LOCUS       RR217573                8718 bp    DNA     circular  SYN 
DEFINITION  Mdc1 (myc-DDK-tagged) - Rat mediator of DNA damage checkpoint 1 (Mdc1)
ACCESSION   NM_001166275
VERSION     NM_001166275.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4875..4966 
                     /label="Myc-DDK"  
     misc_feature    4996..5581 
                     /label="PolyA signal"  
     misc_feature    5053..5072 
                     /label="Restriction Enzyme"  
     misc_feature    5857..6529 
                     /label="ColE1"  
     misc_feature    6959..7753 
                     /label="Kan/Neo"  
     misc_feature    7788..8090 
                     /label="SV40 ori"  
     misc_feature    8176..8653 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4857..4863 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4857 
                     /label="Mdc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAAACACT CAGGTGATTG ACTGGGATGC TGAAGAAGAG GAAGAGACAG 
     1081 AAATATCCAG TGGATCCTTG GGGTATAGTG TGGAGCCTAT AGGGCGGCTG CGATTCTTCA 
     1141 GTGGTACCCA TGGACCAGAA CGAGATTTCC CACTCTACCT TGGCAAGAAT GTAGTTGGTC 
     1201 GAAGCCCTGA CTGCTCTGTG GCCCTGCCTT TTCCATCCAT CTCCAAACAG CACGCAGTGA 
     1261 TTGAAATCTC AGCTTGGAAC AAAGCCCCTA TCCTTCAGGA TTGTGGGAGC CTCAATGGCA 
     1321 CTCAAATCGT AAAGCCTCCT AGGGTCCTAG CTCCTGGAGT GAGCCATCGT CTGAGGGACC 
     1381 AGGAATTAAT TCTGTTTGCA GACTTTCCCT GCCAGTACCA TCGCCTGAAT GTCCCACCAC 
     1441 CCTTGGTCCC TCGGAGTCTT CTAACTATAG AGAAGACCCC CAGAATACGG GGAAGATCCC 
     1501 AAAATTCCAG AGTTTTGTTG GCTGAGGATT CAGAGGAAGA AGGAGATTTT CCTTCTGGAA 
     1561 GGTCTGTGGC AAATGGATCA AGGAATACAG CATCCCCTTC AGCAACAGTA GTTCCAGAAA 
     1621 GTGATGAAGA GGGCTCTTCT CCAGGCCCAA GTGTCCCTGG GCCATCTTCA CCCTTTGGTT 
     1681 TGGGAAGTGA CACAGATGAA TCACAAGGTC AGCAACCAGG AGTAGAGGAA TCTTCTTTAG 
     1741 CTGACAACAG TGGTGCCGCA GGGGAGCCTG AGCAGCCTGA AGTGAATGGA GTGACAACTG 
     1801 GCACACTGGC TCAGCCTACC AAGGACAAGT TCAAGGACAC AAAGATGAAG GAGGAGGCAG 
     1861 GCAGTGCAGG GGTTCCAGTT GGGTCAGTTG TGGAGGGGAG CCCCACTCTT GGGGAAGACA 
     1921 GTGACACAGA AGCGGATGAA GAGCGTCAGC CTTCTGGTTC TGGGGATAGT GATACGGACG 
     1981 TAGAAGAAGA GAGGGTCCCT GTGAAGAAGA ACCAAGTACT GCTTGGGGTT GGTATAGGGG 
     2041 GTCCAGGAGC ACGTGGTGTG GCACATCTGC AGGACAGCCC AACTGGTAGT GACACAGATG 
     2101 TGGAGGAGGA CAAGACTGCA CTGGCTGCCC CTCCAGAGAG AAGCCACACA GCCATGGTGA 
     2161 TCAACAGTGA TACAGACGAG GAGGAGAGGG GTGAGGAGGA AGAGGTCTCG GCAGCACTCA 
     2221 CCTTGGCCCG TCTGAAGGAG AGAGGAATTG CTTTGTGGAG TGGAGAGCCA GGCACAGAGG 
     2281 AGGTCAAGTC CCAACCACAG GTCCTTGTAG AACGAAGCCA GAGTGCCTCT GGGAGAGACA 
     2341 GTGACACTGA CGTGGAGGAG GGATCCTCAG GGGGAAAGAG AGAGATTGTC CCTGACAGCC 
     2401 CCATGGATGT AGATGAGACT CTTACTGTCA CACAGCCAGA GAGCCAGCCT CCCTGTAGGC 
     2461 CCAATGATGT AGACGAGGAT GTGGATATGA GCTCCCCTGG CAGCCATCTA GAGGGAAAGA 
     2521 AGGCCTCCTC TGCTCTGGTA GACAAGAACA GAGCACAAGT GGAGGAGGAA GTCCCAGGGC 
     2581 CATCTGTTAC CCTGGGGGAG AAACACCAGG TGCCTCTAGA GGGAGCCCAG CCTCCTGAGG 
     2641 AAGCCCGGGA AACAGCTGTG CAGGAAGGCT CATCCTCACC AGTGGCAGAC ATAAGAATGA 
     2701 GCCAGCAGCC AGTAGCGGAA GATGCTGGGA CAGAGTGTGC TGCAGCTGTT TCTGAACAGA 
     2761 AAAGTGCTCT TGAGGTCGGG GCTCAGAGCA GGTCACCTGC AGCACCCGTG GAGCAGGTGG 
     2821 TGGTACGCAC AGATACTTCA GGGGATCCTA CCCTGCCACA GAGAGAGGGA GCCCAGACCC 
     2881 CCACAGGAAG GGAAAGAGAA GCACATGTGG GCGGGACCAA GCATGCCAAA GAGTGCTGTG 
     2941 ATGAACCTGA GGATCTGTGC CTTTCAGCCA CCCAGTGCTT TGTGGAAGGG GAGAGCCAGC 
     3001 ACCCAGGAGC TGTCCAGAGT TTGGAAGATG AGCCTACCCA AGTCTTCCCA TGCCTTCCCC 
     3061 AAGAGCCTGG ACCTTCCCAT CTCAGCCTCC CGACTCCAGG TGCAGATACC TTGGATGTGC 
     3121 CTTGGGAAGT CTTGGCTACA CAGCCGTTCT GTCTGAGAGA ACAGACAGAG ACCTCTGAGC 
     3181 CCATTGACAC CCATGAAGCT CATGGATCTC AACCATCTCT ACCTGGGGAA CCACCAGGAC 
     3241 ACCAACATCC GGTCCCTACC AGCCTAGATC ACACAGAGCT GTTGAGAATT GATGACAGAG 
     3301 AGATGCAGAC TGTGGAGAAA GCCATGGGCC ACCTAAGTTG CCAGATGATG CCAGATGGAA 
     3361 AGGCTTCCGG GGATGACCCA GAGCCCTCAG ACCATCGCCT GTTTTCTCCA GTGCCTGAAG 
     3421 CTTCAGCTTC ACCTCAGAGT CTCCTCACCT CTCAGAGCCA AAAGCAATCT ACACCTCAGC 
     3481 CTATGTTTCC CACTTCTTCT TCTGAGCTAG CCCTTCCTGA GACTCTTCAC ACAAAGCCTA 
     3541 ATGTCAGGCC ACGGCGGTCC TCCAGGATGA CCCCCTCCCC ACATTCCTCT GCTGCCCTTA 
     3601 AGCCTTATAC TACCTGCCCC ACAAACCAGC CTGCTGCTTC TAGGCCAACA TCTCGCCCCA 
     3661 CTCGGGGCAG GGCAAATAGG TCCTCTACCA GGACCCCAGA ACTGATTGTC CCTACAGGCC 
     3721 CTGAGCTCCA GCCTTCCACC TCCACAGAAC AGCCTGGCAT TCCAAACCTC ACTTCTCAGG 
     3781 TCACTGAGGG CAGGGCACAT AGTACCTCTG TTAATATGCC TGAACCAGTT CTCACAGGTC 
     3841 CCGAAGCCCA GCCTCTCACC TCCGCAGAAC AATCTGTCAC CTCAAACCTC AATCCTCGGG 
     3901 CCCAGCCTCT TACCCTGGAG CCAGTTCCAC AAACCAGTCA CCAAAGGAGG CGAAGGGCTA 
     3961 CTGGGAAGCA GGGCTCCCGC ACAGCTCCTG TTGGTCCTAA GTCTTACTCT ACCCCCGCTG 
