LOCUS       RR217538                8457 bp    DNA     circular  SYN 
DEFINITION  Lamc2 (myc-DDK-tagged) - Rat laminin, gamma 2 (Lamc2)
ACCESSION   NM_001100640
VERSION     NM_001100640.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4614..4705 
                     /label="Myc-DDK"  
     misc_feature    4735..5320 
                     /label="PolyA signal"  
     misc_feature    4792..4811 
                     /label="Restriction Enzyme"  
     misc_feature    5596..6268 
                     /label="ColE1"  
     misc_feature    6698..7492 
                     /label="Kan/Neo"  
     misc_feature    7527..7829 
                     /label="SV40 ori"  
     misc_feature    7915..8392 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4596..4602 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4596 
                     /label="Lamc2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGCGCTC TGGCTCAGCT GCTGCCTCGG GGTCGCGCTC CTGCTGCCAG 
     1081 CAGCCCAGGC CACCTCCAGG AGAGAAGTCT GTGATTGCAA TGGGAAGTCC AGGCAGTGCG 
     1141 TCTTTGACCA GGAGCTCCAT CGACAGACAG GCAATGGGTT CCGTTGCCTC AACTGCAATG 
     1201 ACAATACGGC CGGGGTTCAC TGCGAGAGGT GCAGGGAGGG GTTTTACCGG CAGCGAGACA 
     1261 GAGATCGCTG CCTGCCCTGC AACTGCCACT CAAAAGGTTC CCTCGGTGCT GGATGTGACA 
     1321 ACTCTGGACA ATGCAGGTGT AAGCCAGGTG TGGTAGGACA GAGGTGTGAC CGATGTCAGC 
     1381 CAGGCTTCCA TATGCTCACC GATGCTGGAT GTACCCGAGA CCAGAGGCAA CTAGATTCCA 
     1441 AGTGTGACTG TGACCCAGCC GGCATCTCTG GACCCTGTGA TTCTGGCCGA TGTGTGTGCA 
     1501 AACCAGCCGT CACTGGAGAA CGCTGTGACA GGTGCCGAGC AGGCTACTAT CATCTGGACA 
     1561 GGGCAAACCC TGAGGGCTGC ACCCAGTGTT TCTGCTATGG GCATTCAGAC AGCTGCCACA 
     1621 CCTCTGCTGA CTTCAGTGTC CACAAAATCA CCTCAACTTT CAATCAGGAT GAAGATGGTT 
     1681 GGAAAGCAGT TCAGAGAAAT TGGGCGCCTG CAAAACTCCA CTGGTCACCG CGCCATCAGG 
     1741 ATGTATTTAG TTCTGCCCGA AGATCAGACC CAGTCTATTT CGTGGCACCC GCCAAATTTC 
     1801 TCGGTAATCA GCAAGTGAGT TACGGGCAGA GCCTGTCTTT TGACTACCGC GTGGACAGAG 
     1861 GAGGTCGACA CCCATCTGCC TATGATGTGA TCCTGGAAGG TGCTGGTCTA CAGATCAGAG 
     1921 CTCCTCTGAT GCCACCAGGC AAGACACTTC CCTGTGGGAT CACAAAGACT TACACGTTCA 
     1981 GATTAAACGA ACATCCAAGC AGTCACTGGA GCCCCCAGCT GAGTTATTTC GAGTATCGAA 
     2041 GGTTACTGCG GAACCTCACA GCCCTCCTCA TCCGAGCTAC GTACGGAGAA TACAGTACAG 
     2101 GGTACATTGA CAATGTGACC CTGATTTCAG CCCGCCCTGT CTCTGGAGCC CCAGCACCCT 
     2161 GGGTTGAACG TTGTGTATGC CCTGCTGGGT ACAAGGGACA ATTCTGCCAG GAATGTGCTT 
     2221 CTGGTTACAA AAGAGATTCA GCAAGACTGG GGCCTTTTGG CACCTGTGTT CCCTGTAACT 
     2281 GCCAAGGGGG AGGGGCCTGT GACCCAGACA CAGGGGATTG CTACTCAGGG GACGAGAATC 
     2341 CTGACATTGA GTGTGCCGAC TGCCCCATTG GTTTCTATAA CGACCCGCAC GACCCCCGCA 
     2401 GCTGCAAGCC GTGTCCCTGC CACAATGGCT TCAGCTGTTC CGTGATGCCT GAGACAGAGG 
     2461 AGGTGGTGTG CAACAACTGC CCCCCTGGGG TCACAGGTGC CCGCTGTGAG CTCTGTGCAG 
     2521 ATGGCTTCTT TGGGGACCCC TTTGGGGAAC GTGGCCCAGT GAGGCCTTGT CAGCGGTGCC 
     2581 AATGCAACAA CAATGTGGAC CCCAGTGCTT CTGGGAACTG TGACCAGTTG ACAGGCAGAT 
     2641 GTTTGAAATG CATCTACAAC ACAGCGGGAA TCTACTGTGA CCAGTGCAAA GCAGGTTACT 
     2701 TCGGAGACCC ATTGGCTCCC AACCCAGCAG ACAAGTGTCG AGCTTGCAAC TGCAACCCTG 
     2761 TGGGCTCGGA GCCTGGAGAG TGTCGAAGTG ATGGCAGCTG TGTTTGCAAG CCAGGCTTTG 
     2821 GAGGCCTCAA CTGTGCGCAC GCCGCCGCCC TAACCAGTTG CCCTGCTTGC TACAATCAAG 
     2881 TGAAGACTCA GATGGACCAG TTTGCCCAGC AGCTCCAGAA CTTGGAGGCC CTGGTTTCAA 
     2941 AGGCTCAGGG TGGTAATGGT GCAGTCCCCA GCGAGGAGCT GGAAGGCAGG ATGCAGCAGG 
     3001 CTGAGCAGGC CCTTCGAGAC ATTCTGAGAG AAGCTCAGAT TTCAGAAGGT GCTATGAGAG 
     3061 CCCTCAGCCT CCAGCTGGCC AAGGCAAGGA GCCAAGAGAA CAACTACAAG AACCGCCTGG 
     3121 ATGACCTCAA GATGACTGTG GAAAGGATTC GGGCCCTGGG CAGTCAGTAT CAGAACCGAG 
     3181 TTCAGGATAC AAGCAGACTC ATCTCTCAGA TGCGCCTGAG CCTGGCAGAA AGTGAAGCTT 
     3241 CCCTGCAAAA CACTAACATC CATTCCTCTG AGCACTACGT GGGGCCAAAT GGATTTAAAA 
     3301 GTCTGGCTCA GGAGGCCGCA AGATTGGCAG ATAGCCATGT TGAGTCAGCT AACACAATGA 
     3361 AGCAGCTAAC GAGGGAAACC GAGGACTATT CCAAACAAGC ACTTTCATTG GCCCGCAAGG 
     3421 CTCTGAGTGG AGGAGGTGGA AGCGGTGTCC TGGACAGCTC CGTGGTACAA GGTCTTATGG 
     3481 GAAAATTAGA GAAAACCAAG TCCCTGACCC AGCAGTTGTC AAGGGAGGGC ACCCAAGCCG 
     3541 ACATCGAAGC TGATAGGTCC TATCAACATA GTCTCCGCCT CCTGGATTCA GCGTCTCAGC 
     3601 TTCAGGGAAT CCGTGATTCC TCTTTTCAGG CAGAGGCAAA GAGGATCAGG CAGAAGGCTG 
     3661 ATTCCCTCTC CAACTTGGTG ACCAAACAAA TGGATGCATT CACGAGTGTG CGAAACAATC 
     3721 TGGGGAACTG GGAAAAAGAA ACACGGCAGC TCTTGCAGAC TGGGAAGGAC AGAAGACAGA 
     3781 CTTCGGATCA GCTGCTTTCC CGTGCCAACC TTGCTAAAAG CAGAGCCCAA GAAGCGCTAA 
     3841 GTATGGGCAA TGCCACTTTT TATGAAGTTG AGAACATCCT AAAGAACCTC AGAGAGTTTG 
