LOCUS       RR217427                7992 bp    DNA     circular  SYN 
DEFINITION  Atp4a (myc-DDK-tagged) - Rat ATPase, H+/K+ exchanging, alpha polypeptide (Atp4a)
ACCESSION   NM_012509
VERSION     NM_012509.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4147..4238 
                     /label="Myc-DDK"  
     misc_feature    4268..4853 
                     /label="PolyA signal"  
     misc_feature    4325..4344 
                     /label="Restriction Enzyme"  
     misc_feature    5129..5801 
                     /label="ColE1"  
     misc_feature    6231..7025 
                     /label="Kan/Neo"  
     misc_feature    7060..7362 
                     /label="SV40 ori"  
     misc_feature    7448..7925 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4122..4129 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4122 
                     /label="Atp4a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAGGCA GAGAATTATG AATTGTACTC AGTGGAACTG GGGACTGGCC 
     1081 CTGGTGGGAA CATGGCTGCC AAGATGAGCA AGAAGAAGGC GGGAGGCGGG GGAGGCAAGA 
     1141 AGAAGGAGAA GCTAGAGAAC ATGAAGAAGG AGATGGAGAT GAACGACCAC CAGTTGTCAG 
     1201 TGTCTGAGCT GGAGCAGAAG TACCAGACCA GTGCCACCAA GGGCCTGAAG GCGAGTCTGG 
     1261 CAGCTGAGCT GCTGCTGAGG GATGGGCCCA ATGCACTCCG GCCACCTCGG GGCACCCCTG 
     1321 AGTACGTGAA GTTCGCCCGG CAGCTGGCAG GCGGTCTGCA GTGCCTCATG TGGGTGGCTG 
     1381 CAGCCATCTG CCTCATTGCC TTTGCGATTC AGGCCAGCGA GGGAGACCTG ACCACTGATG 
     1441 ACAATTTGTA CCTGGCGTTG GCACTCATTG CTGTGGTTGT GGTCACTGGC TGTTTTGGCT 
     1501 ACTACCAGGA GTTCAAGAGC ACAAATATCA TCGCCAGCTT CAAGAATCTT GTACCCCAGC 
     1561 AAGCCACAGT GATCCGAGAC GGGGATAAGT TCCAGATCAA CGCGGATCAG CTTGTGGTGG 
     1621 GCGACCTGGT AGAGATGAAA GGCGGGGACC GCGTCCCAGC AGACATCCGA ATTCTGTCAG 
     1681 CCCAGGGCTG CAAGGTGGAC AACTCCTCGC TTACTGGAGA GTCTGAACCG CAGACCCGCT 
     1741 CACCTGAGTG TACACACGAG AGTCCCCTTG AGACCCGCAA CATCGCCTTC TTCTCCACCA 
     1801 TGTGTCTGGA GGGCACGGCG CAGGGCTTGG TGGTGAGCAC CGGTGATCGC ACCATCATTG 
     1861 GACGCATCGC CTCTCTGGCT TCGGGTGTGG AAAACGAGAA GACTCCTATC GCGATCGAGA 
     1921 TCGAACATTT TGTGGACATC ATTGCTGGCC TGGCCATCCT CTTCGGTGCC ACATTCTTTG 
     1981 TGGTGGCCAT GTGTATCGGC TATACCTTCC TTCGGGCCAT GGTCTTCTTC ATGGCCATTG 
     2041 TGGTAGCCTA TGTGCCTGAG GGGCTGCTGG CTACTGTCAC GGTCTGCCTG TCACTGACAG 
     2101 CAAAGAGGCT GGCCAGTAAA AACTGTGTGG TCAAAAATCT GGAAGCAGTG GAGACCCTGG 
     2161 GTTCCACGTC AGTCATCTGC TCAGACAAGA CAGGAACTCT TACTCAGAAC CGCATGACGG 
     2221 TGTCTCATCT ATGGTTTGAC AACCATATCC ACACGGCGGA CACCACAGAA GACCAGTCAG 
     2281 GGCAAACGTT CGACCAATCG TCGGAGACCT GGCGGGCGCT GTGCCGCGTG CTCACCCTGT 
     2341 GCAACCGCGC TGCCTTCAAG TCTGGCCAGG ACGCCGTGCC AGTGCCCAAG CGCATCGTGA 
     2401 TCGGAGACGC ATCTGAGACT GCGCTGCTCA AGTTCTCGGA GTTGACGTTG GGCAACGCCA 
     2461 TGGGTTATCG GGACCGCTTC CCCAAAGTTT GCGAGATCCC CTTCAACTCC ACCAACAAGT 
     2521 TTCAGCTGTC CATTCACACT CTGGAGGATC CGCGCGACCC CCGGCACTTG CTGGTGATGA 
     2581 AGGGCGCCCC AGAGCGCGTG CTGGAGCGTT GCAGCTCCAT CCTCATCAAG GGCCAGGAGC 
     2641 TGCCCCTGGA CGAGCAATGG CGTGAGGCCT TCCAGACAGC CTACCTTAGC CTGGGAGGCC 
     2701 TAGGCGAACG CGTTCTTGGT TTCTGCCAGC TCTACCTGAA TGAGAAGGAC TACCCGCCTG 
     2761 GCTACACCTT TGATGTGGAG GCCATGAACT TTCCAAGTAG TGGCCTCTGC TTTGCGGGAC 
     2821 TTGTATCCAT GATTGACCCT CCCCGGGCCA CCGTTCCAGA TGCTGTGCTC AAATGCCGTA 
     2881 CGGCAGGCAT CCGGGTGATC ATGGTGACTG GTGACCATCC CATCACAGCC AAGGCCATTG 
     2941 CAGCCAGTGT GGGGATCATC TCGGAAGGCA GCGAGACAGT GGAAGACATC GCCGCCCGCC 
     3001 TCCGAATGCC TGTAGACCAG GTTAATAAGA AGGATGCCCG GGCCTGTGTG ATCAATGGCA 
     3061 TGCAGTTGAA GGACATGGAC CCATCTGAGC TGGTGGAGGC GCTGCGCACC CACCCTGAGA 
     3121 TGGTGTTTGC TCGAACCAGT CCTCAGCAGA AGCTGGTGAT TGTGGAGAGC TGTCAGCGAC 
     3181 TGGGTGCCAT TGTGGCTGTA ACAGGGGATG GTGTGAATGA CTCCCCAGCC CTGAAGAAGG 
     3241 CTGACATTGG TGTAGCCATG GGCATTGCTG GCTCCGATGC TGCTAAAAAT GCTGCTGACA 
     3301 TGATCTTGCT GGATGACAAC TTCGCTTCCA TTGTGACGGG CGTGGAGCAG GGCCGACTGA 
     3361 TCTTTGACAA CCTGAAGAAA TCCATCGCCT ATACACTGAC CAAGAACATT CCAGAACTGA 
     3421 CACCCTACCT TATCTATATC ACTGTCAGTG TGCCCCTACC CCTTGGGTGT ATCACCATTC 
     3481 TCTTCATAGA ACTCTGCACA GATATCTTTC CATCTGTGTC CCTGGCATAT GAAAAGGCCG 
     3541 AGAGTGACAT CATGCACCTG CGCCCACGGA ACCCCAGGCG GGACCGGTTG GTCAATGAAC 
     3601 CCCTGGCTGC TTATTCCTAT TTTCAGATTG GTGCCATTCA GTCATTTGCC GGCTTTGCTG 
