LOCUS       RR217577                8754 bp    DNA     circular  SYN 
DEFINITION  Cachd1 (myc-DDK-tagged) - Rat cache domain containing 1 (Cachd1)
ACCESSION   NM_001191758
VERSION     NM_001191758.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4909..5000 
                     /label="Myc-DDK"  
     misc_feature    5030..5615 
                     /label="PolyA signal"  
     misc_feature    5087..5106 
                     /label="Restriction Enzyme"  
     misc_feature    5891..6563 
                     /label="ColE1"  
     misc_feature    6993..7787 
                     /label="Kan/Neo"  
     misc_feature    7822..8124 
                     /label="SV40 ori"  
     misc_feature    8210..8687 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4884..4891 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4884 
                     /label="Cachd1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCGCGAG CCCGAGGAAG AGGAGACCGT AAGGACGGCC GCTCTGGTCC 
     1081 GTCGCTGTCC CCGCTGCCCT GGCTGGCCTG GGGCCCCGCG GCCGCCCTTC TGGCTGCTCT 
     1141 GCCTTGTGGC ATGCTGGATC TTGGGCGCCG TGGCGGACGC CGACTTCTCC ATCCTGGACG 
     1201 AGGCGCAAGT GCTAGCGAGC CAGATGCGGA GGCTGGCTGC CGAGGAGCTG GGGGTGGTCA 
     1261 CCATGCAGAG GATCTTCAAC TCCTTGGTTT ACACAGAGAA GATCTCAAAC GGAGAGAGCG 
     1321 AAGTTCAGCA GCTAGCCAAA AAAATCCGGG AGAAGTTCAA CCGTTACCTG GACGTGGTCA 
     1381 ATCGGAACAA GCAAGTTGTA GAAGCCTCCT ATACAGCCCA CCTGACCTCC CCGCTGACCG 
     1441 CCATCCAGGA CTGCTGCACA ATTCCACCCT CCATGATGGA GTTTGATGGG AACTTCAATA 
     1501 CCAATGTGTC TAGAACAGTG AGCTGCGATC GACTCTCTAC TACTGTGAAC AGCCGAGCCT 
     1561 TCAATCCAGG ACGAGACTTA AATTCAGTTC TTGCAGACAA CCTGAAATCT AACCCTGGAA 
     1621 TCAAGTGGCA ATATTTCAGC TCAGAAGAAG GAATTTTCAC TGTCTTCCCA GCACACAAGT 
     1681 TCCGGTGTAA GGGCAGCTAT GAGCACCGCA GTCGGCCCAT CTATGTCTCT ACCGTCCGGC 
     1741 CGCAGTCCAA ACACATAGTC GTGATCCTGG ACCACGGGGC CTCAGTCACG GACACCCAGC 
     1801 TCCAGATTGC CAAGGACGCC GCTCAGGTCA TCCTCAGTGC CATTGATGAA CACGACAAGA 
     1861 TTTCTGTATT GACTGTGGCA GACACCGTCA GGACCTGTTC GTTGGACCAG TGCTATAAGA 
     1921 CGTACCTGTC TCCAGCCACC AGTGAGACAA AAAGGAAAAT GTCCACATTT GTTAGCAGTG 
     1981 TCAAACCCTC CGACAGCCCC ACCCAGCACG CAGTAGGGTT TCATAGGGCG TTCCAGTTGA 
     2041 TTCGAAGCAC CAGCAATAAC ACTCGGTTCC AAGCAAACAC AGACATGGTC ATCATTTACC 
     2101 TGTCAGCGGG TATCACATCA AAGGACTCCT CGGAAGAGGA TAAAAAGGCA ACTCTCCGCG 
     2161 TCATCAACGA AGAAAATGGC TTCCTGAACA ACTCTGTAAT GATTCTTACC TACGCCCTCA 
     2221 TGAATGATGG GGTGACTGGT TTGAAGGAAC TGGCGTTCCT CAGGGATCTG GCTGAACAGA 
     2281 ACTCTGGGAA GTATGGAATC CCAGACCGGA CGGCCTTGCC TGTGATTAAG GGCAGCATGA 
     2341 TGGTGCTGAA CCAGCTGAGC AACCTGGAGA CCACTGTTGG CAGGTTCTAT ACCAACCTTC 
     2401 CCAACCGGAT GATTGACGAG GCCGTCTTCA GCCTCCCCTT CTCTGATGAG ATGGGAGATG 
     2461 GTCTGATCAT GACTGTGAGT AAACCCTGTT ACTTTGGAAA CCTTCTCCTG GGGATCGTAG 
     2521 GTGTGGATGT GAATCTGGCT TACATTCTCG AAGATGTGAC ATATTACCAA GACTCTTTAG 
     2581 CTTCCTATAC TTTTCTCATA GATGACAAAG GGTACACACT TATGCACCCG TCTCTCACCA 
     2641 GGCCATACTT ACTGTCAGAG CCTCCCCTTC ATACCGACAT CATACATTAT GAAAATATCC 
     2701 CCAAGTTTGA ATTGGTTCGA CAAAATATCC TAAGCCTCCC TCTAGGCAGC CAGATCATCA 
     2761 CAGTGCCTGT TAACTCGTCC CTGTCTTGGC ACATCAACAA GCTGAGGGAG ACTGGAAAGG 
     2821 AGGCCTACAA TGTGAGCTAT GCCTGGAAGA TGGTACAGGA CACTTCCTTC ATTCTGTGTA 
     2881 TTGTGGTGAT ACAACCAGAA ATACCTGTCA AACAATTGAA GAACCTCAAC ACCGTACCCA 
     2941 GCAGCAAGCT GCTGTACCAC AGGCTAGACC TTCTGGGCCA ACCCAGTGCT TGTCTCCACT 
     3001 TCAAGCAGCT GGCAACACTA GAAAGTCCAA CCGTCATGCT GTCTGCCGGC AGCTTTTCCT 
     3061 CCCCCTATGA GCACCTCAGC CAGCCAGAGA CCAAGCGCAT GGTGGAGCAT TACACGGCCT 
     3121 ATCTCAGTGA CAACACTCGC CTCATTGCTA ACCCAGGGCT CAAATTCTCT GTCAGAAACG 
     3181 AAGTAATGGC GACCAGCCAC GTCACAGATG AATGGATGAC ACAAATGGAA ATGAGCAGCC 
     3241 TGAACACGTA CATTGTCCGC CGTTACATAG CAACACCCAA TGGCGTCCTC AGAATTTATC 
     3301 CTGGTTCCCT CATGGACAAA GCATTTGATC CCACGAGGAG ACAGTGGTAT CTTCATGCAG 
     3361 TAGCCAATCC AGGGCTGATT TCCTTGACGG GCCCTTACCT AGATGTTGGA GGAGCTGGCT 
     3421 ATGTCGTGAC AATCAGCCAC ACAATTCACT CATCCAGTAC CCAGCTGTCT TCCGGACACA 
     3481 CTGTGGCTGT GATGGGCATC GACTTCACAC TGAGGTACTT CTACAAGGTT CTGATGGATC 
     3541 TCTTACCAGT TTGTAACCAA GATGGTGGCA ACAAAATAAG GTGCTTCATA ATGGAGGACA 
     3601 GAGGTTATCT GGTAGCTCAT CCAACCCTCG TTGACCCCAA AGGTCATGCA CCTCTGGAAC 
     3661 AGCAACACAT CACCCACAAG GAGCCCCTGG TAGCAAACGA TATCCTGAAC CATCCCAACT 
     3721 TTGTGAAGAA GAACTTGTGC AACAGCTTCA GTGACCGGAC AGTGCAGAGA TCTTACAAGT 
     3781 TCAACACCAG CCTGGTGGGG GATTTGACAA ACCTTGTGCA TGGCAGCCAC TGTTCTAAAT 
     3841 ACCGCCTGAC AAGGATCCCA GGAACCAACG CGTTTGTCGG CATCGTCAAT GAGACCTGCG 
     3901 ACTCTCTTGC CTTCTGCGCT TGCAGCATGG TGGACCGTCT CTGTCTCAAC TGTCACCGAA 
     3961 TGGAGCAAAA TGAATGTGAA TGTCCTTGTG AGTGCCCCCT GGAGGTCAAT GAGTGTACTG 
     4021 GCAACCTCAC CAATGCAGAG AACAGAAACC CGAGCTGTGA GGTCCACCAG GAGCCAGTGA 
