LOCUS       RR217523                8397 bp    DNA     circular  SYN 
DEFINITION  Lamb3 (myc-DDK-tagged) - Rat laminin, beta 3 (Lamb3)
ACCESSION   NM_001100841
VERSION     NM_001100841.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4554..4645 
                     /label="Myc-DDK"  
     misc_feature    4675..5260 
                     /label="PolyA signal"  
     misc_feature    4732..4751 
                     /label="Restriction Enzyme"  
     misc_feature    5536..6208 
                     /label="ColE1"  
     misc_feature    6638..7432 
                     /label="Kan/Neo"  
     misc_feature    7467..7769 
                     /label="SV40 ori"  
     misc_feature    7855..8332 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4536..4542 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4536 
                     /label="Lamb3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGACGGCA CTTTTCCTCT TGTGGCTTGC CTTGCCTGGC CTCCTGTGTG 
     1081 CCCAGCAACC CTGCTCCCGA GGGGCCTGTT ATCCACCTGT TGGGGACTTG CTCATTGGAA 
     1141 GGACTCAGCT TCTTCGAGCC TCGTCTACCT GTGGATTGAC CAAGCCTGAG ACCTATTGCA 
     1201 CCCAATATGG ACAGTGGCAG ATGAAATGCT GCAAGTGTGA CTCCAGGCTG CCTCGCAACT 
     1261 ACAACAGTCA CCGAGTGGAG AACGTTGTAT CGTCTTCAGG CCCCATGCGC TGGTGGCAGT 
     1321 CCCAGAACGA TGTGAGCCCT GTCTCTCTTC AACTGGACCT AGACAAGAGG ATGCAGCTTC 
     1381 AGGACATAAT GATGGATTTT AAGGGTCTCA TGCCAGCTGG CATGTTGATT GAGCGCTCTT 
     1441 CTGACTTTGG CAAGACATGG AGGGTGTACC AGTACCTGGC GACAGACTGT GCCAGCACCT 
     1501 TTCCCCAGGT CCACCAGGGC CAGCCCAAGA ACTGGCAGGA CGTCCGGTGC CGGCCCTTGT 
     1561 CCCAGAGGCC TAATGGGCAT CTGACTGGGG GAAAGGTCCA ACTTAACCTT ATGGATTTGG 
     1621 CATCAGCTAT CCCTGCATCT CAAAGTAAAA AGATTCAAGA ACTAGGAGAC ATCACGAACT 
     1681 TGAGAGTCAA CTTCACCAAG CTGGCCCCAG TGCCCCAGAG GGGCTCCTAT CCACCCAGCG 
     1741 CCTACTTTGC AGTATCACAG CTACGTCTAC AGGGGAGTTG CTTCTGTCAT GGACATGCCG 
     1801 ACCGCTGTGC CCCTAATCCT GGAGGCCCTA CAGGTTCCAC CACCGCTGTG CAGGTCAACG 
     1861 ATGTCTGTGT CTGCCAGCAC AACACAGCTG GCCCCAACTG TGACCGCTGC GCCCCCTTCT 
     1921 ACAACAACCG GCCATGGAGA CCTGCAGAGG GACAGGACAT CCATGAGTGC CAAAGGTGTG 
     1981 ACTGCAATGG GCACTCAGAG ACCTGCCACT TCGACCCAGC TGTGTTTGCT GCTAGCCAAG 
     2041 GGACAAGTGG AGGCGTGTGT GACAACTGCC AGGACCACAC AGAGGGCAAG AACTGTGAGC 
     2101 GATGTCAGCT ACACTATTTC CGGAATAGAC GACCAGGCGC TCCCATTCAT GAGACCTGTA 
     2161 TTCCCTGCGA GTGTGATCCA GACGGGGCAG TCCCAGGAGC TCCCTGTGAC CGACTGACGG 
     2221 GCCAGTGCGT GTGCAAAGAC CACGTGCAAG GGGAGCGCTG TGATCTTTGC AAGCCTGGTT 
     2281 TCACAGGGCT CACCTTCGCC AACCCACAGG GCTGCCATCC CTGTGACTGC AGCATCTTAG 
     2341 GTACCCGGCA AGATATGCCT TGTGAGGAGG AAACTGGACG CTGCTTGTGT CTTCCCAATG 
     2401 TAGTGGGCCC CAAATGTGAC CAGTGCGCCC CTTCCCACTG GAAGCTGGCC AGCGGCCGGG 
     2461 GCTGTGAGCC CTGTGCCTGT GACCCACGCA ACTCCCTCAG CTCCCAGTGC AACCAGTTTA 
     2521 CAGGGCAGTG TCCTTGTCGG GAAGGGTTTG GTGGCCTCAC ATGTAGTTCT GCAGCCATTC 
     2581 GTCAGTGTCC CGACCGGACC CATGGAGACG TGGCCACAGG GTGCCGAGCC TGTGACTGTG 
     2641 ACTTCAGAGG AACCGAGGGT CCTGGCTGTG ACAAAACCTC AGGCCGTTGC CTCTGCCGCC 
     2701 CCGGCTTGAC AGGGCCCCGC TGCGACCAGT GCCAACGAGG CTACTGCGAC CGCTACCCAG 
     2761 TGTGTGTGGC CTGCCACTCT TGCTTCCAGG CCTATGACAC AGACCTCCAG GAGCAGGCTT 
     2821 GGCGCCTTGG CAGTCTCCGT AATACCACTG AAGGCCTGGG CACAGGGACA GGTCTGGAGG 
     2881 ACCGTGGCCT GGCTTCTCGG TTGCTAGATG CCAAGAGCAA AATCGAGCAG ATCAGACAGA 
     2941 TTCTGGGTGG CACCTCTGTC ACAGAGCGGG ATGTGGCTCA AGTGGCCAAT GCCATCCTGT 
     3001 CTATCAGGAG GACTCTTCAG GGCCTGCCCC TGGACCTACC CTTAGAGGAG GAGATGGAAT 
     3061 CCTTCTCTGG AGACCTGGGG AATCTAGACA GAAGCTTCAA TCGCCTCCTC CTTATGTACC 
     3121 GGAGCAAGAA GGAGCAGTTT GAGAAGCTAA GCAGTGTGGA CCCTTCAGGA GCCTTCCGCA 
     3181 TGCTGACTAT GGCTTATGAG CAGTCCTCCC GGGCTGCACA GCAAGTATCC GACAGTTCCA 
     3241 GCCTGCTGAG CCAGCTGAGG GACAGTCGCA GAGAGGTAGA AGGTCTGGAG AGACAGACTG 
     3301 GAGAGGGAGG AGCTGGAGGA GCTCAGCTCA TGGCCCTGCG CCTAGAGATG GCTTCACTGC 
     3361 CTGACTTGAC ACCCACCATC AACAAGCTCT GTGGTGGCTC TAGGCAGACA GCCTGCACCC 
     3421 CAGGAGACTG CCCTGGATTG CTGTGTCCCC AGGACAATGG TACAGCCTGT GGCTCTCACT 
     3481 GCAGGGGAGC CCTGCCCAGA GCCAGAGGGG CCTTACACAT GGCAGGGCAG GTAGCTGAGC 
     3541 AGCTACGAAG CTTCAATACC CAGCTCCAGC AGACCAGGCA AATGATCAGG GCAGCTGAGG 
     3601 AGGCAGCATC AAAGGTCCAA TCAGATGCCC AGCGCCTTGA GACTCAGGTG AGTACCAGCC 
     3661 GCTTACAGAT GGAGGAAGAT GTCCGGCGCA CACGGCTTCT CATCCAGCAG GTCCGGGGCT 
     3721 TCCTCACAGA CCCTCACACA GATGCAGCCA CCATCCAACA GGTGAGCGAG GCAGTGCTGG 
     3781 CTCTCTGGCT GCCCACAGAC TCAGCTACAG TGCTACGCAA GATGAAAGAG ATCCAGGACA 
