LOCUS       RR217497                8292 bp    DNA     circular  SYN 
DEFINITION  Dusp27 (myc-DDK-tagged) - Rat dual specificity phosphatase 27 (putative) (Dusp27)
ACCESSION   NM_001191929
VERSION     NM_001191929.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4449..4540 
                     /label="Myc-DDK"  
     misc_feature    4570..5155 
                     /label="PolyA signal"  
     misc_feature    4627..4646 
                     /label="Restriction Enzyme"  
     misc_feature    5431..6103 
                     /label="ColE1"  
     misc_feature    6533..7327 
                     /label="Kan/Neo"  
     misc_feature    7362..7664 
                     /label="SV40 ori"  
     misc_feature    7750..8227 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4431..4437 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4431 
                     /label="Dusp27"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTACCAGT GGAGACACAG AGGAGGAGCA GGTAGTTCCA AACGAGGAGG 
     1081 ATGAAGCCGA CGTGAGAGCT GTACAGGCCC ACTACCTCCG GAGTCCCTCC CCAAGCCAGT 
     1141 ACTCAGTGGT CTCAGACGCA GAAACTGAAA GTATTTTCAT GGAGCCCATC CATCTCTCCT 
     1201 CGGCCGTAGC TGCCAAGCAG ATCATCAACG AAGAACTCAA GCCTCGGGGT CTCAGAACAG 
     1261 ACACGGAGTG CCCAGGCATG CTGGAGTCTG CTGAACAGCT GCTGGTGGAA GACCTGTACA 
     1321 ACCGTGTCCG GGAGAAGATG GATGACAAAA GCCTGTTCAA CACCCCCTGT GTGCTGGACC 
     1381 TGCAGCGGGC ACTGATCCAG GACCGGCAAG AGGCCCCTCG GAATGAGGTG GACGAGGTCT 
     1441 GGCCCAACGT CTTCATAGCT GAGAAGAGTG TGGCTGTGAA CAAGGGGCGA CTGAAAAGGC 
     1501 TGGGAATCAC CCACATCCTG AACGCTGCCC ATGGCACTGG CGTTTACACT GGTCCCGAAT 
     1561 TCTACACTGG CCTGGAGATC CAGTACCTGG GGGTGGAAGT GGATGATTTC CCAGAGGTGG 
     1621 ACATCTCCCA GCATTTCCGG AAGGCAGCTG AGTTTCTTGA TGAAGCCCTG CTGACCTATA 
     1681 GAGGGAAAGT CCTGGTCAGC AGTGAGATGG GTATCAGCAG GTCAGCAGTG CTGGTGGTCG 
     1741 CCTACCTGAT GATCTTCCAC AACATGGCCA TCCTGGAAGC CTTGATGACT GTCCGCCGAA 
     1801 AGCGGGCTAT CTACCCCAAC GACGGCTTCC TCAAACAGCT GCGGGAGCTC AACGAGAAAC 
     1861 TGATGGAGGA GAGGGAAGAA GAGGATGATG AAGAGGAGCC TGAGGAGGAT GCCGGGAGCA 
     1921 CACTGGGTGC TAGAGTAAAT TCCTTGATGG TGGAAGAAGA AGATGATGCC ACCAGCCACC 
     1981 TGAGTGGCTC CTCCTTGGGG AAGGCCAGCC AGGTCTCCCA GCCAGTCACC CTCATTGATG 
     2041 AAGAGGAGGA AGAGAGGCTG TATGAGGAGT GGAGGAAGGG GCAGGGCTTC CCCAAGGCTG 
     2101 AGGCTTCTCA GGGTAGAAAG GGTAGCTGTT CTGCGTCCTC CGAGCAAGAC GGGGACGACT 
     2161 GTGAGGATGG GGCTGTGGAG AGGATAATCC AGGAGTGGCA GAGCCGAAAT GAGAGGTACC 
     2221 AAGCCAAAGG GCACAAGCAG TGGAATCGGG AGGAAGACGA GGAGGAAGAG AACTCCTACA 
     2281 CCAGCAGAAG GCGCAGACAC ACCCTAAGCG AGAGCAGCGC CTCTGAGAGC GTGAGCAGCC 
     2341 ATGACATCCG GGTCCTGAAG CAGCAACTGA AGAGGACCAG CCAAAGCCGC CACGACAGAC 
     2401 ACCGCTCTGA CTCCGAGTCC ACGGAGAGCA CCTGGGACAT GTGGAACGAG AGGCTGGTGG 
     2461 AGATCGAGAA GGAGGCTGCC CGGAAGTATC GCTCCAAGAG CAAGAGGGAG GAGCTGGATG 
     2521 CCGATTCAGA GGCAGGGAGC AGGGTCCGAG AGGACGATGA GGAGAGTGTA TTGTCCGAGG 
     2581 CCAGCTCCTT CTACAACTTC TGCAGTAGGA ACAAGGACAA GCTCACTGCC CTGGAAAGGT 
     2641 GGAAGGTTAA GAGAATCCAA TTTGGATTTC ACAAGAAAGA CTCAGAGGTG GTAGACGCGG 
     2701 GCAGTGAGGA TGGTACGGAA GAGGCAACAG GAGAGAAGAA CCTTTCTGAT GTCAACCTGA 
     2761 CAGCCTACCA GGCCTGGAAG CTGAAACACC AGAAGAAGGT GGGCAATGAG AACAAGGAGG 
     2821 AGGTGGTTGA GATGAGCAAA GGAGAGGACA CAGCCTTAGC TAAGAAGAGA CAACGGAGAC 
     2881 TAGAGTTACT GGAGAGAAGC AGGCAGACGC TGGAGGAGAG CCGGTCCATG GGAAGCTGGG 
     2941 AGGGAGACAG CTCAACTGCC AGCAGGAGCA TCCCCCTGTC TGCATTCTGG TCTGCAGCCC 
     3001 CCTCTGTCAG TGTGGATGGG GACACAGCGT CAGTACTCAG CGCCCAGAGC CAGCATTCTC 
     3061 ACCTGTCTCA GCCTGCAAGT AACATGCCCA CCACACCCCT GCCTAACCTG CCAGTGGGTC 
     3121 CGGGAGACAC CATTTCCATT GCCAGTATCC AGAACTGGAT CGCCAGTGTC GTCAATGAAA 
     3181 CCCTTGCCCA AAAGCAAAAC GAAATGCTCA CGTTGTCCCG CGCCCCCTCT GTTGCAAGCA 
     3241 TGAGGGCAGC TCCAGCAGCC AGCTGCCTTG GGGATGACCA ACTCTCGGTG CTCAGCACCT 
     3301 CCCTGAGTGG CTGCCTACCG CCTCAGAGCC AGGGGAGACC CAGCTCCGAC GTGCAATCTG 
     3361 TGCTGTCCTC CACCAGCTCC CTGACCTCCA GGGCTGAAGG CAGTGAGAAC AAAGTGAGAG 
     3421 GGACCAGCAA GCCCATCTAC AGCCTCTTTG CTGACAACGT GAACCTGAAG GAGCTCGGCC 
     3481 GGAAGGAGAA AGAAATGCAG ATGGAGCTGC AGGAGAAGAT GTCCGAATAT AAAATGGAGA 
     3541 AGCTGGCCTC TGATAACAAG CGTAGCTCTC TCTTCAAGAA GAAGAAGGCC AAGGACGACG 
     3601 AGGACATCAG CGTGGGTGAC AGAGACGAGG ACACAGACAG TGCCATTGGA AGCTTCCGGT 
     3661 ATTCCTCCCG AAGTAATTCC CAGAAGCCTG AGACAGATGC CTCCTCCTCT CTGGCCATCT 
     3721 CCGATCACTT TAGAAATGGC CGCAGCGTGG GCAACGAGGT AGGTAGCAAT ATTAATACAT 
