LOCUS       RR217404                7881 bp    DNA     circular  SYN 
DEFINITION  Flt3 (myc-DDK-tagged) - Rat fms-related tyrosine kinase 3 (Flt3)
ACCESSION   NM_001100822
VERSION     NM_001100822.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4038..4129 
                     /label="Myc-DDK"  
     misc_feature    4159..4744 
                     /label="PolyA signal"  
     misc_feature    4216..4235 
                     /label="Restriction Enzyme"  
     misc_feature    5020..5692 
                     /label="ColE1"  
     misc_feature    6122..6916 
                     /label="Kan/Neo"  
     misc_feature    6951..7253 
                     /label="SV40 ori"  
     misc_feature    7339..7816 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4020..4026 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4020 
                     /label="Flt3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGCGTTG GCGCGGCGCA GCGACCGGCT GCCGCTGCTT GTTGTTTTGT 
     1081 CAGTAATGAT TCTCGAGACC GTTACAAACC AAGATCTGCC TGTGATCAAG TGTGTTTTAA 
     1141 TCATTCATAA GAACAATGGC TCGGCGGCGG GAAAGTCATC GTCGCACGTC ATGGTTCCAG 
     1201 AATCCCCAGA GGACCTCCGG TGTGCCCCGA AGCATCGGAG TGAAGGGGTG GTCTATGAAG 
     1261 CTGTCACTGT GGAGGTGGCC CAGTCTGCGT CCTTCACATT GCAAGTACAG CTCACCACCC 
     1321 CGGGGGATGT TTCTTGCCTC TGGGTCTTTA AGCACAGCTC GCTGGGCTGC CAGCCACACT 
     1381 TTGATTTACA GAACAGAAGA ATTGTTTCCA TGACCATCTT GAACGTTACA GAGACCCAGG 
     1441 CAGGAGAATA CCTACTCCAT ATTCAGAGTG AAGCCACCAA CCACACGGTG CTGTTCACAG 
     1501 TGAATGTAAG AGATACACAG CTGTACGTGC TAAGAAGACC TTCCTTCAGA AAGATGGAGA 
     1561 ACCAGGACGC CCTGCTCTGC GTCTCTGAGG GTGTCCCAGA GCCCACCGTG GAGTGGGTGG 
     1621 TCTGCAGTTC CCACAGGGAC AGCTGTAGAG AAGAAGGCTC TGCCGTTGTC AAAAAGGAGG 
     1681 AAAAGGTACT TCACGAGCTA TTTGAAACAG ACATCAGATG CTGTGCGAGA AATGCACTGG 
     1741 GCCGAGAATG CACCAAGCTG TTCACCATAG ATCTAAACCA GGCTCCTCAG AGCACACTGC 
     1801 CCCAGTTATT TCTGAAAGTG GGGGAGCCCT TGTGGATAAG GTGCAAGGCC ATCCATGTGA 
     1861 ACCATGGATT CGGGCTCACC TGGGAGCTGG GAAACAAAGT CCTGGAGGAG GGCAGCTACT 
     1921 TTGAGATGAG TTCCTACTCC ACCAACAGAT CCATGATCCG GATTCTCTTG GCTTTTGTGT 
     1981 CCTCTGTGGG AAGGAACAAC ACCGGATATT ACACCTGTTC TTCCTCAAAG CATCCCAGCC 
     2041 AGACAGCGTT GGTGACCATC CTAGAAAAAG GTTTCATAAA TGCTACCAGC TCACAAGAAG 
     2101 AGTATGAAAT TGACCCGTAT GAAAAGTTCT GCTTCTCGGT CAGGTTTAAA GCGTACCCAC 
     2161 AAATCCGATG CAAGTGGACC TTCTCTCAAA CATCGTTTCC TTGTGAACAA AGTGGCCTGG 
     2221 AGGATGGGTA CAGCATTTCT AAATTTTGCG ATCATAAGAA CAACCCAGGA GAATACATAT 
     2281 TCCATGGAGA AAATGATGAC GCCCAGTTCA CCAAAATGTT CATACTGAAT ATAAGAAGGA 
     2341 AACCTCAAGT GCTAGCAAAT GCCTCAGCCA GCCAGGCATC CTGTTTCTCT GATGGCTACC 
     2401 CGTTACCCAA CTGGACCTGG AAGAAGTGTT CGGACAAGTC ACCCAACTGC ACGGAGGAAA 
     2461 TCCCAGAAGG AGTTTGGAAT AAAAAGGCTA ACAGAAAAGT GTTTGGACAG TGGGTGTCGA 
     2521 GCAGTACCCT GAATATGAGT GAGGCCGGGA GAGGGTTTCT GGTCAAATGC TGTGCATACA 
     2581 ATTCTATGGG CACGTCTTGC GAAACCATCC TTTTAAACTC ACCAGGCCCC TTCCTTTTCA 
     2641 TCCAAGACAA CATCTCCTTC TATGCAACCA TCGGGCTTTG CCCGCCTTTC ATTGTCGTCC 
     2701 TCACTGTGCT GATCTGTCAC AAATACAAAA AGCAATTTAG GTACGAAAGC CAGCTGCAGA 
     2761 TGATCCAAGT GACCGGCCCC CTGGATAACG AGTACTTCTA CATTGACTTC AGGGACTACG 
     2821 AATATGACCT CAAGTGGGAG TTTCCAAGGG AAAACTTAGA GTTTGGGAAG GTCCTGGGGT 
     2881 CCGGCGCTTT TGGGAGAGTG ATGAATGCCA CGGCCTACGG GATTAGTAAA ACAGGAGTCT 
     2941 CAATTCAGGT GGCGGTGAAG ATGCTAAAAG AGAAAGCTGA CAGATGTGAA AGAGAGGCGC 
     3001 TCATGGCTGA GCTCAAAATG ATGACCCACC TGGGGCACCA TGACAACATC GTGAACCTGC 
     3061 TGGGGGCATG CACACTGTCA GGGCCAGTGT ACTTGATTTT TGAATATTGT TGCCATGGTG 
     3121 ACCTGCTCAA CTACCTAAGA AGCAAAAGAG AAAAGTTTCA CAGGACGTGG ACAGAGATTT 
     3181 TTAAGGAACA TAATTTCAGT TTTTACCCCA CCTTCCAGTC ACATTCAAAC TCCAGTATGC 
     3241 CGGGTTCACG AGAAGTTCAG ATATACCCGC CCGTGGATCA GGTCTCAGGG TTCAATGGGA 
     3301 ATTCAATTCA TTCTGAAGGT AATATTGAGT ATGAAAACCA GAAGAGGCTG GAAGAAGAGG 
     3361 AGGAAGAAGA TTTGAACGTG CTGACGTTTG AAGACCTCCT TTGTTTTGCG TATCAAGTGG 
     3421 CCAAGGGCAT GGAGTTCCTG GAGTTCAAGT CGTGTGTTCA CAGAGACCTG GCAGCCAGGA 
     3481 ACGTACTGGT CACCCACGGG AAGGTGGTGA AGATCTGTGA CTTTGGACTG GCCCGAGACA 
     3541 TCCTGAGTGA CTCCAGCTAC GTCGTCAGGG GCAACGCACG GCTGCCAGTG AAGTGGATGG 
     3601 CACCTGAGAG CTTGTTTGAA GGGATCTATA CAATCAAGAG TGACGTCTGG TCCTACGGCA 
