LOCUS       RR217513                8349 bp    DNA     circular  SYN 
DEFINITION  Trpm5 (myc-DDK-tagged) - Rat transient receptor potential cation channel, subfamily M, member 5 (Trpm5)
ACCESSION   NM_001191896
VERSION     NM_001191896.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4506..4597 
                     /label="Myc-DDK"  
     misc_feature    4627..5212 
                     /label="PolyA signal"  
     misc_feature    4684..4703 
                     /label="Restriction Enzyme"  
     misc_feature    5488..6160 
                     /label="ColE1"  
     misc_feature    6590..7384 
                     /label="Kan/Neo"  
     misc_feature    7419..7721 
                     /label="SV40 ori"  
     misc_feature    7807..8284 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4488..4494 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4488 
                     /label="Trpm5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGATGGCC CAGAGCTCTT GTCCTGGAAG CCCCCCAGAT ACTGGGGATG 
     1081 GATGGGAGCC AGTCCTATGC AAGGGAGAGG TCAACTTCGG AGGGTCTGGG AAAAAGCGAA 
     1141 GCAAGTTTGT GAAGGTGCCA AGCAATGTGG CCCCCTCCAT GCTCTTTGAA CTCCTGCTCA 
     1201 CCGAGTGGCA CCTGCCAGCC CCCAACCTGG TGGTGTCCCT GGTGGGCGAG GAACGGCTTT 
     1261 TTGCTATGAA GTCCTGGCTT CGGGATGTCT TGCGCAAGGG GCTGGTGAAA GCAGCTCAGA 
     1321 GCACAGGTGC CTGGATCCTG ACCAGTGCCC TCCATGTGGG CCTGGCACGC CATGTTGGAC 
     1381 AGGCTGTACG TGATCACTCT CTGGCTAGCA CGTCCACCAA GGTCCGTGTG GTGGCCATCG 
     1441 GAATGGCCTC TCTGGACCGA ATCCTTCACC GCCAACTTCT AGATGGTGTC CAGGAGGATA 
     1501 CTCCCATCCA CTACCCAGCA GATGAGGGCA GCACTCAGGG ACCCCTCTGC CCTCTGGACA 
     1561 GCAATCTCTC CCACTTCATC CTCGTGGAGC CAGGCACCCT TGGGAGTGGG AACGACGGAC 
     1621 TGGCAGAGCT GCAGCTGAGC CTGGAGAAGC ACATCTCTCA GCAGAGGACA GGTTATGGGG 
     1681 GTACCAGCAG CATCCAGATA CCTGTCCTTT GCTTGCTAGT CAATGGTGAC CCCAGCACCC 
     1741 TAGAGAGGAT GTCCAGGGCA GTGGAGCAGG CTGCCCCATG GCTGATCCTG GCAGGTTCTG 
     1801 GGGGCATTGC TGATGTACTC GCTGCCCTGG TGGGCCAGCC TCATCTCCTG GTGCCCCAGG 
     1861 TGACCGAGAA GCAGTTCAGA GAGAAATTCC CAAGCGAGTG TTTCTCTTGG GAAGCCATTG 
     1921 TACACTGGAC AGAGCTGCTA CAGAACATTG CTGCACACCC CCACCTGCTC ACAGTGTACG 
     1981 ACTTTGAGCA GGAGGGTTCC GAGGACCTGG ACACCGTCAT CCTCAAGGCA CTTGTGAAAG 
     2041 CCTGCAAGAG TCACAGCCGA GACGCACAAG ACTACCTAGA TGAGCTCAAG TTAGCAGTGG 
     2101 CCTGGGATCG CGTGGACATT GCCAAGAGTG AAATCTTCAA TGGGGACGTG GAGTGGAAGT 
     2161 CCTGTGACTT GGAAGAGGTG ATGACAGATG CCCTAGTGAG CAACAAGCCT GACTTCGTGC 
     2221 GCCTCTTTGT GGACAGTGGT GCTGACATGG CCGAGTTCTT GACCTATGGG CGGCTGCAGC 
     2281 AGCTTTACCA CTCTGTGTCC CCCAAGAGCC TCCTCTTTGA ACTGCTGGAG CGTAAGCATG 
     2341 AGGAGGGTCG GCTGACACTG GCTGGCCTGG GTGCCCAGCA GACCCGGGAG CTGCCCGTTG 
     2401 GTCTGCCTGC CTTTTCACTC CATGAGGTCT CCCGAGTTCT CAAAGATTTC CTGCATGACG 
     2461 CCTGCCGTGG CTTCTACCAG GATGGGCGCA GGATGGAGGA GAGAGGGCCA CCCAAGCGGC 
     2521 CTGCAGGCCA GAAATGGCTG CCGGACCTCA GTCGGAAGAG TGAAGACCCA TGGAGGGACC 
     2581 TGTTCCTTTG GGCTGTGCTG CAGAACCGTT ATGAGATGGC CACATACTTC TGGGCCATGG 
     2641 GCCGGGAGGG TGTGGCTGCT GCTCTGGCGG CCTGCAAGAT CATCAAGGAA ATGTCCCACC 
     2701 TGGAGAAAGA GGCAGAGGTG GCCCGCACTA TGCGTGAGGC CAAGTATGAG CAGCTGGCCC 
     2761 TCGATCTTTT CTCAGAGTGC TACAGCAACA GTGAGGACCG TGCCTTTGCC CTGTTGGTGC 
     2821 GCAGGAACCA CAGCTGGAGC AGGACCACCT GCCTGCACCT GGCCACTGAG GCCGATGCCA 
     2881 AGGCCTTCTT TGCCCATGAT GGTGTGCAAG CATTCCTGAC GAAGATCTGG TGGGGAGACA 
     2941 TGGCCACAGG CACACCCATC TTACGACTTC TGGGTGCCTT CACCTGCCCA GCCCTCATCT 
     3001 ACACAAATCT CATCTCCTTC AGTGAGGATG CCCCGCAGAG GATGGACCTG GAAGATCTGC 
     3061 AGGAGCCAGA CAGTTTGGAT ATGGAAAAGA GCTTCCTGTG CAGCCATGGT GGCCAATTGG 
     3121 AGAAGTTAAC AGAGGCGCCA AGGGCTCCTG GCGATCTAGG CCCACAAGCT GCCTTCCTGC 
     3181 TCACACGGTG GAGGAAGTTC TGGGGCGCTC CTGTGACTGT GTTCTTGGGG AATGTGGTCA 
     3241 TGTACTTTGC ATTCCTCTTC CTATTCTCCT ACGTCCTGCT GGTGGATTTC AGGCCACCAC 
     3301 CCCAGGGGCC ATCTGGGTCG GAAGTTACCC TGTATTTCTG GGTCTTCACA CTGGTGCTGG 
     3361 AGGAAATCCG ACAGGGATTC TTCACAAACG AGGACACCCG TCTGGTGAAG AAGTTCACTC 
     3421 TGTACGTAGA AGACAACTGG AACAAATGTG ACATGGTGGC CATCTTCCTG TTCATTGTTG 
     3481 GTGTCACCTG TAGAATGGTG CCCTCCGTGT TTGAGGCTGG CCGGACTGTT CTGGCCATTG 
     3541 ACTTCATGGT GTTCACACTT CGGCTCATCC ACATCTTTGC TATTCACAAG CAGCTGGGTC 
     3601 CTAAGATCAT CATTGTAGAG CGGATGATGA AAGATGTCTT CTTCTTCCTC TTCTTCCTGA 
     3661 GCGTGTGGCT CGTGGCCTAT GGCGTGACCA CTCAGGCCCT GCTGCACCCC CACGATGGCC 
     3721 GTCTGGAGTG GATTTTCCGC CGTGTGCTCT ACAGGCCTTA CCTGCAGATC TTTGGGCAAA 
