LOCUS       RR217443                8049 bp    DNA     circular  SYN 
DEFINITION  Kif11 (myc-DDK-tagged) - Rat kinesin family member 11 (Kif11)
ACCESSION   NM_001169112
VERSION     NM_001169112.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4206..4297 
                     /label="Myc-DDK"  
     misc_feature    4327..4912 
                     /label="PolyA signal"  
     misc_feature    4384..4403 
                     /label="Restriction Enzyme"  
     misc_feature    5188..5860 
                     /label="ColE1"  
     misc_feature    6290..7084 
                     /label="Kan/Neo"  
     misc_feature    7119..7421 
                     /label="SV40 ori"  
     misc_feature    7507..7984 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4188..4194 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4188 
                     /label="Kif11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTCCCAG CCGAGTTCTT CTTCGAAGAA GAAAGAGGAA AAGGGCAAGA 
     1081 ACATTCAGGT GGTGGTGAGA TGCAGACCAT TTAATTTGGC AGAGCGGAAA GCTAACGCCC 
     1141 ACTCTGTAGT AGAATGTGAT CACGCACGGA AGGAAGTCAG TGTACGAACT GCAGGGCTGA 
     1201 CTGACAAGAC CTCAAGGAAA ACATACACGT TTGACATGGT GTTTGGAGCA TCTACAAAAC 
     1261 AAATTGATGT TTACCGAAGT GTTGTTTGTC CAATTCTAGA TGAAGTTATT ATGGGCTATA 
     1321 ATTGTACCAT CTTCGCATAT GGCCAGACTG GCACTGGAAA AACTTTTACA ATGGAAGGTG 
     1381 AAAGGTCACC TAATGAAGTA TATACCTGGG AGGAGGATCC TCTGGCTGGT ATAATTCCAC 
     1441 GTACTCTTCA TCAGATTTTT GAGAAACTTA CTGATAATGG CACTGAGTTT TCAGTGAAAG 
     1501 TATCCCTATT GGAAATCTAT AATGAGGAGC TTTTTGATCT TCTGAGTCCG TCTTCTGATG 
     1561 TTTCTGAAAG GCTGCAGATG TTTGATGATC CCCGGAACAA GAGAGGAGTG ATAATCAAAG 
     1621 GCTTAGAGGA AATCACAGTA CACAACAAAG ATGAAGTCTA CCAAATCTTA GAGAAGGGGG 
     1681 CAGCCAAAAG GACAACTGCA GCAACCTTGA TGAATGCTTA CTCCAGTCGT TCACACTCAG 
     1741 TTTTTTCTGT CACGATACAC ATGAAAGAAA CGACAATTGA TGGAGAAGAG CTTGTTAAAA 
     1801 TTGGCAAGTT GAATTTGGTT GATCTTGCAG GAAGTGAAAA TATTGGGCGT TCTGGAGCTG 
     1861 TTGACAAGAG GGCCCGGGAA GCTGGAAATA TCAACCAGTC CCTGCTGACT CTGGGGAGAG 
     1921 TTATCACTGC TCTTGTGGAA AGAACACCTC ATATTCCTTA TCGAGAATCT AAACTAACAA 
     1981 GAATCCTGCA AGATTCTCTT GGGGGGCGTA CAAGGACATC TATAATTGCA ACCATTTCCC 
     2041 CTGCGTCTCT CAATCTTGAG GAAACTCTGA GTACATTGGA ATATGCTCAC AGAGCAAAGA 
     2101 ACATAATGAA TAAGCCTGAA GTTAATCAGA AACTCACCAA AAAGGCACTT ATTAAGGAGT 
     2161 ATACAGAAGA GATAGAGCGT TTGAAGCGAG ATCTTGCAGC AGCTCGTGAG AAAAATGGAG 
     2221 TGTACATCTC TGAAGAAAGT TTCAGAGCCA TGAATGGAAA AGTAACTGTT CAGGAGGAAC 
     2281 AAATTGCTGA GTTGGCTGAA AAAATTGGTG TCCTTGAGGA GGAGCTCAGT AAGGCTGCAG 
     2341 AGTTATTTAC GGATAGTGAA AAGGAACTTA ATCAGTGTAA ATCTGACCTG CAAACCAAGA 
     2401 CACAGGAACT TGAAACCACT CAGAAACATT TGCAAGAAAC AAAATTACAA CTTGTTAAAG 
     2461 AGGAGTATGT CTCTTCAGCT TTGGAAAGAA CTGAGAAGAA GCTTCATGAA ACGGCCAGCA 
     2521 AGTTGCTTAA CACAGTTAAA GAAACCACCA GGGACGTATC TGGTCTGCAT TCTAAACTGG 
     2581 ACCGGAAGAG AGCAATCGAT GCGCACAACG CTGAGGCTCA GGAGAGCTTT GGCAGGAGCC 
     2641 TCAGCAGTCT GTTTAATAAT ATGGAAGAGT TGATCAGGGA CGGCAGTGAG AAACAGAAGG 
     2701 CCATGCTGGA CGTTCACAAA GCACTGTTCG GTAACCTGAT GTCTTCTAGT GTCTCTGCAT 
     2761 TAGACACTAT TTCCACGACA GCACTTGAAT CTCTCATGTC TATTCCAGAA AACGTGTTAG 
     2821 CTCGTGTTTC TCAGATTTCT GATATGATAT TGGAAGAGCA GTCGTTAGCA GCCCAGAGTA 
     2881 AAACTGTGCT GCAAGGATCG ATTGATGAAC TTGTGACCAA CCTTTTCACG TCCCTGAAGA 
     2941 CCATCATAGC TCCTGGTGTG GTGTCCATCT TAAACATAAA TAGGCAACTA CAGCATATTT 
     3001 TCAGGGCTTC GGTGACAATG GCTGAAAAGG TAGAAGATCA AAAAAGAGAA ATAGACAGTT 
     3061 TTCTCAGCAT ATTGTGTAAC AATTTACATG AACTCCGAGA AAACACTGTT TCTTCCTTGG 
     3121 TTGAATCACA AAAGCTTTGT GGAAACCTAA CTGAAGACCT GAAGACAATA AAGGAAACCC 
     3181 ATTCACAGGA ACTTTGCCAG TTAAGCAGTA GTTGGGCAGA GAGATTCTGT GCTTTGGAGA 
     3241 AGAAGTATGC AAACATCCAG AAACCACTGA ACAGTATTCA AGAAAACACA GAGCGGAAGT 
     3301 CTACTGATAT AATCAATAAA ACAACAGTTC ACAGTAAGAA AATTCTTGCA GACTCTGATG 
     3361 GATTATTACA AGAACTCAGA CACTTTAACG AAGAAGGTAC ACAGTTGGTT GAAGACTCTG 
     3421 TAGGACACTG TGGTTCACTC AGCAGCAACC TGGAGACTGT ATCCCAAGAG ATCACACAGA 
     3481 GGTGTGGGAC CCTGAACACA AGCACAGTTC ATTTTTCTGA TCAGTGGGCA TCCTGCTTAA 
     3541 GCAAGAGGAA GGAAGAGCTT CAGAACTTAA TGGAGTTTGT AAATGGATGT TGTGAAGCTT 
     3601 CAAGTTCAGA GATCACTGAA AAAATAAGAG GACACAGAGC AGCTATTGAG AGCCAGCACA 
     3661 GCTCCTTTGT TGTTCAGATA ACTTCTGATG AAGAAAAATT TAAAGCTGGA AGCCTAGAGC 
