LOCUS       RR217504                8316 bp    DNA     circular  SYN 
DEFINITION  Megf10 (myc-DDK-tagged) - Rat multiple EGF-like domains 10 (Megf10)
ACCESSION   NM_001100657
VERSION     NM_001100657.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4473..4564 
                     /label="Myc-DDK"  
     misc_feature    4594..5179 
                     /label="PolyA signal"  
     misc_feature    4651..4670 
                     /label="Restriction Enzyme"  
     misc_feature    5455..6127 
                     /label="ColE1"  
     misc_feature    6557..7351 
                     /label="Kan/Neo"  
     misc_feature    7386..7688 
                     /label="SV40 ori"  
     misc_feature    7774..8251 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4455..4461 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4455 
                     /label="Megf10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTATTTCT TCAAGCTCCT GCCTGGGCCT CATCTGCTCA CTGCTGTGCC 
     1081 ACTGGCTGAG GGCAGCATCC TCACTGAACC TGGAAGACCC CAATGTATGC AGCCACTGGG 
     1141 AAAGCTACTC AGTGACTGTG CAGGAGTCTT ACCCACATCC CTTCGATCAG ATTTACTACA 
     1201 CAAGCTGCAC TGACATACTG AACTGGTTTA AATGCACTCG GCACAGAGTG AGCTACCGGA 
     1261 CAGCCTACCG CCATGGGGAG AAAACCATGT ACAGACGCAA ATCCCAGTGT TGCCCCGGAT 
     1321 TTTATGAAAG TCGAGACGTG TGTGTCCCTC ACTGCGCTGA TAAATGCGTC CACGGTCGCT 
     1381 GCATCGCTCC AAACACCTGT CAGTGTGAGC CTGGCTGGGG TGGGACCAAC TGTAGCAGTG 
     1441 CTTGTGACGG TGATCACTGG GGACCTCACT GCAGCAGCCG GTGTCAGTGC AAAAACAGAG 
     1501 CTTTGTGTAA CCCCATCACC GGAGCTTGTC ACTGCGCCGC GGGCTACCGG GGATGGCGCT 
     1561 GTGAGGACCG TTGTGAACAG GGCACCTACG GTAACGACTG TCATCAAAGG TGCCAGTGTC 
     1621 AGAATGGAGC GACCTGTGAC CACATCACTG GGGAATGTCG TTGTTCACCT GGATACACTG 
     1681 GAGCCTTCTG TGAGGATCTG TGTCCTCCTG GCAAACATGG CCCACAGTGT GAGCAGAGAT 
     1741 GTCCCTGCCA AAATGGAGGC GTGTGCCATC ATGTCACTGG AGAGTGCTCC TGCCCTTCTG 
     1801 GCTGGATGGG CACAGTGTGT GGTCAGCCCT GCCCCGAGGG TCGCTTTGGA AAGAACTGTT 
     1861 CCCAAGAATG TCAGTGCCAC AATGGAGGAG CGTGTGATGC TGCCACAGGC CAATGTCACT 
     1921 GCAGCCCAGG ATACACAGGG GAACGGTGTC AGGATGAATG TCCTGTTGGA ACCTATGGAG 
     1981 TTCGCTGTGC TGAGACCTGC AGGTGTGTTA ATGGAGGGAA GTGTTACCAT GTGAGCGGCA 
     2041 CATGCCTATG CGAAGCAGGT TTTTCAGGTG AATTTTGCGA GGCTCGTCTG TGCCCTGAGG 
     2101 GGCTTTACGG CATCAAATGT GACAAGCGGT GCCCCTGCCA CCTGGACAAT ACTCATAGCT 
     2161 GTCATCCCAT GTCTGGAGAG TGTGGCTGCA AGCCGGGTTG GTCTGGACTG TACTGTAATG 
     2221 AAACATGCTC TCCTGGATTC TACGGGGAAG CTTGCCAACA GATCTGCAGC TGCCAAAACG 
     2281 GGGCTGACTG TGACAGTGTG AGTGGAAGGT GTGCCTGTGC TCCAGGATTC AAAGGAACTG 
     2341 ACTGCTCTAC TCCGTGTCCT CTGGGACGCT ATGGGATAAA TTGTTCTTCT CGCTGTGGCT 
     2401 GTAAAAACGA TGCTGTCTGT TCTCCTGTGG ATGGATCGTG CATCTGTAAG GCAGGCTGGC 
     2461 ACGGGGTGGA CTGTTCCATC AGCTGCCCCA GTGGCACATG GGGCTTTGGC TGTAATCTAA 
     2521 CCTGTCAGTG CCTCAATGGC GGTGCCTGCA ACACACTGGA CGGCACCTGC ACCTGTGCGC 
     2581 CGGGATGGCG AGGGGAGAAA TGTGAATTTC CCTGCCAGGA TGGCACATAT GGGCTGAACT 
     2641 GTGCTGAGCG CTGTGACTGC AGCCATGCAG ACGGATGTCA CCCCACTACC GGCCATTGCC 
     2701 GATGCCTCCC TGGATGGTCA GGTGTGCACT GTGACAGTGT GTGTGCTGAG GGACGCTGGG 
     2761 GTCCTAACTG CTCGCTGCCC TGCTACTGTA AAAATGGGGC TTCGTGTTCT CCCGATGATG 
     2821 GCATCTGTGA ATGCGCACCC GGATTCCGAG GCACCACTTG CCAGAGAATC TGCTCCCCGG 
     2881 GCTTTTATGG ACACCGCTGT AGCCAGACAT GCCCACAGTG TGTGCACAGC AGTGGACCAT 
     2941 GCCACCACAT CACAGGCCTG TGTGACTGCT TGCCTGGCTT CACTGGCGCC CTGTGCAATG 
     3001 AAGTGTGTCC CAGTGGCAGA TTTGGGAAGA ACTGTGCAGG TGTTTGTACC TGCACCAACA 
     3061 ACGGCACCTG TAACCCCATC GACCGGTCCT GTCAGTGCTA CCCAGGCTGG ATTGGCAGTG 
     3121 ACTGCTCCCA GCCCTGTCCA CCAGCCCACT GGGGTCCGAA CTGCATCCAT ACATGCAACT 
     3181 GCCACAATGG AGCCTTTTGC AGCGCCTATG ATGGGGAATG CAAATGCACT CCTGGCTGGA 
     3241 CGGGGCTCTA CTGCACTCAG AGATGCCCTC TGGGCTTCTA TGGCAAGGAC TGTGCACTGG 
     3301 TATGCCAATG TCAAAACGGA GCTGACTGCG ACCACATCTC GGGGCAGTGT ACCTGCCGCA 
     3361 CGGGATTCAT GGGACGGCAC TGTGAACAGA AATGCCCTGC GGGAACATAC GGCTATGGCT 
     3421 GTCGCCAGAT CTGCGACTGT CTGAACAACT CCACCTGTGA CCACATCACC GGCACGTGTT 
     3481 ACTGTAGCCC AGGGTGGAAA GGAGCACGAT GTGACCAAGC TGGGGTCATC ATCGTGGGCA 
     3541 ATCTGAACAG CTTAAGCAGG ACCAGCACCG CCCTTCCTGC TGACTCCTAT CAGATTGGGG 
     3601 CCATCGCGGG CATCGTGGTC CTTCTGCTTG TTGTGCTCTT CCTGCTGGCG CTGTTCGTTA 
     3661 TCTACAGACA CAAGCAGAAG AGGAAGGAAT CGAGCATGCC GGCCGTGACC TACACCCCTG 
     3721 CCATGAGGGT TATCAATGCA GACTATACCA TTGCAGAAAC CCTGCCTCAC AGCAATGGCG 
     3781 GAAATGCCAA CAGCCACTAC TTCACCAATC CCAGTTATCA CACACTTAGC CAGTGTGCCG 
