LOCUS       RR217480                8208 bp    DNA     circular  SYN 
DEFINITION  Gli1 (myc-DDK-tagged) - Rat GLI family zinc finger 1 (Gli1)
ACCESSION   NM_001191910
VERSION     NM_001191910.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4365..4456 
                     /label="Myc-DDK"  
     misc_feature    4486..5071 
                     /label="PolyA signal"  
     misc_feature    4543..4562 
                     /label="Restriction Enzyme"  
     misc_feature    5347..6019 
                     /label="ColE1"  
     misc_feature    6449..7243 
                     /label="Kan/Neo"  
     misc_feature    7278..7580 
                     /label="SV40 ori"  
     misc_feature    7666..8143 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4347..4353 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4347 
                     /label="Gli1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCAATCCA ATGACTTCAC CACAAGTCAA TAGCTATGGT GAGCCATGCT 
     1081 GTCTCCGACC CCTCCCCAGC CAAGGGGTCC CCAGCATGGG AACAGAAGGA CTTTCTGGTC 
     1141 TGCCCTTTTG CCACCAAGCC AATTTTATGT CAGGGTCCCA GGGTTATGGG TCTGCCAGAG 
     1201 AGACCAGCAG CTGCACTGAG GGGTCACTCT TTCCTCCTCC TCGGAGTTCA GTCAAACTGA 
     1261 CTAAGAAGCG GGCTCTTTCC ATCTCGCCTC TCTCTGACGC CAGCCTCGAC CTGCAAACCG 
     1321 TAATCCGTAC CTCACCCAGC TCCCTCGTGG CTTTCATCAA CTCTCGTTGT ACATCTCCAG 
     1381 GCGGTTCCTA CGGCCATCTC TCCATTGGCA CCATGAGCCC TTCTTTAGGA TTCCCACCTC 
     1441 AGATGAGTCA CCAGAAAGGA ACTTCACCTC CCTATGGAGT CCAGCCCTGT GTTCCACATG 
     1501 ATTCTACTCA GGGTTCAATG ATGCTTCACC CCCAGTCCCG AGGACCACGT GCAACCTGCC 
     1561 AGCTGAAGTC AGAGCTGGAC ATCATGGTTG GCAAGTGCCC AGAGGAACCT TTGGAAGGGG 
     1621 ACATGTCTAG CCCCAACTCC ACGAGCACAC AGGATCACCT GCTGGGGATG CTGGATGGAC 
     1681 GGGAGGACCT GGAGAGAGAG GAGAAACCTG AGCCTGAGTC TGTGTATGAG ACGGACTGCC 
     1741 GCTGGGATGG TTGCAGCCAG GAGTTCGATT CCCAAGAACA GCTGGTGCAC CACATCAACA 
     1801 GTGAGCACAT CCACGGGGAG CGGAAGGAGT TCGTGTGCCA CTGGGGAGGC TGCTCCAGGG 
     1861 AGCTGAGGCC CTTCAAGGCC CAGTACATGC TGGTGGTGCA CATGCGCAGA CACACGGGCG 
     1921 AGAAGCCACA CAAGTGCACG TTTGAAGGCT GTCGGAAGTC CTATTCACGC CTCGAAAACC 
     1981 TGAAGACACA CCTTCGGTCG CACACCGGTG AGAAGCCTTA TATGTGTGAG CAGGAAGGCT 
     2041 GCAGCAAGGC CTTCAGCAAC GCCAGTGACC GTGCCAAGCA CCAGAATAGG ACCCACTCCA 
     2101 ATGAGAAGCC ATATGTGTGC AAACTCCCCG GCTGCACCAA GCGCTACACA GATCCCAGCT 
     2161 CGCTCCGCAA ACATGTCAAG ACTGTGCACG GTCCGGATGC CCACGTGACC AAACGACATC 
     2221 GAGGGGATGG CCCCTTACCA CGGGCACAGC CCCTCTCCAC AGTGGAGCCC AAGCGAGAAA 
     2281 GGGAAGGAGG ATCCGGCAGG GAAGAGAGCA GACTGACTGT GCCGGAGGGC ACCATGCCTC 
     2341 AGCAGAGCCC TGGAGCACAA TCCTCCTGCA GCAGTGACCA CTCCCCAGCA GGCAGCGCGG 
     2401 CCAACACGGA CAGCGGCGTG GAGATGGCCG GCAACGCAGG GGGCAGCACT GAGGACTTGT 
     2461 CCAGCTTGGA TGAAGGACCT TGCGTTGCGG CTACCGGACT CTCCACCCTT CGCCGCCTTG 
     2521 AGAACCTCAG GCTGGATCAG CTGCATCAGC TCCGGCCAAT AGGGTCCCGG GGTCTCAAAC 
     2581 TGCCCAGCTT AACCCACGCT GGCGCACCTG TGTCTCGACG TCTGGGCCCA CCGGTCTCCC 
     2641 TTGACCGCCG CAGCAGCAGC TCCAGCAGCA TTAGCTCCGC GTACACAGTC AGCCGCCGGT 
     2701 CCTCGCTGGC ATCCCCGTTC CCGCCGGGAT CCCCACCAGA GAACGGGGCG TCGTCACTAC 
     2761 CTGGCCTCAC ACCTGCCCAG CACTACATGC TCCGGGCAAG ATATGCTTCA GCCAGGGGGA 
     2821 GTGGCACCCC ACCCACTGCA GCACACAGCC TGGATCGGAT CGGGGGTCTT CCTGCACCTC 
     2881 CTTGGAGAAG CCGAGCTGAG TACCCAGGAT ACAACCCCAA TGCAGGGGTC ACGAGGAGGG 
     2941 CCAGTGACCC AGCCCGGGCT GCTGACCACC CGGCTCCAGC TAGAGTACAA CGGTTCAAGA 
     3001 GCTTGGGGTG TGTACACACG CCTCCTAGTG TGGCAACAGG ACGGAACTTC GATCCCCACC 
     3061 TCCCTACCTC TGTCTATTCG CCACAGCCCC CCAGCATCAC CGAAAATGTT GCTATGGATA 
     3121 CTAGAGGGCT ACAGGGGGAG CTAGAGGTTG GAACTTCCAT GATGGGCAAT GATCTGAACC 
     3181 CATACATGGA CTTTTCTTCT GCTGACACTC TGGGATATGG GGGGCCTGAG GGGACGGCGG 
     3241 CTGAGTCTTA TGGATCTAAG GGTCCAGGTT CCCTGCCTCT TGGGCCTGGT CCACCAACCA 
     3301 ACTATGGCCC TGGCCACTGT GCCCAGCAGG TCTCCTATCC TGATCCCACC CCAGAAACAT 
     3361 GGGGTGAGTT TCCTTCACAT GCTGGGGTGT ACCCTGGCAC TAAGGCTCTA GGTACAGCCT 
     3421 ATAACCAGTG TCCTCGACTT GAGCATTATG GACAAGTGCA AGTAAAACCA GAACAAGGGT 
     3481 GCCCAGTGGG GTCTGACTCC ACCGGGTTGG CACCCTGCCT CAATGCCCAC CCCAGTGAAG 
     3541 GGTCCCCAGA CCCACAGCCT CTGTTTTCAC ATCACTCCCA GCTCCCTCCA CCCCAGTATC 
     3601 TCCAGTCAGG TCCCTACCCT CAGCCTCCCC ATGACTGTCT CTCGTTAGAA TCCAGGCCTG 
     3661 GCCTCAATTT CAACCCCTCC TCTCATTCCA CAGGACAGCT CAAAGCTCAG CTCCTGTGTA 
     3721 ATTACGTTCA GTCGCAGCAG GAATTGTTGT GGGAGGGCAG AAGCCGGGGA GGGATCCCCA 
