LOCUS       RR217575                8724 bp    DNA     circular  SYN 
DEFINITION  Plekha5 (myc-DDK-tagged) - Rat pleckstrin homology domain containing, family A member 5 (Plekha5)
ACCESSION   NM_139340
VERSION     NM_139340.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4881..4972 
                     /label="Myc-DDK"  
     misc_feature    5002..5587 
                     /label="PolyA signal"  
     misc_feature    5059..5078 
                     /label="Restriction Enzyme"  
     misc_feature    5863..6535 
                     /label="ColE1"  
     misc_feature    6965..7759 
                     /label="Kan/Neo"  
     misc_feature    7794..8096 
                     /label="SV40 ori"  
     misc_feature    8182..8659 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4863..4869 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4863 
                     /label="Plekha5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCGAC CTGAACCTGG AGTGGATTTG TTCGCTGCCG CGCTCCTGGA 
     1081 CGTACGGGAT CACTAGGGGC GGCCGCGTCT TCTTCATCAA CGAGGAGGCC AAGAGCACCA 
     1141 CCTGGCTGCA CCCGGTCACC GGCGAGGCCG TGGTCACCGG GCACCGGCGG CAGAGCACAG 
     1201 ATTTGCCCAC TGGCTGGGAA GAAGCATATA CATTTGAAGG CGCAAGATAC TATATAAACC 
     1261 ACAATGAAAG GAAAGTAACC TGCAAGCATC CAGTCACAGG ACAGCCATCC CAGGACAACT 
     1321 GTATCTTTGT CGTGAATGAC CAGACTGTTG CAACCATGAC ATCTGAAGAA AAGAAGGAGC 
     1381 GACCCATAAG TATGATAAAT GAAGCTTCCA ATTACAACAT GGCTTCAGAC TATACTGTGC 
     1441 ATCCTATGAG CCCCGTGGGC AGGACATCAC GGGCCTCCAA AAAGGTTCAT AATTTTGGGA 
     1501 AAAGGTCCAA TTCAATTAAA AGGAACCCTA ATGCCCCTGT GGTCAGGCGC GGCTGGCTGT 
     1561 ATAAGCAGGA CAGCACCGGC ATGAAGCTGT GGAAGAAGCG CTGGTTTGTG CTTTCTGACC 
     1621 TTTGCCTTTT TTACTACAGA GATGAGAAGG AGGAGGGTAT TTTGGGAAGC ATCCTGCTGC 
     1681 CCAGTTTTCA GATAGCCATG CTTACTGCCG AGGACCACAT CAATCGCAAA TATGCTTTTA 
     1741 AGGCAGCCCA CCCAAACATG CGGACCTATT ATTTCTGCAC TGATACAGGC AAGGAAATGG 
     1801 AGCTATGGAT GAAGGCCATG TTAGATGCTG CTCTGGTGCA GACAGAACCT GTGAAAAGAA 
     1861 TTACCTTTAA TTTCCGAGTG GACAAGATTA CAACAGAAAG TGCATCAACT AAAGAAACCA 
     1921 ATAACATTCC CAACCACAGA GTCCTCATTA GACCTGAGGT CCAAAACAAT CAGAAAAATA 
     1981 AGGAAATAAG CAAAATTGAA GAAAAAAGGG CCTTAGAAGC TGAAAGATAC GGGTTTCAGA 
     2041 AGGATGGGCA AGATAGACCA TTAACAAAAA TTAACAGTGT AAAGTTGAAC TCGCTGCCAT 
     2101 CAGAATATGA AAGCGGGCCA GCCTGCCCAC CTCAGAATAT ACACTACAGA CCCATCAATG 
     2161 TGAATAATTC TGACAGCAAA GCAGTGAATG TCAGCTTGGC AGATGTCCGG GGTGGGAGTC 
     2221 ACCCAAATAC AGGGCCCCTA GCCACAGAAG CTGATCGAGT CATTCAGAGA ACGAATTCAA 
     2281 TGCAGCAGCT GGAGCAGTGG ATTAAAGTTC AGAAAGGACG GGGTCTTGAA GAGGAGCCCA 
     2341 GAGGAGTAAT TTCTTATCAA ACATTGCCAA GAAATATGCC AAGCCACCGA GCCCAGATTC 
     2401 TGGCCCGCTA CCCTGAAGGT TACCGAACAC TCCCAAGAAA CAGCAAGACC AGACCAGAGA 
     2461 GTATCTGCAG TGTAACGCCT TCTGGTCATG AGAAGACAGG GCCTGGGGCA GAGGAGAAGC 
     2521 GGCGGTCCAT GAGGGATGAC ACCATGTGGC AGCTGTATGA GTGGCAGCAG CGCCAGTTTT 
     2581 ACCACAAGCA GAGCACCCTC CCTCGGCACA GCTGCCTGAG CAGCCCTAAA GCCATGGGGA 
     2641 AGGTTTCTGA CCAGACAATG CACTCCATCC CCACGTCACC GTCCCATGTG CCAGCTGCTG 
     2701 CTTACCAGGG CTTCTCCCCT CAGCGGACAT ACAGGTCAGA AGTGTCCTCG CCAATCCAGA 
     2761 GAGGAGATGT GACAATAGAC CGCAGGCACA GGCCTCATCA CCCTAAGCAT GTCTATGTAC 
     2821 CTGACAGAAG GTCAATGCCG GCTGGCTTAG CCTTACAGGC TGTTAGTCCT CAGAGCCTCC 
     2881 ACGGCAGGAC GCCAGAGGAG CTTACGCTGC TGCTGATAAA GCTGAGACGG CAGCAAGCAG 
     2941 AGCTGAGTAG TATCCGGGAG CACACCTTAG CACAGCTCAT GCAGCTGAAA CTCGAGGCCC 
     3001 ACAGCCCGAA GAATGAAATT CTTTCACATC ATCTTCAAAG GAACACCATA TATTTGGATC 
     3061 ATCAGATGAA AGAAAATGAA CCTATTATCA CCATGGTTCA CACCATGATT GAGAACTCGG 
     3121 CGCTAAGACC ACAACTGTAC CAACAATTCT TAAGACAGAA GAACAAGATA AGTCTCTATT 
     3181 GTCTCTCACA AGATGAGTGT AGAGGCACAT TATACAAATA CAGACCTGAG GAAGTAGATA 
     3241 TCGATGCCAA ATTAAGCCGA TTGTGTGAAC AGGATAAGGT GGTCCGTGCA CTGGAGGAGA 
     3301 AGCTGCAGCA GCTGCACAAG GAGAAATACA CGCTTGAGCA AGCCTTGCTG TCAGCCAGCC 
     3361 AAGAGATAGA GATGAATGCA GATAACCCAG CCGCCATTCA GACGGTGGTG CTGCAGAGGG 
     3421 ACGACCTGCA GAACGGGCTG CTGAGCACGT GTCGGGAACT CTCCCGAGCC ACCGCTGAGC 
     3481 TGGAACGAGC CTGGAGAGAA TATGACAAGT TAGAATATGA TGTGACTGTT ACCAGGAACC 
     3541 AGATGCAAGA TCAGCTTGAT CGCCTTGGAG AAGTGCAGAG TGAGTCGGCA GGAATTCAAC 
     3601 GGGCACAGAT TCAAAAAGAA CTTTGGAGAA TTCAAGATGT CATGGAGGGG TTGAGCAAAC 
     3661 ACAAACAGCA AAGAGGCTCT TCAGAAACAG GTCTGGCAGG ACCAAAGCCT TTCCCAGCAG 
     3721 TTAAGTACAA AAGCGAGGAA GAGGAAGTAG TCCCACCTCG GCCTCCACTT CCTCGGTCTT 
     3781 ATGACTTTAC AGAGCAGCCT CCCATAATCC CCCCTCTGCC CAGTGATAGC AGCTCCTTGC 
     3841 TCTGTTATAG CAGGGGCCCC GTGCATCTGC CTGAAGAAAA GAAGAGTCAC CAAGTTCAAG 
     3901 GATATCCAAG AAATGGATCT CACTGTGGTC CAGATTATAG ACTCTACAAG AGCGAGCCAG 
     3961 AGTTGACCAC AGTGGCAGAA GTTGATGAAT CTAATGGAGA AGAAAAATCA GAGCCAGCTT 
