LOCUS       RR217533                8439 bp    DNA     circular  SYN 
DEFINITION  Agap2 (myc-DDK-tagged) - Rat ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2)
ACCESSION   NM_023026
VERSION     NM_023026.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4596..4687 
                     /label="Myc-DDK"  
     misc_feature    4717..5302 
                     /label="PolyA signal"  
     misc_feature    4774..4793 
                     /label="Restriction Enzyme"  
     misc_feature    5578..6250 
                     /label="ColE1"  
     misc_feature    6680..7474 
                     /label="Kan/Neo"  
     misc_feature    7509..7811 
                     /label="SV40 ori"  
     misc_feature    7897..8374 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4578..4584 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4578 
                     /label="Agap2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCGGGGC GCGGGCGCGC TTCAGCGCCG GACAACGACC TACCTCATCT 
     1081 CGCTGACCCT GGTCAAGCTC GAGTCGGTAC CTCCGCCGCC GCCTTCTCCG TCTGCAGCTG 
     1141 CGGTCGGCGC CCCTGGGGCC AGAGGCTCGG AGCCCCGAGA TCCTGGCAGC CCCCGAGGCG 
     1201 CGGAGGAACC CGGCAAGAAG CGGCACGAGC GTCTCTTCCA CCGGCAGGAT GCGCTGTGGA 
     1261 TCAGTACCAG CAGCGCGGGC GCCGGGGGCG CGGAGCCCCC CGCGCTGTCC CCGGCTCCGG 
     1321 CCAGTCCGGC TCGCCCGGTC TCCCCGGCTC CGGGCCGCCG ACTGTCCCTC TGGGCCGCCC 
     1381 CTCCGGGACC CCCGCTCTCC GGGGGGCTGA GCCCGGACTC CAAGCCGGGG GGCGCCCCCT 
     1441 CTTCCTCTCG GCGCCCTCTA CTCAGCAGCC CGAGCTGGGG GGGTCCGGAA CCTGAAGGCC 
     1501 GGACGGGTGG CGGCGTCCCG GGCTCGTCCT CCCCGCATCC TGGCACCGGT AGCCGAAGGC 
     1561 TCAAGGTGGC GCCTCCTCCG CCAGCTCCCA AGCCTTTCAA GACCGTGACC ACTAGTGGAG 
     1621 CCAAAGCCGG CGGGGGCAAG GGCGCGGGTA GCCGCCTGTC ATGGCCCGAA AGCGAGGGCA 
     1681 AACCCAGGGT CAAGGGGTCA AAGAGCACCG CCGGGACTGG AGCCTCTGCC GTTGCGGCCG 
     1741 GCGGGGGAGG GAGCGCTGCA GTCACGACCT CTGGTGGGGT CGGTGCTGGG GCTGGAGCGA 
     1801 GAGGAAAATT GTCCCCTCGG AAAGGCAAGA GTAAGACCTT GGACAATAGT GACTTGCACC 
     1861 CAGGACCCAG TGCCGGTTCT CCTCCGCTCA CCGTGCCAGC AATCCCAGTT CCAGCTACTT 
     1921 CTGTCACTGC CGCCTCCACG CAGCCCCTCG GGCCTGCACC CCCTATCACT CTGGAGCCAC 
     1981 CAGCTCCGGG GCTAAAAAGG GGCCGGGAGG GCGGCCGAGC GTCCACTCGT GACCGCAAGA 
     2041 TGCTCAAGTT TATCAGCGGT ATCTTCACCA AGAGCACAGG AGGGCCTCCC GGTCCCGGGC 
     2101 CCCTTCCCGG ACCCCAAGGC TTGTCTTCCA GCAGCGGGTC CAGGGAGCTG CTGGGCGCAG 
     2161 AGCTACGCGC CTCCCCTAAG GCTGTGGTCA ATAGTCAGGA ATGGACTTTG AGCCGATCAA 
     2221 TTCCCGAACT GCGCCTGGGT GTGCTGGGTG ATGTCAGGAG TGGGAAGTCA TCCCTCATCC 
     2281 ACAGATTCCT CACGGGTTCA TACCAGGTGC TGGAGAAGCC TGAGAGTGAG CAGTACAAGA 
     2341 AGGAGATGCT GGTGGATGGG CAGACCCATC TGGTGCTGAT CCGAGAGGAA GCAGGGGCAC 
     2401 CGGATGCCAA GTTCTCAGGC TGGGCAGATG CTGTGATCTT CGTCTTCAGC CTGGAGGATG 
     2461 AGAGCAGCTT CCAGGCTGTG AGCCATCTCC ATGGGCAGCT GATCTCCCTC CGGGGGGAGG 
     2521 GACGAGGAGG TCTGGCCCTT GCCCTGGTTG GGACCCAAGA CAGGATCAGC GCATCGTCCC 
     2581 CTCGAGTGGT GGGTGATGCT CGAGCCCGGG CATTGTGTAC CGACATGAAG CGCTGCAGCT 
     2641 ACTATGAGAC CTGTGCAACC TATGGGCTCA ATGTGGACCG GGTCTTCCAG GAGGTGGCCC 
     2701 AGAAGGTGGT GACCTTGCGC AAACAGCAGC AACTCCTGGC GGCTTGCAAG TCCCTGCCCA 
     2761 GCTCACCGAG CCATTCAGCT GCTTCTACTC CTGTAGCTGG GCAGGCTAGT AATGGGGGCC 
     2821 ACACTAGCGA CTACTCTTCT TCCCTCCCAT CTTCACCCAA TGTTGGACAC CGGGAGCTCC 
     2881 GGGCTGAGGC AGCTGCGGTG GCTGGGTTGA GCACCCCAGG GTCCTTACAT CGAGCAGCCA 
     2941 AGCGGCGGAC CAGCCTTTTT GCGAACCGTC GAGGCAGTGA TTCTGAGAAA CGGAGCTTAG 
     3001 ACAGTCGGGG CGAGACAACA GGGAGTGGGC GAGCCATCCC CATTAAACAG AGTTTCCTGC 
     3061 TAAAGCGAAG TGGCAATTCC TTGAACAAAG AGTGGAAGAA GAAATACGTG ACCTTGTCCA 
     3121 GCAATGGCTT TCTGCTCTAC CACCCTAGCA TTAATGATTA CATCCACAGT ACACACGGCA 
     3181 AAGAGATGGA CTTGTTACGA ACAACAGTCA AAGTCCCAGG CAAGCGGCCC CCAAGGGCTA 
     3241 TCTCTGCTTT CGGCCCCTCA GCCAGCATCA ATGGGTTGGT CAAGGATATG AGCACTGTCC 
     3301 AGATGGGTGA AGGCCCTGAA GCCTCAACTC CAATGCCAAG CCCCAGTCCC AGCCCCAGCT 
     3361 CCCTGCAGCT CCCAACAGAC CAAACATCCA AGCACCTGTT GAAGCCAGAC CGGAATTTGG 
     3421 CCCGTGCCCT TAGCACCGAC TGTACCCCAT CTGGAGACCT GAGTCCCCTG AGTCGGGAAC 
     3481 CCCCACCTTC TCCCATGGTG AAGAAGCAGA GGAGGAAGAA ATTGTCCACA CCATCTAAGA 
     3541 CTGAAGGCTC AGCTGTGCAG GCTGAAGCCA AGCGCAAAAT GTGGAAACTA AAATCCTTTG 
     3601 GTAGTTTAAG AAATATTTAT AAAGCAGAGG AAAACTTTGA GTTCCTGATC GTGTCCAGCA 
     3661 CTGGTCAGAC GTGGCACTTT GAGGCAGCCA GCTTCGAGGA GAGGGATGCC TGGGTTCAGG 
     3721 CCATTGAGAG TCAGATCCTG GCCAGTCTGC AATGCTGTGA GAGCAGCAAG GTCAAGCTGC 
     3781 GCACTGACAG CCAAAGTGAA GCCGTGGCCA TCCAGGCGAT CCGCAACGCA AAGGGAAACT 
     3841 CGACCTGTGT GGACTGTGGA GCCCCCAACC CCACGTGGGC CAGTTTGAAT CTGGGCGCAC 
