LOCUS       RR217550                8538 bp    DNA     circular  SYN 
DEFINITION  Adamts18 (myc-DDK-tagged) - Rat ADAM metallopeptidase with thrombospondin type 1 motif, 18 (Adamts18)
ACCESSION   NM_001191944
VERSION     NM_001191944.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4695..4786 
                     /label="Myc-DDK"  
     misc_feature    4816..5401 
                     /label="PolyA signal"  
     misc_feature    4873..4892 
                     /label="Restriction Enzyme"  
     misc_feature    5677..6349 
                     /label="ColE1"  
     misc_feature    6779..7573 
                     /label="Kan/Neo"  
     misc_feature    7608..7910 
                     /label="SV40 ori"  
     misc_feature    7996..8473 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4677..4683 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4677 
                     /label="Adamts18"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTGCGCC CTCCTGTTCC TGTGCGCCCT CCGGGCCGCC GGTCCTGGGC 
     1081 CGCCGTGGGG GCCCGCGGGA CTGGCGCGTC TGGCCAAGGC GCTCCAGCTG TGCTGCCTCT 
     1141 GCTGTGCTTC GGTGGCCGTC GCCTTAGCCA GTGACAGCGG CAGCAGCGGT GGCAGCGGAT 
     1201 TAAATGATGA TTACGTCTTT GTCGTGCCAG TAGAAGTGGA CTCGGGCGGG TCATATATTT 
     1261 CGCACGACAT TTTGCACAAC AGGAAAAGGC GATCGGCACA CGGTGCCAGC AGCTCCGTGC 
     1321 ACTACCGCTT TTCAGCATTT GGACAGGATC TACACTTAGA GCTTAAGCCC TCGGCGCTTT 
     1381 TGAGCAGTCA CTTTATAGTC CAGGTACTTG GAAAGGATGG TGCTTCAGAG ACTCGGGAGC 
     1441 CTGCGGTGGT ACAGCAGTGT TTGTATCAGG GATTTATCAG AAATGACAGC CTGTCCTCTG 
     1501 TTGCTGTGTC TACGTGTGCC GGCTTGTCAG GTCTGATAAG GACACGAGAC AATGAATTCC 
     1561 TCATCTCACC GCTACCTCAG CTGCTGGCCC AGCAACACAA CTACAGCTTG CCTGCAGGCC 
     1621 ACCATCCTCA CGTCCTGTAC AAAAGGACAG CAGAGAAGAG GGTCAGGAGG TATCAAGACT 
     1681 ACCCTGCCTC CCAGCGGACA TATCCTGGTC ACTCCCCAAG TCACACCCCC CCTGCCTCCC 
     1741 AGAGCCAAGA GACAGAGTAC CGCCATCGAA GGTGGCAAAA GCAGCATTTT TGTGGACGAC 
     1801 GCAAGAAATA TGCCCCCAAA CCTCCTGCAG AAGATGCCTA TCTACGTTTT GATGAATATG 
     1861 GAGGCGCAGG ACGGCCCAGA CGATCAGCCA GAAAGTCTCA AAAGGGTCTC AACGTGGAGA 
     1921 CCCTCGTGGT GGCAGATGCA AAGATGGTGG AGAAGCACGG CAAGGATGAC GTCACTACGT 
     1981 ACATCCTCAC AGTCATGAAC ATGGTTTCTA GCCTGTTCAA AGATGGGACC ATTGGAAGTG 
     2041 ACATAAACAT TGTGGTTGTG AGCCTAATTC TGTTGGAAGA CGAACCTGGA GGATTGCTGA 
     2101 TCAATCACCA TGCAGACCAG TCTCTAAATA GCTTCTGTCA ATGGCAGTCA GCCCTTGTGG 
     2161 GAAAGAACGG CAAGAGACAC GACCATGCCA TCCTCCTCAC AGGATTTGAC ATTTGTTCCT 
     2221 GGAAGAACGA ACCGTGTGAC ACCCTAGGAT TTGCACCTAT CAGTGGGATG TGCAGTAAAT 
     2281 ACCGAAGCTG TACCATCAAT GAAGACACAG GACTTGGCCT TGCCTTCACC ATTGCACATG 
     2341 AGTCAGGGCA CAACTTTGGC ATGGTTCATG ATGGCGAAGG CAACCCCTGC AGGAAGGCGG 
     2401 AAGGCAACAT CATGTCCCCC ACGCTGACCG GAAACAATGG GGTGTTTTCA TGGTCCTCCT 
     2461 GCAGCCGTCA GTATCTAAAG AAATTCCTCA GTACACCACA GGCTGGCTGT CTGGTGGATG 
     2521 AGCCCAAGCA AACGGGACAG TATAAATATC CGGACAAACT CCCAGGACAG ATTTATGATG 
     2581 CCGACACGCA GTGTAAGTGG CAATTTGGAG CCAAAGCCAA GCTGTGCAGC CTTGGGGTTA 
     2641 TGAAGGACAT TTGCAAGTCG CTCTGGTGCC ACCGCGTGGG CCACAGATGT GAAACCAAGT 
     2701 TCATGCCTGC AGCCGAGGGG ACCGTCTGTG GCTTGAGCAT GTGGTGTCGG CAAGGCCAAT 
     2761 GTGTAAGGCT TGGAGATCTC GGGCCCCGGC CCATCCACGG CCAGTGGTCT GCCTGGTCGA 
     2821 AGTGGTCAGA ATGTTCTCGA ACATGCGGTG GAGGAGTCAA GTACCAGGAG AGACACTGTA 
     2881 GTAACCCCAA GCCTCAGTAT GGTGGGAAGT ACTGTCCAGG ATCTAGCCGC ATCTACAAGC 
     2941 TGTGCAACAT TAACCCTTGT CATGAGAATA GCTTGGATTT CCGTGCCCAG CAATGTGCAG 
     3001 AGTATAACAA CAAGCCCTTC CGGGGATGGT TGTACCGTTG GAGACCCTAT ACAAAAGTTG 
     3061 AAGAGGAAGA TCGATGTAAA CTCTACTGCA AGGCTGAGAA CTTTGAGTTT TTTTTCGCCA 
     3121 TGTCTGGCAA GGTGAAAGAC GGGACGCCTT GCTCCCCACA CAGAAATGAT GTCTGCATCG 
     3181 ACGGGATTTG TGAACCTGTG GGATGCGACC ATGAACTTGG CTCTAAGGCA GTGTCAGATG 
     3241 CATGCGGTGT TTGCAAAGGC GATAACTCGA CTTGCAAGTT TTATAAAGGC CTGTACCTCA 
     3301 GTCAGCACAA AGCCAACGAG TATTATCCTG TGGTCACTAT CCCCGCTGGG GCCCGAAGCA 
     3361 TTGAAATTCA GGAGTTGGAG CTTTCTTCCA GCTATCTTGC TGTTCGAAGC CTCAGTCAAA 
     3421 AGTATTACCT CACAGGGGGC TGGAACATCG ACTGGCCTGG GGACTTCACC TTCGCCGGCA 
     3481 CCACATTTGA ATACCAGCGT CCCTTTAACC GCCCTGAACG ACTGTACGCG CCAGGACCCA 
     3541 CAAATGAGAC GCTGGTCTTT GAAATTCTGA CCCAAGGCAA GAACCCCGGG ATCACTTGGA 
     3601 AGTACGCACT TCCCAAGGTC ATGAATGTAA CTCAGCCAGC GGCCAAGAGA CACCACCACA 
     3661 CCTGGCGCAC AGTACAGTCT GACTGCTCGG TCACCTGCGG CGGAGGTTAC ATAAGCATCA 
     3721 AGGCCATTTG CTTACGAGAT CAAAACACCC AAGTCAATTC CTCATTCTGC AGTGTAAGAA 
     3781 CCAAGCCAGC AACGGAACCC AAGCTATGCA ATGCTTTCTC CTGCCCAGCC TATTGGTTGC 
