LOCUS       RR217561                8613 bp    DNA     circular  SYN 
DEFINITION  Rasgrf1 (myc-DDK-tagged) - Rat RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1
ACCESSION   NM_001170531
VERSION     NM_001170531.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4770..4861 
                     /label="Myc-DDK"  
     misc_feature    4891..5476 
                     /label="PolyA signal"  
     misc_feature    4948..4967 
                     /label="Restriction Enzyme"  
     misc_feature    5752..6424 
                     /label="ColE1"  
     misc_feature    6854..7648 
                     /label="Kan/Neo"  
     misc_feature    7683..7985 
                     /label="SV40 ori"  
     misc_feature    8071..8548 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4752..4758 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4752 
                     /label="Rasgrf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAAAGCC ATCCGACTGA ACGATGGCCA CGTCGTGTCC CTGGGACTGC 
     1081 TGGCCCAGAG AGACGGCACG CGCAAAGGCT ACCTGAGCAA GAGGAGTTCG GACAACCCAA 
     1141 AATGGCAAAC CAAGTGGTTT GCGCTGCTGC AGAACCTGCT CTTCTACTTC GAAAGTGACT 
     1201 CGAGCTCTCG GCCCTCGGGG CTCTACCTGC TGGAGGGCAG TATCTGCAAA CGCATGCCCT 
     1261 CCCCCAAGCG AGGGACCTCC TCCAAGGAGT CCGACAAACA GCATCATTAC TTCACAGTGA 
     1321 ACTTCTCCAA TGACAGCCAG AAGTCCCTAG AGCTGAGGAC CGATGACTCC AAGGACTGTG 
     1381 ACGAGTGGGT GGCAGCGATT GCTCGCGCCA GCTACAAGAT ACTGGCCACA GAGCATGAGG 
     1441 CGCTCATGCA GAAGTACCTG CACCTGCTGC AGGTGGTGGA GACAGAGAAG ACCGTGGCTA 
     1501 AGCAGCTGCG ACAGCAGCTC GAGGATGGCG AGGTCGAGAT CGAGCGCCTG AAGGCAGAGA 
     1561 TTGCAAACCT GATCAAGGAC AATGAACGTA TCCAGTCCAA CCAGCTGGTT GCCCCTGAGG 
     1621 ATGAGGACAG TGACATCAAG AAAATTAAGA AGGTACAGAG TTTCCTTCGC GGATGGCTGT 
     1681 GCCGGCGAAA GTGGAAGAAC ATCATCCAGG ACTACATCCG GTCTCCTCAT GCCGACAGCA 
     1741 TGCGCAAGAG GAACCAGGTG GTGTTCAGCA TGCTGGAAGC TGAGGCCGAG TACGTGCAGC 
     1801 AACTACACAT CCTTGTCAAC AATTTTCTGC GCCCACTGCG CATGGCCGCC AGCTCTAAGA 
     1861 AACCCCCTAT AACACATGAC GACGTCAGCA GTATCTTTCT GAACAGTGAG ACCATCATGT 
     1921 TCCTGCACCA GATCTTCTAC CAAGGCCTGA AGGCCCGTAT CGCCAGCTGG CCCACCCTGG 
     1981 TTCTGGCGGA CCTGTTCGAC ATCCTGCTGC CAATGCTTAA CATCTACCAG GAGTTCGTCC 
     2041 GCAACCACCA GTACAGTCTC CAGATCCTAG CACACTGCAA GCAAAACCGG GACTTTGACA 
     2101 AGCTCCTCAA GCAGTATGAG GCCAAGCCAG ACTGCGAGGA GCGCACACTG GAGACCTTCC 
     2161 TCACCTATCC AATGTTCCAG ATCCCCAGGT ACATCCTGAC ACTCCATGAG CTGCTGGCCC 
     2221 ACACACCTCA TGAGCATGTG GAGCGCAACA GCCTGGACTA TGCCAAATCC AAACTAGAGG 
     2281 AGCTGTCCAG GGTCATGCAC GACGAAGTCA GTGAGACCGA GAACATCCGC AAAAACCTGG 
     2341 CCATTGAGCG TATGATCACC GAGGGCTGTG AGATCCTCCT TGACACCAGC CAGACCTTTG 
     2401 TGCGCCAAGG TTCCCTCATC CAGGTGCCCA TGTCAGAAAA GGGCAAGATC AACAAGGGCC 
     2461 GCCTGGGGTC TCTGTCCCTT AAGAAAGAAG GTGAGCGCCA GTGTTTCCTG TTCTCCAAGC 
     2521 ATCTCATCAT CTGCACCAGA GGCTCTGGTA GCAAACTGCA CCTAACCAAG AATGGCGTGA 
     2581 TTTCCCTCAT TGACTGCACT CTACTGGATG ATCCAGAAAA CATGGATGAT GACGGCAAAG 
     2641 GACAAGAGGT AGATCACCTG GACTTTAAGA TTTGGGTGGA GCCAAAGGAT TCCCCACCCT 
     2701 TCACAGTCAT CCTGGTGGCC TCATCCAGGC AGGAGAAGGC GGCATGGACC AGTGACATCA 
     2761 TCCAGTGCGT GGATAATATC CGCTGCAACG GGCTCATGAT GAATGCCTTT GAAGAAAATT 
     2821 CCAAGGTCAC CGTGCCGCAG ATGATCAAGT CTGATGCTTC CTTATACTGT GATGATGTTG 
     2881 ACATTCGCTT CAGCAAAACC ATGAATTCTT GCAAAGTGCT GCAGATCCGC TATGCCAGCG 
     2941 TGGAGCGCCT GCTGGAGCGC CTGACTGATC TTCGCTTCCT GAGTATTGAC TTTCTCAACA 
     3001 CCTTCCTGCA CTCCTATCGA GTCTTCACCG ATGCTGTGGT GGTCCTAGAC AAGCTGATCA 
     3061 GCATCTACAA AAAGCCCATC ACTGCGATTC CTGCCAGGTC ACTGGAACTC CTGTTCTCCA 
     3121 GTAGCCACAA CACCAAACTT CTGTACGGAG ATGCCCCCAA GTCGCCTCGT GCCAGCCGCA 
     3181 AGTTCTCCTC GCCGCCGCCC TTGGCCATCG GCACTTCGTC CCCAGTCCGC CGCCGGAAGT 
     3241 TGTCTCTCAA CATTCCCATC ATCACAGGCG GCAAGGCGCT GGAACTGGCT TCGCTCGGGT 
     3301 GCCCCTCCGA CGGCTACACC AACATACACT CGCCCATATC TCCCTTCGGC AAAACCACGC 
     3361 TGGACACCAG CAAGCTCTGT GTGGCCAGCA GCTTGACCAG AACGCCGGAG GAGATTGATA 
     3421 TGACCACTCT AGAGGAGTCA TCAGGCTTCA GGAAGCCGAC CTCAGACATC TTGAAAGAAG 
     3481 AGTCTGATGA TGACCAGAGT GATGTAGACG ACACAGAAGT GTCTCCACCA ACACCGAAAT 
     3541 CATTCAGAAA CAGAATCACT CAAGAGTTCC CACTCTTTAA CTACAACAGT GGAATCATGA 
     3601 TGACATGTCG CGATCTGATG GACAGTAACC GCAGCCCTCT GTCAGCTACC TCTGCCTTTG 
     3661 CCATAGCGAC TGCAGGAGCC AATGAAAGCC CCGCAAACAA GGAGATATAT CGAAGGATGT 
     3721 CTTTGGCCAA CACAGGGTAT TCCTCTGACC AGAGAAATAT CGACAAAGAG TTCGTGATCC 
     3781 GCAGAGCGGC CACCAACCGT GTACTGAATG TGTTGCGCCA CTGGGTCACC AAGCACTCCC 
     3841 AGGACTTTGA AACTGACGAC CTCCTCAAAT ACAAGGTGAT CTGCTTTCTG GAAGAGGTCA 
     3901 TGCATGACCC AGACCTTCTA CCACAAGAGC GAAAGGCAGC AGCCAACATC ATGAGGACTC 
