LOCUS       RR217527                8418 bp    DNA     circular  SYN 
DEFINITION  Pcdh12 (myc-DDK-tagged) - Rat protocadherin 12 (Pcdh12)
ACCESSION   NM_053944
VERSION     NM_053944.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4575..4666 
                     /label="Myc-DDK"  
     misc_feature    4696..5281 
                     /label="PolyA signal"  
     misc_feature    4753..4772 
                     /label="Restriction Enzyme"  
     misc_feature    5557..6229 
                     /label="ColE1"  
     misc_feature    6659..7453 
                     /label="Kan/Neo"  
     misc_feature    7488..7790 
                     /label="SV40 ori"  
     misc_feature    7876..8353 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4557..4563 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4557 
                     /label="Pcdh12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTACTTCTG CCTTTCCTGC TAGGGCTCTT AGGACCAGGA AGCTACTTGT 
     1081 TCATTTCAGG GGCTTGTCAG GAGGTGGCCA CTGTCACGGT GAAATTCCAA GTGGCAGAGG 
     1141 AAGTGCCATC TGGCACCGTG ATAGGGAAAC TGTCCCAAGA ACCAGGAGTG GAGGAGAGGC 
     1201 GTGGGAAGGC AGGAGATGCC TTTCAGATTC TGCAGCTGCC TCAGGCACTG CCGATTCAGA 
     1261 TGAACTCTGA GGATGGCCTG CTCAACACTT CCAGCCGGCT GGATCGGGAG AAGCTCTGTC 
     1321 GGCAGCAAGA TCCCTGCCTG GTGTCCTTTG ATGTGCTTGC CACAGGGGCT TCTGCTCTAA 
     1381 TTCATGTGGA GATTCAGGTG CTGGACATCA ATGACCACCA GCCTCAGTTT CCCAAAGATG 
     1441 AGCAGGAGCT GGAAATCTCT GAGAGTGCTT CTCTGCACAC ACGAATTCCC TTGGACAGAG 
     1501 CTCTTGACCA AGACACTGGC CCTAACAGCT TATATTCCTA TTCCCTGTCT CCCAGTGAAC 
     1561 ACTTTGCCCT GGATGTTATT GTGGGCCCTG ATGAGACCAA ACACGCAGAA CTTGTGGTGG 
     1621 TGAAGGAGTT GGACAGGGAA GTCCACTCAT ATTTTGATCT GGTGCTGACT GCCTATGACA 
     1681 ATGGGAATCC CCCCAAGTCA GGTACCAGCG TGGTCAAGGT CAATGTCCTA GATTCCAATG 
     1741 ACAATAGCCC AGTGTTTGCT GAGAGTTCAC TAGCACTAGA AATCCCAGAA GACACTGCCC 
     1801 CTGGTACTCT TCTCATAAAC CTGACTGCTA CGGATCCAGA CCAAGGCCCC AATGGGGAGG 
     1861 TAGAATTCTT CTTTGGCAAG CATGTGTCCC CAGAGGTGAT GAATACCTTT GGTATAGATG 
     1921 CTAAGACAGG CCAGATCGTT CTGCTCCAAC CCCTAGACTA CGAGAAAAAC CCTGCCTATG 
     1981 AGGTGGATGT CCAGGCAAGG GACTTAGGTC CCAATTCCAT CCCAGGCCAT TGCAAAGTTC 
     2041 TCATCAAAGT TCTGGATGTC AATGACAACG CCCCAAGCAT CCACATCACG TGGGCCTCCC 
     2101 AGATGTCGCT GGTGTCAGAA GATCTTCCCA GGGACAGCTT CATTGCTCTT GTCAGTGCGA 
     2161 ATGACTTGGA CTCAGGAAAC AATGGTCTAG TCAACTGTTG GCTGAATCAA GAGCTAGGTC 
     2221 ACTTCAGACT GAAAAGGACT AATGGCAACA CGTACATGCT GCTCACCAGT GCCATGCTGG 
     2281 ACAGAGAGCA ATGGCCCACA TACACTCTCA CTGTGTTTGC CCAAGACCAA GGACCCCAGG 
     2341 CCTTATCAGC TGAGAAGGAG CTCCAGATTC AGGTCAGTGA TGTCAATGAC AATGCCCCTG 
     2401 TATTTGAGAA GAGCCGGTAT GAGGTCTCCA CTTGGGAAAA CAACCCCCCC TCTCTTCACC 
     2461 TTATCACGCT CAAAGCTCAT GATGCTGACT TGGGCAGTAA TGGAAAAGTG TCATACCGTA 
     2521 TCAAGGACTC CCCTGTTTCT CACTTAGTCA TTATTGACTC TGAAACAGGA GAAGTCACTG 
     2581 CTCAGAGGTC GCTGGACTAT GAAGAGATGG CAGGCTTTGA GTTCCAGGTG ATAGCAGAGG 
     2641 ACAGAGGGCA ACCCCAGCTC TCATCCAGCA TCTCGGTGTG GGTTAGCCTC TTGGATGCCA 
     2701 ACGACAATAC CCCAGAAGTG ATTCAGCCTG CGCTCAGTGA AGGCAAAGCC ACCCTTTCAG 
     2761 TGCTTGTAAA TGCCTCCACG GGCCACCTTC TGTTGCCCAT TGAGAGCCCC AGTGGCATGG 
     2821 ATCCAGCAGG TACTGGTCCA ACACCACCAA AGGCTACCCA CAGCCCCTGG TCCTTCCTTT 
     2881 TGGTGACAAT CGTGGCAAGG GATGCAGACT CGGGGGCCAA TGGGGAACTC CTTTACAGCA 
     2941 TTCAAAGTGG GAATGATGCT CATCTCTTTG TCCTCAGCCC TTCCCTGGGG CAGCTATTCA 
     3001 TTAATGTCAC CAATGCCAGC AGCCTCATCG GGAGTCAATG GGACCTAGGC ATAGTGGTAG 
     3061 AGGACCAGGG GAGCCCCTCC TTGCAGACCC AAGTTTCACT GAAGGTCGTG TTTGTCACCA 
     3121 GCATGGACCA CCTAAGGGAC TCTGCTCACG AGCCCGGAAT TCTGAGCACA CCAGCGCTGG 
     3181 CTATGATCTG CCTGGCTGTA CTGCTGGCCG TCTTTGGATT GATCTTGGCT TTGCTTGTAT 
     3241 CCATCTGCCG GATAGAGAAA AGGGATAATA GGGCCTACAA CTGTCGGGAA GCTGAGTCTA 
     3301 TGTACCGCCA CCAGCCCAAG AGGCCCCAGA AACACATTCA GAAGGCAGAT ATCCACCTGG 
     3361 TGCCTATGCT TAGGGCCCAG GAGGATGAGG CTGAGGAAGT TAGGCCACCT CACAAGGATA 
     3421 CCAGTAAAGA GACATTGATG GAGGCAGGTT GGGACTCCTG CCTACAGGCC CCCTTCCACC 
     3481 TCACACCAAC CCTATACCGG ACCCTGCGTA ACCAAGGCAA CCAGGGAGCA CTGGCAGAGA 
     3541 GCCAGGAGGT ACTGCAGGAC ACCTTCAACT TTCTCTTCAA CCACCCCAGG CAGAGGAATG 
     3601 CCTCCCGAGA GAACCTCAAC CTTCCTGAGT CCCCACCCAC CCTACGCCAG CCACTCTTAA 
     3661 AGCCTCTGAA GGTGCCTGGC AGCCCCATAG CGAGGGTGAC TGGAGACCAA GGCAGCAAGG 
     3721 AGGCACCACA GAGCCCACCA GCGTCCTCTG CAACCCTAAG ACGACAGAGG AATCTCAATG 
     3781 GCAAAGTGTC TCCTAAAGGC GAGTCCAGTC CTCATCAGAT TCTCAGGAGC CTGGTTCGAC 
