LOCUS       RR217481                8211 bp    DNA     circular  SYN 
DEFINITION  Srebf1 (myc-DDK-tagged) - Rat sterol regulatory element binding transcription factor 1 (Srebf1), transcript variant 2
ACCESSION   NM_001276708
VERSION     NM_001276708.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4368..4459 
                     /label="Myc-DDK"  
     misc_feature    4489..5074 
                     /label="PolyA signal"  
     misc_feature    4546..4565 
                     /label="Restriction Enzyme"  
     misc_feature    5350..6022 
                     /label="ColE1"  
     misc_feature    6452..7246 
                     /label="Kan/Neo"  
     misc_feature    7281..7583 
                     /label="SV40 ori"  
     misc_feature    7669..8146 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4350..4356 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4350 
                     /label="Srebf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTGCACA TTTGAAGACA TGCTTCAGCT CATCAACAAC CAAGACAGTG 
     1081 ACTTCCCTGG CCTATTTGAT GCCCCCTATG CTGGGGGTGA GACAGGAGAC ACAGGCCCCA 
     1141 GCAGCCCTGG TGCCAGCTCT CCTGAGAGCT TCTCTTCTCC TGCTTCTCTG GGCTCCTCTC 
     1201 TGGAAGCCTT CCTGGGAGGA CCCAAGGTGA CACCTGCACC CTTGTCCCCT CCACCATCGG 
     1261 CACCCACTGC TGTAAAGATG TACCCGTCCG TGCCCCCCTT CTCCCCTGGG CCTGGAATCA 
     1321 AAGAGGAGCC AGTGCCACTC ACCATCCTGC AGCCCCCAGC ACCACAGCCA TCGCCAGGGA 
     1381 CCCTGTTGCC TCCGAGCTTC CCTCCTCCAC CTGTGCAGCT CAGCCCTGCT CCTGTGCTGG 
     1441 GGTACTCAAG CCTGCCTTCC GGCTTCTCAG GAACCCTTCC TGGGAACACC CAGCAGACGC 
     1501 CATCTAGCCT GCCACTGGGC TCCACGCCAG GAATCTCGCC CACCCCCTTA CACACCCAGG 
     1561 TCCAGAGCTC GGCCGCCCAG CAGCCGCCGC CAGCCTCAGC AGCCCCTAGA ATGAGCACTG 
     1621 TGGCCTCACA GATCCAGCAG GTCCCCGTTG TACTGCAGCC ACACTTCATC AAGGCAGACT 
     1681 CGCTGCTGCT GACAGCTGTA AAGACAGACA CAGGAGCCAC AATGAAGACC GCAGGCATCA 
     1741 ACACCCTGGC TCCTGGCACA GCCGTGCAGG CAGGCCCCTT GCAGACCCTG GTGAGTGGAG 
     1801 GGACCATCCT GGCCACAGTA CCACTGGTTG TGGACACAGA CAAACTGCCC ATCCACCGAC 
     1861 TAGCAGCTGG TGGCAAGGCC CTGGGCTCAG CTCAGAGCCG TGGTGAGAAG CGCACAGCCC 
     1921 ACAATGCCAT TGAGAAGCGC TACCGTTCCT CTATCAATGA CAAGATTGTG GAGCTCAAGG 
     1981 ACCTGGTGGT GGGCACTGAG GCAAAGCTGA ATAAATCTGC TGTCTTGCGC AAGGCCATCG 
     2041 ACTACATCCG CTTCTTACAG CACAGCAACC AGAAACTCAA GCAGGAGAAC CTGACCCTGC 
     2101 GAAGTGCTCA CAAAAGCAAA TCACTGAAAG ACCTGGTGTC AGCTTGTGGC AGTGGAGGAG 
     2161 GCACAGATGT GTCTATGGAG GGCATGAAAC CTGAAGTGGT AGAAACGCTG ACCCCTCCAC 
     2221 CCTCAGACGC CGGCTCACCC TCCCAGAGTA GCCCCTTGTC CTTGGGCAGC AGAGGCAGCA 
     2281 GCAGTGGTGG CAGTGACTCT GAGCCCGACA GCCCAGCCTT TGAGGATAAC CAGGTGAAAG 
     2341 CCCAGCGGCT GCCTTCACAT AGCCGAGGCA TGCTGGACCG CTCCCGCCTG GCCCTGTGTG 
     2401 TACTGGTCTT CCTGTGTCTG ACCTGCAACC CATTGGCCTC ACTGTTTGGC TGGGGCATCC 
     2461 TCACTCCCTC TGATGCTTCG GGTGTGCACC GTAGTTCTGG GCGCAGCATG CTGGAGGCCG 
     2521 AGAGCAGAGA TGGCTCTAAT TGGACCCAGT GGTTGCTGCC ACCCCTAGTC TGGCTGGCCA 
     2581 ATGGACTACT AGTGTTGGCC TGCTTGGCTC TTCTCTTTGT CTACGGGGAA CCTGTGACCA 
     2641 GGCCACACTC CGGCCCGGCT GTACACTTCT GGAGACATCG CAAACAAGCT GACCTGGATT 
     2701 TGGCCCGGGG AGATTTTGCC CAGGCCGCTC AACAGCTGTG GCTGGCCTTG CAAGCCCTGG 
     2761 GCCGGCCCCT GCCCACCTCA AACCTGGATC TGGCCTGCAG CCTGCTTTGG AACCTCGTCC 
     2821 GCCACCTGCT GCAGCGTCTT TGGGTGGGCC GCTGGCTGGC AGGCCAGGCT GGGGGCCTGC 
     2881 AGAGGGACTA CAGGCTGAGA AAGGATGCTC GTGCCAGTGC CCGAGATGCG GCTGTCGTCT 
     2941 ACCATAAGCT GCACCAGCTG CATGCCATGG GCAAGTACAC AGGAGGCCAT CTTGTTGCTT 
     3001 CTAACCTGGC ACTGAGTGCC CTTAACCTGG CTGAGTGTGC AGGAGATGCT ATATCCATGG 
     3061 CAACACTGGC AGAGATCTAC GTGGCAGCTG CCCTAAGGGT CAAAACCAGC CTCCCCAGAG 
     3121 CCTTGCACTT CTTGACACGT TTCTTCCTGA GTAGTGCCCG CCAGGCCTGC CTGGCACAGA 
     3181 GTGGTGCAGT GCCTCTTGCC ATGCAGTGGC TCTGCCACCC TGTAGGTCAC CGTTTCTTCG 
     3241 TGGATGGGGA CTGGGCTGTA CACGGTGCCC CCCAGGAGAG TCTGTACAGC GTGGCTGGGA 
     3301 ACCCAGTGGA TCCACTGGCC CAGGTGACCC GACTATTCTG TGAACATCTC CTGGAGCGAG 
     3361 CATTGAACTG TATCGCTCAG CCCAGCCCAG GGGCAGCTGA TGGAGACAGG GAGTTCTCAG 
     3421 ATGCTCTTGG ATATCTACAG TTGCTAAATA GCTGTTCTGA CGCTGTCGGA GCTCCTGCGT 
     3481 GCAGCTTCTC TGTCAGTTCC AGCATGGCTA CCACCACTGG CACAGACCCA GTGGCCAAGT 
     3541 GGTGGGCCTC ACTGACAGCC GTGGTGATCC ACTGGCTGAG GCGGGATGAG GAGGCAGCTG 
     3601 AACGCTTATA CCCACTGGTA GAGCACATTC CCCAAGTGCT GCAGGAAACT GAGAGACCCC 
     3661 TTCCCAGGGC AGCTCTGTAC TCCTTCAAGG CTGCCCGGGC TCTGCTGGAC CACAGAAAGG 
     3721 TGGAATCCAG CCCAGCCAGC CTGGCCATCT GTGAGAAGGC CAGTGGGTAC CTGCGGGACA 
