LOCUS       RR217584                8808 bp    DNA     circular  SYN 
DEFINITION  Col18a1 (myc-DDK-tagged) - Rat collagen, type XVIII, alpha 1 (Col18a1)
ACCESSION   NM_053489
VERSION     NM_053489.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4963..5054 
                     /label="Myc-DDK"  
     misc_feature    5084..5669 
                     /label="PolyA signal"  
     misc_feature    5141..5160 
                     /label="Restriction Enzyme"  
     misc_feature    5945..6617 
                     /label="ColE1"  
     misc_feature    7047..7841 
                     /label="Kan/Neo"  
     misc_feature    7876..8178 
                     /label="SV40 ori"  
     misc_feature    8264..8741 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4953..4961 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4953 
                     /label="Col18a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCCAGG TGGCACCTCC TGGACATGCT CACCAGTTTG GTCTTGCTGC 
     1081 TGGTGGCACG CGTCTCCTGG GCAGAGCCAG AGAATGTTGC TGAAGATGTG GGGCTCCTGC 
     1141 AGCTCCTCGG AGACCCCCTA CCCCAGAAGA TCTCCCAAGT CGAAGACCCT CATGTCGGGC 
     1201 TGGCCTACGT CTTTGGACCA TACTCCAAAA GTAGTCAGAT GGCCCAGTAT CACTTCCCTA 
     1261 AACTCTTCTT CCGGGACTTT TCGCTGCTGT TCGAAGTCCG GCCAACCACA GAGGCCGCAG 
     1321 GGGTGCTGTT TGCTATCACA GATGCCGCCC AGGTGGTGGT CTCGCTAGGC GTGAAGCTCT 
     1381 CAGAGGTCCG AGATGGACAA CAGAACATCT CATTGCTCTA CACGGAGCCC GGGGCCAGCC 
     1441 AGACCCAGAC GGGAGCCAGC TTCCGCCTAC CTGCGTTTGT TGGCCAGTGG ACACACTTCG 
     1501 CGCTCAGCGT GGATGGAAGC TCCGTGGCTC TCTACGTAGA CTGTGAAGAG TTCCAGAGAG 
     1561 TGCCGTTCGC TCGGTCCCCA CACGGTCTGG AGCTAGAGCG TGGCGCTGGC CTCTTTGTGG 
     1621 GTCAGGCTGG AGCAGCGGAC CCTGACAAGT TCCAGGGAAT GATCTCAGAG CTGAGAGTAC 
     1681 GCAAAACCCC CCGGGTGAGC CCTGTGCACT GCCTGGATGA AGAAGATGAT GATGACGACC 
     1741 GGGCATCTGG AGATTTTGGA AGTGGCCTCG AAGAAAGCAG CAATTTACAC AGGCAGGAGA 
     1801 CATATCTACG ACCCGGGCTC CCCCAGCCAC CTCCTGTCAC TTCCCCGCCC TTGGCTGGAG 
     1861 GCAGTGCCAC CGAAGATTCT AGGACAGAAG AAAAGGAGGA AGAAGCCACG GTAGATTCTA 
     1921 AAGGAGCTGA TACCCTTCCT GTCACAGATT CAAGCGGTGT GTGGGACGGG GATGTCCAGA 
     1981 ACCCTGGAGG TGGCTTGATA AAGGGAGGTC TCAAAGGACA AAAGGGAGAG CCAGGTGCTC 
     2041 AAGGCCCACC TGGCCCAGCT GGGCCCCAGG GTCCTGCCGG TCCAGCGGTA CAGAGCCCCA 
     2101 GCTCACAACC TGTCCCTGGA GCACAAGGAC CCCCGGGACC TCAGGGACCA CCAGGGAAGG 
     2161 ATGGCATCCC GGGAAGGGAT GGTGAACCAG GCGACCCTGG TGAAGACGGG AGGCCGGGTG 
     2221 ACACTGGACC TCAAGGCTTC CCGGGGACCC CAGGAGATGT GGGCCCTAAG GGTGAGAAGG 
     2281 GAGATCCTGG TATTGGGCCT CGAGGACCTC CAGGACCTCC GGGACCACCA GGACCCTCCT 
     2341 TCAGACAGGA CAAGCTGACC TTCATTGACA TGGAGGGATC TGGTGGTTTC AGCGGAGACC 
     2401 TAGAGAGCCT CAGAGGCCCA CGAGGCTTCC CTGGTCCCCC AGGTCCCCCT GGTGTCCCAG 
     2461 GACTGCCTGG TGAACCAGGG CGCTTTGGAG TCAACAGTTC CTACGCACCA GGACCTGCAG 
     2521 GCCTTCCTGG TGTGCCTGGG AAGGAAGGGC CCCCTGGTTT TCCTGGTCCC CCAGGACCTC 
     2581 CAGGCAAAGA GGGCCCACCA GGAGTGGCCG GCCAGAAAGG CAGTGTTGGC GATGCAGGCA 
     2641 GCCCAGGACC CAAGGGGAGC AAAGGAGACC TTGGGCCCAT TGGTATGCCT GGGAAGTCTG 
     2701 GCTTGCCGGG ACTCCCTGGG CCAGTTGGAC CCCCAGGACC TCCAGGGCCT CCAGGGCCAC 
     2761 CAGGACCAGG ATTTGCTGCT GGATTTGATG ACATGGAAGG TTCTGGAACA CCTCTCTGGT 
