LOCUS       RR217509                8328 bp    DNA     circular  SYN 
DEFINITION  Smg7 (myc-DDK-tagged) - Rat SMG7 nonsense mediated mRNA decay factor (Smg7)
ACCESSION   NM_001191549
VERSION     NM_001191549.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4485..4576 
                     /label="Myc-DDK"  
     misc_feature    4606..5191 
                     /label="PolyA signal"  
     misc_feature    4663..4682 
                     /label="Restriction Enzyme"  
     misc_feature    5467..6139 
                     /label="ColE1"  
     misc_feature    6569..7363 
                     /label="Kan/Neo"  
     misc_feature    7398..7700 
                     /label="SV40 ori"  
     misc_feature    7786..8263 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4467..4473 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4467 
                     /label="Smg7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGACCGAA AACTTGAAAT CAGAGGAGCA TCTGAAATCA AGTAATATTA 
     1081 GGCAGGCAGA AGTCCTGAAG GCTGAAATGA CAGATTCTAA GCTGGGTCCA GCTGAGGTCT 
     1141 GGACATCCAG GCAAGCTCTG CAGGACTTGT ACCAGAAAAT GCTGGTTACT GATCTGGAGT 
     1201 ATGCTTTAGA CAAGAAGGTA GAGCAGGACC TCTGGAATCA TGCCTTTAAG AATCAGATCA 
     1261 CAACACTACA AGGCCAAGCC AAGAATCGAG CAAACCCAAA TCGGAGTGAA GTCCAGGCAA 
     1321 ACCTTTCTCT GTTCCTAGAG GCAGCTAGCG GCTTCTATAC TCAGTTATTA CAGGAACTGT 
     1381 GTACGGTGTT TAATGTAGAT TTGCCATGCC GTGTGAAGTC TTCCCAGTTG GGAATTATTA 
     1441 GCAATAAACA GACGCACACC AGCGCCATAG TGAAGCCACA GTCTAGCTCC TGTTCCTACA 
     1501 TTTGCCAGCA CTGCCTCGTC CACCTTGGAG ACATTGCTCG ATACAGAAAC CAGACTAGCC 
     1561 AGGCAGAGTC CTACTATAGG CATGCAGCTC AGCTTGTCCC CTCCAATGGT CAGCCTTACA 
     1621 ATCAGTTGGC TATCTTAGCT TCTTCCAAAG GAGACCACCT GACCACAATT TTCTACTACT 
     1681 GCAGAAGCAT TGCTGTGAAA TTCCCTTTCC CAGCTGCCTC CACTAACCTA CAAAAAGCAC 
     1741 TTTCTAAAGC ACTGGAAAGC CGGGATGAGT TGAAAACCAA GTGGGGTGTT TCTGACTTCA 
     1801 TCAAGGCCTT TATTAAATTC CACGGTCATG TATACCTGAG TAAGAGTTTG GAAAAGCTGA 
     1861 GCCCTCTTCG AGAGAAGTTA GAAGAACAGT TTAAGAGACT GCTGTTCCAA AAAGCTTTCA 
     1921 ACTCTCAGCA GTTAGTTCAT GTCACTGTCA TTAACCTGTT TCAACTTCAT CATCTTCGTG 
     1981 ACTTTAGCAA TGAAACAGAG CAGCACAGTT ACAGCCAAGA TGAACAGCTG TGTTGGACAC 
     2041 AGTTGCTGGC CCTCTTCATG TCTTTTCTTG GCATCCTGTG CAAATGTCCT CTCCAGAATG 
     2101 ATTCTCAGGA GTCCTACAAT GCCTATCCCC TTCCTGCAGT CAAGGTCTCC ATGGACTGGC 
     2161 TAAGACTCCG ACCTAGAGTC TTTCAAGAAG CTGTAGTGGA TGAAAGACAG TACATCTGGC 
     2221 CCTGGCTAAT TTCTCTTCTC AATAGTTTTC ATCCCCGTGA AGATGATCTC TCAAGTACTA 
     2281 ATGCCACACC ACTTCCAGAG GAGTTTGAAC TACAAGGGTT CCTGGCTTTG AGACCTTCTT 
     2341 TCAGGAACTT GGATTTTTCC AAAGGCCATC AGGGTATTAC AGGAGACAAA GAGGGTCAAC 
     2401 AACGGCGAAT ACGGCAGCAG CGTTTGATCT CCATAGGGAA GTGGATTGCT GACAACCAGC 
     2461 CACGGCTGAT TCAGTGTGAA AATGAGGTAG GGAAATTGTT ATTTATCACA GAAATCCCAG 
     2521 AATTAATACT AGAAGACCCC AGTGAAGCCA AAGAGAACCT CATCCTACAA GAAACATCTG 
     2581 CGATAGAGTC ACACGCTTCG GACGGGAGCC CAGGACTGAA GTCAGTGCTG TCTACAGGCC 
     2641 GAAGTCCAAG CACCAGCTGT GACTCAGGAG AGAAGCCAGT GGTCACCTTC AAAGAGAACA 
     2701 TTAAGCCACG AGAAGTGAAC AGAGACCAAG GAAGAAGCTT TCCTCCCAAA GAGGTAAAAT 
     2761 CCCAGACAGA ACTAAGAAAG ACCCCAGTGT CCGAAGCCAG GAAAACTCCT GTCACTCAAA 
     2821 CTCCAAGTCA AACAAATAAT TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC 
     2881 CTCTTCCCAG CCGACCGGGG TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT 
     2941 TTTCTATGGG CTCAGGATAC ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC 
     3001 AGTCTACAGC TCACTCTCCA GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC 
     3061 CTTACAGCCA GCAACGGCCC TCCGGACCAG GGCCAATGAA CCAGGGACCT CAACAATCAC 
     3121 AGCCACCTTC CCAGCCACCC CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA 
     3181 GCCAGTTGCA GGTTCAAGCT CTAGCTCAGC AACAGCAGTC CCCTACAAAA GTCATACCAG 
     3241 CCTTGGGGAA AAGCCCGCCT CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT 
     3301 CCAAACAGCT GTGGAATCCC CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA 
     3361 AACAGCCCTA CTACCTTCAG ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAGC 
     3421 CTCCTGTAAT ACAGCAACAG CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT 
     3481 ATAACCATAA TCCCTCAGAA GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA 
     3541 TGGCTGATAA CAGAGCAGTA ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT 
     3601 CACCCGGAGT CTTCCGTCCA GAGCAGGATC CTGTGCCCAG GATGCCGTTT GAGGACCCCA 
     3661 AGAGCTCCCC TCTGCTTCCT CCGGACCTGT TAAAGAGTCT GGCTGCCTTG GAGGAAGAGG 
     3721 AAGAGCTGAT CTTTTCTAAC CCTCCTGATC TTTACCCAGC TCTGCTGGGT CCTCTCGCCT 
