LOCUS       RR217493                8283 bp    DNA     circular  SYN 
DEFINITION  Srebf1 (myc-DDK-tagged) - Rat sterol regulatory element binding transcription factor 1 (Srebf1), transcript variant 1
ACCESSION   NM_001276707
VERSION     NM_001276707.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4440..4531 
                     /label="Myc-DDK"  
     misc_feature    4561..5146 
                     /label="PolyA signal"  
     misc_feature    4618..4637 
                     /label="Restriction Enzyme"  
     misc_feature    5422..6094 
                     /label="ColE1"  
     misc_feature    6524..7318 
                     /label="Kan/Neo"  
     misc_feature    7353..7655 
                     /label="SV40 ori"  
     misc_feature    7741..8218 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4422..4428 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4422 
                     /label="Srebf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGAGCTA CCCTTCGGTG AGGCGGCTCT GGAACAGGCA CTGGCCGAGG 
     1081 TGTGCGAAAT GGACGCGGCG CTCTTGACCG ACATCGAAGA CATGCTTCAG CTCATCAACA 
     1141 ACCAAGACAG TGACTTCCCT GGCCTATTTG ATGCCCCCTA TGCTGGGGGT GAGACAGGAG 
     1201 ACACAGGCCC CAGCAGCCCT GGTGCCAGCT CTCCTGAGAG CTTCTCTTCT CCTGCTTCTC 
     1261 TGGGCTCCTC TCTGGAAGCC TTCCTGGGAG GACCCAAGGT GACACCTGCA CCCTTGTCCC 
     1321 CTCCACCATC GGCACCCACT GCTGTAAAGA TGTACCCGTC CGTGCCCCCC TTCTCCCCTG 
     1381 GGCCTGGAAT CAAAGAGGAG CCAGTGCCAC TCACCATCCT GCAGCCCCCA GCACCACAGC 
     1441 CATCGCCAGG GACCCTGTTG CCTCCGAGCT TCCCTCCTCC ACCTGTGCAG CTCAGCCCTG 
     1501 CTCCTGTGCT GGGGTACTCA AGCCTGCCTT CCGGCTTCTC AGGAACCCTT CCTGGGAACA 
     1561 CCCAGCAGAC GCCATCTAGC CTGCCACTGG GCTCCACGCC AGGAATCTCG CCCACCCCCT 
     1621 TACACACCCA GGTCCAGAGC TCGGCCGCCC AGCAGCCGCC GCCAGCCTCA GCAGCCCCTA 
     1681 GAATGAGCAC TGTGGCCTCA CAGATCCAGC AGGTCCCCGT TGTACTGCAG CCACACTTCA 
     1741 TCAAGGCAGA CTCGCTGCTG CTGACAGCTG TAAAGACAGA CACAGGAGCC ACAATGAAGA 
     1801 CCGCAGGCAT CAACACCCTG GCTCCTGGCA CAGCCGTGCA GGCAGGCCCC TTGCAGACCC 
     1861 TGGTGAGTGG AGGGACCATC CTGGCCACAG TACCACTGGT TGTGGACACA GACAAACTGC 
     1921 CCATCCACCG ACTAGCAGCT GGTGGCAAGG CCCTGGGCTC AGCTCAGAGC CGTGGTGAGA 
     1981 AGCGCACAGC CCACAATGCC ATTGAGAAGC GCTACCGTTC CTCTATCAAT GACAAGATTG 
     2041 TGGAGCTCAA GGACCTGGTG GTGGGCACTG AGGCAAAGCT GAATAAATCT GCTGTCTTGC 
     2101 GCAAGGCCAT CGACTACATC CGCTTCTTAC AGCACAGCAA CCAGAAACTC AAGCAGGAGA 
     2161 ACCTGACCCT GCGAAGTGCT CACAAAAGCA AATCACTGAA AGACCTGGTG TCAGCTTGTG 
     2221 GCAGTGGAGG AGGCACAGAT GTGTCTATGG AGGGCATGAA ACCTGAAGTG GTAGAAACGC 
     2281 TGACCCCTCC ACCCTCAGAC GCCGGCTCAC CCTCCCAGAG TAGCCCCTTG TCCTTGGGCA 
     2341 GCAGAGGCAG CAGCAGTGGT GGCAGTGACT CTGAGCCCGA CAGCCCAGCC TTTGAGGATA 
     2401 ACCAGGTGAA AGCCCAGCGG CTGCCTTCAC ATAGCCGAGG CATGCTGGAC CGCTCCCGCC 
     2461 TGGCCCTGTG TGTACTGGTC TTCCTGTGTC TGACCTGCAA CCCATTGGCC TCACTGTTTG 
     2521 GCTGGGGCAT CCTCACTCCC TCTGATGCTT CGGGTGTGCA CCGTAGTTCT GGGCGCAGCA 
     2581 TGCTGGAGGC CGAGAGCAGA GATGGCTCTA ATTGGACCCA GTGGTTGCTG CCACCCCTAG 
     2641 TCTGGCTGGC CAATGGACTA CTAGTGTTGG CCTGCTTGGC TCTTCTCTTT GTCTACGGGG 
     2701 AACCTGTGAC CAGGCCACAC TCCGGCCCGG CTGTACACTT CTGGAGACAT CGCAAACAAG 
     2761 CTGACCTGGA TTTGGCCCGG GGAGATTTTG CCCAGGCCGC TCAACAGCTG TGGCTGGCCT 
     2821 TGCAAGCCCT GGGCCGGCCC CTGCCCACCT CAAACCTGGA TCTGGCCTGC AGCCTGCTTT 
     2881 GGAACCTCGT CCGCCACCTG CTGCAGCGTC TTTGGGTGGG CCGCTGGCTG GCAGGCCAGG 
     2941 CTGGGGGCCT GCAGAGGGAC TACAGGCTGA GAAAGGATGC TCGTGCCAGT GCCCGAGATG 
     3001 CGGCTGTCGT CTACCATAAG CTGCACCAGC TGCATGCCAT GGGCAAGTAC ACAGGAGGCC 
     3061 ATCTTGTTGC TTCTAACCTG GCACTGAGTG CCCTTAACCT GGCTGAGTGT GCAGGAGATG 
     3121 CTATATCCAT GGCAACACTG GCAGAGATCT ACGTGGCAGC TGCCCTAAGG GTCAAAACCA 
     3181 GCCTCCCCAG AGCCTTGCAC TTCTTGACAC GTTTCTTCCT GAGTAGTGCC CGCCAGGCCT 
     3241 GCCTGGCACA GAGTGGTGCA GTGCCTCTTG CCATGCAGTG GCTCTGCCAC CCTGTAGGTC 
     3301 ACCGTTTCTT CGTGGATGGG GACTGGGCTG TACACGGTGC CCCCCAGGAG AGTCTGTACA 
     3361 GCGTGGCTGG GAACCCAGTG GATCCACTGG CCCAGGTGAC CCGACTATTC TGTGAACATC 
     3421 TCCTGGAGCG AGCATTGAAC TGTATCGCTC AGCCCAGCCC AGGGGCAGCT GATGGAGACA 
     3481 GGGAGTTCTC AGATGCTCTT GGATATCTAC AGTTGCTAAA TAGCTGTTCT GACGCTGTCG 
     3541 GAGCTCCTGC GTGCAGCTTC TCTGTCAGTT CCAGCATGGC TACCACCACT GGCACAGACC 
     3601 CAGTGGCCAA GTGGTGGGCC TCACTGACAG CCGTGGTGAT CCACTGGCTG AGGCGGGATG 
     3661 AGGAGGCAGC TGAACGCTTA TACCCACTGG TAGAGCACAT TCCCCAAGTG CTGCAGGAAA 
     3721 CTGAGAGACC CCTTCCCAGG GCAGCTCTGT ACTCCTTCAA GGCTGCCCGG GCTCTGCTGG 
     3781 ACCACAGAAA GGTGGAATCC AGCCCAGCCA GCCTGGCCAT CTGTGAGAAG GCCAGTGGGT 
