LOCUS       RR217568                8676 bp    DNA     circular  SYN 
DEFINITION  Synpo2 (myc-DDK-tagged) - Rat synaptopodin 2 (Synpo2)
ACCESSION   NM_001191963
VERSION     NM_001191963.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4831..4922 
                     /label="Myc-DDK"  
     misc_feature    4952..5537 
                     /label="PolyA signal"  
     misc_feature    5009..5028 
                     /label="Restriction Enzyme"  
     misc_feature    5813..6485 
                     /label="ColE1"  
     misc_feature    6915..7709 
                     /label="Kan/Neo"  
     misc_feature    7744..8046 
                     /label="SV40 ori"  
     misc_feature    8132..8609 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4806..4813 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4806 
                     /label="Synpo2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCACCGGA GATTTCATCT GCATTTCCAT GACTGGAGGG GCGCCCTGGG 
     1081 GGTTCAGGCT GCAAGGAGGC AAGGAGGAGA AGCAGCCCTT GCAAGTGGCA AAGATTCGAA 
     1141 GTCAGAGCAA GGCATCGGAC TCCGGGCTGT GTGTTGGGGA TGAAGTGGTG TCCATCAATG 
     1201 GTAACCCTTG TGCAGACCTT ACTTACCCTG AAGTCATCAA ACTCATGGAA AGCATAACGG 
     1261 ACTCCCTCCA TCTGCTTATT AAAAGACCCA CCAGTGGAAC AAGTGAGGCT TTGGATTCTG 
     1321 AAACAGAAAA TACAAACCAC CAGCACCTTC CACACGGGGG GCCCATGGAA AGTACCACCC 
     1381 TGCAGATCCA GCACGCTGCC AAGACCCAGG GCAAAGACTT CCTTCTTGCT TCAGTCCAGA 
     1441 CTAGTGCTCC CCGAACTGAG GACCAAGGAA ATGCCTGGGG TTATGCAGAG TGTACAACAG 
     1501 AAGACCAAGT TTCCCAGATG CCTGGCTCCC AAGAAGGACA CTTGGTAGAA GAGGTCATCT 
     1561 TAAGGAAAAA GCCAGAAGCA GGCCAGCCAG GCCATGTGGT TGAGTTACAG CTGTCCCTCT 
     1621 CAAAGGAAAG ACAGCGATGC ACCAGTGACC CTATAGTGAC TCTCCTGGGA AATGAAAAAT 
     1681 TCAAGTCTCC AGACCCAGAC TGGGGCACCC AACACGGGAG GACTGTCCAC ATAAATTCAA 
     1741 TCCCTGCTCC TGAGAAAGCA GACACTTCTC TGACGTCCGG CACCACAGTC CAGACCTCCA 
     1801 GTGGCCGGGA GCTGACAGTG ATCCAGGGAA GAGACCCAGG AGGCACAGGG CTGCCCCAGG 
     1861 TGGAAGTGAT TCTAGATTGC TCTGACAGGC TGAAGGCTGA AGAGTGCAGG CTGCAGGCAG 
     1921 GAAGGGGGTG TGTGGCTTCT CCCGTGGAAG GAGGGCGGTC AGAAGCACCT CCTTCTCTGG 
     1981 TCTCCTTTGC GGTCTCATCA GAAGGCACCG AGCAGGGAGA AGATCAACGC TCGGGAAAGG 
     2041 ACCAGGGCAG ACCACACAAG CACAGAGCGA GGCATGCCCG GCTCAGGAGG AGTGAAAGTC 
     2101 TGTCTGAAAA GCAAGTAAAA GAAGCCAAGT CTAAATGCAA AAGCATCGCC CTCCTCCTAA 
     2161 CGGATGCTCC CAACCCGAAC TCCAAGGGGG TGTTGATGTT TAAGAAGCGG AGGCGGAGAG 
     2221 CCCGGAAGTA CACCCTAGTC AGTTACGGTA CTGGAGAGCT TGAGCGAGAG GAGGAAGAGG 
     2281 AGGACCAGGA AGCAGGTGAC AAGGACGAGA TAAGTGACCT TGCATTTCTT GGAACCAGCG 
     2341 AGTCGGAAGT GGATGAAGAG TTACTGTCTG ACGTGGACGA CAACACCCAA GTTGTGAACT 
     2401 TCGATTGGGA TTCTGGACTG GTGGACATTG AAAAGAGGCT GAACCGAGGC GACAAGATGG 
     2461 AGATGTTGCC CGACACCACA GGCAAGGGAG CGCTCATGTT TGCCAAGAGG AGAGAGAGGA 
     2521 TGGAACAGTT CACAGCCCAA AATGAAGAGG AAAAGACCGG TGGGCTAGCA GGCGGAGGAT 
     2581 CAGATGCCCT ACAGACGGAC GGTCTGAGAA CCATGACTTC TTATCAAAGA AAAGAAGAAT 
     2641 CGGTGAGAAT GCAGAGCTCT GTGAGTGAGA GCTCCTTCCA GATGGGACGC AGTCTTGGCA 
     2701 GTGTGCCACA ACAGAATGGC TTCAGTGGAG TGTCTGAGAC AGCAGGGCCC CAGAGGATGA 
     2761 TCCCCATGAA CAGAACCGCC AAGCCCTTCC TGGGATCTGT GAACCAGACA GCGGCCCCAT 
     2821 TCTCCCCAAC ACAGAGTGTG ACAAGTCCCA TCCCTGACTT CCCTGCGCCA CCACCATACT 
     2881 CTGCAGTCTC ACCTCCACCA GAAGCCTTCT CCAGGGGGAT ATCAAGCCCA GTTGCTGGCC 
     2941 CGGCACAGCC CCCTCCGTGG CCCCAGCCTG CTCCATGGTC GCAACCAGCC TTCTATGATT 
     3001 CTTCTGAGCA AATAGCTTCC CGCGATGAGA GGATCGCAGT GCCGGCCAAA AGAACAGGAA 
     3061 TACTGCAGGA AGCCAAAAGA CGAGGCACAA CAAAACCCAT GTTTACTTTC AAAGAAACCA 
     3121 AAGTGAGCCC CAACCCCGAA CTCCTGTCCC TTCTTCAAAA TGCAGAAGGC AAGCGAGGCA 
     3181 CTGGAGCTGG AGGGGACTCC GGGCCAGAAG AAGACTACCT CAGTTTAGGC GCAGAGGCTT 
     3241 GTAATTTCAT GCAGAGTTCT GCCAAACAAA AGACCCCTCC TCCCGTCGCT CCAAAGCCTG 
     3301 CAGTCAAGAC CTCCTCTTCC TCCCAACCAG TAGCCCCTGT TTCTCCAGTC TGGTCTCCAG 
     3361 GAGTGGCTCC AGCCCAGGGT CCTGCCTTCT CTACAACAAA CCCACCAAAC CCACCACAGG 
     3421 TCACTGCTGT GTCCTCCATC AAAATAGCCC AGCCTACCTG CCCTCCAGCC CGGCCCGCGA 
     3481 GTGCACTGAA CCTGGCTGGT CCTTTCAAAG GTCCCCAAGC AGCAGTAGTC AGTCATAACT 
     3541 ATACACCCAA GCCATCAGCA CCCACACCAT TAGTAAATGC TGCTCCTGCT GGTGCCGGGG 
     3601 GACCATCCAA TGAGCTTCCT GGAATGAGTG GGAAAGGAGC CCAACTCTTC GCTAAAAGGC 
     3661 AGTCTAGGAT GGAGAAGTAT GTGGTAGATT CTGACACGGT GCAGGCCCAC ACAGTTCGGG 
     3721 CCCAGTCTCC TACTCCATCC CTGCCCGCCA GTTGGAAGTA CTCCTCCAAT GTCCGAGCAC 
     3781 CGCCTCCGGT GGCCTACAAT CCTATCCATT CCCCGTCTTA CCCACTGGCT GCTATCAAGT 
     3841 CTCAGCCACC GGGAGCCCAG GCCTCCAAAA CGAGCAAGAA AAAGGGCAAG AAACCTCTTA 
     3901 ACACTTTGGA TGTCATGAAG CACCAACCAT ATCAGCTCAA CGCGTCTTTG TTTACTTTTC 
     3961 AACCCCCCGA TTCAAAGGAT GGCCTCCCTC AAAAGTCAAC AGTCAAGGTC AGTTCAGTAG 
