LOCUS       RR217492                8280 bp    DNA     circular  SYN 
DEFINITION  Polr3b (myc-DDK-tagged) - Rat polymerase (RNA) III (DNA directed) polypeptide B (Polr3b)
ACCESSION   NM_001191878
VERSION     NM_001191878.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4437..4528 
                     /label="Myc-DDK"  
     misc_feature    4558..5143 
                     /label="PolyA signal"  
     misc_feature    4615..4634 
                     /label="Restriction Enzyme"  
     misc_feature    5419..6091 
                     /label="ColE1"  
     misc_feature    6521..7315 
                     /label="Kan/Neo"  
     misc_feature    7350..7652 
                     /label="SV40 ori"  
     misc_feature    7738..8215 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4419..4425 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4419 
                     /label="Polr3b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGTGCTA GCTGAGGAGT TCGGGAGCCT GACTCCGGAG GAGCTGGCTG 
     1081 CGCCGATCCC GACTGTGGAG GAAAAATGGA GGCTGCTTCC AGCATTTTTA AAGGTAAAAG 
     1141 GCCTGGTGAA ACAGCACATC GACTCCTTTA ACTATTTCAT TAATGTAGAG ATAAAGAAGA 
     1201 TAATGAAAGC CAACGAAAAG GTTACAAGCG ATGCTGATCC CATGTGGTAC TTAAAGTATC 
     1261 TTAATATCTA TGTTGGACTT CCTGACGTCG AAGAAAGCTT CAATGTAACT AGACCAGTGT 
     1321 CCCCTCATGA GTGCCGTCTG CGGGACATGA CGTACTCCGC CCCCATCACC GTGGACATCG 
     1381 AGTATACCCG AGGCAGCCAG AGGATAATCC GCAATGCCTT ACCCATTGGC AGAATGCCCA 
     1441 TCATGCTCCG AAGTTCAAAC TGTGTTCTGA CAGGGAAAAC TCCAGCAGAG TTTGCCAAAC 
     1501 TCAACGAGTG TCCATTAGAC CCAGGTGGCT ACTTCATCGT TAAAGGAGTG GAGAAAGTCA 
     1561 TTCTTATCCA GGAGCAGCTG TCCAAGAACA GGATCATTGT GGAGGCAGAC AGGAAGGGGG 
     1621 CTGTTGGGGC TTCAGTTACC AGCTCCACCC ACGAGAAAAA AAGTAGAACC AACATGGCGG 
     1681 TGAAACAAGG GCGCTTTTAT CTGAGGCATA ACACCCTGTC TGAAGATATA CCCATTGTCA 
     1741 TCATATTCAA GGCCATGGGT GTTGAGAGTG ACCAGGAAAT TGTGCAGATG ATTGGAACGG 
     1801 AGGAGCACGT GATGGCTGCG TTTGGGCCCA GCCTGGAGGA GTGCCAGAAA GCTCAGATCT 
     1861 TCACCCAGAT GCAGGCGTTG AAGTATATAG GGAATAAAGT AAGAAGACAA AGAATGTGGG 
     1921 GAGGTGGACC AAAGAAGACC AAAATAGAAG AAGCAAGAGA ACTTCTGGCC TCTACCATTC 
     1981 TGACCCATGT ACCAGTTAAG GAATTCAATT TCCGAGCTAA GTGTATCTAC ACGGCAGTGA 
     2041 TGGTGCGAAG GGTGATCTTG GCCCAAGGAG ATAACAAGGT CGATGACAGA GACTATTACG 
     2101 GCAACAAGCG ACTGGAGCTG GCAGGCCAGC TCTTGTCTCT TCTTTTTGAA GATTTGTTTA 
     2161 AAAAGTTTAA TTCAGAAATG AAGAAGATTG CAGACCAAGT GATTCCTAAA CAAAGAGCAG 
     2221 CCCAGTTTGA CGTCGTGAAA CACATGCGCC AAGACCAGAT TACCAATGGC ATGGTCAATG 
     2281 CCATCTCCAC GGGAAATTGG TCTCTGAAGA GATTTAAGAT GGACCGGCAG GGTGTGACCC 
     2341 AAGTGCTGTC TCGCTTGTCC TATATATCTG CACTTGGCAT GATGACAAGA ATCTCTTCCC 
     2401 AGTTCGAAAA AACAAGAAAA GTGAGTGGCC CTCGTTCTCT GCAGCCATCT CAGTGGGGAA 
     2461 TGCTCTGTCC CTCCGACACA CCTGAAGGAG AGGCCTGCGG TTTGGTTAAG AACTTGGCCC 
     2521 TCATGACACA TATAACAACC GACATGGAAG ATGGACCCAT TATTAAGTTA GCTGGGAACT 
     2581 TGGGAGTAGA AGATGTGAAT TTATTATGTG GAGAAGAGCT CTCTTACCCA AATGTATTCC 
     2641 TTGTCTTCCT GAATGGTAAC ATCCTGGGTG TCATTCGAGA CCATAAAAAG CTTGTGAGCA 
     2701 CGTTCCGGCT GATGCGAAGA GCAGGCTACA TCAATGAATT TGTCTCCATC TCCACAAACC 
     2761 TTACGGACCG ATGTGTTTAC ATTTCCTCTG ATGGCGGGCG CTTGTGCAGG CCTTACATCA 
     2821 TTGTCAAGAA ACAGAAGCCC GCAGTTACCA ATAAGCACAT GGAAGAGCTG GCTCAAGGTT 
     2881 ACAGGAATTT TGAAGATTTC TTACATGAGA GTCTGGTTGA GTACTTAGAT GTGAATGAAG 
     2941 AAAATGATTG TAACATTGCG CTCTACGAAC ATACAATTAA TAAAGACACC ACCCACTTGG 
     3001 AGATTGAACC CTTCACTCTT CTCGGTGTTT GCGCCGGCCT GATCCCGTAC CCTCATCACA 
     3061 ACCAGTCTCC AAGAAACACC TACCAGTGTG CCATGGGCAA GCAAGCCATG GGTACCATTG 
     3121 GATACAATCA GCGAAACAGA ATTGATACAC TAATGTATCT ACTAGCATAT CCACAAAAAC 
     3181 CCATGGTTAA AACAAAAACC ATTGAGTTGA TAGACTTTGA GAAATTGCCA GCTGGGCAGA 
     3241 ATGCAACTGT TGCCGTGATG AGCTACAGCG GCTATGACAT TGAAGACGCC CTTGTTTTGA 
     3301 ACAAGGCCTC TCTGGACAGA GGTTTTGGGC GTTGTCTCGT CTATAAAAAC GCTAAGTGCA 
     3361 CATTGAAGCG ATATACCAAT CAGACTTTTG ATAAGGTGAT GGGTCCCATG TTGGATGCTG 
     3421 CTACCAGAAA ACCCATCTGG CGACATGAAA TTTTAGATGC AGATGGCATT TGTTCTCCAG 
     3481 GTGAGAAAGT AGAAAACAAG CAGGTGCTTG TAAACAAGTC CATGCCCACA GTGACTCAGA 
     3541 TTCCTCTGGA AGGGAGCAAC GTGCCACAGC AGCCACAGTA TAAGGATGTG CCCATCACGT 
     3601 ACAAAGGTGC AACAGACTCT TACATCGAGA AAGTGATGAT CTCTTCCAAC GCTGAGGACG 
     3661 CTTTCCTCAT CAAGATGCTC CTGAGACAGA CGCGGCGTCC AGAGATCGGA GACAAATTCA 
     3721 GCAGTCGCCA TGGACAAAAG GGTGTCTGCG GCCTGATCGT CCCCCAAGAA GACATGCCGT 
     3781 TCTGTGACTC TGGCATCTGT CCCGACATCA TAATGAACCC TCACGGCTTC CCATCGAGAA 
