LOCUS       RR217396                7863 bp    DNA     circular  SYN 
DEFINITION  Scube3 (myc-DDK-tagged) - Rat signal peptide, CUB domain, EGF-like 3 (Scube3)
ACCESSION   NM_001271362
VERSION     NM_001271362.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4020..4111 
                     /label="Myc-DDK"  
     misc_feature    4141..4726 
                     /label="PolyA signal"  
     misc_feature    4198..4217 
                     /label="Restriction Enzyme"  
     misc_feature    5002..5674 
                     /label="ColE1"  
     misc_feature    6104..6898 
                     /label="Kan/Neo"  
     misc_feature    6933..7235 
                     /label="SV40 ori"  
     misc_feature    7321..7798 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4002..4008 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4002 
                     /label="Scube3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTCGGGG CGCGTCCCCG GGCTCTGCCT GCTCGTCCTG CTGGTCCACG 
     1081 CTCGCGCCGC CCAGCATGGC AAAGCTGCGC AAGATGTAGA TGAGTGTGTG GAGGGCACGG 
     1141 ATCACTGTCA CATTGATGCC ATCTGCCAGA ACACCCCACG GTCATACAAG TGCATCTGCA 
     1201 AGTCCGGCTA CACGGGGGAT GGCAAGCATT GCAAAGACGT GGATGAGTGT GAACGGGAGG 
     1261 ACAACGCTGG CTGTGTGCAC GACTGTGTCA ACATCCCTGG TAATTACCGG TGTACCTGCT 
     1321 ACGACGGGTT CCACCTGGCA CACGACGGAC ACAACTGTCT GGACGTGGAT GAGTGTGCAG 
     1381 AGGGCAATGG GGGCTGCCAG CAGAGCTGTG TCAACATGAT GGGCAGCTAT GAATGCCACT 
     1441 GCCGAGATGG CTTCTTCCTC AGTGACAACC AGCACACCTG TATCCAGCGG CCAGAAGAAG 
     1501 GAATGAACTG CATGAACAAG AACCACGGCT GTGCCCACAT TTGCCGGGAG ACTCCGAAGG 
     1561 GGGGCATTGC CTGTGAATGC CGCCCTGGCT TCGAGCTCAC CAAGAACCAA CGGGACTGTA 
     1621 AATTGACCTG CAACTACGGT AACGGTGGCT GCCAGCACAC ATGCGATGAC ACAGAGCAGG 
     1681 GTCCTCGGTG CGGCTGCCAC GTCAAGTTTG TGCTCCATAC GGACGGAAAG ACATGCATTG 
     1741 AGACCTGTGC CGTCAACAAC GGAGGCTGTG ACAGTAAGTG TCACGATGCA GCGACCGGCG 
     1801 TCCACTGCAG CTGCCCTGTG GGTTTCATGC TGCAGCCAGA CAGAAAGACC TGCAAAGACA 
     1861 TAGACGAGTG CCGCTTACAC AACGGGGGCT GTGACCACAT CTGCCGCAAC ACAGTGGGCA 
     1921 GCTTTGAATG CAGCTGTAAG AAGGGCTACA AGCTTCTCAT CAACGAGAGG AGCTGCCAGG 
     1981 ATATAGATGA GTGTTCCTTT GATCGAACCT GTGACCACAT GTGTGTCAAC ACTCCGGGAA 
     2041 GCTTCCAGTG TCTCTGTCAT CGAGGCTACC TTCTGTATGG CATCTCTCAT TGTGGGGATG 
     2101 TGGATGAATG CAGCATCAAT AAGGGGGGCT GCCGCTTTGG CTGCATCAAC ACCCCAGGCA 
     2161 GCTATCAGTG TACCTGCCCG GCAGGCCAGG GCCGCCTCCA CTGGAACGGA AAGGATTGCA 
     2221 CAGAGCCAGT GAAGTGCCAG AGCAGTTCTG GAGCCTCAAA AGCCATGCTC AGCTGCAGTC 
     2281 GCTCGGGCAA GAAGGATACC TGTGCCCTGA CCTGCCCCTC CCGCGCCCGG TTCTTACCAG 
     2341 AGTCAGAGAA CGGATTCACA GTGAGCTGCG GTACCCCGAG CCCTAAAGCT GCTGCAGGTC 
     2401 GAGCCATTCA CCCGGGCAAC AGCACCATCT CCAACTCCTG CCATGAGGCT GCAGTGCTGC 
     2461 CAGTCAAGCA GAGGGCCTCC TTCAAGATCA AGGACGCCAA ATGTCGTCTG CATCTGCGAA 
     2521 ACAAAGGCAA AGCGGAGGAG GCCAGCGGCA GAACCCCGGG GCCAGGTGGT GCCTCCTGCT 
     2581 CTGACTGCCA GGTCACCTTT ATCCATCTTA AGTGTGACTC TTCTCGGAAG GGCAAGGGCA 
     2641 GACGGGCCCG GACCCCTCCA GGCAAGGAGG TTACCCGACT CACCCTGGAA TTAGAAGCTG 
     2701 AGGTCAGAGC AGAAGAAACG ACAGCTGGCT GTGGGCTGAC TTGCCTCCGA CAGCGGATGG 
     2761 AACGAAGGCT GAAAGGGTCC CTGAAGATGC TCAGAAAGTC CATCAACCAG GACCGGTTCC 
     2821 TGCTGCGTCT GGCCGGCCTT GACTATGAGC TAGCCCACAA GCCAGGCCTG GCAGCTGGGG 
     2881 ACCGAGCAGA GCTAGTGGAG GTCTGCCGAC CCGGGCAGCA CCGAGCAGGC ACCAAGTGTG 
     2941 TCAGCTGCCC GCAGGGAACG TATTACCACG GCCAGACGGA GCAGTGTGTG CCATGCCCAG 
     3001 CGGGCACCTT CCAGGAGAGG GAAGGGCAGC TCTCCTGCGA CCTTTGCCCT GGGAGTGATG 
     3061 CCCACGGGCC TCTTGGAGCC ACCAACGTCA CCACGTGTGC AGGTCAGTGC CCACCTGGTC 
     3121 AACACTCAGG GGATGGATTT AAGCCCTGCC AGCCCTGCCC ACGTGGCACC TATCAGCCCG 
     3181 AAGCAGGACG AACCCTGTGC TTCCCCTGTG GCGGAGGCCT CACTACCAAG CACGAGGGGG 
     3241 CAGTCTCCTT CCAGGACTGT GACACCAAAG TCCAGTGCTC CCCGGGCCAC TACTACAACA 
     3301 CTAGCATCCA TCGCTGTATC CGCTGTGCTG TGGGCTCCTA CCAGCCAGAC TTCCGCCAGA 
     3361 ACTTCTGCAC CCGCTGCCCA GGAAATACAA GCACGGACTT CGATGGCTCT ACCAGTGTGG 
     3421 CCCAGTGCAA GAATAGGCAG TGTGGCGGGG AGCTGGGTGA GTTCACTGGC TACATCGAGT 
     3481 CTCCCAACTA CCCGGGTAAC TACCCAGCTG GTGTGGAGTG CATCTGGAAC ATCAACCCCC 
     3541 CACCCAAGCG CAAGATCCTC ATTGTGGTCC CTGAAATCTT CCTGCCGTCC GAGGACGAGT 
     3601 GTGGGGACGT CCTGGTCATG AGAAAGAACT CCTCCCCATC CTCCATCACC ACGTATGAGA 