     3781 GCTTAGCCTC TACATCAACT GCCAGTTCCA TTGACAAGGC CATGCAGCTG CTCCTGTGTG 
     3841 ATCTACTTCT TGTGGCCCGC ACCAGCCTAT GGCGGCGCCA ACAGTCAGCA GCTTCAGCCC 
     3901 AGGGAGCTCA CGGTACCAGC AATGGACCCC AGGCCTCTGC TCTGGAGCTG CGTGGTTTCC 
     3961 AACATGACCT GAGCAGCCTG AGGCGCTTGG CACAGAGCTT CCGGCCTGCT ATGAGGAGGG 
     4021 TCTTCCTACA TGAGGCCACA GCTCGGCTGA TGGCAGGAGC AAGTCCTGCC CGGACACACC 
     4081 AGCTCCTGGA CCGCAGTCTG CGGAGGCGGG CAGGTTCCAG TGGCAAAGGA GGCGCTGCAG 
     4141 CTGAGCTGGA GCCTCGACCC ACATGGCGGG AGCACACAGA GGCCTTGCTG TTGGCCTCCT 
     4201 GCTATCTGCC CCCTGCCTTC CTGTCGGCCC CCGGGCAGCG AGTGAGCATG CTGGCTGAGG 
     4261 CAGCGCGCAC CGTGGAGAAG CTTGGCGATC ACCGGCTCCT GCTTGACTGC CAGCAGATGC 
     4321 TCCTGCGCCT GGGTGGCGGG ACCACTGTCA CTTCCAGCAC GCGTACGCGG CCGCTCGAGC 
     4381 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4441 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4501 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4561 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4621 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4681 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4741 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4801 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4861 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4921 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4981 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5041 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5101 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5161 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5221 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5281 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5341 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5401 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5461 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5521 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5581 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5641 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5701 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5761 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5821 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5881 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5941 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6001 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6061 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6121 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6181 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6241 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6301 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6361 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6421 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6481 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6541 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6601 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6661 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6721 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6781 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6841 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6901 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6961 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7021 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7081 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7141 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7201 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7261 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7321 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7381 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7441 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7501 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7561 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7621 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7681 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7741 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7801 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7861 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7921 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7981 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8041 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8101 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8161 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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