     4021 CTGAGGCAGA AGCTCCGGTT GTTAGAGGTT CCCACTTTCC AGTTGGAGTT CCTCTGAGAA 
     4081 CGAAGTCACC TACACCTGAG TCCTCAACGA TAGCTTCCTA TGTGACCTTG AGGAAGACGA 
     4141 AGAAGATGGT GGAGCTAAGA ACGGAAAGAC CGAGAAGTGC CGTGGAACAG CTCTGTCTGG 
     4201 CGGAAAGTAC TCGGCCTCGG ATGACTGTGG AAGAGCAGCT GGAGAGAATC AGGAGGCACC 
     4261 AGCAGGCCTG CCTGAGGGAG AAGAAGAAGG GGCTGAACGT CCTCAGTGCA TCCGACCCAT 
     4321 CTCCCTCACA AAGTCCTTCA AGCGTGAGGG ACAGTCCTTC CAGAGTTCCT CAGACTGTAA 
     4381 GGAGGGATGA TAACACTAAG GAGCTAGACG CCGTCCACAG AGAAAATGAT GGAAAGCCAG 
     4441 ACTATGACAC TTCAGCAGCA GAAACTGCTC ACCCAGAAGA AGCTGAACCC CAAAGTGCAG 
     4501 ATGTCGGCCG AAAGCTAAAG AGAACTGAAA GTATTTTTTA TGAAATGCTT TACACGCCTG 
     4561 AACCTTTAAA TGGACTGACT TCTGAGGAAG TGATGGCTAA GGAGAGGCAG GAAGAGCAGG 
     4621 TGCCTGAAGG TGCCGTGTGC AGCCTTCAGG AAGAGGCTGC CATGGCAAAC CATCAGGCAG 
     4681 AAGGCCCCCC TGAGGAAGCA GAGAGTCTTC ATGAAGAGGA GGAAGCTCTG GCTTCCTGTG 
     4741 AGCCAGCTCC TGAGACTCCT AGGGAGAATC AAACCACAGT GAAAAGTCTG TCTCCGTCTC 
     4801 CTGACTCCTC CACGGCAGCA GACCCGCCCA CTCCGCCCCA GCTCACAGAA GGATCACATT 
     4861 TCATGTGTGT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4921 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4981 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5041 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5101 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5161 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5221 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5281 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5341 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5401 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5461 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5521 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5581 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5641 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5701 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5761 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5821 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5881 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5941 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6001 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6061 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6121 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6181 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6241 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6301 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6361 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6421 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6481 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6541 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6601 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6661 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6721 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6781 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6841 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6901 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6961 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7021 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7081 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7141 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7201 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7261 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7321 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7381 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7441 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7501 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7561 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7621 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7681 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7741 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7801 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7861 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7921 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8041 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8161 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8221 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8281 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8341 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8401 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8461 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8521 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8581 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8641 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8701 TAACAAAAAT TTAACGCGAA TTTT
//