     3841 TGACGGTTGG AAAGTTGATT GAGTTGCTGG CCGGCAAGGC AGGCGTGTTA GACGGCAGGT 
     3901 TCCACTACGG CACCGCCTTT GGAGGGAGTA AAGTGAAGGA CGTGTGTGAA GACCTCGTTC 
     3961 GCCATGGTTA CAACTACCTG GGGAAGGACT ATGTCACCTC GGGCATCACC GGTGAGCCCT 
     4021 TAGAAGCCTA CATCTATTTT GGGCCTGTGT ACTATCAGAA ACTGAAACAC ATGGTGCTGG 
     4081 ATAAAATGCA TGCCCGGGCC AGAGGACCAC GAGCTGTCCT TACCAGGCAG CCCACTGAAG 
     4141 GGCGATCTCG CGATGGCGGC CTGCGTCTTG GAGAAATGGA GCGTGACTGT TTAATTGGCT 
     4201 ATGGAGCGAG TATGCTTTTG CTGGAGAGAC TAATGATCTC AAGTGATGCC TTCGAAGTTG 
     4261 ACGTCTGTGG GCAGTGTGGA CTACTTGGAT ATTCTGGCTG GTGTCACTAC TGCAAGTCCT 
     4321 CGTGCCACGT GTCCTCCCTC CGCATCCCAT ATGCCTGCAA GCTGCTCTTC CAGGAGTTGC 
     4381 AGTCCATGAA CATCATCCCC AGGTTGAAAC TGGCCAAGTA CAACGAGACG CGTACGCGGC 
     4441 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4501 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4561 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4621 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4681 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4741 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4801 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4861 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4921 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4981 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5041 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5101 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5161 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5221 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5281 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5341 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5401 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5461 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5521 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5581 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5641 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5701 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5761 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5821 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5881 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5941 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6001 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6061 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6121 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6181 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6241 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6301 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6361 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6421 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6481 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6541 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6601 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6661 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6721 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6781 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6841 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6901 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6961 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7021 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7081 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7141 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7201 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7261 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7321 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7381 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7441 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7501 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7561 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7621 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7681 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7741 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7801 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7861 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7921 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7981 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8041 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8101 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8161 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8221 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//