LOCUS       RR217490                8271 bp    DNA     circular  SYN 
DEFINITION  Ap4e1 (myc-DDK-tagged) - Rat adaptor-related protein complex 4, epsilon 1 subunit (Ap4e1)
ACCESSION   NM_001191742
VERSION     NM_001191742.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4428..4519 
                     /label="Myc-DDK"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    4606..4625 
                     /label="Restriction Enzyme"  
     misc_feature    5410..6082 
                     /label="ColE1"  
     misc_feature    6512..7306 
                     /label="Kan/Neo"  
     misc_feature    7341..7643 
                     /label="SV40 ori"  
     misc_feature    7729..8206 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4410..4416 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4410 
                     /label="Ap4e1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGACATG GTGGAGAGGA CGCTGACCGC ACTGCCGGGG CTCTTCTTGC 
     1081 AGAACCAGCT GGGAGGGCCT GCGGCCTCCA GAGCACCCTT CTTCTCCAGG CTGGGCGGCC 
     1141 TCATCCGTGG CGTCACTGCG CTCTCATCCA AGCACGAAGA AGAGAAGTTA ATCCAGCAGG 
     1201 AACTGAGTAG TCTGAAGGCG ACTGTTTCTG CTCCTACTAC AACACTGAAA ATGATGAAGG 
     1261 AATGTATGGT GAGACTTATA TACTGTGAGA TGCTTGGATA CGATGCTTCT TTTGGTTATA 
     1321 TACATGCAAT CAAGTTGGCC CAACAAGGAA ACCTCTTAGA AAAAAGAGTG GGTTATTTGG 
     1381 CTGTTTCTCT GTTTCTACAT GAAAGTCATG AACTTTTGCT TCTCCTTGTG AATACAGTGG 
     1441 TAAAGGACCT GCAGAGCACT AATCTGGTAG AAGTGTGTAT GGCACTCACT GTCGTCAGCC 
     1501 AGATTTTCCC TCGAGAAATG ATTCCAGCTG TCCTTCCACT AATAGAAGAG AAACTCCAAC 
     1561 ATTCTAAGGA GATTATACGA AGAAAAGCAG TTCTGGCATT ATACAAATTT TATCTCATTG 
     1621 CCCCTAATCA AGTACAGCAT ATCCATACTA AGTTTCGGAA AGCACTCTGT GACAGAGATG 
     1681 TTGGGGTCAT GGCTGCCTCT TTGCATATAT ACCTTAGGAT GATTAAGGAA AATTCTTCTG 
     1741 GATATAAAGA CTTGACTGAG AGTTTTGTAA CAATTTTAAA GCAGGTGGTG GGGGGAAAGC 
     1801 TCCCTGTAGA GTTCAGTTAC CACAGTGTGC CAGCGCCATG GTTACAGATT CAGCTCTTGA 
     1861 GAATACTGGG ACTGCTGGGA AAAGACGATG AAAGGACAAG TGAACTAATG TATGATGTTC 
     1921 TTGATGAATC CTTACGAAGA GCCGAGTTAA ATCACAATGT CACTTACGCT ATTCTGTTTG 
     1981 AGTGTGTACA CACAATCTAT TCTATTTATC CTAAATCAGA ATTACTTGAG AAGGCTGCCA 
     2041 AGTGCATTGG AAAATTTGTT CTGTCACCTA AAATAAACCT CAAATATTTA GGACTGAAGG 
     2101 CTCTTACCTA TGTTATCCAG CAGGATCCTT CTCTGGCTCT TCAACACCAG ATAACCATAA 
     2161 TTGAATGCCT AGACCACCCT GATCCCATTA TTAAAAGAGA GACTCTGGAA CTTCTTTACA 
     2221 GAATTACAAA TGCACAGAAT GTAGTAGTTA TTGTACAGAA AATGCTTGAA TATTTACATC 
     2281 AGAGCAAAGA AGAGTACATC ATCATCAACT TAGTCGGCAA AATAGCTGAA TTGGCTGAGA 
     2341 AATATGCTCC TGACAACGTG TGGTTCATTC AGACGATGAA TGCTGTGTTT TCAGTGGGAG 
     2401 GAGATGTAAT GCACCCAGAC ATTCTCAGTA ACTTTCTGAG ACTGCTAGCA GAAGGTTTTG 
     2461 ATGATGAAAC AGAAGATCAA CAGTTAAGAC TCTATGCAGT TCAGTCTTAC CTCACCTTAC 
     2521 TAGACATGGA AAACACTTTC TATCCACAGA GATTTCTTCA AGTTATGAGT TGGGTATTAG 
     2581 GAGAGTATTC CTACCTCTTA GATAGAGAAA GCCCCGAGGC AGTTATGACC AGGCTCTACA 
     2641 AGTTACTGAG GAGTGACTCC ATTTCTTCAG AAACGAAAGC ATGGTTGTTT GCTGCAGTAA 
     2701 CCAAGTTAAC ACCTCAAGCT CATTCTTCTC CCATAGTTGA GAACTTAATC CAAGAATTCA 
     2761 CTGTATCCTT GAATACTTGT CTGAGACAAC ATGCATTTGA ATTGAAACAT CTACATGAAA 
     2821 ATGAAGAGCT TATGAAGAGC TTGCTTCAGA GTGCTCAGAA TTGTGACGAC ATCATGGCAG 
     2881 ATGCTTCTTT ATCTTTTCTG GATGGTTTTG TGGCTGAAGG ACTCAGTCAG GGAGCAGCAC 
     2941 CTTATAAGCC CCGTCACCAG CGCCAAGAGG AGCAGCTCTC TCAGGAAAAA GTTCTCAATT 
     3001 TTGAACCATA TGGACTATCA TTTTCTTCAT CTGGCTTCAC TGGACGACAG TCTCCTGCTG 
     3061 GCGTTTCTCT GGGCTCAGAT ATATCTGGAA ACAGTGCTGA AACAGGGCTG AAAGAGGCAG 
     3121 GTAGCCTGAA GATGGACGGC ATAAAGAAAT TATGGGGTAA AGAAGGTTAT CTCCCCAAGA 
