LOCUS       RR217534                8439 bp    DNA     circular  SYN 
DEFINITION  Tyk2 (myc-DDK-tagged) - Rat tyrosine kinase 2 (Tyk2)
ACCESSION   NM_001257347
VERSION     NM_001257347.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4596..4687 
                     /label="Myc-DDK"  
     misc_feature    4717..5302 
                     /label="PolyA signal"  
     misc_feature    4774..4793 
                     /label="Restriction Enzyme"  
     misc_feature    5578..6250 
                     /label="ColE1"  
     misc_feature    6680..7474 
                     /label="Kan/Neo"  
     misc_feature    7509..7811 
                     /label="SV40 ori"  
     misc_feature    7897..8374 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4578..4584 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4578 
                     /label="Tyk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGACAAGC ATGCCTCTGT GTGGGCGGAG AGCCGCCTCG GAAGACAGCA 
     1081 AGGCAGATGG CACAGAAGCT CAGCCCCTGG TGCCCACAGG ACGGTTGATG GTGCTGCTGC 
     1141 ACTGGCCCGG GCCTGAGGGC GGGGAGCCCT GGGTCACCTT CAGCCAGCCA TCTCTGACTG 
     1201 CAGAGGAGAT GTGCATCCAC ATCGCACACA AAGTCGGCAT CACTCCACCC TGCCTGAATC 
     1261 TCTTCGCACT CTACGATGCA CAGGCCAAGG TCTGGCTGCC CCCAAACCAC GTTCTGGACA 
     1321 CATCCCAAGA CGCAACCCTG AGGCTCTATT TTCGAATGAG GTTTTACTTC CGGAACTGGC 
     1381 ATGGCATGAA TCCCCAAGAG CCTGCTGTAT ACCGATGTGG TTTCCCAGGG GCAGAGACTT 
     1441 CCTCAGACCA TGCAGAGCAA GGTGTACAGC TCTTGGACTC TGCCTCATTT GAATACCTTT 
     1501 TTGAGCAGGG AAAACATGAA TTCATGAACG ATGTGGTATC TCTGCGGGAC CTGTCTAGCG 
     1561 AGGAAGAGAT CCACCACTTT AAGAATGAGA GCCTGGGCAT GGCCTTTCTG CACCTCTGCC 
     1621 ATCTTGCTCT CAACCGTGGC GTCCCCCTGG AGGAGATGGC CAGAGAGATC AGCTTCAAGA 
     1681 ACTGTATCCC TCACTCCTTC CGTCAGCACA TCCGCCAGCA CAATGTGCTT ACGCGCCTGC 
     1741 GTCTCCGCAG AGTCTTCCGC CGCTTCCTGC AGGCCTTCCA GCCTGGCCAC CTCTCCCAAC 
     1801 AGGTTGTGAT GGTTAAGTAC TTGGCCACCC TGGAGCGGCT GGCTCCTCGT TTTGGCTCAG 
     1861 AGCACATACC TGTGTGTCAT CTGGAAGTTC TGGCCCAGCC TGAGAGGGAC CCCTGCTACA 
     1921 TCCAGAACAG TGGGCAGACC ACTGGGGACC CAGGCCCAGA GCTGGCTTCT GGGCCAGCCA 
     1981 CCCATGAGGT ACTGGTGACA GGCACCGGAG GTATCCAGTG GCGTCCACTA CAGACCCAGG 
     2041 ACTCTGAGAG AGGTCACAGC AGAGGGAATC CCCAAGGCAG CCAGTCTGGG AAGAAACCCA 
     2101 AGGCCCCTGA GTCTGGAGAG CACCTGGCAG GGAGTCCCCA GGAACCACCT TGGACCTACT 
     2161 TCTGTGACTT CCAGGACATT TCCCATGTGG TACTAAAGGA GCGTCGAGTG CACATCCACC 
     2221 TTCAAGACAA CAAGTGCTTG TTGCTGTGCC TCTGCTCCCA GGCCGAGGCC CTGTCCTTTG 
     2281 TGGCCCTGGT CGATGGCTAT TTCCGCTTGA CTGCTGACTC CAGCCACTAC CTGTGCCATG 
     2341 AGGTAGCACC CCCGAGGCTG GTGACTAGCA TCCAGAATGG CATCCACGGG CCCCTGATGG 
     2401 ACCCATTTGT ACAGGCTAAG CTGTGGCCAG AGGATGGCCT CTACCTGATC CAGTGGAGCA 
     2461 CCAGCCACCT GCACCGACTG ATCCTCACCG TAGCCCATCG AAACCCGGCT TCCAGAAACG 
     2521 GCCCTAGGGG CCTACGCCTC CGAAAGTTCC CCATCACCCA GCAGCCTGGA GCCTTTGTGC 
     2581 TTGATGGTTG GGGCCGCTCC TTTGACAGCT TGGGGGACCT TCGGCAGGCC TTGCAGGGCT 
     2641 GCTCATTGCG AGCTGGTGAT GACTGTTTCC CGTTGCACCA CTGCTGCCTG CCCCGACCAA 
     2701 GAGAAATCTC CAACCTTGTC ATCATGCGGG GGTCTAGGGC CCACACCCGG CCACTCAACC 
     2761 TCAGTCAGCT TAGCTTCCAC AGGGTTCACC AGGATGAAAT CACCCAGCTG TCCCACTTGG 
     2821 GCCAAGGCAC AAGGACCAAT GTGTATGAGG GCCTGCTAAG TGTGGGAGGC CCCAATGAGG 
     2881 GCAAAGTGGA CAGTGGATGC CCCTCTGGGC CTGATAGGGG CAGTGGGCAG CAGCTTCGAG 
     2941 TGGTGCTCAA AGTCCTCGAC CCTAGTCACC ATGATATCGC CTTGGCCTTC TATGAGACTG 
     3001 CCAGCCTCAT GAGCCAGGTG TCACACATGC ACCTGGCTTT CCTGCATGGT GTCTGCGTGC 
     3061 GTGGCTCAGA GAATATCATT GTAGCGGAAT TCGTAGAGCA CGGTCCCCTG GATGTGTGGT 
     3121 TACGGAGACA GAGAGGCCGA GTGCCCATGC CCTGGAAGAT GGTCGTGGCT CAGCAGCTGG 
     3181 CCAGCGCCCT GAGCTACCTG GAGGACAAGA ATCTGGTTCA CGGGAACGTA TGTGGCCGGA 
     3241 ACATCCTGCT GGCCCGCCTG GGGCTGGAGG AGGGTACCAA CCCCTTCATC AAGCTCAGTG 
     3301 ACCCTGGCGT GGGCCAAGGC GCCCTCTCCA GGGAAGAGCG GGTGGAGCGA ATCCCCTGGA 
     3361 CAGCTCCGGA GTGCCTGTCC GGAGGGGCCA ACAGCTTGGG CACTGCCACG GACATGTGGG 
     3421 GCTTTGGTGC CACCCTCCTT GAGATCTGTT TTGATGGAGA GGCACCTCTG CAGGGCCGTG 
     3481 GTCCCTCCGA GAAAGAACGG TTCTACACAA AGAAACATCA GCTGCCAGAG CCCTCATGCC 
     3541 CGGAGCTGGC CACACTCACC CGTCAGTGCC TGAGCTATGA ACCCGCACAG AGGCCATCGT 
     3601 TCCGCACCAT TTTGCGGGAC CTCACAAGAC TGCAGCCACA GAATCTAGTG GGAACTTCGA 
     3661 CTGTGAACTC GGACTCACCA GCATCGGACC CCACCGTTTT TCACAAGCGT TACTTGAAAA 
     3721 GGATCCGGGA TTTGGGTGAG GGTCACTTTG GCAAGGTCAG CCTGTACTGC TATGACCCGA 
     3781 ACAATGACGG CACTGGCGAG ATGGTGGCTG TGAAAGCCCT GAAGGAGGGA TGTGGTCCCC 
     3841 AGCTCCGCTC AGGCTGGCAG CGGGAGATCG AAATCCTTCG GACGCTGTAC CACGAGCACA 
