LOCUS       RR217544                8499 bp    DNA     circular  SYN 
DEFINITION  Gpr158 (myc-DDK-tagged) - Rat G protein-coupled receptor 158 (Gpr158)
ACCESSION   NM_001170326
VERSION     NM_001170326.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4656..4747 
                     /label="Myc-DDK"  
     misc_feature    4777..5362 
                     /label="PolyA signal"  
     misc_feature    4834..4853 
                     /label="Restriction Enzyme"  
     misc_feature    5638..6310 
                     /label="ColE1"  
     misc_feature    6740..7534 
                     /label="Kan/Neo"  
     misc_feature    7569..7871 
                     /label="SV40 ori"  
     misc_feature    7957..8434 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4638..4644 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4638 
                     /label="Gpr158"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGCCATG GCTTACTCTT TACTCCTCTG CCTCCTGCTC GCTCATCTGG 
     1081 GATTGGGAGA GGTTGGTGCC AGCCTCGACC CTTCAGAACG GCCAGATTCT TCTCGAGAGA 
     1141 GGACTTCGAG GGGGAAGCAG CACGGTCAGC AGCTGCCGCG AGCCTCTGCC CCGGACCCCT 
     1201 CTATTCCCTG GAGCCGCTCC ACAGACGGCA CCATCTTGGC ACAAAAACTA GCCGAGGAGG 
     1261 TGCCCATGGA CGTGGCCTCT TACCTCTATA CTGGGGACTT CCACCAGCTG AAGAGAGCCA 
     1321 ACTGCTCAGG CCGCTATGAG TTGGCGGGCC TGCCGGGGAA GTCACCGTCC CTAGCCAGCT 
     1381 CCCATCCTTC CTTGCACGGG GCGCTGGACA CTCTGACACA CGCCACCAAC TTCCTCAACA 
     1441 TGATGTTGCA GAGCAATAAG TCTCGGGAGC AGACCGTGCA GGATGACCTG CAGTGGTACC 
     1501 AGGCTTTGGT GCGAAGTCTC CTGGAGGGAG AGCCCAGCAT CTCCCGGGCG GCCATCACCT 
     1561 TCAGCACCGA GTCGCTGTCC ACGCCCGCCC CGCAAGTCTT CCTCCAGGCC ACCCGTGAGG 
     1621 AGAGTCGCAT CCTGCTGCAG GACCTGTCCT CCTCCGCTCA CCACCTGGCT AACGCCACTC 
     1681 TGGAGACCGA GTGGTTCCAC GGGCTTCGGC GAAAGTGGAG GCCCCACTTG CACCGCCGCG 
     1741 GCTCCAATCA GGGGCCACGG GGCCTGGGCC ACAGCTGGCG GCGCAGGGAT GGGCTTGGCG 
     1801 GGGACAGGAG CCATGTCAAG TGGTCTCCAC CTTTTCTGGA GTGCGAGAAT GGGAGTTACA 
     1861 AGCCCGGGTG GCTGGTCACT CTCTCTGCTG CCTTCTACGG GCTTCAACCT AACCTGGTCC 
     1921 CGGAATTCAG GGGCGTGATG AAAGTTGATA TAAATCTTCA GAAAGTGGAC ATTGACCAAT 
     1981 GTTCGAGTGA TGGCTGGTTT TCAGGAACCC ACAAATGTCA CCTTAACAAC TCAGAGTGTA 
     2041 TGCCAATCAA AGGCCTAGGA TTCGTGCTCG GAGCCTATCA GTGCGTTTGC AAAGCAGGAT 
     2101 TCTATCATCC CCGTGTCTTC TCAGTGAACA ACTTTCAGAG AAGAGGTCCA GATCACCATT 
     2161 TTTCAGGAAG CACAAAGGAT GTGTCAGAAG AAGCCCACGT CTGCCTGCCC TGCCGAGAAG 
     2221 GCTGCCCCTT CTGTGCTGAT GACCGCCCAT GTTTTGTCCA GGAGGATAAG TACCTGCGTC 
     2281 TCGCCATCAT CTCCTTCCAA GCCCTGTGTA TGCTGCTGGA CTTTGTCAGC ATGCTGGTGG 
     2341 TCTACCACTT CCGCAAAGCA AAGAGCATCA GGGCATCTGG CCTCATCTTG TTGGAAACAA 
     2401 TACTCTTTGG CTCTCTGCTT CTTTACTTTC CGGTTGTTAT TCTGTACTTT GAGCCAAGTA 
     2461 CATTTCGTTG TATTCTCCTG AGGTGGGTTC GTCTTCTCGG TTTTGCGACA GTTTATGGAA 
     2521 CTGTGACCCT CAAGCTTCAC AGGGTTTTGA AGGTATTCCT TTCAAGAACA GCACAAAGAA 
     2581 TCCCATATAT GACTGGCGGT CGGGTCATGA GGATGCTGGC AGTAATAGTC TTGGTAGTGT 
     2641 TTTGGTTTCT GGTTGGCTGG ACTTCTTCTA TGTGCCAGAA CTTGGAGCGG GATATCTTGC 
     2701 TTGTTGGCCA AGGGCAAACA TCTGATAACC TCACCTTCAA TATGTGCCTC ATTGACCGCT 
     2761 GGGATTACAT GACAGCAGTT GCTGAATTTT TATTCCTCCT GTGGGGCATT TATCTCTGCT 
     2821 ATGCAGTGCG GACTGTGCCG TCAGCATTCC ATGAGCCACG CTACATGGCT GTCGCAGTGC 
     2881 ACAACGAGCT CATCATCACT GCTATATTCC ACACAATCAG ATTCGTGCTT GCTTCGAGGC 
     2941 TTCAGCCTGA CTGGATGCTG ATGCTGTACT TTGCACACAC TCACTTGACT GTGACAGTCA 
     3001 CCATCGGGCT GCTTCTGATT CCAAAGTTTT CACACTCAAG CAATAATCCC CGTGATGACA 
     3061 TCGCCACAGA AGCGTATGAA GATGAGTTGG ACATGGGTCG TTCTGGATCC TACCTGAACA 
     3121 GCAGCATCAA TTCAGCCTGG AGCGAGCACA GTCTAGATCC AGAAGACATT CGGGATGAGC 
     3181 TGAAGAAACT CTATGCCCAG TTAGAGATAT ACAAACGTAA GAAGATGATC ACAAACAACC 
     3241 CCCACCTCCA GAAAAAGCGG TGCTCCAAGA AGGGCTTAGG CCGGTCCATC ATGAGGCGTA 
     3301 TCACTGAGAT CCCAGAGACG GTCAGCAGGC AGTGCTCCAA AGAGGACAAG GAGGGCACAG 
     3361 ATCACAGTGC AGCCAAAGGG ACAGGCCTTG TCCGGAAGAA CCCTACTGAG TCCTCAGGGA 
     3421 ACACAGGGAG ACCCAAGGAG GAGTCCCTGA AAAACCGGGT CTTTTCTCTC AAGAAATCCC 
     3481 ATAGCACATA TGACCATGTA AGAGACCAAA CTGATGAATC CAGCAGCCTG CCCACAGAGA 
     3541 GCCAGGAGGA GGAGGTCACG GAAAATTCAA CCTTGGAGTC GCTGTCAAGT AAAAAACTGA 
     3601 CACAAAAAGT CAAAGAAGAT AGCGAGGCCG AGTCCACAGA GTCAGTGCCC TTGGTCTGCA 
     3661 AGTCAGCCAG TGCTCACAAC CTCAGCTCTG AAAAGAAGCC CGGGCACCCA CGCACATCTA 
     3721 TGTTACAGAA GTCGCTCAGT GTCATTGCAA GTGCCAAGGA GAAGACACTA GGGCTGGCTG 
     3781 GTAAAACCCA GACTTTAGTT ATGGAAGACC GAGCTAAGTC CCAGAAACCT CAGCCAAAAG 
     3841 ATAGAGAGAC AAACAGGAAG TACTCAAATT CCGATAACAC AGAGACTAAA GATTCTGGCT 