     4021 AACCTGAACC CCAGTCTTCA GCCAGCCAAA GCTCAGGAGC ATCAGAAGCA GATTCACCCC 
     4081 ATCAAAAACG TCCCCGAAGA CAAGTCACCC AGAAAACTGT AGTCGTCAAG GAAGAAGATC 
     4141 CTGGAGAAAT ACAAGTGAAG GAAGAGCCTC AGGAGACAGC AATTCCAACA CCAGGCAAGA 
     4201 GAAAGAGGGA CCCTGCAGAG GGGGAAACCC AGGGAAACCC AACTCGAAGC CGGCGAACTA 
     4261 AGCCTAACCA GGAAGCAGCA GCCCCCAAGG TACTGTTCAC AGGAGTGGTG GATTCTCGTG 
     4321 GAGAGCGTGC AGTGCTGGCT CTAGGAGGCA GTCTGGCCAG CTCAGTAAAT GAGGCCTCCC 
     4381 ACTTGGTTAC TGATCGCATC CGCAGGACAG TCAAGTTCTT GTGTGCCGTG GGGAAGGGAA 
     4441 TTCCCATCCT GTCCCTGAAC TGGCTCTATC AGTCCCGAAA GGCTGGTTGC TTCTTGCCGC 
     4501 CTGATGACTA CTTGGTGACT GATCCTGAAC AAGAGAAGAA TTTTAGCTTC AGCCTTCGGG 
     4561 ATTCCCTGAG CCGGGCTCGG GAACGAAGAC TACTGGAGGA CTATGAGATT CATGTGACCC 
     4621 CTGGAGTGCA GCCACCTCCA CCTCAGATGG GAGAGATCAT CAGCTGCTGT GGAGGCACTG 
     4681 TCCTGCCCAG CATGCCCCAT TCCTACAAGC TTCACAGAGT TGTCATAACG TGCACTGAAG 
     4741 ACCTACCTCG ATGTGCTATT GCATCTCGCC TGGGGCTACC CCTCCTCTCT CCTGAGTTCC 
     4801 TCCTGACTGG AGTGCTGAAG CAGGAAGCCA CACCAGAGGC CTTTGTCCTC TCCAATTTGG 
     4861 AAATGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4921 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4981 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5041 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5101 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5161 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5221 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5281 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5341 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5401 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5461 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5521 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5581 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5641 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5701 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5761 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5821 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5881 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5941 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6001 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6061 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6121 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6181 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6241 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6301 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6361 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6421 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6481 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6541 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6601 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6661 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6721 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6781 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6841 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6901 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6961 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7021 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7081 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7141 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7201 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7261 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7321 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7381 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7441 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7501 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7561 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7621 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7681 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7741 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7801 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7861 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7921 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7981 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8041 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8101 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8161 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8221 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8281 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8341 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8401 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8461 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8521 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8581 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8641 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8701 AAATTTAACG CGAATTTT
//