     3961 TGACCCAGGA AGAAATAACT GAAAACCATA GCATGCTGGA TGAGCTCTTA CTAATGACGG 
     4021 AGGGTGTGAA GACTGAGCCC TTCGAAAACC ACTCAGCCAT GGAGATAGCA GAGCAGCTGA 
     4081 CCCTGCTGGA TCACCTTGTC TTCAAGAGTA TTCCTTATGA GGAATTCTTT GGCCAGGGCT 
     4141 GGATGAAGGC AGATAAGAAT GAAAGGACAC CTTACATTAT GAAAACCACC AGACATTTCA 
     4201 ACCATATCAG TAACTTGATC GCTTCAGAAA TTCTCCGAAA CGAGGAGGTC AGTGCAAGGG 
     4261 CAAGCACCAT CGAGAAGTGG GTGGCTGTTG CCGACATTTG CCGCTGCCTG CACAACTACA 
     4321 ATGCTGTGCT GGAGATCACT TCCTCCATCA ACCGCAGCGC AATCTTCCGA CTCAAGAAGA 
     4381 CATGGCTCAA AGTTTCTAAG CAGACGAAAT CTCTGTTTGA CAAGCTCCAA AAGCTTGTGT 
     4441 CATCAGATGG CCGATTTAAG AACCTCAGAG AAACTTTGCG AAATTGTGAT CCACCCTGTG 
     4501 TCCCTTACCT GGGGATGTAC CTGACCGACT TGGCATTCCT CGAGGAAGGA ACACCCAATT 
     4561 ACACAGAGGA CGGCCTGGTC AACTTCTCCA AGATGAGGAT GATCTCCCAT ATTATCCGCG 
     4621 AGATTCGCCA GTTTCAGCAG ACTACTTACA AAATCGAGCC CCAGCCAAAG GTAACTCAGT 
     4681 ACTTAGTGGA TGAAACCTTT GTGTTGGACG ACGAAAGTCT GTATGAGGCC TCCCTCCGAA 
     4741 TTGAACCAAA ACTCCCCACA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4801 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4861 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4921 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4981 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5041 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5101 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5161 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5221 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5281 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5341 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5401 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5461 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5521 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5581 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5641 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5701 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5761 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5821 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5881 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5941 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6001 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6061 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6121 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6181 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6241 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6301 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6361 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6421 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6481 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6541 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6601 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6661 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6721 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6781 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6841 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6901 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6961 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7021 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7081 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7141 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7201 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7261 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7321 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7381 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7441 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7501 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7561 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7621 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7681 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7741 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7801 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7861 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8041 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8101 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8161 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8221 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8281 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8341 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8401 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8461 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8521 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8581 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//