     3181 AGGAGAGTGG AGCTGCTGAT AAACTGGAAG CCGCACATGC TCCTGCAGAG AGTGCAATGA 
     3241 TGGAGAATGT AGACCAAGCC ATGACTAGGA AGGACCAAGC TCAAGGCCAC ACCCCTTCTA 
     3301 CAGAGGAGAA AGAAAAGCAG CTGTTGGCAT CATCGCTGTT TGTTGGGCTA GGACCAGAAA 
     3361 ATACGATCAA TTTGTTGGGA AAAGCAGATG TTGTGTCTTA CAAGTTCAGG AGGAAGTCAA 
     3421 AACCCAAGGT AGCCCAAAGT GACAAGATGC CCAGCGCTCA TGCCGCTCCC TCCTCCACCC 
     3481 TTAGCCCTGT GTCCGATGTG GCTGGGGGGG ATGAGGACAG TCTGAGCGCT GCGGATAGAG 
     3541 GAGACGGGGA GCTTGGTTCA GAGCTGTTGG GTTCTGAGTC TGTCTTGGAG CTGCCCTCAG 
     3601 TTGAAAACCT CTCGAGTGTC AGCTTGCCCG TGTCTTCTTT GTTTGCGGGT AACGACATGG 
     3661 AAGTATTTCA TCCCCCTTCA TCCTCTGCAA CTTCAGCCGT CAAGGAAGTC AGTTTGGCTG 
     3721 CTCTGTTGGA AGAAACTCCT GAATGCAGGC GTTCAGCTCT CGTGGAAATC TGTAGTAATG 
     3781 AAACTGTGGC TGTGTCTTCT TACAAAGTCT GGAAGGATGA GTGTTTATTG CTGGTGTGCT 
     3841 CAGTCACTAG TAAGACTGAT TCAGAGTTCA CAGATGCTCA ATTAGAAATT TTTCCTTTGG 
     3901 AAAATTTCAA GATCATTGAG CAGCCTGGAT GTCCTTCACC TGTGATGGAA GCAGAAAGCA 
     3961 CCAAATCCTT CCAATATGGC GTGCAGATGG AAAGCCCTTG TGTAGAGGGG ACTCTCTCTG 
     4021 GTTTTATAAA GTATCAAATG ATGGATACAC ATCCTGTTCA ACTGGAATTT TCCATGAACT 
     4081 TATCACTACT AGATTTTATT AGGCCATTAA AGATCTCAAC TGAAGACTTT GGCAAACTCT 
     4141 GGTTGTCTTT TGCAAATGAT GTGAAGCAAA ATATAAAAAT ATCAGAGCCT CGAGTTGCAC 
     4201 TGGCTTCTGT CCTGACAGAG CTGCAGCAGA AACTCAGACT TTGCGTGGTT GACGTCATAG 
     4261 GCAGTGAAGG TCTGCTGGCC TGTAAGCTGC TTCCGTCCAC CCCCTGTATC CTGCACTGCC 
     4321 GAGTACACGC TGACGTGGTA GCCCTGTGGT TCCGATCCTC TAGCTCTGTT CTTTCAGACT 
     4381 GTTTATCTTG TCACTGTCAG AAAGTGATGC AGACATCCAC GCGTACGCGG CCGCTCGAGC 
     4441 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4501 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6061 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6121 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6181 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6241 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6301 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6361 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6421 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6481 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6541 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6601 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6661 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6721 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6781 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6841 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6901 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6961 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7021 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7081 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7141 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7201 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7261 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7321 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7381 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7441 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7501 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7681 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7861 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7921 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7981 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8041 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8101 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8161 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8221 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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