     3661 CCTGCCAGAC ATACGAGCGG CCCATCGCCT TCACCGCCCG GTCCAGGAAA CTCTGGATCA 
     3721 ACTTCAAGAC CAGCGAGGCC AACAGCGCGC GTGGTTTTCA GATTCCCTAT GTAACCTACG 
     3781 ATGAGGACTA TGAGCAGCTG GTGGAAGATA TAGTGCGGGA TGGCCGGCTG TACGCCTCTG 
     3841 AGAACCACCA GGAGATCCTA AAGGATAAGA AGCTTATCAA GGCCTTCTTT GAGGTCCTGG 
     3901 CCCACCCCCA AAACTACTTC AAGTACACAG AGAAACACAA GGAGATGCTG CCAAAGTCCT 
     3961 TCATCAAGTT ACTGCGCTCC AAGGTCTCCA GCTTCCTGAG GCCTTACAAA ACGCGTACGC 
     4021 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4081 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4141 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4201 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4261 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4321 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4381 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4441 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4501 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4561 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4621 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4681 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4741 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4801 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4861 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4921 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4981 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5041 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5101 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5161 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5221 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5281 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5341 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5401 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5461 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5521 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5581 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5641 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5701 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5761 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5821 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5881 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5941 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6001 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6061 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6121 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6181 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6241 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6301 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6361 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6421 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6481 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6541 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6601 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6661 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6721 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6781 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6841 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6901 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6961 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7021 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7081 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7141 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7201 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7261 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7321 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7381 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7441 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7501 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7561 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7621 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7681 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7741 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7801 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7861 TTT
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