LOCUS       RR217478                8205 bp    DNA     circular  SYN 
DEFINITION  Otud4 (myc-DDK-tagged) - Rat OTU deubiquitinase 4 (Otud4)
ACCESSION   NM_001191700
VERSION     NM_001191700.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4360..4451 
                     /label="Myc-DDK"  
     misc_feature    4481..5066 
                     /label="PolyA signal"  
     misc_feature    4538..4557 
                     /label="Restriction Enzyme"  
     misc_feature    5342..6014 
                     /label="ColE1"  
     misc_feature    6444..7238 
                     /label="Kan/Neo"  
     misc_feature    7273..7575 
                     /label="SV40 ori"  
     misc_feature    7661..8138 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4335..4342 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4335 
                     /label="Otud4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCAGCT GTCGGCGCGC CCGACGGCGT GGACCAGGGC GGCGCGGGAC 
     1081 CGCTGGAGGA TGCGACGCCC ATGGACGCCT ATCTGCGCAA GCTGGGATTG TATCGGAAAT 
     1141 TGGTCGCCAA GGACGGGTCG TGCTTGTTCC GGGCTGTGGC CGAGCAGGTA TTACACTCTC 
     1201 AGTCTCGACA TGTGGAAGTT AGAATGGCTT GTATTCGCTA CCTTCGAGAT AACAGAGAGA 
     1261 AATTTGAAGC GTTTATAGAA GGGTCATTTG AAGAATATTT AAAACGTTTG GAAAACCCAC 
     1321 AGGAATGGGT AGGACAAGTG GAGATAAGCG CCCTCTCTCT TATGTACAGG AAAGATTTTG 
     1381 TAATATATCA GGAGCCAAAT GTTTCTCCTT CGCATGTAAC TGAAAATAAT TTTCCTGAGA 
     1441 AGGTGTTACT GTGCTTTTCG AATGGAAATC ATTATGACAT TGTCTATCCT ATAACATACA 
     1501 GAGATAGTTC TGCTGTGTGT CAGTCTCTCC TTTATGAATT GCTGTATGAG AAGGTATTTA 
     1561 AAACTGACGT CAGTAAAATC ATGATGGGAC TAGAAACTTC TGAAGTGGCT GATGAAAGCA 
     1621 ACAGTGAAAT ATCAGATTCT GAGGATGACA GTTGCAAGAG TAAAAGCACT GCTGCTACTG 
     1681 ATGTGAATGG ATTTAAGTCC TCAGGCAGTG AGAACCCTAA GAACCATGGG AACTCAGCTG 
     1741 ACCTTCCTTT GTCCAGAAAG GTTCTTAAGT CCCTCAGCCC AGCAGTCTAT AGAAATGTGG 
     1801 AGTATGAAAT TTGGTTGAAG TCTAAGCAAG CTCAACAAAA ACGTGATTAT TCCATTGCTG 
     1861 CTGGCTTACA ATATGAAGGA GGAGAGAGAT GCCATGTTAG ATTGGATCAT AATGGGAAGT 
     1921 TATCTAATGC AGACATTCAT GGGGTTCACT CTGAGAATGG ACCGGTTTTG TCTGAAGAAC 
     1981 TGGGGAAAAA GCAAACACCG AAGAACCTCA AGCCACCTCC CCCAGAAAGC TGGAACACGG 
     2041 TGTCCGGAAA GAAGATGAAA AAACCTAATT CTGGGCAAAA TTTCCATTCA GATACGGATT 
     2101 ACAGAGGGCC AAAGAATCTA AACAAGCCAA TCAAAGCCCC GTCTGCACTA CCTCCTCGCC 
     2161 TGCAGCATCC TCCAGCGGGT GTAAGGCAGC ACGCGCTCTC CAGTCATTCT ACGGGGCCCC 
     2221 AGTCTCAGAA ATCCTCCAGT GAGCATAAGA ATCTAAGTCG GGTGCCATCA CAGATCACAA 
     2281 GAAAACCTGA TCGTGAAAGA GCTGAGGACT TTGATCATGT GAGTCGTGAA TCTTACTATT 
     2341 TTGGCCTCTC GCCAGAAGAA CGCAGAGAGA AGCAAGCTAT TGAAGAGTCT CGGCTACTCT 
     2401 ATGAGATTCA GAACCGGGAT GAACAGGCTT TTCCGGCTCT TTCTAGTTCA TCAGTCAGTC 
     2461 AGTCACCTCA GAATAGCAAC GCGTGCGTCC CAAGGAAGTC TTCACATGCG AGGGACAGGA 
     2521 AAGGAAGCAT GCGGAGAGTA GATGCAGAGG AACGGAAGGA CAAAGACTCT CTACGTGGCC 
     2581 ATACTCACAT GGATAAAAAA CCCGAGCCAA GCACACTGGA GATCAGCGAT GATAAATGTA 
     2641 CAAGAGTTTC ATCACCATCT AAGTCAAAGA AAGAGTGCCC ATCTCCTGTA GAACAAAAGC 
     2701 CAGCAGAACA TATACCTTTG TCAAATCCAG CCCCCCTCCT AGTCTCTCCA GAAGTTCATC 
     2761 TCACTCCTGC GGTGCCTTCT TTACCAGCCA CTGTGCCAGC CTGGCCAAGT GAACCCACAA 
     2821 CTTTCGGACC AACAGGTGTC CCTGCTCAGA TTCCCATTTT GTCAGTGACA CAGACCACTG 
     2881 GACCTGATGC TGCCGTGTCA CAAGCGCATT TAACACCCTC TCCGGTTCCT GTGTCAATTC 
     2941 AGGCAGTTAA CCAGCCCTTG ATGCCTTTGC CTCAGACAAT GAGCCTCTAT CAAGACCCAC 
     3001 TCTATCCTGG GTTTCCTTGT AGTGAGAAGG GAGATCGAGC CATTGCACCA CCCTATTCAC 
     3061 TGTGTCAGAC CGGCGAGGAC CTGCCTAAAG ATAAGAATAT TCTTCGATTC TTCTTCAATC 
     3121 TTGGTGTAAA GGCATATAGT TGCCCTATGT GGGCCCCACA CTCTTACCTG TATCCTCTGC 
     3181 ACCAGGCGTA CATGGCAGCC TGCAGGGTGT ACCCAAAGGT CCCTGTTCCT GTGTATCCTC 
     3241 AGAGTACTTG GTTCCAAGAA GCCCCTCCTG CTCAGAGTGA AAGTGACTGC CCCTGTGCTG 
     3301 ACGCCCACTA CTCTATGCAC CCTGAGGCCA GTGTTAATGG CCAGATGCCA CAGGCAGAGA 
     3361 TCGGACCACC TGCGTTTTCT TCCCCTCTGG TTATCCCTCC ATCTCAGGTG TCTGAAGGTC 
     3421 ATGGACAGTT GTCTTACCAA CCTGAACTGG AGTCTGAGAG CCCAGGGCAG CTTCTTCACG 
     3481 CTGAGTATGA AGAGTCACTT AGCGGCAAGA ACATGTACCC ACAGCAGTCC TTTGGGCCTA 
     3541 ATCCATTCTT AGGTCCTGTT CCCATTGCAC CTCCTTTCTT CCCTCATGTT TGGTATGGGT 
     3601 ATCCTTTTCA GGGATTTGTG GAAAATCCTG TAATGAGGCA AAATATTGTC CTGCCCCCTG 
     3661 ATGATAAAGG AGAGTTGGAT TTGCCTTTGG AAAATCTAGA TCTGTCTAAA GAGTGCGATT 
     3721 CTGTCTCAGC AGTAGACGAG TTCCCAGAAG CCAGAGTTGA AGGCGCACAT TCTCTCTCTG 