     4021 CCCCTGCCAT GAAGCAAGCC CTTCCTCCCC GGCAGGCGGA TATTGGCTCT CCCACAAACG 
     4081 CGAAGGCTTC ATCTGTGTAT TCAGTCCCGG CCTATACCTC TCAGCCCAAC TTCTTTGCGG 
     4141 AGGCCACCTC ACCAGTCAGC GCATCCCCGG TGCCTGTGAG TGTTCCCACC TCTCCAAAGC 
     4201 AAGAAACGAC CTCCACATCC TATTTTGTGG CTCCGAGGCC GAAGTTCTCA GCCAAGAAAA 
     4261 GTGGTGTCAC AGTTCAGGAG AACTGGCGCT CCCTTTCTCT CCCCGGAAGA GCAGCTCCGC 
     4321 CCATCATGTC TGCACCTCCC TGGCTGTGCC AGCCTGCCTA CAGTTACTCC AGCAAACCAA 
     4381 CCCTCGAGCA AGAGAAAGCT AACAAGAGAC CTACACCTTG GGAGGCGGCG GCCAAGTCCC 
     4441 CTCTCGGTCT AGTGGATGAG GCCTTCAGAC CCAGGAACAT CGAGGAATCC ATTGTGGCGA 
     4501 ATGTAGTCTC TGCAGCTCGG AGGAAGGTGT TTGCAGGGTC CCAAGAGGAC TGGAAAGAGA 
     4561 GACTGTCCTT TGTCCCTCAA ACTCAGAAGA CCAGCATGAG CTTTTCTGAA AGGCGAGAGT 
     4621 ATAATGTTCC ATCCCCAGTC AACAGCCACG TGTCTAGCCA CTCCTTATAT AGCTCCCAGT 
     4681 TGCCATATGT ATGCTACAGG AAGGAGTCCA GAAATGATTT GAAAGCAATG TCCATGGACA 
     4741 CGAGGTCTGA ATACTGTCTT CCACTAGGTG GTTATGACTA TAACCCACAC CCAAGGGGGT 
     4801 GGAGACACCA ACCAAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4861 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4921 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4981 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5041 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5101 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5161 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5221 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5281 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5341 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5401 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5461 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5521 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5581 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5641 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5701 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5761 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5821 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5881 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5941 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6001 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6061 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6121 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6181 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6241 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6301 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6361 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6421 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6481 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6541 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6601 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6661 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6721 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6781 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6841 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6901 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6961 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7021 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7081 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7141 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7201 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7261 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7321 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7381 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7441 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7501 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7561 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7621 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7681 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7741 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7801 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7861 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7921 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8101 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8161 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8221 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8281 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8341 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8401 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8461 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8521 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8581 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8641 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//