LOCUS       RR217572                8715 bp    DNA     circular  SYN 
DEFINITION  Npc1 (myc-DDK-tagged) - Rat Niemann-Pick disease, type C1 (Npc1)
ACCESSION   NM_153624
VERSION     NM_153624.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4872..4963 
                     /label="Myc-DDK"  
     misc_feature    4993..5578 
                     /label="PolyA signal"  
     misc_feature    5050..5069 
                     /label="Restriction Enzyme"  
     misc_feature    5854..6526 
                     /label="ColE1"  
     misc_feature    6956..7750 
                     /label="Kan/Neo"  
     misc_feature    7785..8087 
                     /label="SV40 ori"  
     misc_feature    8173..8650 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4854..4860 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4854 
                     /label="Npc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGCGCAC CACCCGGCCC TCGGCCTCCT GCTGCTGCTG CTGCTGTGCC 
     1081 CTGCGCAGGT GTTCTCACAG TCCTGTGTCT GGTACGGAGA GTGTGGAGTC GCCTTTGGAG 
     1141 ATAAGAGGTA CAACTGTGAA TATTCGGGCC CACCGAAACC CCTCCCAAAG GACGGCTATG 
     1201 ACTTAGTGCA GGAACTCTGT CCAGGACTCT TCTTTGGCAA TGTCAGTCTT TGCTGTGACG 
     1261 TTCAGCAGCT TCAGACACTG AAGAGCAACC TGCAGCTGCC CCTGCAGTTT CTGTCCAGGT 
     1321 GTCCGTCATG TTTTTATAAC CTGATGACCC TGTTTTGTGA GCTAACATGT AGCCCGCACC 
     1381 AGAGTCAGTT TCTGAATGTG ACAGCAACTG AAGATTATTT CGACCCCGAG ACTCGGGAAA 
     1441 ATAAAACAAA TGTAAAGGAA TTAGAGTACT ACGTCGGACG GAGCTTTGCG AATGCAATGT 
     1501 ACAATGCCTG CCGAGATGTG GAGGCGCCTT CCAGTAATGA GAAGGCCTTA GGACTCCTGT 
     1561 GCGGAAGGGA TGCCCGTGCC TGCAATGCCA CCAACTGGAT TGAGTACATG TTCAACAAAG 
     1621 ACAACGGGCA AGCACCGTTT ACCATCATCC CCGTATTTTC AGATCTTTCG GTTCTTGGAA 
     1681 TGGAACCCAT GAGGAATGCC ACCAAAGGCT GCAACGAGTC TGTAGATGAG GTCACAGGGC 
     1741 CGTGTAGCTG CCAGGACTGC TCTGCCGTCT GCGGCCCCAA ACCCCAGCCC CCACCGCCCC 
     1801 CTGTGCCCTG GAGGATCTTA GGTTTGGACG CCATGTATGT CATCATGTGG GTCGCCTACA 
     1861 TGGCGTTCCT GGTCCTGTTC TTTGGAGGAC TGCTGGCAGT GTGGTGCCAC AGAAGGCGGT 
     1921 ACTTTGTGTC CGAGTACACC CCCATTGACA GCAACATTGC CTTTTCCTCG AATAGCAGTG 
     1981 ACAAAGGGGA AGCCTCGTGC TGCGACCCAC TTGGTGTAGC GTTTGATGAC TGTCTGAGGC 
     2041 GCATGTTCAC AAAGTGGGGG GCTTTCTGTG TCCGGAATCC CACCTGCATC ATTTTCTTCT 
     2101 CGTTGGTCTT CATTGCTGCG TGCTCTTCTG GCCTGGTGTT TGTCCAGGTC ACCACCAATC 
     2161 CCGTGGAGCT CTGGTCAGCC CCTCACAGTC AGGCCCGCTT GGAAAAGGAG TACTTTGACA 
     2221 AGCACTTTGG GCCTTTCTTT CGTACGGAGC AGCTTATTAT CCAGGCCCCC AACTCCAGCG 
     2281 TCCATATCTA CGAGCCGTAC CCTTCCGGAG CCGATGTGCC CTTCGGGCCT CCATTGAACA 
     2341 AAGAGATTCT GCACCAGGTT CTGGACTTAC AGATCGCCAT TGAAAGTATC ACTGCGTCCT 
     2401 ATAACAATGA AACCGTGACA CTTCAGGACA TCTGTGTGGC CCCCCTCTCT CCATACAACA 
     2461 AGAACTGCAC CATTATGAGT GTGTTAAATT ACTTCCAGAA CAGCCATGCG GTGCTGGACA 
     2521 ACCAAGTAGG CGATGACTTC TTTGTCTATG CCGATTACCA CACGCACTTT CTGTACTGTG 
     2581 TGAGGGCTCC CGCCTCCTTG AATGATACGA GTTTGCTCCA CGGCCCTTGC CTGGGCACAT 
     2641 TTGGAGGACC TGTGTTCCCG TGGCTTGTGT TGGGCGGCTA TGATGATCAG AACTACAATA 
     2701 ATGCCACCGC GCTTGTGATT ACCTTCCCTG TCAATAATTA CTATAATGAC ACAGAGAAGC 
     2761 TCCAGAGGGC CTGGGCCTGG GAGAAAGAAT TTATTAACTT TGTGAAGAAC TACAAGAATC 
     2821 CAAATCTGAC CATTTCTTTC ACTGCTGAGC GAAGCATTGA AGATGAACTC AATCGGGAAA 
     2881 GCAACAGTGA CGTATTCACC GTCCTCATCA GCTATGCCGT GATGTTTCTG TACATTTCCC 
     2941 TCGCCCTGGG GCACATCCAG AGCTGGAACA GGGTCCTGGT GGATTCTAAA ATCTCGCTGG 
     3001 GCATTGCGGG GATCCTGATA GTGCTGAGCT CGGTGACCTG CTCCCTGGGC ATCTTCAGCT 
     3061 ACATCGGAAT GCCACTGACC CTCATCGTCA TTGAGGTCAT CCCGTTCCTG GTGCTGGCTG 
     3121 TTGGGGTGGA CAACATCTTC ATTCTAGTGC AGACCTACCA GAGAGATGAG CGTCTTCAGG 
     3181 AGGAGACGTT GGATCAGCAG CTGGGCAGGA TCCTGGGGGA AGTGGCCCCA ACCATGTTCC 
     3241 TTTCGTCCTT CTCTGAGACC TCGGCATTTT TCTTTGGGGC GCTGTCCTCG ATGCCAGCTG 
     3301 TCCACACCTT CTCTCTGTTT GCGGGGATGG CCGTCCTCAT TGACTTCCTC CTTCAGATTA 
     3361 CCTGCTTTGT GAGCTTCTTG GGGTTAGATA TTAAGAGACA AGAGAAAAAC CGTCTGGACA 
     3421 TCCTGTGCTG TGTCAGAGGC CCTGACGACG GACAAGAATC CCAAGCCTCC GAAAGCTACC 
     3481 TGTTCCGCTT CTTCAAAAAC GCCTTTGCCC CATTTCTGCT GACGGACTGG CTGCGGCCAG 
     3541 TTGTGATGGC CGTGTTTGTG GGCATTCTGT CATTCAGTAT TGCAGTAGTG AACAAAGTAG 
     3601 AAATCGGATT GGATCAGTCT CTCTCGATGC CAAATGATTC GTATGTGATT GACTATTTCA 
     3661 AGTCACTCGG TCAGTACCTG CACTCGGGCC CACCCGTGTA CTTCGTCCTG GAAGAGGGCT 
     3721 ACAACTACAG TTCCCGCAGA GGGCAGAACA TGGTGTGCGG TGGCATGGGC TGTGACAATG 
     3781 ACTCCCTGGT GCAGCAGATA TTTAACGCAG CAGAGCTGGA CACCTACACC CGCGTAGGCT 
     3841 TCGCTCCCTC ATCCTGGATT GATGATTACT TTGACTGGGT CTCACCGCAA TCCTCCTGCT 
     3901 GTAGACTCTA CAATGTCACT CACCAGTTCT GCAATGCTTC TGTGATTGAC CCAACCTGTG 
     3961 TCCGCTGCAG ACCTCTGACT CCAGAGGGCA AACAGAGGCC TCAGGGGAAA GAATTCATGA 
     4021 AATTCCTGCC CATGTTCCTG TCTGATAACC CCAACCCCAA GTGTGGCAAA GGGGGACATG 