     3841 CGTCCCCTCA TGTGAACAAC AGGGACAGGA TGACCATTGC AAAGTCAAAA AACAATCAAC 
     3901 TGTTTGTGAA TCTTAAAAAT GTGAATCCAG GGAAGAGAGG CGCTTTGGTG GACTGCACTG 
     3961 GGACGTTGCC AGCTGACTGG AAGCAAGGAG GCTACCTCAA TGAACTTGGT GCTTTTGGGC 
     4021 TGGACAGAAG CTACATGGGA AAGTCCTTAA AAGATCTGGG GAAGAATTCT GAGTACAATT 
     4081 CAAGTACTTG TTCCCTAAGC AGCTCGGAGA ACCCATATGC CACCATTAAA GACCCTCCTG 
     4141 CGCTCTTGCC TAAAAGCTCC GAGTGTGGCT ACGTGGAGAT GAAGTCGCCA GCGCGAAGAG 
     4201 ACTCCCCATA TGCAGAGATC AATAACTCAA CTTCAGCCAA CAGGAATGTC TATGAAGTCG 
     4261 AACCTACAGT CAGTGTGGTG CAAGGAGTAT TCAGCAACAA TGGTCATGTC ACCCAAGACC 
     4321 CATATGACCT TCCAAAGAAC AGTCACATCC CTTGCCATTA TGACCTGCTG CCAGTAAGGG 
     4381 ACAGTTCATC CTCCCCAAAG AGAGAGGATG GTGGTGGCAG CAGCAGCAGC ACCACCACCA 
     4441 GCAACAGCAG CAACAGCAGC GAAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4501 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4561 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4621 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4681 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4741 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4801 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4861 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4921 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4981 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5041 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5101 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5161 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5221 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5281 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5341 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5401 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5461 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5521 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5581 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5641 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5701 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5761 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5821 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5881 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5941 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6001 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6061 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6121 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6181 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6241 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6301 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6361 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6421 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6481 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6541 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6601 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6661 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6721 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6781 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6841 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6901 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6961 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7021 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7081 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7141 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7201 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7261 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7321 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7381 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7441 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7501 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7561 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7681 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7741 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7801 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7861 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7921 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7981 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8041 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8101 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8161 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8221 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8281 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//