LOCUS       RR217446                8067 bp    DNA     circular  SYN 
DEFINITION  Itga8 (myc-DDK-tagged) - Rat integrin, alpha 8 (Itga8)
ACCESSION   NM_001173972
VERSION     NM_001173972.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4224..4315 
                     /label="Myc-DDK"  
     misc_feature    4345..4930 
                     /label="PolyA signal"  
     misc_feature    4402..4421 
                     /label="Restriction Enzyme"  
     misc_feature    5206..5878 
                     /label="ColE1"  
     misc_feature    6308..7102 
                     /label="Kan/Neo"  
     misc_feature    7137..7439 
                     /label="SV40 ori"  
     misc_feature    7525..8002 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4206..4212 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4206 
                     /label="Itga8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGCGAGA ACCCACCGGG GTCCCCCGGA GAACTGGGCA CCTCCGTTCG 
     1081 CTTGTCTCTG TTGCGTCTCG GCCGTGCTGG GGATGCTGTG GTCCCCCGTG TCTCTGGCGT 
     1141 TCAACTTGGA CGTAGACAAG CTCACGGTGT ACAGTGGCCC CGAGGGCAGC TACTTCGGCT 
     1201 ACTCGCTGGA CTTCTACATA CCTGATGCGC GCACAGCCAG TGTCTTGGTG GGGGCGCCCA 
     1261 AAGCCAACAC TAGCCAGCCG GATATCGTAG AAGGGGGAGC TGTCTACTAC TGTCCCTGGC 
     1321 CTGCAGAGAG GTCTGCACAG TGCAAGCAGA TACCCTTTGA CACCACCAAC AACAGGAAAA 
     1381 TCAGAGTTAA TGGAACCAAA GAGCCTATAG AATTTAAATC AAACCAATGG TTTGGAGCCA 
     1441 CAGTGAGAGC TCACAAAGGA AAAGTTGTGG CTTGTGCCCC TTTATATCAC TGGAGAACTC 
     1501 TGAAGCCTAA TCCAGCAAAG GACCCGGTTG GCACATGCTA TGTAGCAATT CAGAACTTCA 
     1561 GTGCTTACGC TGAGCACTCA CCCTGTCGGA ACAGCAACGT TGATCCTGAA GGCCAAGGTT 
     1621 ACTGCCAAGC CGGCTTCAGT CTAGACTTCT ATAAGAATGG AGACCTTATA GTGGGAGGAC 
     1681 CTGGAAGCTT TTACTGGCAA GGTCAGGTGA TCACGGTCAG TGTAGCAGAC ATCATTGCAA 
     1741 ATTACTCATT CAAAGACATT CTTCGAAAAC TGGCAGCAGA AAAGCAGACA GATGTGGCTC 
     1801 CAGCTTCCTA TGATGACAGC TACCTTGGAT ATTCAGTTGC TGCTGGAGAA TTCACTGGGG 
     1861 ACTCTCTGCA AGAATTGGTT GCTGGAATTC CAAGAGGAGC ACAGAACTTT GGATATGTCT 
     1921 CCATCATTAA CTCCACAGAT ATGACCTTCA TTCAGAATTT TACTGGTGAG CAGATGGCAT 
     1981 CTTATTTCGG ATATACTGTT GTGGTATCAG ATGTTAACAA TGATGGCATG GATGACATAT 
     2041 TGGTCGGGGC ACCTCTCTTT ATGGAGCGAG AATTTGAAAG TAACCCTAGA GAAGTGGGTC 
     2101 AGGTCTACCT GTACCTACAA GTGAGCGCCC TCCTCTTCCA AGACCCACAG GTCCTTACGG 
     2161 GCACAGAGAC TTTTGGGAGA TTCGGTAGTT CTGTGGCCCA CTTGGGGGAC CTGAACCAAG 
     2221 ATGGATACAA TGATATTGCC ATCGGTGTGC CCTTTGCAGG CAAGGATCAA AGAGGTAAAG 
     2281 TCCTCATTTA CAACGGAAAC GCCAGAGGCT TGCACAGCAA GCCTTCCCAA GTTCTGCAAG 
     2341 GAATATGGGG GTCACAGACC ATCCCTTCTG GATTCGGCTT TTCTCTAAGA GGAGACGCAG 
     2401 ACATAGACAA GAATGATTAC CCAGATTTAC TTGTCGGGGC ATTTGGAGAA GGGAAAGTGG 
     2461 CCGTGTACAG AGCAAGGCCA GTTGTAACAG TGGATGCCCA GCTTCTGCTG CACCCAATGA 
     2521 TTATCAACCT TGAAAATAAA ACTTGCCAGA TTCCAGAATT CCCGACTCCT GTGGCCTGCT 
     2581 TTTCTTTAAG AGTCTGTGCA TCTATAGCAG GCCAGAGCAT TTCAAATACA GTAGCCCTGA 
     2641 TGGCCGAGGT GCAGTTAGAT TTCCTGAAGC AAAAAGGAGC CATCAAACGG ACCCTCTTCC 
     2701 TCCACAACCA CCAGTCCCAC CTCATCTTCC CCTTCGTGAT GAGGCAGCAA AAATCCCTCC 
     2761 ACTGCCAGGA TTTTATGGTT TACCTTCGAG ATGAAACTGA ATTCCGAGAT AAACTGTCTC 
     2821 CAATCAACGT TAGCCTGAAC TACAGTTTGG ATGATTCCAC CTTTGAAGAT GGCCTGGAAG 
     2881 TGAAGCCGAT TTTGAACCAC TACAGGGAGA ATGTCGTTAC TGAGCAGGCT CACATCCTGG 
     2941 TGGACTGTGG AGAAGACAAC CTCTGTGTTC CTGACTTGAG GCTGTCAGCT AGACCAGATA 
     3001 AGCAGGAGAT AATTATTGGG GATGAAAATC ACCTAATGCT CATAATCAAT GCAAGGAATG 
     3061 AAGGAGAAGG AGCTTACGAA GCTGAACTCT TCGTCATGAT TCCCGAAGAA GCAGATTACG 
     3121 TTGGGATCGA GCGCAACAAC AAGGGACTGA GGCTCCTGAG TTGTGAGTAC AAGATGGAAA 
     3181 ACGTAACCAG GATGGTGGTG TGTGACCTTG GGAACCCGAT GGTGACTGGA ACGAATTTTT 
     3241 CTCTGGGCCT CCGATTTGCT GTTCCTCGTC TTGAGAAAAC AAACATGAGT ATTAACTTCG 
     3301 ATCTCCAAAT CAGAAGCTCC AACAAGGACA ATCCTGACAG CAATTTCGTG AGCGTGCAAA 
     3361 TCAACGTCAC TGCCGTGGCT CAGGTGGAAA TCAGAGGAGT GTCCCACCCT CCCCAAATTG 
     3421 TTCTGCCCAT CCACAACTGG GAGCCAGCAG AGGAGCCCCA CAAAGAGGAG GAAGTCGGTC 
     3481 CCCTGGTTGA ACATATTTAT GAGCTGCACA ATATTGGACC CAGCACCATC AGTGACAGCA 
     3541 TTCTCGAAGT GGGCTGGCCA TTCTCTGCAC GGGAGGAGTT CCTCCTCTAT GTCTTTCATC 
     3601 TTCAAACTCT GGGACCTCTA CAATGTCAAA CAAATCCTGA GATCAACCCG CAGGATATAA 
     3661 AGCCTGCTGC CTCCCCAGAG GATACCCCTG AGCTCAGCGC CTTCTTAAGA AATGCTACAA 