     3901 TCATCTGCAT CGAATGTTCT GGCATCCACC GGAACCTGGG CACGCACCTG TCTCGAGTGC 
     3961 GTTCGCTGGA CTTGGATGAC TGGCCGCGGG AGCTGACCCT GGTGCTGACG GCCATTGGCA 
     4021 ACGACACGGC CAATCGCGTG TGGGAGAGCG ACACTCGGGG CCGTGCCAAG CCCACGCGGG 
     4081 ACTCTTCGAG GGAGGAGCGT GAGTCGTGGA TTCGGGCCAA GTACGAACAG CTGCTGTTCC 
     4141 TGGCGCCTCT GGGCACCACG GAGGAGCCGC TGGGCCGCCA ACTGTGGGCC GCTGTGGAGG 
     4201 CCCAGGACGT GGCCGCCGTT CTGCTGCTTC TGGCCCACGC GCGACATGGG CCGCTGGACA 
     4261 CCAGCGTGGA GGACCCTCAG CTCCGCTCGC CGCTGCACTT GGCGGCCGAG CTCGCCCACG 
     4321 TTGTTATTAC ACAGCTGCTG TTGTGGTATG GCGCAGACGT GGCGGCCCGC GATGCGCAGG 
     4381 GCCGCACAGC ATTGTTCTAC GCCCGCCAGG CTGGCAGCCA GCTATGTGCA GACATCCTCC 
     4441 TCCAGCACGG CTGTCCGGGA GAGGGCGGCA GTACAGCCAC CACGCCCAGC GCGGCCACCA 
     4501 CCCCCAGCAT TACCGCCACG CCCAGCCCGA GGCGCCGGAG CAGTGCTGCC AGCTTGGGTC 
     4561 GCGTGGACAC CACGATCGCG CTGGTAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4621 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4681 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4741 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4801 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4861 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4921 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4981 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5041 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5101 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5161 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5221 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5281 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5341 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5401 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5461 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5521 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5581 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5641 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5701 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5761 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5821 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5881 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5941 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6001 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6061 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6121 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6181 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6241 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6301 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6361 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6421 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6481 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6541 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6601 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6661 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6721 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6781 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6841 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6901 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6961 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7021 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7081 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7141 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7201 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7261 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7321 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7381 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7441 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7501 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7561 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7621 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7681 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7861 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7921 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7981 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8041 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8101 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8161 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8221 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8281 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8341 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8401 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//