     3901 GCCCAAACTC TAATCACACA GAGGAGCTAA GAAAGCCCCA GAAATCTGGG ATTATGAAAC 
     3961 AGCAGAGGGT CAACCTCCCC ACTGCCAATC CTGACGCGAG CTCCAGCACC ACCCAGATAA 
     4021 AGGACAATTT TGATATTGGA GAAGTGTGCC CTTGGGAGGT TTATGACCTG ACCCCCGGTC 
     4081 CTGTGCCTTC AGAACCAAAA GCTCAAAAAC ACGTATCAAT TGCAGCTTCG GAAGTGGAAC 
     4141 AAAACCCAGC TTCTTTTTCG AAGGAGAAGT CCCACCACAA GCCTAAGGCA GCTGAAGGTC 
     4201 TCTACCAAGC CAATCACAAG AGCATAGACA AGACAGAGGT GTGCCCCTGG GAGAGCCACG 
     4261 GTCAGTCCCC TTTGGAAGAT GAGAATCGTC TGATTTCTAA GACTCCAGTT CTCCCAGGGA 
     4321 GAGCCAGAGA AGAGAATGGA AGTCAGTTGT ACACAACCAA TATGTGTGCT GGGCAATATG 
     4381 AAGAACTGCC CCCCAAAGCC GTAGCATCAA AAGTAGAGAA TGAAAATCTC AACCAAATGG 
     4441 GAGACCAGGA GAAACAGACA TCTTCTTCTG TGGATATCAT CCCTGGCTCC TGTATCTCGA 
     4501 GTAATAACTC CCCTCAACCC TTAACGTCAC GAGCCGAAGT GTGTCCCTGG GAGTTCGAGC 
     4561 CCCTAGAACA ACCAAATGCT GAAAGAATCG TAGCTTTACC CGCCTCCTCT GCTTTAAGTG 
     4621 CAAGTAAGAT ACCCGGGCCA CGGAAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4681 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4741 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4801 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4861 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4921 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4981 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5041 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5101 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5161 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5221 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5281 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5341 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5401 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5461 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5521 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5581 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5641 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5701 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5761 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5821 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5881 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5941 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6001 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6061 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6121 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6181 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6241 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6301 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6361 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6421 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6481 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6541 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6601 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6661 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6721 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6781 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6841 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6901 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6961 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7021 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7081 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7141 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7201 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7261 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7321 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7381 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7441 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7501 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7561 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7621 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7681 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7741 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7861 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7921 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7981 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8041 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8101 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8161 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8221 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8281 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8341 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8401 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8461 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//