LOCUS       RR217531                8436 bp    DNA     circular  SYN 
DEFINITION  Ppfia4 (myc-DDK-tagged) - Rat protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4 (Ppfia4)
ACCESSION   NM_080409
VERSION     NM_080409.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4593..4684 
                     /label="Myc-DDK"  
     misc_feature    4714..5299 
                     /label="PolyA signal"  
     misc_feature    4771..4790 
                     /label="Restriction Enzyme"  
     misc_feature    5575..6247 
                     /label="ColE1"  
     misc_feature    6677..7471 
                     /label="Kan/Neo"  
     misc_feature    7506..7808 
                     /label="SV40 ori"  
     misc_feature    7894..8371 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4575..4581 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4575 
                     /label="Ppfia4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGTGAGGTG ATGCCCACAA TCAATGAGGG GGACCCTCTG GGGCCCCCCC 
     1081 ACGGTGCCGA TGCTGACGCC AACTTTGAGC AGTTGATGGT GAACATGCTG GATGAGCGAG 
     1141 AGAAGTTGCT GGAGTCCCTT CGGGAGAGTC AGGAAACTTT GGTAGCCACA CAGAGCCGGC 
     1201 TCCAAGATGC CCTTCACGAG CGAGACCAGC TCCAGCGCCA CCTGAACTCT GCCCTTCCCC 
     1261 AGGAATTTGC CACATTAACT CGGGAGCTAA GCATGTGTCG GGAGCAGCTT CTGGAGCGGG 
     1321 AGGAAGAGAT ATCTGAGCTG AAAGCAGAAC GGAATAACAC TAGGCTGCTT CTGGAACATC 
     1381 TGGAATGCCT CGTGTCCCGC CATGAGCGGT CATTGCGAAT GACAGTGGTA AAACGCCAGG 
     1441 CCCAGTCACC TTCAGGTGTC TCCAGTGAGG TGGAGGTGCT GAAGGCCCTC AAGTCACTAT 
     1501 TTGAGCACCA CAAGGCCCTG GATGAGAAGG TTCGAGAGCG GCTCCGGGCA GCTCTAGAGC 
     1561 GAGTTACCAC CTTGGAGGAG CAGTTGGCAG GAGCTCACCA GCAGGTGTCT GCCCTGCAGC 
     1621 AGGGGGCAGG AATTCGGGAT GGAGTGGCAG AAGAGGAAGA AACTGTGGAT CTGGGACCAA 
     1681 AACGCCTGTG GAAAGATGAC ACTGGCCGTG TGGAAGAACT GCAAGGGCTC TTGGAGAAGC 
     1741 AAAACTATGA GTTGAGCCAG GCCCGGGAGC GGCTGGTCAC CCTGAGTGCA ACTGTGACTG 
     1801 AGCTTGAGGA GGACCTGGGC ACAGCCCGCC GAGACCTCAT CAAGTCAGAG GAGCTGAGTG 
     1861 GCAAGCATCA GCGCGACCTC CGAGAGGCTC TGGCTCAGAA GGAGGACATG GAAGAGCGAA 
     1921 TCACCACCCT GGAGAAGCGC TACTTGGCTG CTCAGCGAGA GGCCACATCC ATCCATGACC 
     1981 TCAATGACAA ACTGGAGAAT GAGCTAGCCA ACAAGGAGTC CCTGCACCGC CAGTGTGAGG 
     2041 AGAAAGCCCG GCATCTGCAG GAGCTGTTGG AAGTGGCAGA GCAGAAGCTG CAGCAGACCA 
     2101 TGCGCAAGGC AGAGACCCTG CCAGAGGTGG AGGCCGAGCT GTCTCAGAGA ATTGCAGCTC 
     2161 TCACCAAGGC TGAGGAACGT CATGGCAACA TTGAGGAACA TCTGCGGCAG CTGGAAGGGC 
     2221 AGCTGGAGGA GAAGAACCAG GAGCTGGCAA GGGTGCGCCA GCGGGAGAAG ATGAACGAGG 
     2281 ACCACAACAA GCGGCTGTCA GACACTGTGG ACCGGTTGCT TAGTGAGTCC AACGAGCGCC 
     2341 TGCAGCTCCA CCTCAAGGAG CGAATGGCTG CCCTGGAGGA GAAGGGCCGT CTGTCTGAGG 
     2401 AGATTGAGAA GCTTCGCCAA GAGGTGGACC AGCTGAAGGG TCGAGGGGGG CCGTTTGTGG 
     2461 ATGGCATCCA CTCCAGGTCA CACGTGGGCA GTACAACAGA TGTGCGGTTC TCACTGAGTA 
     2521 CAGCCGCGCA CGTGCCTCCA GGTCTGCATC GTCGCTATAC GGCTCTGAGG GAAGAGTCTG 
     2581 CCAAGGACTG GAAGCCGGCA CCACTGCCGG GGGTGTTGGC TGCCACCACC ACGCCTGCCT 
     2641 TTGACAGTGA CCCTGAGATC TCCGATGTGG ACGAGGATGA GCCTGGTGGC TTGGTGGGCA 
     2701 CTCAGGTTGA TGTCATCTCA CCTGGTGGCC ATTCTGATGC CCAAACCCTG GCCATGATGC 
     2761 TCCAGGAACA ACTGGATGCC ATCAACCAGG AGATCAGGAT GATTCAGGAG GAGAAGGAAT 
     2821 CCACTGAGCT GCGTGCTGAA GAGATTGAGA CCCGTGTGAC CAGCGGCAGC ATGGAGGCCC 
     2881 TCAACCTGAC CCAGCTGCGC AAGCGTGGCT CCATCCCCAC CTCTCTGACC GCCCTGTCCC 
     2941 TGGCCAGTGC ATCTCCACCA CTCAGTGGCC GCTCCACACC CAAGCTCACT TCCAGAAGTG 
     3001 CTGCCCAGGA CCTGGACCGA ATGGGGGTCA TGACCCTGCC CAGCGACTTA AGAAAGCATA 
     3061 GGAGGAAGCT GCTGTCGCCA GTGTCTCGGG AAGAGAACCG AGAGGATAAA GCCACCATAA 
     3121 AATGTGAGAC TTCTCCTCCT TCCTCACCCA GGACGCTGCG GCTAGAGAAG CTTGGCCACC 
     3181 CTACCCTGAG TCAAGAAGAA GGCAAGAGTG CCTTGGAGGG CCAGGACAGC AACCCCAGCA 
     3241 GCAGCAACAG CAGCCAGGAC TCCCTGCACA AGGGCGCCAA GCGCAAGGGC ATCAAGTCGT 
     3301 CCATTGGCCG CCTGTTTGGG AAGAAGGAGA AGGGCAGGCT GATCCACTTG AGTCGGGATG 
     3361 CCACAGGCCA TGTTCTGCTC ACAGACTCCG AGCTCAGTCT GCAGGAACCA ATGGTACCTG 
     3421 CCAAATTGGG GACCCAGGCA GAGAAGGACC GGCGACTGAA AAAGAAACAC CAGCTGCTTG 
     3481 AAGATGCTCG CAGAAAAGGA ATGCCTTTTG CCCAATGGGA TGGCCCCACG GTGGTCTCCT 
     3541 GGCTGGAGCT CTGGGTGGGG ATGCCTGCTT GGTATGTGGC GGCCTGCCGG GCCAATGTCA 
     3601 AGAGTGGAGC CATCATGTCT GCCCTGTCAG ACACGGAGAT CCAGCGGGAG ATCGGCATCA 
     3661 GCAATGCCCT GCACAGGCTC AAGCTCCGCC TGGCCATCCA GGAGATGGTG TCACTGACGA 
     3721 GCCCCTCAGC CCCGCCTACC TCAAGGACAT CTTCAGGGAA TGTCTGGGTG ACCCACGAAG 
     3781 AGATGGAAAC TCTGGCAACG TCTACCAAAA CAGACAGTGA GGAGGGCAGT TGGGCCCAGA 
     3841 CCCTGGCCTA CGGAGATATG AATCACGAAT GGATTGGAAA TGAATGGCTG CCCAGCCTGG 