     2821 CAACAGCCCG AAGCTCCGAT GGGCTGCAGG GGGATCCTGG AGTGACTGGG CCACCTGGGG 
     2881 CCAAGGGAGA AGTTGGAGCA GACGGAGTCC AGGGCATCCC TGGTCTCCCA GGAAGAGAGG 
     2941 GTGTAGCTGG GCCCCCGGGA CCCAAAGGAG AGAAAGGGAC CCAGGGAGAA AAGGGAAACC 
     3001 CAGGAAAAGA TGGAGTAGGT CGGCCAGGCC TCCCTGGCCC TCCAGGCCCC CCAGGGCCTG 
     3061 TGATCTATGT GTCAAATGAG GATAGAGCTG TAGTAAGCAC GCCGGGACCT GAGGGCAAGC 
     3121 CAGGGTACGC AGGCTTTCCT GGACCTGCTG GACCGAAGGG TGACCTGGGT TCCAAAGGCG 
     3181 AGCAGGGTCT TCCAGGGCCC AAGGGTGAGA AGGGAGAACC AGGCAGTATC TTTAGCCCTG 
     3241 ATGGCACAGC CCTGGGCCAG GCCCAGAAGG GAGCCAAGGG AGAACCAGGC TTCCGAGGAC 
     3301 CCCCGGGTCC TTATGGACGG CCTGGGTACA AGGGTGAAAT TGGCTTCCCT GGACGGCCGG 
     3361 GTCGTCCCGG AACAAATGGC TTAAAGGGAG AGAAAGGGGA GCCTGGAGAG GCCAGCCTTG 
     3421 GGTTCAGCAT GAGGGGACTG CCTGGCCCCC CTGGGCCTCC AGGCCCCCCA GGTCCTCCCG 
     3481 GAGTGCCCGT CTATGACAGC AATGCATTTG TGGAGTCTGG CCGACCTGGA CTACCAGGAC 
     3541 AGCAAGGTGT GCAGGGGCCT CCAGGACCAA AGGGTGACAA AGGAGAGGTG GGCCCACCCG 
     3601 GGCCACCAGG GCAGTTCCCC ATTGACCTCT TCCACCTGGA AGCCGAAATG AAGGGGGACA 
     3661 AGGGAGACCG AGGGGATGCT GGACGGAAGG GAGAGAGGGG AGAGCCTGGG GCTCCTGGTG 
     3721 GTGGATTCTT CAGCTCAAGT GTACCTGGAC CACCTGGCCC ACCTGGATAC CCTGGAATTC 
     3781 CGGGTCCAAA AGGAGAGAGC ATTCGGGGTC CGCCTGGCCC TCCTGGCCCG CAGGGACCTC 
     3841 CTGGCATTGG ATATGAGGGG CGCCAGGGCC CCCCAGGACC TCCAGGACCT CCAGGACCTC 
     3901 CCTCATTCCC TGGCCCTCAT AGACAGACTG TCAGTGTTCC TGGTCCTCCA GGCCCACCTG 
     3961 GCCCCCCAGG TCCCCCAGGA GCCATGGGTG CCTCTGCTGG GCAGGTAAGG ATCTGGGCTA 
     4021 CATACCAGAC CATGTTGGAC AAGATACGTG AGGTGCCAGA GGGCTGGCTC ATCTTTGTGG 
     4081 CCGAGAGGGA AGAGCTCTAC GTACGGGTTA GAAATGGCTT CCGTAAGGTG CTGCTGGAGG 
     4141 CCCGGACAGC CCTCCCGCAC GGGACGGACA ACGAGGTAGC TGCCTTGCAA CCTCCATTGG 
     4201 TACAGCTTCA TGAGGGCAGT TCATATACCC GGAGGGAGCA CTCCTATCCC ACGGCACGAC 
     4261 CCTGGCGAGC AGATGACATC TTGGCCAACC CACCACGTCT GCCGGACCGC CAGCCTTACC 
     4321 CGGGAGTTCC ACACCACCAC CACCACCACC ACCACCACCA CAGTTCTCAT GAACACCGGC 
     4381 CGCCAGCCCA CCCCTCCCCC TCACCTGCCC ATACTCACCA GGACTTTCAC CCAGTGCTCC 
     4441 ACCTGGTGGC ACTGAACACC CCTCTGTCTG GAGGCATGCG TGGTATCCGT GGGGCTGACT 
     4501 TCCAGTGCTT CCAGCAAGCC CGAGCCGTGG GGCTGTCTGG CACCTTTCGG GCCTTCCTGT 
     4561 CCTCTAGGCT GCAGGACCTC TACAGCATTG TACGCCGTGC TGACCGCAGC TCTGTGCCCA 
     4621 TCGTCAACCT GAAGGATGAG GTACTATCTC CCAGCTGGGA CACCCTGTTC TCCGGCTCCC 
     4681 AGGGTCAACT GCACTCTGGG GCCCGCATCT TTTCTTTCGA CGGCAGAGAC GTCCTGAGAC 
     4741 ACCCAGCCTG GCCACAGAAG AGCGTATGGC ATGGCTCAGA CCCCAGTGGG CGCAGGCTGA 
     4801 TGGAGAGCTA CTGTGAGACG TGGAGGACTG AAGCTACTGG GGTTACAGGT CAGGCCTCTT 
     4861 CCCTGCTGTC GGGCAGGCTC CTGGAACAGA AGGCCGAGAG CTGCCACAAC AGCTATATCG 
     4921 TCCTGTGCAT TGAGAATAGC TTCATGACCT CTTTCTCCAA AACGCGGCCG CTCGAGCAGA 
     4981 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5041 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5101 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5161 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5221 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5281 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5341 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5401 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5461 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5521 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5581 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5641 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5701 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5761 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5821 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5881 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5941 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6001 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6061 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6121 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6181 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6241 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6301 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6361 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6421 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6481 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6541 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6601 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6661 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6721 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6781 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6841 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6901 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6961 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7021 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7081 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7141 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7201 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7261 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7321 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7381 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7441 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7501 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7561 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7621 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7681 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7741 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7801 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7861 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7921 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7981 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8041 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8101 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8161 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8221 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8281 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8341 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8401 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8461 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8521 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8581 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8641 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8701 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8761 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//