     3721 TTGATAAAAC TATAAAAACT GGTTTAACAA AGCTTAATTG CTTTCTGCAA CAGGATCTGA 
     3781 AACTAGATAT CCCAACAGGT ATGACACCAG AGAGGAAAAA TTATTTATAT CCATCAACAC 
     3841 TTGTGAGAAC TGAACCACGG GAGCAACTCC TTGATCAGCT GCAAAAGAAA CAGCCCAAGC 
     3901 CACTGATGAT GCTAAATTGC TCAGAAAGCA GCAAAGAGAC CAGTCAGGAC ATGGAGGAGG 
     3961 ACAGGGAGGT TCTGGAGCAG AGTACTGAAG AACTCGTAAG TCAAGAGCCG TGTGTACATA 
     4021 CCAGTGCGGA TTGCTCTTCC AGTGGAGGTG TTCCATTTTT TCAGCATAAA AAGCCACATG 
     4081 GAAAAGACAA AGAAAACAGA GGCCTTAACC CCGTGGAGAA GGGTAAAGTG GAGGAGGCCT 
     4141 CGGAGCACTC CATCTCCAAG AGCAGACTGC CGCTGCGCGC CTCCATCAAC CTCTGCACGC 
     4201 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4261 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4321 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4381 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4441 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4501 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4561 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4621 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4681 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4741 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4801 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4861 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4921 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4981 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5041 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5101 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5161 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5221 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5281 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5341 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5401 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5461 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5521 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5581 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5641 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5701 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5761 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5821 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5881 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5941 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6001 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6061 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6121 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6181 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6241 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6301 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6361 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6421 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6481 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6541 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6601 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6661 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6721 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6781 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6841 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6901 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6961 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7021 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7081 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7141 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7201 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7261 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7321 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7381 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7441 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7501 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7561 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7621 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7681 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7741 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7801 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7861 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7921 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7981 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8041 GCGAATTTT
//