     3781 GGCTCAGTGG CCTCAGGATG GAAGAAAAAT CTCCTCCTCA AAGTGATTGG TCTGGAAGCT 
     3841 CCAGGGGGAG GTACTCCAGG TCTTCCCTGT TCCGGGAAAC AGAGTCTAAA TCCTGCAGTT 
     3901 ACAAGTTCTC CAAATCTAGG TCACAGGAAC AGGACTCCTC CTTCCACGAA ACCGATGGCA 
     3961 ACACTGTGCG AAACACCTCC CGGTTCTCCT CTTCCACCAC CAAGGAGGGC AGAGAGATGC 
     4021 ACAAGTTCTC CAGGTCCACA TTCAGCGAGA CCTCGAGCTC CCGAGAGGAG AGCCCAGAAC 
     4081 CCTATTTCTT CCGCCGAACC CCTGAACCGT CCGATGGGGA AGAGTCCCCC GAGCTAAGGC 
     4141 GTCCAAACTG GACCAGGCCC AGGGATTGGG AAGACGTAGA AGAGTCATCT AAATCAGACT 
     4201 TCGCTGAGTT TGGGGCCAAG AGGAAATTCA CCCAGAGCTT CATGAGGTCT GAAGAGGAGG 
     4261 GAGAGAAGGA GAGGACAGAA AACCGAGAGG AAGGGAGGTT TGCATCTGGG CGTCAGTCCC 
     4321 AGTATCGGAG AAGCACAAAC CGGCAAGAGG AAGAAGAAAT GGATGATGAA GCCATCATTG 
     4381 CTGCTTGGAG AAAGCGGCAA GAAGAAACCA GAACCAAACT GCAAAGAAGG AGAGAGGACA 
     4441 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4501 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4561 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4621 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4681 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4741 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4801 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4861 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4921 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4981 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5041 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5101 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5161 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5221 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5281 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5341 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5401 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5461 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5521 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5581 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5641 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5701 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5761 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5821 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5881 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5941 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6001 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6061 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6121 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6181 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6241 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6301 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6361 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6421 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6481 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6541 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6601 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6661 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6721 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6781 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6841 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6901 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6961 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7021 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7081 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7141 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7201 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7261 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7321 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7381 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7441 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7501 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7561 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7741 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7801 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7861 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7921 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7981 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8041 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8101 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8161 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8221 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8281 AACGCGAATT TT
//