LOCUS       RR217569                8676 bp    DNA     circular  SYN 
DEFINITION  Diaph1 (myc-DDK-tagged) - Rat diaphanous-related formin 1 (Diaph1)
ACCESSION   NM_001107393
VERSION     NM_001107393.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4833..4924 
                     /label="Myc-DDK"  
     misc_feature    4954..5539 
                     /label="PolyA signal"  
     misc_feature    5011..5030 
                     /label="Restriction Enzyme"  
     misc_feature    5815..6487 
                     /label="ColE1"  
     misc_feature    6917..7711 
                     /label="Kan/Neo"  
     misc_feature    7746..8048 
                     /label="SV40 ori"  
     misc_feature    8134..8611 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4815..4821 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4815 
                     /label="Diaph1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGTCC GGCGGGGGCC TAGGGCCCGG CCGCGGGACC CGGGACAAGA 
     1081 AGAAGGGTCG GAGCCCGGAT GAGCTGCCTG CGACGGGCGG CGACGGCGGC AAACATAAGA 
     1141 AATTTACTTT GAAGCGGCTC ATGGCAGACG AGCTGGAGAG ATTTACCAGC ATGAGGATTA 
     1201 AGAAGGAGAA GGAAAAGCCC AATTCTGCTC ATAGAAACTC CTCTGCATCG TATGGAGATG 
     1261 ACCCCACTGC TCAGTCACTG CAGGACATCT CAGATGATCA AGTTCTTGTC CTCTTTGAGC 
     1321 AGATGCTGGT GGATATGAAC CTGAATGAGG AGAAACAGCA GCCTTTGCGA GAGAAGGACA 
     1381 TTGTCATCAA GAGGGAGATG GTGTCCCAGT ATCTGCATAC TTCCAAAGCT GGCATGAACC 
     1441 AGAAGGAGAG TTCCAGGTCT GCCATGATGT ACATTCAGGA GCTGAGGTCA GGCTTGCGGG 
     1501 ATATGCACCT GCTTAGCTGC CTGGAGTCCC TTCGAGTCTC TCTCAACAAT AACCCTGTCA 
     1561 GTTGGGTGCA GACATTTGGT GCTGAGGGCC TAGCCTCCTT ATTGGACATC CTCAAACGAC 
     1621 TCCATGATGA GAAAGAGGAG ACTTCTGGAA ACTACGACAG CCGGAACCAG CATGAGATTA 
     1681 TCCGCTGTTT GAAAGCTTTC ATGAACAACA AGTTTGGAAT CAAAACCATG TTGGAGACGG 
     1741 AAGAAGGAAT CCTACTGCTG GTCAGAGCCA TGGATCCTGC TGTTCCCAAT ATGATGATTG 
     1801 ATGCAGCAAA GCTGCTGTCT GCCCTCTGTA TCCTACCGCA GCCAGAGGAC ATGAATGAAC 
     1861 GAGTTTTAGA GGCTATGACA GAGAGAGCTG AAATGGAAGA GGTTGAACGC TTCCAGCCAC 
     1921 TGCTGGATGG GTTAAAAAGT GGGACGTCTA TTGCACTCAA AGTGGGATGC CTACAGCTGA 
     1981 TCAATGCTCT CATCACTCCC GCTGAGGAAC TTGACTTCCG GGTTCACATC CGAAGTGAGC 
     2041 TGATGCGCTT GGGACTGCAT CAAGTGTTGC AGGAGCTTCG AGAGATTGAC AATGATGATA 
     2101 TGCGAGTACA GCTCAATGTG TTTGATGAAC AAGGGGATGA AGATTTCTTC GATCTGAAGG 
     2161 GACGACTGGA TGATATCCGC ATGGAGATGG ATGACTTTGG TGAAGTTTTC CAGATTATTT 
     2221 TAAACACAGT GAAAGATTCC AAGGCAGAAC CACACTTCCT GTCTATCTTG CAGCATCTCC 
     2281 TGTTGGTCCG AAATGATTAT GAAGCTAGGC CGCAGTACTA TAAATTGATT GAAGAATGTG 
     2341 TTTCTCAGAT AGTTCTACAC AAAAATGGAA CTGATCCTGA CTTCAAGTGC CGGCACCTGC 
     2401 AGATTGATAT TGAGGGGTTG GTTGATCAAA TGATTGATAA AACGAAGGTG GAAAAATCTG 
     2461 AGGCCAAAGC TACAGAGCTG GAAAAAAAGT TGGACTCAGA ATTAACAGCC CGGCATGAGT 
     2521 TACAAGTAGA AATGAAAAAG ATGGAAAATG ACTTTGAGCA GAAACTTCAG GATCTTCAAG 
     2581 GAGAAAAGGA TGCCTTGGAT TCTGAAAAGC AGCAGATCAC TACACAAAAA CAAGACCTGG 
     2641 AAGCAGAGGT GTCCAAGCTG ACAGGAGAGG TTGCCAAGCT GTCAAAAGAA CTAGAAGATG 
     2701 CCAAGAAGGA AATGGCTTCT CTCTCTGCTG TGGCTGTTGC ACCTTCAGTT TCTAGTAGTG 
     2761 CTGCTGTTCC CCAAGCCCCT CCTCTGCCTG GCACTTCAGG CACTGTTATT CCACCTCCCC 
     2821 CACCACCCCC TCTCCCTGGA GGTGCCGTCC CACCCCCTCC TCCTCCTCCT CTGCCTGCAG 
     2881 GTACTGGTAT CCCTCCACCT CCTCCTTTAC CTGGAGGTGC TTGTATATCC TCTTCCCCAC 
     2941 AGTTGTTTGG CAGTACTGCC ATTCCCCCAC CTCCTCCTTT GCCTGGAGCT ACTGCCATCC 
     3001 CCCCACCTCC CCCTTTGCCT GGAGATACTG CCATCCCCCC ACCTCCCCCT TTGCCTGGAG 
     3061 NTACTGCCAT CCCCCCTCCT CCTCCTTTGC CTGGAGCTAC TGGTGTACCA CCACCTCCTC 
     3121 CCCCTTTACC TGGAAGTGTT GGTGTTCCCC CACCCCCTCC CTTGCCTGGA GGACCAGGAC 
     3181 TGCCTCCTCC TCCTCCCCCC TTTCCTGGAG CACCTGGCAT TCCCCCCCCA CCGCCTGGTA 
     3241 TGGGCGTGCC TCCACCTCCC CCCTTTGGAT TTGGGATTCC TGCAGCCCCA GTTCTGCCAT 
     3301 TTGGATTAAC CCCCAAAAAA GTTTATAAGC CAGAGGTGCA ACTCCGGAGG CCAAACTGGT 
     3361 CCAAGTTCGT GGCTGAGGAC CTTTCCCAGG ACTGCTTCTG GACAAAGGTG AAGGAGGACC 
     3421 GCTTTGAGAA CAATGAACTA TTTGCCAAAC TTACCCTTGC CTTCTCTGCC CAGACCAAGA 
     3481 CTTCTAAAGC CAAGAAGGAT CAAGAAGGTG GAGAAGAAAA GAAATCTGTT CAAAAGAAGA 
     3541 AAGTAAAAGA GCTGAAAGTG CTGGATTCAA AGACAGCCCA GAATCTCTCA ATCTTTTTGG 
     3601 GTTCATTCCG TATGCCCTAT CAAGAGATTA AGAACGTTAT CCTGGAGGTG AATGAGGCTG 
     3661 TTCTCACGGA GTCTATGATC CAGAACCTCA TTAAGCAGAT GCCAGAACCA GAGCAGTTAA 
     3721 AAATGCTTTC TGAACTGAAG GAGGAGTACG ATGATCTGGC TGAGTCAGAG CAGTTCGGCG 
     3781 TGGTGATGGG CACGGTTCCC CGTCTTCGGC CTCGCCTCAA TGCCATCCTT TTCAAGCTAC 
     3841 AGTTCAGTGA GCAAGTTGAG AATATCAAGC CAGAGATTGT ATCTGTCACT GCGGCATGTG 
     3901 AGGAGCTGCG TAAGAGTGAG AACTTCTCTA GCCTCCTGGA GCTCACACTG CTCGTTGGAA 
     3961 ACTATATGAA TGCGGGCTCC AGGAATGCTG GAGCTTTTGG CTTCAATATC AGCTTCCTTT 