     3781 CTCTTCCTGG ACGAAGCCTC TTTAAATCCT TACTGGAGAA GCCTTCCGAG CTCATGTCAC 
     3841 ATTCATCTTC TTTCCTGTCC CTCACTGGGT TCTCTGTCAA TCAGGAAAGA TATCCAAACA 
     3901 GCAGTGTGTT CAATGAAGTG TATGGGAAGA ACCTGACAGC CAGCTCCAAG GCAGAGCTGA 
     3961 GCCCCTCGGT TGCCTCCTCC CAGGAAACAT CACTGTACTC CCTCTTCGAA GGGACCCCTT 
     4021 GGTCTCCATC ACTTCCTGCC AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT 
     4081 CCTCCAACCC AAGCAGCCTG CCCAGTTCTC CTCCAACACA TAACCATAAT TCTGCTCCAT 
     4141 TCTCTAATTT TGGACCCATT GGGACTCCAG ATAACAGGGA TAGGCGGCCT GCAGATAGGT 
     4201 GGAAAACTGA TAAACCAGCC ATGGGTGGTT TCGGCGTTGA TTACCTCTCA GCAACGTCAT 
     4261 CATCTGAGAA CAGTTGGCAT CAGGCTAGCA CTCCAAGTGG CACCTGGACA GGCCACGGCC 
     4321 CCTCCATGGA GGATTCCTCC GCTGTCCTCA TGGAAAGCCT AAAGTCTATC TGGTCCAGTT 
     4381 CCATGATGCA TCCTGGACCC TCCGCTCTCG AGCAGTTGTT AATGCAGCAG AAGCAGAAAC 
     4441 AGCAGCGGGG ACAAGGTGCC ATGAACCCTC CACACACGCG TACGCGGCCG CTCGAGCAGA 
     4501 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4561 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4621 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4681 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4741 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4801 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4861 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4921 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4981 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5041 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5101 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5161 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5221 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5281 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5341 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5401 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5461 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5521 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5581 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5641 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5701 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5761 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5821 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5881 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5941 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6001 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6061 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6121 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6181 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6241 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6301 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6361 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6421 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6481 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6541 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6601 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6661 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6721 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6781 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6841 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6901 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6961 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7021 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7081 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7141 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7201 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7261 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7321 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7381 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7441 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7501 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7561 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7621 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7681 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7741 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7801 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7861 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7921 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7981 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8041 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8101 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8161 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8221 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8281 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//