     3841 CAGGTGAGTG GAGTACATGC AGCAAGTCCT GTGCCGGGGG CCAGCAGAGC CGCAAGATCC 
     3901 GGTGTGTGCA GAAGAAGCCC TTCCAGAAAG AGGAGGCTGT GCTGCATTCT CTCTGTCCAG 
     3961 TGAGCACGCC TACCCAGGTT CAAGTCTGCA ACAGCCATGC CTGCCCTCCA GAGTGGAGCC 
     4021 CCGGGCCCTG GTCTCAGTGT TCCAAGACCT GTGGCCGGGG AGTGAGGAGA CGTGAGGTCC 
     4081 TCTGTACAAG CCCTGCAGCC GAGACACTCC CCGAGAGCCT GTGTTCCAGC AGCCCCAAAC 
     4141 CCGAGGCACA GGAGGGCTGT GTGCTGGGAC GGTGCCCCAA GAACAACAGA CTGCAGTGGA 
     4201 TCGCCTCTGC CTGGACTGAG TGTTCTGCAA CCTGTGGCTT GGGTGTGAGG AAGAGAGAAT 
     4261 TCAAGTGCAT GGAGAAGACC CTGCAGGGAA AGCTGATGAC GTTCCCAGAG CGGAGATGTC 
     4321 GCAACATTAA GAAGCCAAAC CTGGAACTGG AAGAAACCTG CAACCGAAGG GCTTGCCCAG 
     4381 TATACAGCAT GGCGGCAGGC TGGTATTCGT CCCCGTGGCA ACAGTGCACT GTAACCTGTG 
     4441 GGGGAGGGGT CCAGACCCGA TCTGTCCACT GCATGCAGCA AGGCCGGCCT TCCTCAAGTT 
     4501 GTCTGCTCCA TCAGAAGCCT CCGGTGCTAA GAGCCTGTAA TACCAACTTC TGTCCAGTTC 
     4561 CTGAAAAGAG AGATGATCCG TCGTGTGTGG ATTTCTTCAG CTGGTGTCGC CTGGTCCCTC 
     4621 AGCACGGAGT CTGCAACCAC AAATTTTATG GAAAACAGTG CTGTCGATCA TGCTCAAGGA 
     4681 AGAGCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4741 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4801 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4861 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4921 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4981 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5041 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5101 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5161 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5221 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5281 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5341 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5401 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5461 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5521 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5581 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5641 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5701 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5761 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5821 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5881 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5941 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6001 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6061 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6121 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6181 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6241 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6301 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6361 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6421 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6481 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6541 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6601 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6661 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6721 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6781 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6841 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6901 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6961 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7021 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7081 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7141 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7201 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7261 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7321 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7381 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7441 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7501 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7561 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7621 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7681 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7741 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8101 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8161 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8221 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8281 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8341 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8401 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8461 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8521 AAATTTAACG CGAATTTT
//