     3901 TTGTCAAGTA CAAAGGCTGC TGTGAAGACC AAGGGGAGAA GTCTGTACAG CTGGTCATGG 
     3961 AATACGTCCC TCTGGGCAGC CTCCGAGACT ACCTGCCAAG GCACAGCGTG GGGCTGGCTC 
     4021 AGCTCCTGTT GTTTGCCCAG CAGATCTGCG AGGGCATGGC TTACCTGCAC GCTCAGCACT 
     4081 ACATTCACCG AGACCTCGCT GCGCGAAACG TGCTGCTGGA CAACGACCGG CTGGTCAAGA 
     4141 TTGGAGACTT TGGCCTAGCC AAGGCTGTAC CTGAAGGCCA CGAGTACTAC CGAGTGCGCG 
     4201 AGGACGGGGA CAGCCCAGTA TTCTGGTATG CCCCAGAATG CCTAAAGGAG TGCAAGTTTT 
     4261 ACTATGCATC TGATGTCTGG TCCTTCGGGG TGACCCTTTA TGAGCTGTTG ACATACTGTG 
     4321 ACTCTAGCCA GAGTCCCCCC ACGAAATTCA TCGAGCTCAT CGGCGTAACC CAGGGCCAGA 
     4381 TGACTGTGCT GAGGCTCACA GAGCTGCTGG AGCGAGGGGA GAGGCTGCCG CGGCCTGACA 
     4441 GATGTCCCTG TGAGATCTAT CACCTCATGA AGAACTGCTG GGAGTCAGAG GCCTCCTTCC 
     4501 GGCCCACGTT CCAGAATCTT GTGCCCATCC TTAAGACAGC ACAGGAGAAG TACCAAGGCC 
     4561 AGGTGCCTTC AGTGTTCAGC GTGTGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4621 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4681 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4741 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4801 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4861 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4921 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4981 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5041 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5101 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5161 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5221 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5281 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5341 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5401 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5461 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5521 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5581 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5641 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5701 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5761 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5821 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5881 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5941 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6001 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6061 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6121 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6181 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6241 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6301 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6361 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6421 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6481 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6541 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6601 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6661 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6721 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6781 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6841 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6901 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6961 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7021 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7081 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7141 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7201 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7261 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7321 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7381 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7441 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7501 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7561 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7621 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7681 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7861 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7921 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7981 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8041 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8101 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8161 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8221 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8281 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8341 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8401 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//