LOCUS       RR217552                8556 bp    DNA     circular  SYN 
DEFINITION  Aff3 (myc-DDK-tagged) - Rat AF4/FMR2 family, member 3 (Aff3)
ACCESSION   NM_001191887
VERSION     NM_001191887.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4713..4804 
                     /label="Myc-DDK"  
     misc_feature    4834..5419 
                     /label="PolyA signal"  
     misc_feature    4891..4910 
                     /label="Restriction Enzyme"  
     misc_feature    5695..6367 
                     /label="ColE1"  
     misc_feature    6797..7591 
                     /label="Kan/Neo"  
     misc_feature    7626..7928 
                     /label="SV40 ori"  
     misc_feature    8014..8491 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4695..4701 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4695 
                     /label="Aff3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCACTCTA GGCCGTGTCT ATGAACCAGA TAGGAATGCG TTACGGAGGA 
     1081 AAGAACGGGA AAGGAGAAGT CAGGAAACGC AGCAGGACAG CGGCGGCTTT AACTCCGGTT 
     1141 ACTCACTCTT CAGTGAACCC TACAAGACTA ACAAGGGGGA TGAACTCTCC AACCGGATCC 
     1201 AGAATACATT AGGAAATTAT GACGAGATGA AAGACTTTTT AACTGACCGA TCCAACCAGA 
     1261 GTCATCTTGT TGGCGTCCCC AAGCCTGGTG TTCCCCAGAC TCCTGTGAAC AAGATCGATG 
     1321 ACCATTTTGT GGCAGAGTCA AGAGCCCAGC CCCAGCCCTC ATCTGTCTGC AGCACTGCAT 
     1381 CTTCCACACC TGCAGCTGTC CCAGTGCAGC AGAGCAAGAG AGGCACCATG GGCTGGCAGA 
     1441 AGGCTGGGCA CCCACCGTCT GACGGCCAAC AGAGAGCAGC GCAACAGGGC TCTCTCAGGA 
     1501 CCTTGCTTGG AGATGGTGTT GGCAGACAGC AGACAAGGGC CAAGCAAGTG TGCAATGTGG 
     1561 AGACGGGGCT TCAGACACAG GAGAGGCCGC CTGCAATGGC AGCCAAGCAC AGTGGCGGTG 
     1621 GACACTGCGT TCAGAACTTT CCCCCATCCC TGGCTTCAAA ACCCAGCCTG GGGCAGCAGA 
     1681 AACCAACCGC CTATGTGCGG CCAATGGATG GCCAGGACCA GGCTCCCGAC GAATCTCCTA 
     1741 AGCTTAAGTC ATCCACGGAA ACTGCTGTGC ACTGCACGGC CTACAGAGGG GTCCCGGCCG 
     1801 GCAAACCAGA GTCAGCCAGA GCCAAGGGCA AGCTCGCTAA GTTCAGCATC CCCAAGCAAG 
     1861 GCGAGGAGAA TAGATCTGGA GAAACCAACA GCTGTGTGGA AGAAATAATT CGGGAGATGA 
     1921 CCTGGCTTCC TCCACTTTCC GCCATTCAAG CTCCTGCCAA GGTGGAACCA AGCAAGTTCC 
     1981 CATTCCCAAA TAAGGACTCA CAACTTGTAT CCTCTGGACA CAGTAATCCA AAGAAAGCTG 
     2041 ATGCGGAGCC AGAGAGTCCA GACAATGGCA CGTCGAACAC TTCAACTCTG GAAGACGACC 
     2101 TTAAGCTAAG CAGTGATGAC GAAGAGGGTG AGCAGGCAGC CCAGAGAACG GTTCTCCGCG 
     2161 CTCTAGCTGA CAGCTCCGTG GTCCAGCAGA CCAACTGTAG AGGTTCCGCG CCATCCAGCA 
     2221 AGGGCAGCAG CAGCAGCAGC GGCGGCAGCA GCTCCTCCAG CGACTCAGAA AGCACTTCCG 
     2281 GCTCCGACTC CGAGACGGAG AGCAGCTCCA GCGAGAGCGA GAGTGAGGGC AGCAAGCCTC 
     2341 CGCACTGCTC CAGCCCCGAG GCCGAACCTG CGTCCTCGAA CAAGTGGCAG TTGGATAAAT 
     2401 GGCTGAACAA AGTTAATCCT CACAAGCCGC CAATTCTGAT CCAAAATGAA AGCCATGGCC 
     2461 CGGAAAGGAA TCAATACTAC ACGCCTGTGA AAGATGAAGG GCAAGACTGC GGGAAACTTC 
     2521 CTGAGATCTG CCAAGCCAGC CTGAGGGACA AGGAGCTCAA GACTGCCTGC AAGGAGGAGC 
     2581 AGCGGCCGAG GACCGCAAAC AAGGTCCCTG GCAGCAAAAG TGTCAAGCAA AAGTCCCCGC 
     2641 CCGCTGCGGT GGCTGTGACC GCAGCTGCCC CGCCGCCCGC GGTGCCCAGC GCACCCACGG 
     2701 AGAGTGCACC CGCGCCTACC CGCAGGTCAG CAGGCAAGAA GCCCACCAGG CGCACCGAGA 
     2761 GGACCTCAGC CGGGGACAGT GCCAACTGCC ACCGACCAGA GGAGCCAGTG GCCCCAGACG 
     2821 CGCTGGGGGC GAGCGTAGTG GGACCCCTAG AGCCCCCCAA AACCAGGCCC TGTGGTAACA 
     2881 GCAGGACAGG CCACCGCAAG GAGCTGCGCT CCTCAGTGAC CTGTGAGAAG CGTCGCACGC 
     2941 GGGGGCTGAG CAGAATTGTC CCTAAATCCA AGGAGTTTAT TGAAACAGAA TCTTCGTCTT 
     3001 CCTCTTCCTC CTCGGACTCA GACCTGGAGT CAGAGCAAGA GGAGTACATC CTGTCCAAGG 
     3061 CTCCAAGCAC CACCGGGAGT GAACAGAGGC TAAAGGAGGC CACCAGCAAC AACAGCAGCA 
     3121 GCAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCACCTCCC 
     3181 GAGCCTCTGT GGGCTCCATC AACGCCAGGA CCACCAGTGA CATTGCCAAG GAGCTAGAGG 
     3241 AACAGTTCTA CACGCTGGTC CCCTTTGGCA GGAATGAACT GCTTTCTCCA CTAAAGGACA 
     3301 GTGATGAGGT CAGGTCTCTC TGGGTCAAAA TTGACCTGAC ACTCCTTTCC AGGATCCCAG 
     3361 AACACCTGTC CCAGGAGCCT GGGGTCTTGA GCGCTCCTGC AGCCAAGGAC ACTGACAGTA 
     3421 CCCCAGCCAG CCATGCATTG GACACACCTG CTGAAAAGAC TTTGCCAAAA TCCAAGAGGA 
     3481 AACGCAAGTG TGACAATGAA GACGACTACA GGGAGATAAA GAAGGCCCAG GGAAAGAAGG 
     3541 AGGGTGCCTC ACGCCTGGCT GCCTCCACTA ACAACACCTT CTCCGGCAGC CACTGCAACG 
     3601 TGAGCATCAA CAGCTTGGCA ATACCAATAA ACAAAAATGA AAAGATGCTC CGGTCACCCA 
     3661 GCTCGCCACT CTCTGATGCA TGTAAACACA AATACTCCAG CGAGGACGTG ACTTCTTCCA 
     3721 GCCGACCTCA CGGCAACGGG TTGTTGACTT CTGTGTCTTC CAACAAAGAG CCTAAGGCCG 
     3781 AGAGCCAGCT GCAGTCGCAT GCTGGGGACC TCACGAAGAT CTCTCACAAC AGTTCCGAAA 
     3841 ATGGCATCCT CCACAGCAAA TCACGGCCCC AGACTGAGCC ATGGTCTCCA GGTTCCAACG 
     3901 GCCACAGGGA CTGCAAGAGA CAGAAACTGA TCTTCGATGA CATGCCCCGA AGTGCGGATT 