     4081 CATACACAGC TATCGATCCT GGCCTGCAAG ATGCCCTGCA CCAGTGTGTC AACAGTCGAT 
     4141 GTAACCAGAG GATGGAAAGT GGGGACTGTT TCGGAGTGCT GGACTGCGAG TGGTGCGTGG 
     4201 TGGACAGTGA CGGAAAGACT CACCTGGACA AATCCTACTG TGCGCCCCAG AAAGAATGCT 
     4261 TTGGAGGGAT CGTGGGAGCC AAAAGTCCCT ACGTTGATGA CATGGGAGCC ATCGGTGATG 
     4321 AGGTGATCAC ACTGAACATG ATTAAGAGCG CCCCAGTGGG CCCTGTGGCC GGAGGGATCA 
     4381 TGGGCTGCAT CATGGTGCTG GTCCTTGCCG TCTACGCTTA CCGCCACCAG ATCCATCGCC 
     4441 GGAGCCACCA GCATATGTCT CCTCTTGCTG CTCAAGAAAT GTCAGTGCGT ATGTCCAACC 
     4501 TGGAGAATGA CAGAGATGAA AGAGAGGATG ACAGTCACGA AGACAGAGGC ATCATCAGCA 
     4561 ACACTCGGTT CATAGCTGCA GTCATGGAGC GGCACGCACA CAGCCCGGAA AGGAGGCGAC 
     4621 GCTACTGGGG CCGCTCGGGA ACCGAAAGTG ATCATGGGTA CAGCACCATG AGCCCGCAGG 
     4681 AAGACAGTGA GAACCCTCCA TGCAACAATG ACCCTTTGTC AGCTGGGGTG GATGTAGGGA 
     4741 ACCATGATGA CGACTTGGAC CTGGACACCC CGCCTCAGAC TGCAGCCCTG CTGAGCCACA 
     4801 AGTTCCACCA CTACCGCTCA CACCACCCGA CCCTCCATCA CAGCCACCAC CTGCAGGCGG 
     4861 CCGTGACAGT GCACACGGTG GATGCTGAGT GCAGCGGACC GACGCGTACG CGGCCGCTCG 
     4921 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4981 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5041 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5101 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5161 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5221 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5281 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5341 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5401 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5461 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5521 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5581 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5641 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5701 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5761 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5821 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5881 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5941 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6001 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6061 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6121 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6181 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6241 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6301 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6361 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6421 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6481 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6541 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6601 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6661 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6721 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6781 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6841 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6901 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6961 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7021 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7081 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7141 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7201 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7261 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7321 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7381 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7441 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7501 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7561 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7621 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7681 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7741 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7801 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7861 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7921 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7981 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8041 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8101 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8161 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8221 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8281 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8341 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8401 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8461 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8521 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8581 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8641 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8701 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//