LOCUS       RR217391                7851 bp    DNA     circular  SYN 
DEFINITION  Bmp1 (myc-DDK-tagged) - Rat bone morphogenetic protein 1 (Bmp1)
ACCESSION   NM_031323
VERSION     NM_031323.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4008..4099 
                     /label="Myc-DDK"  
     misc_feature    4129..4714 
                     /label="PolyA signal"  
     misc_feature    4186..4205 
                     /label="Restriction Enzyme"  
     misc_feature    4990..5662 
                     /label="ColE1"  
     misc_feature    6092..6886 
                     /label="Kan/Neo"  
     misc_feature    6921..7223 
                     /label="SV40 ori"  
     misc_feature    7309..7786 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3990..3996 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3990 
                     /label="Bmp1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGGCGTG GCCCGCCCGC CGCTGCCGCT GCTGTCGCTG CCACTGCTGC 
     1081 TACTGCTGCT GCTCCCGCGC GCCGGCCGGC CGCTGGACTT GGCCGACTAC ACCTACGACC 
     1141 TGGGCGAGGA GGACGCCCCG GAGCTCCTCA ACTACAAAGA CCCCTGCAAG GCGGCTGCCT 
     1201 TTCTTGGGGA CATTGCCCTG GATGAGGAGG ACTTGAGGGC CTTCCGGGTG CAGCAGGCTG 
     1261 CAGTTCTCAG ACAACAAACA GCCCAGAGGT CGTCCATCAA AGCTGCAGGG AACTCTTCTG 
     1321 CCCTGGGTAG ACAGAGCACG AGTGGGCAGC CGCAGAGGGG AAGCAGAGGC AGATGGAGAA 
     1381 GCAGGCCTCG GAGCAGGCGG GCAGCGACAT CCAGACCAGA GCGAGTGTGG CCCGATGGGG 
     1441 TCATCCCATT TGTCATCGGA GGGAATTTCA CTGGCAGCCA GAGGGCAGTC TTCCGGCAGG 
     1501 CCATGAGACA CTGGGAGAAG CATACCTGTG TCACCTTCTT GGAGCGCACA GATGAGGACA 
     1561 GCTATATTGT ATTCACCTAC CGACCCTGCG GGTGCTGCTC CTACGTGGGT CGCCGAGGTG 
     1621 GGGGCCCCCA GGCCATCTCC ATCGGCAAGA ACTGTGACAA GTTTGGCATC GTGGTCCATG 
     1681 AGCTGGGCCA TGTCATTGGC TTCTGGCACG AGCACACGCG GCCCGACCGG GACCGCCATG 
     1741 TCTCTATTGT ACGGGAGAAC ATACAGCCAG GGCAGGAGTA TAACTTCTTG AAGATGGAAG 
     1801 TTCAGGAAGT CGAGTCCTTG GGAGAGACCT ATGACTTTGA CAGTATCATG CACTATGCCC 
     1861 GGAACACGTT CTCCAGGGGC ATCTTCCTGG ACACCATTGT TCCCAAGTAT GAGGTGAATG 
     1921 GGGTGAAGCC TTCCATTGGC CAAAGGACCC GACTCAGCAA GGGGGACATC GCTCAGGCCC 
     1981 GGAAGCTCTA CAAATGCCCA GCCTGTGGTG AGACCCTCCA AGACAGCACT GGCAACTTCT 
     2041 CCTCCCCTGA GTATCCCAAT GGCTACTCTG CCCACATGCA CTGTGTATGG CGTATCTCTG 
     2101 TCACACCGGG GGAGAAGATT ATTCTGAACT TCACATCTAT GGACCTGTAC CGTAGCCGCC 
     2161 TGTGCTGGTA TGACTATGTG GAGGTGCGGG ATGGCTTCTG GAGAAAGGCG CCCCTCCGAG 
     2221 GCCGGTTCTG TGGGGGGAAA CTCCCCGAGC CCATTGTCTC CACCGACAGC CGCCTCTGGG 
     2281 TGGAATTCCG AAGCAGCAGC AATTGGGTTG GAAAGGGCTT CTTTGCTGTC TACGAAGCCA 
     2341 TTTGCGGTGG TGACGTGAAA AAGGATAACG GTCACATCCA GTCACCCAAT TACCCCGACG 
     2401 ATTACCGGCC CAGCAAAGTC TGCATCTGGC GGATCCAGGT GTCTGAGGGT TTCCACGTGG 
     2461 GCCTCACGTT CCAGTCCTTT GAGATCGAGC GTCACGACAG TTGTGCCTAC GACTACCTGG 
     2521 AGGTCCGAGA TGGGCACAGT GAGAGCAGCA ACCTCATTGG GCGATATTGT GGGTATGAGA 
     2581 AGCCTGATGA CATCAAAAGC ACATCTAGTC GGCTCTGGCT CAAGTTCGTC TCCGATGGGT 
     2641 CCATTAACAA AGCTGGCTTT GCAGTCAACT TTTTCAAAGA GGTAGATGAG TGTTCAAGGC 
     2701 CCAACCGCGG GGGCTGTGAG CAGCGGTGCC TAAACACCCT GGGCAGCTAC AAGTGCAGCT 
     2761 GTGACCCCGG CTATGAGCTG GCCCCAGACA AGCGTCGGTG TGAAGCTGCC TGCGGTGGAT 
     2821 TCCTCACCAA GCTCAATGGC TCCATCACCA GCCCCGGTTG GCCCAAAGAG TACCCTCCCA 
     2881 ACAAAAACTG CATCTGGCAG TTGGTGGCCC CCACCCAGTA CCGTATCTCC CTGCAATTTG 
     2941 ACTTCTTCGA GACTGAGGGC AATGATGTGT GCAAGTATGA TTTCGTGGAG GTGCGCAGCG 
     3001 GACTCACAGC CGACTCTAAA CTACATGGCA AGTTCTGTGG CTCCGAGAAA CCAGAGGTCA 
     3061 TCACTTCCCA GTACAACAAC ATGCGTGTGG AGTTCAAGTC TGACAATACT GTGTCCAAAA 
     3121 AGGGCTTCAA GGCCCACTTC TTCTCAGACA AGGATGAATG TTCCAAGGAC AATGGTGGCT 
     3181 GCCAGCAAGA CTGCGTGAAC ACATTTGGCA GCTACGAGTG TCAGTGTCGC AGCGGCTTTG 
     3241 TCCTTCACGA CAACAAACAT GACTGTAAGG AAGCCGGCTG TGAGCACAAA GTGACATCCA 
     3301 CCAGTGGCAC CATCACCAGC CCCAACTGGC CTGACAAGTA CCCCAGCAAG AAGGAGTGTA 
     3361 CGTGGGCTAT CTCCAGCACC CCTGGGCACC GGGTGAAGCT GACTTTTGTG GAGATGGATA 
     3421 TTGAGTCTCA GCCTGAATGC GCTTATGACC ACCTGGAGGT GTTTGATGGG CGTGATGCCA 
     3481 AGGCGCCAGT CCTTGGCCGA TTCTGTGGCA GTAAGAAGCC TGAGCCAGTC CTGGCTACCG 
     3541 GCAACCGCAT GTTCTTGCGC TTCTACTCAG ACAACTCAGT ACAGAGGAAA GGTTTCCAGG 
     3601 CCTCCCACTC CACAGAGTGT GGGGGCCAAG TGCGGGCAGA TGTGAAGACC AAAGACCTTT 
