LOCUS       RR217559                8601 bp    DNA     circular  SYN 
DEFINITION  Nfasc (myc-DDK-tagged) - Rat neurofascin (Nfasc), transcript variant 1
ACCESSION   NM_001160314
VERSION     NM_001160314.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4758..4849 
                     /label="Myc-DDK"  
     misc_feature    4879..5464 
                     /label="PolyA signal"  
     misc_feature    4936..4955 
                     /label="Restriction Enzyme"  
     misc_feature    5740..6412 
                     /label="ColE1"  
     misc_feature    6842..7636 
                     /label="Kan/Neo"  
     misc_feature    7671..7973 
                     /label="SV40 ori"  
     misc_feature    8059..8536 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4740..4746 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4740 
                     /label="Nfasc"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGGCAG CAGGCGCCAC CCTGGGTCCA CGTAGCCCTC ATCCTCTTCC 
     1081 TCCTCAGCCT CGGAGGGGCC ATTGAGATTC CGATGGATCC AAGCATTCAG AATGAGCTGA 
     1141 CCCAACCCCC AACGATCACC AAGCAGTCGG TGAAGGACCA CATCGTGGAC CCCCGAGATA 
     1201 ACATCCTGAT TGAATGTGAA GCTAAAGGGA ACCCCGCCCC CAGTTTCCAC TGGACTCGCA 
     1261 ACAGCAGGTT CTTCAACATT GCCAAGGACC CACGGGTGTC CATGAGGAGG AGGTCTGGGA 
     1321 CCTTGGTGAT CGACTTCCGC AGTGGTGGGC GGCCTGAGGA GTACGAAGGG GAGTACCAGT 
     1381 GCTTTGCCCG GAACAAATTC GGCACAGCTC TTAGCAACCG CATCCGCCTG CAGGTGTCCA 
     1441 AATCTCCCCT GTGGCCCAAG GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT 
     1501 TAACCCTGCA GTGCAACCCC CCACCTGGCC TCCCATCCCC CGTCATCTTC TGGATGAGCA 
     1561 GCTCCATGGA GCCCATCACC CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT 
     1621 ACTTCTCCAA CGTCATGCTG CAGGACATGC AGACCGACTA CAGCTGCAAT GCACGCTTCC 
     1681 ACTTCACCCA CACCATTCAG CAGAAGAATC CCTTCACCCT CAAAGTCCTC ACCACCCGAG 
     1741 GAGTTGCGGA AAGAACACCT AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG 
     1801 TACTGCGCGG CATGGACCTG CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA 
     1861 TTGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA 
     1921 ACAAGGCTCT GCGCATCACC AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG 
     1981 CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT 
     2041 ACTGGCTGGA TGAGCCCAAG AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT 
     2101 GTCGAGCCAA TGGGAACCCG AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC 
     2161 AATCGGCACC ACCCAACCCC AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA 
     2221 CTCAGATCAG CAGCAGGGCA GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC 
     2281 TGGCCAATGC CTTCGTCAGC GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC 
     2341 AGCTCATCAG GGTGATCCTT TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC 
     2401 CCATTCCAAC ACTCCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT 
     2461 ACCACGTCTA CGAAAATGGC AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA 
     2521 TCTACACCTG TGTGGCCACC AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG 
     2581 TCAAAGACCC CACCAGGATC TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA 
     2641 CAGTGCAGCT GGAGTGCCGT GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC 
     2701 TGAAGGATGA CGAGCCACTC TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA 
     2761 CCATCTTCGG AGTGGCAGAG CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC 
     2821 TGGACCAGGA CCTGGCAAAG GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCGACTA 
     2881 ACCGTTTGGC TGCCCTGCCC AAAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG 
     2941 ACCTGGCGGA AAGGAGTGTG AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA 
     3001 TCACAGACTA CGTTGTCCAG TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC 
     3061 ACTCCAAGTT CCCAGGCAGT GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT 
     3121 ATCAGTTCCG AGTCATCGCT GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG 
     3181 AGCGGTACCG AACAAGCGGG GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG 
     3241 GGACACGAAA AAACAATATG GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG 
     3301 GCCCCAACCT GCGCTACATT GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA 
     3361 ATGTCACCGT GTGGGGCTCT CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG 
     3421 AGATCCGAGT CCAGGCTGAA AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG 
     3481 GGTACTCGGG GGAAGATTTA CCCAGTGCCC CCAGGCGTTT CAGAGTCCGA CAGCCCAACC 
     3541 TGGAGACCAT CAACCTGGAA TGGGATCACC CAGAGCACCC CAACGGGATC CTGATTGGAT 
     3601 ACACGCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG GTGGAAAACT 
     3661 TCTCTCCCAA TCAGACCAAG TTCTCGGTGC AGAGAGCAGA CCCCGTGTCC CGTTACCGCT 
     3721 TCTCCCTCAG TGCCAGGACG CAGGTGGGCT CTGGAGAAGC AGCCACGGAG GAGTCCCCAA 
     3781 CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC ATTGCCCCCG ACTACCGTGG 
     3841 GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC AGTGAAGCCA 
     3901 CAACAGTTCC CATCATCCCA ACGGTGGTAC CTACCACCGT CGCCACCACC ATTGCCACAA 
     3961 CTACTACAAC CACTGCCGCC GCCACCACCA CTACCACCAC GGAGAGTCCT CCCACTACCA 
     4021 CCACTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG AACGTCACGG 
     4081 TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG CCTGGAACTG 
     4141 ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT GTTAAGGCCC 
     4201 AAGCCCAGCC CATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG TTGCGGGTGT 
     4261 ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC AGTACAGCTT 
     4321 ACACCAACAA CCAGACTGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGTGCCA 
     4381 TTGCCCTTCT GGTGCTGATC CTGCTGATCG TCTGCTTCAT CAAGAGGAGT CGTGGCGGCA 
     4441 AGTACCCAGT GCGAGAAAAG AAGGATGTCC CCTTGGGCCC TGAAGACCCC AAAGAAGAAG 
     4501 ATGGTTCATT TGACTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCTC 
     4561 TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT AGTGGACTAC GGCGAGGGTG 
     4621 GCGAGGGCCA GTTCAACGAA GATGGCTCCT TTATTGGCCA GTACACTGTC AGAAAGGACA 
     4681 AGGAGGAGAC CGAGGGCAAT GAGAGCTCAG AGGCCACATC TCCAGTCAAT GCCATCTATT 
     4741 CCCTGGCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4801 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4861 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4921 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4981 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5041 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5101 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5161 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5221 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5281 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5341 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5401 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5461 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5521 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5581 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5641 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5701 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5761 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5821 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5881 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5941 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6001 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6061 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6121 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6181 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6241 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6301 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6361 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6421 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6481 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6541 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6601 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6661 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6721 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6781 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6841 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6901 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6961 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7021 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7081 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7141 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7201 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7261 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7321 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7381 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7441 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7501 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7561 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7621 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7681 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7741 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7801 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7981 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8041 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8101 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8161 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8221 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8281 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8341 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8401 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8461 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8521 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8581 CAAAAATTTA ACGCGAATTT T
//