LOCUS       RR217447                8070 bp    DNA     circular  SYN 
DEFINITION  Pdzrn3 (myc-DDK-tagged) - Rat PDZ domain containing RING finger 3 (Pdzrn3)
ACCESSION   NM_001271251
VERSION     NM_001271251.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4227..4318 
                     /label="Myc-DDK"  
     misc_feature    4348..4933 
                     /label="PolyA signal"  
     misc_feature    4405..4424 
                     /label="Restriction Enzyme"  
     misc_feature    5209..5881 
                     /label="ColE1"  
     misc_feature    6311..7105 
                     /label="Kan/Neo"  
     misc_feature    7140..7442 
                     /label="SV40 ori"  
     misc_feature    7528..8005 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4209..4215 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4209 
                     /label="Pdzrn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTTTCGAG TTGGACCGCT TCGACGGTGA AGTGGACCCT GATCTGAAGT 
     1081 GCGCCCTGTG CCACAAGGTA CTGGAGGACC CGCTGACCAC CCCGTGCGGC CACGTATTCT 
     1141 GTGCCGGCTG TGTGCTGCCT TGGGTAGTGC AGGAGGGCAG CTGCCCCTCG CGTTGTCGCG 
     1201 GTCGCCTATC GGCCAAGGAG CTCAACCACG TCCTCCCACT CAAGCGTCTC ATCCTCAAGT 
     1261 TGGACATCAA GTGCGCGCAC GCGGCACGGG GCTGCGGCCG GGTGGTCAAG TTGCAGGACT 
     1321 TGCCCGAGCA CCTGGAGCGC TGTGACTTCG CGCCCGCGCG CTGCCGCCAC GCGGGCTGCG 
     1381 GCCAGCTGCT GCTGCGACGC GACGTGGAGG CCCACATGCG CGACGCCTGC GATGCGCGGC 
     1441 CCGTGGGTCG CTGCCAGGAG GGCTGCGGGC TGCCGCTGAC TCACGGAGAG CAGCGGGCGG 
     1501 GCGGCCACTG TTGCGCGCGG GCTTTGCGGG CGCACAACGG CGCGCTGCAG GCCCGCCTGG 
     1561 GCGCGCTGCA CAAGGCGCTC AAGAAGGAGG CGCTGCGGGC TGGCAAGCGG GAGAAGTCGC 
     1621 TGGTGGCGCA GCTGGCCGCC GCGCAGCTCG AACTGCAGAT GACCGCACTG CGCTACCAGA 
     1681 AGAAGTTCAC CGAGTACAGC GCGCGCCTCG ACTCGCTCAG CCGCTGCGTG GCCGCGCCGC 
     1741 CAGGCGGCAA GGGTGAAGAG ACCAAAAGTG TGACTCTTGT CCTGCATCGG GACTCTGGCT 
     1801 CCCTGGGATT CAATATCATT GGTGGCCGGC CCTGTGTGGA CAATCAAGAT GGATCCTCCA 
     1861 GTGAAGGAAT CTTTGTATCC AAAATAGTTG ACAGCGGGCC TGCAGCCAAG GAAGGAGGCC 
     1921 TGCAAATTCA TGACAGGATT ATTGAGGTCA ACGGCAAAGA CTTATCCCGG GCAACTCATG 
     1981 ACCAGGCTGT GGAAGCTTTC AAGACAGCCA AGGAGCCCAT CGTGGTGCAG GTGTTGAGGA 
     2041 GAACACCGCG AACCAAGATG TTCACGCCTG CCTCAGAGTC GCAGCTGGTA GACACAGGCA 
     2101 CCCAAACCGA CATCACCTTT GAGCACATCA TGGCCCTGAC AAAGATATCT TCTCCCAGCC 
     2161 CGCCCGTGCT GGGTCCCTAC CTGCTGCCTG AAGAGCACCC CTCATCCCAT GAATACTATG 
     2221 ATCCGAATGA CTACATGGGG GACATCCACC AGGACATGGA CAGGGAAGAG CTGGAGCTGG 
     2281 AGGAAGTGGG CCTCTACAGG ATGAACAGCC AGGACAAGCT GGGCCTCACC GTGTGCTACC 
     2341 GGACAGATGA CGAAGACGAC ATTGGCATTT ATATAAGTGA GATTGACCCT AACAGCATTG 
     2401 CGGCCAAGGA TGGACGGATC CGTGAAGGAG ACCGCATCAT CCAGATTAAT GGCATAGAAG 
     2461 TTCAGAACCG TGAAGAGGCC GTGGCTCTTC TAACCAGTGA AGAAAATAAG AACTTTTCAC 
     2521 TGTTGATCGC AAGGCCTGAG CTCCAGCTGG ATGAGGGCTG GATGGATGAT GACAGGAATG 
     2581 ACTTTCTGGA TGATTTGCAC ATGGATATGC TCGAGGAACA GCATCACCAG GCCATGCAGT 
     2641 TCACAGCCAG TGTGCTGCAG CAGAAGAAGC ATGAGGAAGA CGGTGGAACC ACAGACACAG 
     2701 CCACCATCTT ATCCAACCAG CACGAGAAAG ACAGTGGTGT GGGGCGAACA GATGAGAGCA 
     2761 CCCGCAATGA TGAGAGCTCG GAACAGGAGA ATAACGGCGA AGATGCCACA GCATCCTCCA 
     2821 ACCCGCTGGC GGGTCAGAGG AAACTGACCT GTAGCCAGGA CACCCTGGGC AGTGGCGACC 
     2881 TGCCTTTCAG CAACGAGTCC TTCATCTCGG CAGACTGTAC GGATGTGGAC TACCTGGGCA 
     2941 TCCCAGAGGA TGAGTGTGAG CGTTTTCGCG AGCTCTTGGA GCTCAAGTGC CAGGTACAGA 
     3001 GCACCAGCCC CTACAGCCTG TACTACCCCA ACAGCCCGCT GGATGCTGCT GGCAAGGGTG 
     3061 ATCCCGAGAG TGTGGACAAG GAGCTGGAGC TACTCAATGA GGAGTTGCGT AGCATCGAGC 
     3121 TGGAGTGCCT GAGCATCGTG CGCGCGCACA AGATGCAGCA GCTCAAGGAG CAGTACCGCG 
     3181 AGTCCTGGAT GCTGCACCAC AGTGGCTTCC GCAACTACAA CACCAGCGTG GACGTGCGCC 
     3241 GCCATGAGCT CTCGGACATC ACTGAGCTGC CGGAAAAGTC AGACAAGGAT AGTTCAAGCG 
     3301 CCTACAACAC TGGGGAGAGC TGCAGGAGTA CCCCACTCAC CCTGGAGATC TCTCCGGACA 
     3361 ACTCCCTGCG GAGAGTGGCC GAGGGCAGTA GTGAAGGGGC CACAGCTAAC ATGGAAGCTT 
     3421 ACAGGCCATC CCCCAAGAAT CTGCTTGCCA TCACTGAGGA TCCCGAAGTA AGCACCCCAA 
     3481 ACTACAACCC TGGCGCCAAA GAGCTGGACC CCAGCCAGGC TTTGGAGATC AAAGAGCGCC 
     3541 GAGTTAGCGA TGGCAGCAGG AGCCCCACCG CCAGCCCCAA GCTGGGCAGC GCCTACCTGC 
     3601 CGTCATACCA TCACTCCCCA TACAAACACG CGCACATCCC AGCTCACGCG CAACACTACC 
     3661 AGAGCTACAT GCACCTGATC CAGCAGAAGT CAGCAGTGGA GTACGCACAG AGCCAGATGA 