     4021 GTAAGCTTCG AGACACCAAG TCTGCAGATC AGAAGATGAC TCTGTTGCAT TTCTTGGCTG 
     4081 AGTTATGTGA GACTGACCAC CCCGATGTCC TCAAGTTTCC TGATGAGCTT GCCCATGTAG 
     4141 AGAAAGCCAG CAGAGTTTCT GCTGAGAACT TGCAGAAGAA TTTAGATCAG ATGAAGAAAC 
     4201 AGATTGCTGA TGTGGAACGC GATGTTCAAA ATTTCCCAGC TGCCACAGAC GAAAAAGACA 
     4261 AGTTTGTTGA GAAGATGACT AGCTTTGTGA AGGATGCACA GGAACAGTAT AACAAGCTAC 
     4321 GGATGATGCA CTCCAACATG GAGACTCTCT ATAAGGAGCT AGGCGACTAC TTCGTCTTTG 
     4381 ACCCTAAGAA GTTGTCTGTA GAAGAATTTT TTATGGATCT GCACAACTTT AGGAATATGT 
     4441 TTTTGCAAGC AGTCAAGGAG AACCAGAAGC GCCGGGAAAC AGAAGAAAAG ATGAGGAGAG 
     4501 CAAAACTAGC CAAGGAAAAG GCGGAAAAAG AGCGACTGGA GAAGCAGCAG AAGCGAGAGC 
     4561 AGCTCATAGA CATGAATGCA GAGGGGGATG AGACAGGTGT GATGGACAGT CTTCTAGAAG 
     4621 CTCTGCAGTC AGGAGCAGCA TTCCGACGGA AGAGAGGGCC CCGGCAGGTC AACAGGAAAG 
     4681 CTGGGTGTGC AGTCACATCT CTGCTAGCCT CGGAGCTGAC CAAGGATGAT GCCGTGGCTG 
     4741 CTAGTTCTGC TAAGGTGCCC AAGAAAAGTG AAGGAGTCAC CACAATCCTT GAAGAAGCCA 
     4801 AGGAACTGGT TGGCCGTGCA AGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4861 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4921 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4981 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5041 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5101 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5161 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5221 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5281 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5341 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5401 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5461 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5521 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5581 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5641 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5701 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5761 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5821 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5881 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5941 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6001 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6061 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6121 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6181 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6241 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6301 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6361 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6421 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6481 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6541 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6601 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6661 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6721 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6781 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6841 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6901 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6961 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7021 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7081 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7141 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7201 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7261 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7321 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7381 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7441 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7501 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7561 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7621 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7681 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7741 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7801 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7861 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7921 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8101 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8161 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8221 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8281 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8341 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8401 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8461 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8521 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8581 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8641 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//