     3841 ACCTGCGGGA CAGCTTAGCC TCTACATCAA CTGCCAGTTC CATTGACAAG GCCATGCAGC 
     3901 TGCTCCTGTG TGATCTACTT CTTGTGGCCC GCACCAGCCT ATGGCGGCGC CAACAGTCAG 
     3961 CAGCTTCAGC CCAGGGAGCT CACGGTACCA GCAATGGACC CCAGGCCTCT GCTCTGGAGC 
     4021 TGCGTGGTTT CCAACATGAC CTGAGCAGCC TGAGGCGCTT GGCACAGAGC TTCCGGCCTG 
     4081 CTATGAGGAG GGTCTTCCTA CATGAGGCCA CAGCTCGGCT GATGGCAGGA GCAAGTCCTG 
     4141 CCCGGACACA CCAGCTCCTG GACCGCAGTC TGCGGAGGCG GGCAGGTTCC AGTGGCAAAG 
     4201 GAGGCGCTGC AGCTGAGCTG GAGCCTCGAC CCACATGGCG GGAGCACACA GAGGCCTTGC 
     4261 TGTTGGCCTC CTGCTATCTG CCCCCTGCCT TCCTGTCGGC CCCCGGGCAG CGAGTGAGCA 
     4321 TGCTGGCTGA GGCAGCGCGC ACCGTGGAGA AGCTTGGCGA TCACCGGCTC CTGCTTGACT 
     4381 GCCAGCAGAT GCTCCTGCGC CTGGGTGGCG GGACCACTGT CACTTCCAGC ACGCGTACGC 
     4441 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4501 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4561 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4621 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4681 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4741 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4801 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4861 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4921 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4981 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5041 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5101 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5161 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5221 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5281 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5341 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5401 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5461 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5521 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5581 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5641 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5701 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5761 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5821 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5881 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5941 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6001 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6061 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6121 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6181 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6241 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6301 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6361 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6421 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6481 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6541 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6601 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6661 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6721 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6781 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6841 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6901 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6961 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7021 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7081 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7141 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7201 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7261 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7321 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7381 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7441 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7501 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7561 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7621 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7681 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7741 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7801 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7861 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7921 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7981 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8041 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8101 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8161 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8221 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8281 TTT
//