     3841 TCTCTGTAGC TGCTTTTGCA GAACGGAACC CTGTGGAGGA GCTTGCTGGG GACTCTCCTC 
     3901 CTGTTCAGCA AATCTCCCAG CTGCTGTCCT TGCTGCATCA GGGCCAATTC CAGCCCAAAC 
     3961 CAAACCACCG AGGAAATAAA TACTCGGCCA AGCCCGGCAG CAGCAGAGCT ACCATCCCAG 
     4021 ACACAGATGG CTTAGGCATC AGGCCTGGTG GCCTATCAGA ACCTGACTTG GAAGAAGGGC 
     4081 CCCCAAGCCC AGAGGAGGAT CTTTCTGTAA AGCGACTTCT AGAAGAAGAG TTGTCCAGCC 
     4141 TGTTGGATCC TAATACAGGT CTAGCCCTGG ACAAGCTGAG TCCCCCTGAC CCAGCCTGGA 
     4201 TGGCGAGACT GTCATTGCCC CTCACTACCA ATTACCGAGA CAACTTGTCT TCCCCTGATG 
     4261 CTGCAGCATC AGAGGAACCG AGAACCTTCC AGACATTCGG CAAGACAGTC GGACCCGGTC 
     4321 CCGAGCTAAG CGCAACAGGC ACGCGCCTGG CCAGCACTTT CGTCTCGGAG ATGAGCTCAC 
     4381 TGCTGGAGAT GTTGTTGGAG CAGAACACGG TGCCTGTGGA AGCTGCGTCT GCGGCTTTGC 
     4441 GGAGGCTCTC AGTGTGCGGG AGGACCCTGA GTCTAGACCT AGCCACCAGT GGGGCCTCGG 
     4501 GCTCAGAAGC ACAGCTGGGT AGAAAAAAGG CAGCTGAGAG CAGTCTTGGC TGTGGCAGGA 
     4561 ATCTAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4621 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4681 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4741 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4801 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4861 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4921 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4981 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5041 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5101 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5161 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5221 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5281 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5341 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5401 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5461 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5521 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5581 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5641 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5701 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5761 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5821 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5881 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5941 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6001 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6061 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6121 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6181 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6241 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6301 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6361 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6421 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6481 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6541 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6601 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6661 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6721 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6781 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6841 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6901 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6961 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7021 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7081 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7141 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7201 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7261 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7321 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7381 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7441 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7501 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7561 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7621 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7741 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7861 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7921 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7981 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8041 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8101 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8161 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8221 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8281 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8341 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8401 AAATTTAACG CGAATTTT
//