LOCUS       RR217384                7821 bp    DNA     circular  SYN 
DEFINITION  Stk10 (myc-DDK-tagged) - Rat serine/threonine kinase 10 (Stk10)
ACCESSION   NM_019206
VERSION     NM_019206.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3978..4069 
                     /label="Myc-DDK"  
     misc_feature    4099..4684 
                     /label="PolyA signal"  
     misc_feature    4156..4175 
                     /label="Restriction Enzyme"  
     misc_feature    4960..5632 
                     /label="ColE1"  
     misc_feature    6062..6856 
                     /label="Kan/Neo"  
     misc_feature    6891..7193 
                     /label="SV40 ori"  
     misc_feature    7279..7756 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3960..3966 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3960 
                     /label="Stk10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTCGCC AATTTTCGCC GCATCTTGCG GTTATCCACC TTCGAGAAGA 
     1081 GAAAGTCCCG TGAATACGAG CATGTCCGCA GGGACCTGGA CCCCAACGAC GTATGGGAGA 
     1141 TCCTGGGCGA GCTGGGAGAC GGCGCCTTCG GCAAAGTCTA CAAGGCCAAA AACAAGGAGA 
     1201 CGGGCGCCCT GGCAGCAGCC AAAGTCATTG AGACTAAGAG CGAGGAGGAG CTGGAGGACT 
     1261 ACATCGTGGA GATCGAGATC CTCGCCACCT GCGACCACCC ATATATCGTG AAGCTCCTGG 
     1321 GGGCCTATTA CTATGACGGG AAACTATGGA TCATGATTGA GTTCTGCCCT GGGGGAGCTG 
     1381 TGGATGCCAT CATGCTAGAG CTGGATCGAG GCCTCACTGA GCCCCAGATT CAGGTCGTTT 
     1441 GCCGCCAGAT GTTGGAGGCT CTCAACTTCC TGCACGGCAA GAGAATCATC CACCGCGACC 
     1501 TGAAAGCTGG CAACGTGCTT ATGACCCTGG AGGGGGACAT CAGGCTGGCT GATTTTGGAG 
     1561 TATCTGCCAA GAATCTGAAA ACTCTGCAAA AGCGAGATTC CTTCATAGGA ACGCCTTACT 
     1621 GGATGGCCCC TGAGGTGGTG CTGTGTGAAA CCATGAAGGA CGCCCCCTAT GACTACAAGG 
     1681 CTGACATCTG GTCCCTGGGC ATCACGCTGA TCGAGATGGC GCAGATTGAG CCCCCACACC 
     1741 ATGAGCTCAA CCCCATGCGG GTCCTGCTCA AGATCGCCAA GTCGGACCCT CCCACCCTGC 
     1801 TCACACCCTC CAAGTGGTCC ACGGAGTTCC GAGACTTTCT GAAGATAGCC TTGGATAAGA 
     1861 ACCCAGAAAC CCGGCCCAGT GCTGCGCAGC TGCTGCAGCA TCCCTTCGTC AGTACAGTCA 
     1921 CCAGTAATAA GGCCCTTCGG GAGCTGGTGG CTGAGGCGAA GGCTGAGGTG ATGGAGGAGA 
     1981 TCGAGGACGG CAAGGAGGAC GGCAGGGAGG ACGGCAGGGA GGATGGAGAA GAGGAGGATG 
     2041 GAGACGAGAA GGATGCTGTG AGTGCTGTTC CGCCCCCAGT CAACCACACT CAGGACTCCT 
     2101 CTGCCAATGG AACTCAGCCA AGCCTCAACT CTGACAAGCT TCTCCAGGAT TCTTCTACCC 
     2161 CCCTGCCTCC CAGCCAGCCT CAGGAGCCTG TGAACGGGCC CTGTAACCAA CCCTCAGGGG 
     2221 ATGGATCCCC CCAGAACACC AGCCCTGCAG ATGAGGTCTC CAAGAATGAC AATGGCTTAA 
     2281 AGGTACCTGT TCCCCTCCGG AAGTCCCGGC CATTGTCCGT GGATGCCAGA ATTCAGGTGA 
     2341 CCGAAGAGAA ACAAATCACT GACCAGGCTG AGAACCCCAG TTCTGCAGCC AGCAAACCCC 
     2401 CGAAGGTCAA CCAGAGCCGA CCTAACAGCA GCGCCCTGGA GACTTTGGGT GTCGAGACTC 
     2461 TGGCCAATGG AGGCCTGGAG CTCCCTGGCT CTGTAACTCC AAACCATTCT AAGAGGGCGT 
     2521 CGGACTGTAG CAACCTGTCT ACCTCAGAGA GCATGGACTA CGGCACCTCC TTGTCTGCTG 
     2581 ACCTGTCATT GAACAAAGAG ACGGGCTCAT TGTCTCTCAA GGGCTCAAAA CTGCACAACA 
     2641 AGACCCTGAA GAGGACCCGC CGGTTTGTGG TGGACGGTGT GGAGGTGAGC ATCACCACCT 
     2701 CCAAGATCAT CAGCGAAGAC GAGAAGAAAG ACGAGGAGAT GAGGTTTCTC AGGCGCCAGG 
     2761 AACTCCGAGA GCTTCGGCTC CTGCAGAAAG AAGAGCATCG GAACCAGACG CAGCTGAGCA 
     2821 CCAAGCACGA GCTGCAGCTG GAGCAGATGC ACAGACGATT TGAACAAGAA ATCAACGCCA 
     2881 AGAAGAAATT CTATGACGTG GAGCTAGAGA ACCTGGAGCG GCAGCAGAAG CAGCAGGTGG 
     2941 AGAAGATGGA GCAGGACCAC AGCGTGCGTC GCAGAGAGGA GGCCAAGCGG ATCCGCCTGG 
     3001 AGCAGGATCG AGACTACGCC AGGTTCCAAG AGCAGCTCAA GCAGATGAAG AAGGAGGTGA 
     3061 AGCTGACTGT CGAGAGGTTG GCTAGAAATA GTAGAAATAG TTCTTCCTTC CAGGAACAGA 
     3121 CTGAGCATCC TCAGGCAGCT AGGAGGAGGG ACCGAGACTT TGTAGCCAAG CAGAAGGAAG 
     3181 ACCTGGAGCT GGCCATGAAG AAGCTCACGG CAGAAAACAG GCGTGAGATC TGTGACAAGG 
     3241 AACGTGATTG CCTTAACAAG AAGCAGGAGC TCCTCCGAGA CCGAGAGGCA GCCCTGTGGG 
     3301 AGATGGAGGA GCACCAGTTA CAGGAGAGAC ATCAGCTGGT GAAGCAGCAG CTTAAGGACC 
     3361 AGTACTTCCT GCAGCGGCAT GACCTGCTGC GCAAGCACGA GAAGGAGCGG GAGCAGATGC 
     3421 AGCGCTACAA CCAGCGTATG ATGGAGCAGC TGAAGGTCAG ACAGCAGCAG GAGAAGGCGC 
     3481 GGCTACCCAA GATCCAGAGG AGTGACGGCA AGACCCGCAT GGCCATGTAC AAGAAGAGCC 
     3541 TGCACATCAA TGGTGCGGGC AGTGCCTCCG AGCAGCGGGA GAAGGTCAAG CAGTTCTCCC 