     3661 TCCTTCTCTG GGAGATATTT TCATTGGGTG TGAACCCTTA CCCCGGTATT CCTGTCGATG 
     3721 CTAACTTCTA TAAACTGATT CAGAGTGGAT TTAAAATGGA GCAGCCGTTC TATGCTACGG 
     3781 AAGAGATATA CTTTGTAATG CAATCCTGCT GGGCTTTTGA CTCAAGGAAG CGGCCATCCT 
     3841 TCCCCAATCT GACGTCATTT TTAGGCTGTC AGCTCGCAGA GGCAGAAGCA GCGATGTATC 
     3901 AGAACATGGG CGGCGATGTC CCACTGGAGC ACCCAGCCAT CTACCAAAAC AGGCGGCCTG 
     3961 CCAGCAGAGA GGCAGGCTCA GAGCCGCCAT CACCACAGGT TCAGGAGAAG ACTCACAGAG 
     4021 AAAGAAGTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4081 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4141 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4201 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4261 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4321 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4381 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4441 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4501 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4561 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4621 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4681 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4741 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4801 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4861 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4921 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4981 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5041 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5101 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5161 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5221 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5281 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5341 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5401 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5461 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5521 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5581 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5641 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5701 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5761 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5821 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5881 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5941 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6001 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6061 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6121 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6181 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6241 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6301 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6361 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6421 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6481 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6541 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6601 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6661 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6721 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6781 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6841 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6901 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6961 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7021 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7081 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7141 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7201 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7261 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7321 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7381 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7441 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7501 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7561 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7621 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7681 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7741 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7801 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7861 CAAAAATTTA ACGCGAATTT T
//