     3901 ATCTGCAGGT TGAAGACAGA AAAGCAGAGG CTGAGGAGGC CATGAAGAGA CTCTCCTATA 
     3961 TTAGCCAGAA GGTTGCAGAT GCCAGTGACA AGACCCAGCA AGCAGAAACG GCCCTGGGGA 
     4021 GCGCCACTGC TGACACCCAA CGGGCAAAGA ACGCAGCTAC GGAGGCCCTA GAGATCACCA 
     4081 GCGAGATAGA GCAGGAGATA GGGAGTCTGA ACTTGGAAGC CAATGTGACA GCAGATGGGG 
     4141 CCTTGGCCAT GGAAAAGGGA CTGGCCACTC TGAAGAGCGA GATGAGAGAG GTGGAAGGGG 
     4201 AGTTGGCCAG GAAGGAGCTT GAGTTCCACA CGGATAAAGA CTCCTTGCAG CTGGTGATTA 
     4261 CTGAAGCCCA GCAAGCTGAT GCCAGAGCCA AGAGCACGGG AGTTACGATC CAAGACACGC 
     4321 TCAACACATT GGAAGGCATC CTGCGCCTAA TAGACCAGCC TGATGCTGTG GATGAAGAGG 
     4381 GGCTGATGCT GTTAGAACAA GAGCTTTTCC AAGCCAAGAC CCAGATCAAC AGTCGACTTC 
     4441 GGCCCTTGAT GTCTGAACTG GAGGACAGGG CGCGTCGACA GAGCAACCAC CTCCACCTGC 
     4501 TGGAGACCAG CATAGATGGG ATTCTAGCCG ATGTGAAGAA CCTGGAGAAC ATTCGAGACA 
     4561 ACCTGCCCCC AGGCTGCTAC AACACCCAAG CTCTTGAGCA GCAGACGCGT ACGCGGCCGC 
     4621 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4681 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4741 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4801 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4861 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4921 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4981 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5041 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5101 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5161 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5221 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5281 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5341 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5401 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5461 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5521 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5581 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5641 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5701 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5761 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5821 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5881 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5941 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6001 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6061 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6121 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6181 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6241 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6301 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6361 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6421 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6481 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6541 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6601 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6661 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6721 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6781 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6841 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6901 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6961 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7021 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7081 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7141 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7201 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7261 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7321 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7381 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7441 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7501 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7561 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7621 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7681 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7861 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7921 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7981 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8041 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8101 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8161 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8221 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8281 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8341 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8401 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//