     3661 ATTCCCATGC CCAGTTTGGT GATAACAACT ACCCCGGAGG GGTGGACTGC GAGTGGGTGA 
     3721 TTGTGGCAGA GGAAGGCTAT GGCGTGGAGT TGGTGTTCCA GACCTTCGAG GTAGAGGAGG 
     3781 AGACCGACTG TGGCTATGAC TACATGGAGC TCTTCGACGG CTACGACAGC ACAGCTCCTA 
     3841 GACTGGGGCG CTACTGTGGC TCTGGGCCTC CTGAGGAAGT GTACTCGGCC GGAGACTCTG 
     3901 TCCTGGTGAA GTTCCACTCT GATGACACCA TCTCCAAGAA AGGCTTTCAC CTGCGGTACA 
     3961 CAAGCACCAA GTTCCAGGAC ACACTCCACA GCAGGAAGAC GCGTACGCGG CCGCTCGAGC 
     4021 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4081 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4141 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4201 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4261 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4321 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4381 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4441 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4501 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4561 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4621 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4681 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4741 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4801 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4861 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4921 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4981 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5041 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5101 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5161 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5221 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5281 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5341 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5401 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5461 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5521 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5581 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5641 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5701 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5761 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5821 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5881 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5941 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6001 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6061 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6121 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6181 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6241 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6301 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6361 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6421 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6481 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6541 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6601 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6661 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6721 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6781 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6841 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6901 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6961 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7021 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7081 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7141 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7201 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7261 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7321 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7381 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7441 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7501 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7561 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7621 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7681 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7741 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7801 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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