     4081 CTGCTTACAG TTCTGCTGTT AACATCATGG GAGATGACAC TTACATTGGG GCCACTTACT 
     4141 TCATGACCTA CCACACCATT CTTAAGACCT CTGCTGATTA TATCGATGCC CTGAAGAAAG 
     4201 CTCGGCTTAT AGCCAGTAAC ATCACGGAAA CCATGCGTTC TAAGGGGAGT GATTATCGTG 
     4261 TGTTTCCTTA CAGTGTGTTC TATGTCTTCT ATGAACAATA CCTGACCATT ATTGATGACG 
     4321 CCATCTTTAA CCTAAGTGTG TCTCTGGGCT CCATATTTCT CGTGACCTTG GTGGCTCTGG 
     4381 GCTGTGAACT GTGGTCTGCG GTCATCATGT GTCTCACCAT AGCTATGATC CTGGTCAACA 
     4441 TGTTTGGTGT TATGTGGCTG TGGGGCATCA GTCTGAACGC TGTTTCCTTG GTCAACTTGG 
     4501 TGATGACCTG TGGCATTTCT GTGGAGTTCT GCAGCCATAT AACAAGAGCT TTCACCATGA 
     4561 GCACCAAAGG AAGCAGAGTG AGCAGGGCGG AAGAGGCACT GGCCCACATG GGTAGTTCTG 
     4621 TATTCAGTGG AATCACACTT ACAAAATTTG GAGGGATCGT GGTGTTGGCC TTTGCCAAGT 
     4681 CTCAAATTTT TGAGATCTTT TACTTCAGGA TGTACTTAGC CATGGTCTTA CTTGGAGCTA 
     4741 CTCACGGACT AATATTTCTT CCCGTCTTAC TCAGTTACAT AGGGCCATCA GTGAATAAAG 
     4801 CTAAAAGACA CACCACTCAA GAGCGGTACA AAGGGACAGA GAGAGAACGG CTCCTCAATT 
     4861 TTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4921 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4981 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5041 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5101 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5161 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5221 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5281 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5341 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5401 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5461 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5521 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5581 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5641 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5701 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5761 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5821 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5881 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5941 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6001 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6061 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6121 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6181 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6241 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6301 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6361 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6421 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6481 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6541 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6601 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6661 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6721 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6781 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6841 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6901 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6961 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7021 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7081 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7141 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7201 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7261 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7321 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7381 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7441 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7501 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7561 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7621 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7681 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7741 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7801 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7861 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7921 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7981 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8041 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8101 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8161 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8221 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8281 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8341 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8401 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8461 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8521 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8581 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8641 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8701 TTTAACGCGA ATTTT
//