     3781 AAGCAAGTGT GAGCAGCAAG CATGAAGGCC GGGTGGAGCA GTCGTCCCAG ACGCGGAAGG 
     3841 CAGACATAGA CTTGTCTTCA GGTTCTTCTG GAGTGGAGGG AAAGGCTCAC CCCCCTACTC 
     3901 AGATTCTAAA CCGAGAGAGA GAGGCTGTGT CTGCTGAACC TGAGCCTAAG AGGACCATTC 
     3961 AAAGTCTGAA AGAAAAACCA GAGAAAGTCA AAGACCCCAA GACTGCTGCC GATGTGCTCA 
     4021 GCTCTGGGGC CAACTCTGTG GATAGATTGC AAAGACCAAA AGAAGAGAGT TCAGAAGATG 
     4081 AGAACGAAGT GTCTAATATT TTGAGAAGTG GCAGATCCAA GCAGTTTTAT AATCAAACTT 
     4141 ATGGAAGCAG GAAGTACAAA AGTGATTGGG GCTCTTCTGG TAGAGGTGGC TATCAACATG 
     4201 TGAGAGGCGA GGAGTCCTGG AAAGGGCAGC CTAACAGAAG CCGGGATGAA GGTTATCAGT 
     4261 ACCATCGACA TGTTAGAGGA CGCCCTTACA GGGGAGATAG GAGGAGGTCA GGGATGGGAG 
     4321 ATGGCCACAG GGGACAACAC ACTAGCGGAC CGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4381 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4441 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4501 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4561 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4621 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4681 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4741 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4801 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4861 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4921 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4981 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5041 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5101 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5161 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5221 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5281 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5341 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5401 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5461 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5521 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5581 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5641 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5701 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5761 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5821 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5881 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5941 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6001 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6061 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6121 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6181 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6241 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6301 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6361 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6421 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6481 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6541 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6601 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6661 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6721 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6781 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6841 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6901 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6961 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7021 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7081 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7141 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7201 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7261 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7321 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7381 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7441 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7501 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7621 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7681 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7741 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7801 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7861 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7921 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7981 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8041 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8101 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8161 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//