     3721 GCCTGGTGAG CATGTGCAAG GACTTGAACT CTTCTAACTC AGCAGAACCC AGGATGGAAT 
     3781 GGAAAGTGAA GATCCGCAGT GATGGGACGC GCTACATCAC CAAGAGGCCG GTACGTGACC 
     3841 GGCTGCTGCG GGAGCGGGCG CTGAAGATCC GCGAGGAGCG CAGTGGCCTG ACCACCGATG 
     3901 ATGACGCCAT GAGCGAGATG AAGATGGGGC GTTACTGGAG CAAGGAGGAG CGCAAGCAAC 
     3961 ATCTGGTGAA GGCCAAAGAG CAGAGGCGGA GGCGCGAGTT CATGATGCAG AGCAGGCTGG 
     4021 ATTGTCTGAA GGAGCAGCAG GCCTCCGACG ACAGGAAGGA GATGAACATC CTGGAACTGA 
     4081 GCCACAAAAA GATGATGAAG AAGAGGAACA AAAAGATCTT TGACAATTGG ATGACTATCC 
     4141 AAGAACTCTT AACCCATGGC ACAAAATCGC CAGATGGCAC TAGGGTATAC AATTCCTTCC 
     4201 TGTCGGTGAC TACTGTAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4261 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4321 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4381 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4441 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4501 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4561 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4621 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4681 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4741 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4801 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4861 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4921 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4981 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5041 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5101 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5161 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5221 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5281 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5341 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5401 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5461 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5521 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5581 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5641 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5701 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5761 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5821 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5881 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5941 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6001 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6061 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6121 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6181 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6241 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6301 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6361 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6421 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6481 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6541 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6601 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6661 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6721 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6781 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6841 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6901 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6961 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7021 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7081 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7141 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7201 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7261 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7321 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7381 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7441 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7501 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7561 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7621 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7681 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7741 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7801 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7861 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7921 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7981 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8041 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//