     3841 TTGCTGCCAG GCTCCCTAAC GTGGACTTAG TGCTATCCCA GACTAAGCAG GACATTGCAC 
     3901 GGGCCCGCAG GCTCCAGGCT GAGGCCGAGA AGGCCAGAAG CCGAGCCCAC GTTGTGGAAG 
     3961 GGCAGGTGGA TGACGTGGTC GGGAACCTCA GGCAGGGCAC CGTGGCTCTG CAGGAGGCTC 
     4021 AGGACACTAT GCAGGGCACT GGCCGCTCTC TTCGGCTCAT CCAGGAAAGG GTTACTGAGG 
     4081 TTCAGCAGGT CCTGGTACCA GCTGAAAGGC TGGTGAAAGG CATGAAGGAA CAGATGAGTG 
     4141 GATTCTGGGC ACGGGTGAAG GAGCTCCGCC ATCAGGCCCA GGAAGAGCAG GCACAGGCAA 
     4201 TGAAGGCCCA GCAGCTTGCA GAGGGTGCCA GCAATCAGGC CATGAATGCT CAGGAGGGCT 
     4261 TTGAGAGACT TAAGCAAAGG TATACAGAGT TGAAAGACCG GCTGGGTCAG AGTTCTATGC 
     4321 TGGGTGAGCA AGGCAATCGG ATCTTGAGCA TCAAGGCGGA GGCAGAGGAG CTCTTTGGGG 
     4381 AAACCATGGA AATGATGGAT AAAATGAAAG ACATGGAGTC AGAGCTGCTT CGAGGGAGTC 
     4441 AGGCCATCAT GCTTCGTTCA GCAGACCTGT CGGGGCTGGA GAAGCGCGTG GAGCAGATCC 
     4501 GCAGTTACAT CAACGGGCGT GTGCTGTACT ATGCCACCTG CAAGACGCGT ACGCGGCCGC 
     4561 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4621 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4681 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4741 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4801 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4861 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4921 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4981 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5041 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5101 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5161 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5221 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5281 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5341 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5401 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5461 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5521 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5581 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5641 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5701 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5761 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5821 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5881 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5941 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6001 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6061 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6121 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6181 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6241 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6301 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6361 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6421 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6481 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6541 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6601 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6661 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6721 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6781 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6841 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6901 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6961 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7021 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7081 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7141 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7201 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7261 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7321 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7381 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7441 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7501 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7561 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7621 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7801 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7861 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7921 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7981 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8041 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8101 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8161 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8221 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8281 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8341 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//