     3781 ACCAGGAACT TCCATATCAG AGCCCCAAGT TTCTGGGAGG TTCTCAAGTT AGTCAGAGCC 
     3841 CCACCAAGGT CCCAGCAGCA GCAGCAGCAG CTACGTTTGG ATCTGGCTTT GCATCACCTG 
     3901 CTTTGGCCAA TCACAAATCA GTCTCCTATC CTGCCCCTTC ACCGTGCCAT GAAACTTTCA 
     3961 CCATGGGAGT AAACAGGCCT CCCCATAGGC CAGCAGCACC ACCCCGGCTT CTGCCCCCGC 
     4021 TACCACCTTG CTATGGGCCC CTCAAGGTGG GAGATGCCAA CCCCAGCTGT GGGCATCCTG 
     4081 AAGTGGGCAG GTTAGGAGCA GGCCCTGCCT TGTACCCTCC CCCTGAAGGG CAGGTGTGTA 
     4141 ACCCTCTGGA CTCTCTTGAC CTGGACAACA CTCAGCTGGA CTTTGTGGCT ATCCTAGATG 
     4201 AAGCTCAAGG GCTGAGCCCT CCTCCTTCCC ACGAGCAAGG GGACAGCTCT AAAAACACCC 
     4261 CACCTCCCTC CGGGCCCCCC AACATGGCAG TCGGTAACAT GAGTGTCTTA CTGGGGTCTC 
     4321 TGCCTGGAGA GACGCAGTTC CTCAACTCTA GTGCCACGCG TACGCGGCCG CTCGAGCAGA 
     4381 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4441 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4501 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4561 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4621 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4681 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4741 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4801 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4861 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4921 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4981 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5041 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5101 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5161 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5221 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5281 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5341 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5401 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5461 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5521 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5581 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5641 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5701 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5761 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5821 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5881 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5941 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6001 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6061 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6121 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6181 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6241 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6301 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6361 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6421 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6481 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6541 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6601 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6661 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6721 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6781 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6841 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6901 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6961 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7021 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7081 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7141 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7201 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7261 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7321 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7381 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7441 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7501 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7561 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7621 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7681 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7741 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7801 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7861 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7921 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7981 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8041 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8101 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8161 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//