     3721 TTCCCCATCT TGTCAGGAAG AGGGATGTGC CAGTGGTCCA GCCCCACAGA CAGAGCCCGG 
     3781 CAAAAATACT GAACTGTACC AACATTGACT GCTTGCAAAT CTCGTGTGCA GTGGGTCGCC 
     3841 TGGGAGGAGG AGAAAGTGCA GTTCTGAAGG TCAGATCGAG ATTGTGGGCG CACACATTCC 
     3901 TCCAGAGAAA GAACGACCCT TATGCTCTTG CATCCCTGGT GTCCTTTGAA GTCAAGAAGA 
     3961 TACCTTATAA AGAGCAGCCA GCAAAACTGC CAGCCGGGAG CACAGCGATT AAGACATCGG 
     4021 TTATTTGGGC CACTCCAAAT GTCTCCTTCT CAATTCCACT GTGGGTCATA ATACTAGCGA 
     4081 TCCTTCTTGG CTTGCTGGTT CTGGCCATCT TAACTTTAGC TTTATGGAAG TGTGGATTCT 
     4141 TTGATAGAGC AAGACCTCCT CAGGATGAAA TGACAGACAG GGAACAACTG ACCAGTGACA 
     4201 AGACCCCGGA GGCTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4261 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4321 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4381 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4441 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4501 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4561 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4621 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4681 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4741 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4801 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4861 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4921 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4981 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5041 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5101 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5161 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5221 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5281 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5341 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5401 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5461 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5521 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5581 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5641 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5701 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5761 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5821 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5881 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5941 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6001 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6061 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6121 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6181 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6241 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6301 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6361 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6421 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6481 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6541 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6601 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6661 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6721 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6781 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6841 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6901 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6961 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7021 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7081 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7141 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7201 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7261 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7321 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7381 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7441 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7501 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7561 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7621 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7681 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7741 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7801 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7861 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7921 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7981 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8041 ATTTAACAAA AATTTAACGC GAATTTT
//