     3601 AGCAGGAAGA GAAGAGGCAG AAGGCGGAGA GGCTGCAGCA GCAGCAGAAA CACGAGAACC 
     3661 AGATGCGAGA CATGGTGGCA CAGTGCGAGA GCAACATGAA CGAGCTGCAG CAGCTGCAGA 
     3721 ATGAAAAGTG TCATCTGTTA GTGGAGCATG AAACCCAGAA GCTGAAGGCC CTGGACGAGA 
     3781 GCCATAACCA GAGCCTGAAG GAATGGCGAG ACAAGCTTCG GCCACGCAAA AAGGCCCTGG 
     3841 AAGAGGATTT GAACCAGAAG AAGCGGGAAC AGGAAATGTT CTTCAGACTA AGTGAGGAGG 
     3901 CAGAGACCAG ACCCACCACA CCCAACAGAG CCAGCAAGTT CTTCCCCTAC AGCTCTGGGG 
     3961 ATGCTTCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4021 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4081 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4141 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4201 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4261 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4321 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4381 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4441 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4501 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4561 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4621 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4681 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4741 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4801 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4861 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4921 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4981 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5041 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5101 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5161 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5221 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5281 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5341 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5401 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5461 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5521 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5581 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5641 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5701 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5761 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5821 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5881 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5941 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6001 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6061 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6121 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6181 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6241 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6301 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6361 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6421 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6481 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6541 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6601 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6661 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6721 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6781 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6841 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6901 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6961 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7021 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7081 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7141 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7201 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7261 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7321 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7381 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7441 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7501 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7561 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7621 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7681 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7741 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7801 CAAAAATTTA ACGCGAATTT T
//