     3661 ACTACTTCAC GGCCATGGCC CAGGAGGGCT GGTTCCCTCT GCTGTGTGTG GGGCTGCGAC 
     3721 CACAATGGGA GGACCACCAT CTACAAGATC TTCAAGACAG CTACGGCCAG GAATGGACAT 
     3781 TTGGTCAGCG TCTGTACCAG CAGTACACCT GTTACACCGT GTTCTTCATC AGCATCGAGA 
     3841 TGTGCCAGAT CGCTGATGTC CTCATCCGCA AGACACGCCG CCTCTCCGCT TTCCAGCAGG 
     3901 GATTCTTCAG GAACAGGATC CTGGTGATCG CCATCGTGTT TCAGGTCTGC ATTGGCTGCT 
     3961 TCCTGTGCTA CTGCCCAGGG ATGCCCAACA TCTTCAACTT CATGCCCATC CGGTTCCAGT 
     4021 GGTGGCTGGT CCCCATGCCC TTTGGCCTTC TCATCTTTGT CTATGATGAG ATCCGGAAAC 
     4081 TTGGAGTTCG CTGTTGCCCA GGGAGCTGGT GGGACCAGGA ACTCTACTAT AGCGGACCGA 
     4141 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4201 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4261 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4321 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4381 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4441 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4501 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4561 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4621 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4681 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4741 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4801 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4861 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4921 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4981 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5041 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5101 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5161 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5221 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5281 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5341 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5401 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5461 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5521 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5581 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5641 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5701 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5761 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5821 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5881 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5941 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6001 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6061 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6121 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6181 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6241 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6301 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6361 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6421 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6481 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6541 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6601 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6661 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6721 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6781 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6841 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6901 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6961 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7021 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7081 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7141 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7201 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7261 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7321 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7381 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7441 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7501 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7561 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7621 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7681 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7741 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7801 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7861 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7921 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7981 AACGCGAATT TT
//