     3781 TCCCTCTGGA TGAAATTGAT GAGGCCCGTG TGAACTGCTC TCTTCACCCG TTGCTGCTGG 
     3841 ACAGCTCAGC TTCCTGCCCT AATCTCTATG CCAACTGGCT GGTCATTCTC CTGCTGGTTA 
     3901 CCTTCCTCCT CGTCACTAAT GTGCTACTTA TGAACCTTCT GATCGCCATG TTCAGCTACA 
     3961 CATTCCAGGT GGTGCAGGGC AATGCAGACA TGTTCTGGAA GTTTCAACGC TACCACCTCA 
     4021 TCGTTGAATA CCACGGAAGG CCGGCTCTGG CCCCGCCCTT CATCCTGCTC AGCCACCTGA 
     4081 GCCTGGTGCT CAAGCAGGTC TTCAGGAAGG AAGCCCAGCA CAAACAGCAA CACCTGGAGA 
     4141 GAGACTTGCC TGACCCCGTG GACCAGAAGA TCATTACCTG GGAAACAGTT CAAAAGGAGA 
     4201 ACTTCCTGAG TACCATGGAG AAACGGAGGA GGGACAGTGA GGAGGAGGTG CTGAGGAAAA 
     4261 CGGCACACAG AGTGGACTTG ATTGCCAAAT ACATCGGGGG TCTGAGAGAG CAAGAAAAGA 
     4321 GGATCAAGTG TCTGGAGTCA CAGGCCAACT ACTGTATGCT CCTCTTGTCC TCCATGACTG 
     4381 ACACACTGGC TCCTGGAGGC ACCTACTCAA GTTCTCAAAA CTGTGGTCGC AGGAGTCAGC 
     4441 CAGCCTCTGC TAGAGACAGG GAGTACCTAG AGGCTGGCTT GCCACACTCA GACACCACGC 
     4501 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4561 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4621 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4681 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4741 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4801 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4861 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4921 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4981 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5041 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5101 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5161 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5221 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5281 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5341 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5401 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5461 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5521 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5581 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5641 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5701 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5761 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5821 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5881 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5941 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6001 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6061 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6121 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6181 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6241 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6301 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6361 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6421 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6481 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6541 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6601 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6661 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6721 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6781 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6841 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6901 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6961 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7021 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7081 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7141 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7201 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7261 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7321 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7381 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7441 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7501 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7561 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7801 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7861 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7921 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7981 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8041 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8101 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8161 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8221 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8281 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8341 GCGAATTTT
//