     3961 ATTTCATGCG AGAAGCTAAG CGGATGAAAC ACAAAGCAGA CGCCATGGTG GAGAAGTTTG 
     4021 GGAAGGCCCT CAACTATGCC GAAGCAGCCC TGTCCTTCAT CGAGTGTGGA AACGCAATGG 
     4081 AACAAGGGCC GATGGAGTCC AAGTCTCCGT ACACCATGTA CTCGGAGACA GTGGAGCTCA 
     4141 TCAGGTATGC TATGAGACTA AAAACCCACT CAGGCCCCAA TGCCACACCG GAGGACAAAC 
     4201 AGTTAGCTGC GCTTTGTTAC CGATGCCTGG CTCTCCTATA CTGGCGGATG TTTCGACTCA 
     4261 AAAGGGACCA TGCTGTCAAG TATTCAAAAG CACTCATCGA CTATTTCAAG AATTCATCGA 
     4321 AAGCTGCCCA AGCCCCATCC CCATGGGGGT CTAGCGGCAA GAGCACTGGA AGCCCATCCC 
     4381 CCATGTCTCC CAACCCTTCT CCCGCCAGCT CCGTGGGGTC TCAGGGCAGC CTTTCCAGTT 
     4441 CCAGCGGCCT GTCCCCATCG ACCATTGTGA GCATCCCCCA GCGAATTCAC CAGATGGCGG 
     4501 CCAATCACGT CAGCATCACC AACAGCATCC TCCACAGCTA CGACTACTGG GAAATGGCTG 
     4561 ATAACCTGGC CAAGGAAAAC AGAGAGTTCT TCAATGACCT GGATTTGCTC ATGGGACCTG 
     4621 TCACCCTGCA CAGCAGCATG GAGCACCTGG TCCAGTACTC CCAGCAAGGC CTGCACTGGT 
     4681 TACGGAGCAG CACACACCTG TCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4741 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4801 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4861 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4921 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4981 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5041 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5101 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5161 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5221 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5281 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5341 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5401 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5461 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5521 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5581 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5641 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5701 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5761 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5821 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5881 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5941 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6001 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6061 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6121 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6181 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6241 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6301 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6361 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6421 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6481 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6541 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6601 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6661 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6721 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6781 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6841 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6901 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6961 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7021 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7081 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7141 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7201 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7261 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7321 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7381 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7441 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7501 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7561 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7621 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7681 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7741 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7801 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7861 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7981 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8041 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8101 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8161 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8221 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8281 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8341 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8401 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8461 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8521 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//