGCCAAGTGC ACTTCGTGCC 
     2101 GGGTTCGTAC AGCCAGCGAG GGTGACGGCG GCGCGGCAGG CGGAGCCGGG ACGGCAGGAG 
     2161 GCAGGCCGAT GTCGGTGGCA GGGAGCCCCC TGAGTCCCGG GCCGGTGCGC GCGCCCCTTA 
     2221 GCCGCTCGCA CACCCTGAGC GCCGGCTGCG GAGGCCGCCC GAGCAAAGTG GCTCTGGCGC 
     2281 CGGCAGGGGG AGCCCTACAA CACAGCCGCT CCATGTCCAT GCCCGTGGCG CACTCGCCGC 
     2341 CTGCAGCCAC CAGCCCAGGC AGCCTGTCCT CCAGCAGTGG GCATGGCTCA GGCTCCTACC 
     2401 CGCTGCCTCC TGGTTCCCAC CCGCACCTGC CTCATCCGCT CCATCACCCC CAATGCCAAC 
     2461 GTCCGTCCAG CGGAAGTGCC TCCGCCTCGG GCTCCCCCAG CGACCCGGGT TTCATGTCCC 
     2521 TTGACGAGTA TGGCTCCAGC CCTGGTGACC TGAGAGCCTT CAGTAGCCAC AGGAGCAACA 
     2581 CACCTGAGTC CATCGCGGAG ACCCCGCCAG CCAGGGACGG CAGTGGGGGC GAGCTCTATG 
     2641 GGTATATGAG CATGGATAGG CCCCTGAGCC ACTGTGGCCG CCCTTACCGT AGGGTCTCTG 
     2701 GGGATGGGGC CCAGGATCTG GACAGAGGAC TGAGGAAGAG GACTTACTCC CTAACCACGC 
     2761 CTGCCCGGCA GCGGCAGGTT CCTCAGCCTT CCTCTGCCTC TCTAGACGAA TACACTCTCA 
     2821 TGCGGGCCAC CTTCTCTGGC AGTTCAGGTC GCCTCTGCCC ATCCCTCCCT GCGTCCTCTC 
     2881 CCAAAGTGGC CTACAACCCT TACCCAGAGG ACTATGGAGA CATTGAGATT GGTTCTCACA 
     2941 AGAGTTCCAG CAGTAACCTG GGGGCAGATG ATGGCTACAT GCCCATGACC CCTGGGGCAG 
     3001 CCCTCAGGAG TGGTGGCCCC AATAGCTGCA AGAGCGATGA CTACATGCCC ATGAGCCCCA 
     3061 CCAGCGTGTC TGCCCCTAAG CAGATCCTGC AACCACGTTC GGCAGCGGCC TTGCCCCCCT 
     3121 CTGGAGCAGC CGTGCCAGCA CCCCCTTCAG GGGCGGGCAG GACTTTCCCA GTGAACGGAG 
     3181 GCGGCTACAA AGCCAGCTCC CCAGCGGAGA GCTCCCCAGA AGATAGCGGG TACATGCGAA 
     3241 TGTGGTGTGG CTCCAAGCTG TCCATGGAGA ACCCAGACCC TAAGCTGCTC CCCAATGGGG 
     3301 ACTACCTCAA CATGTCCCCC AGTGAGGCAG GCACCGCAGG GACCCCACCT GACTTCTTCT 
     3361 CAGCAGCTTT GCGTCCAGGC GGTGAGGCCC TCAAAGGCGT CCCTGGCCAC TGCTACAGCT 
     3421 CTTTGCCCCG CTCTTACAAG GCTCCCTGTA CTTGCGGTGG TGGAGACAAC GACCAGTACG 
     3481 TGCTCATGAG CTCCCCTGTG GGTCGGATTT TGGAAGAGGA GAGACTGGAG CCCCAGGCCA 
     3541 CCCCAGGGGC TGGCACATTT GGGGCAGCTG GTGGTAGTCA TACCCAGCCT CATCACTCAG 
     3601 CAGTGCCTTC CTCCATGAGA CCAAGTGGCA TCGTTGGCCG ACCTGAGGGC TTCCTGGGCC 
     3661 AGCGCTGTCG GGCAGTGCGG CCCACACGCC TTTCGCTAGA GGGACTGCAG ACCCTTCCCA 
     3721 GCATGCAAGA GTACCCTCTA CCCACTGAGC CCAAGAGCCC TGGCGAGTAC ATCAACATTG 
     3781 ACTTTGGTGA AGGGGGTACC CGTCTGTCTC CGCCTGCTCC CCCACTACTG GCATCAGCGG 
     3841 CCTCCTCCTC TTCACTGCTC TCAGCCAGTA GTCCTGCTTC ATCCCTGGGT TCAGGTACCC 
     3901 CAGGCACCAG CAGTGACAGC CGGCAGCGCT CTCCGCTCTC TGACTATATG AACCTGGACT 
     3961 TCAGTTCTCC CAAGTCCCCT AAGCCTAGCA CCCGCAGTGG GGACACAGTG GGCTCCATCG 
     4021 ATGGCCTTCT CTCTCCAGAA GCCTCATCCC CATACCCACC GCTGCCCCCG CGTCCTTCTG 
     4081 CTTCCCCTTC CTCCTTACAG CAGCCTCTGC CACCTGCCCC AGGAGACCTA TACCGCCTGC 
     4141 CTCCAGCAAC AGCTGCCACA TCCCAGGGTC CCACTGCTGG CTCCTCAATG TCCTCTGAGC 
     4201 CTGGGGATAA TGGTGACTAT ACCGAGATGG CCTTTGGTGT GGCTGCTACC CCGCCACAAC 
     4261 CTATTGCGGC ACCCCCGAAG CCAGAAGGTG CCCGAGTGAC CAGTCCCACA TCAGGCTTGA 
     4321 AGCGGCTAAG TCTCATGGAT CAGGTATCTG GGGTAGAGGC CTTCCTTCAA GTCAGCCAGC 
     4381 CCCCTGATCC CCACCGGGGC GCTAAGGTCA TCCGTGCAGA CCCGCAGGGG GGACGTCGTC 
     4441 GCCACAGTTC AGAGACCTTC TCCTCTACCA CCACTGTCAC CCCAGTGTCC CCATCCTTTG 
     4501 CTCACAATTC CAAGCGCCAC AATTCGGCCT CTGTGGAAAA CGTCTCTCTC AGGAAAAGCA 
     4561 GTGAAGGCAA CAGCATCCTG GGAGGAAGTG ATGAGCCATC CACATCCCCA GGACAGGCAC 
     4621 AGCCCTCGGC AGGTGTGCCC CCAGCGCCAC AGGCTAGGCC ATGGAACCCC GGTCAGCCTG 
     4681 GAGCTTTGAT TGGCTGTCCT GGAGGCAGCA GTTCTCCCAT GCGCAGAGAG ACCTCCGTGG 
     4741 GTTTCCAGAA CGGCCTCAAC TATATCGCCA TCGATGTGAG AGGTGAGCAG GGGTCCTTGG 
     4801 CGCAGTCTCA GCCTCAGCAT CCACAGCCAG GAGACAAGAA CTCCTGGGGC CGGACCCGTA 
     4861 GCCTTGGGGG GCTCCTCGGT ACCGTTGGAG GCTCTGGCAC CAGTGGAGTG TGTGGGGGTC 
     4921 CAGGCACTGG AGCCTTACCC TCTGCCAGCA CCTACGCAAG CATCGACTTC TTGTCCCATC 
     4981 ACTTGAAAGA AGCCACCGTG GTGAAAGAGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5041 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5101 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5161 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5221 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5281 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5341 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5401 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5461 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5521 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5581 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5641 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5701 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5761 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5821 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5881 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5941 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6001 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6061 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6121 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6181 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6241 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6301 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6361 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6421 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6481 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6541 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6601 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6661 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6721 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6781 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6841 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6901 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6961 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7021 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7081 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7141 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7201 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7261 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7321 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7381 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7441 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7501 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7561 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7621 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7681 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7741 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7801 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7861 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7921 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7981 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8041 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8101 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8161 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8221 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8281 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8341 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8401 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8461 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8521 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8581 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8641 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8701 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8761 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8821 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//