     3901 GGCTCCCTCA GTACCGCAGT TACTTCATGG AGTGCCTAGT GGATGCACGC ATGCTGGATC 
     3961 ACCTCACCAA GAAGGACCTG CGTGTCCACC TCAAGATGGT GGACAGTTTC CATAGAACCA 
     4021 GTCTTCAGTA TGGCATCATG TGTCTGAAGA GGCTGAACTA TGACCGGAAG GAGCTGGAGA 
     4081 AGAGGCGGGA AGAGAGCCAG CATGAGATCA AGGATGTGCT GGTCTGGACC AATGACCAGG 
     4141 TGGTTCACTG GGTCCAGTCC ATAGGGCTAA GGGATTATGC CGGAAACCTA CATGAAAGTG 
     4201 GTGTGCATGG TGCCCTGCTG GCCTTGGATG AAAACTTCGA CCACAACACA TTGGCCTTGG 
     4261 TTCTGCAGAT CCCCACACAG AACACCCAGG CCCGCCAAGT GATGGAGAGA GAGTTCAATA 
     4321 ACCTGTTGGC CTTGGGCACA GACAGGAAAT TGGATGACGG GGAAGAGAAG GTGTTCCGTC 
     4381 GAGCACCCTC CTGGAGGAAA CGCTTTCGGC CTCGGGATCA CCATAGCGGT GGCATGTTGG 
     4441 GCACCTCAGC GGAGACCCTC CCTGCGGGCT TCCGTGTGTC CACCCTGGGT CCGCTGCAGC 
     4501 CTCCACCCGC CCCGCCCAAC AAGATCATGC CGGAAGCTCA CTCCCACTAT CTCTACGGAC 
     4561 ACATGCTCTC CGCCTTCCGG GACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4621 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4681 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4741 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4801 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4861 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4921 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4981 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5041 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5101 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5161 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5221 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5281 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5341 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5401 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5461 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5521 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5581 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5641 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5701 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5761 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5821 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5881 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5941 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6001 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6061 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6121 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6181 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6241 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6301 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6361 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6421 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6481 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6541 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6601 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6661 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6721 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6781 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6841 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6901 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6961 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7021 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7081 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7141 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7201 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7261 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7321 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7381 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7441 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7501 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7561 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7621 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7681 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7801 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7861 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7921 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7981 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8041 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8101 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8161 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8221 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8281 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8341 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8401 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//