LOCUS       RR217383                7812 bp    DNA     circular  SYN 
DEFINITION  Grik5 (myc-DDK-tagged) - Rat glutamate receptor, ionotropic, kainate 5 (Grik5)
ACCESSION   NM_031508
VERSION     NM_031508.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3967..4058 
                     /label="Myc-DDK"  
     misc_feature    4088..4673 
                     /label="PolyA signal"  
     misc_feature    4145..4164 
                     /label="Restriction Enzyme"  
     misc_feature    4949..5621 
                     /label="ColE1"  
     misc_feature    6051..6845 
                     /label="Kan/Neo"  
     misc_feature    6880..7182 
                     /label="SV40 ori"  
     misc_feature    7268..7745 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3957..3965 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3957 
                     /label="Grik5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGCTGAG CTGCTGCTGC TGCTGATAGT CGCCTTCGCC AACCCCAGCT 
     1081 GCCAGGTGCT GTCATCACTG CGCATGGCTG CAATCCTGGA CGACCAGACC GTGTGTGGCC 
     1141 GTGGTGAGCG TCTGGCCCTG GCCCTGGCCC GAGAGCAGAT CAATGGGATC ATCGAGGTCC 
     1201 CAGCCAAGGC CCGAGTGGAA GTAGACATCT TTGAGCTGCA ACGGGACAGC CAGTACGAGA 
     1261 CTACGGACAC CATGTGTCAG ATCCTGCCCA AGGGGGTTGT GTCTGTCTTG GGACCCTCCT 
     1321 CCAGCCCAGC TTCTGCCTCC ACCGTGAGCC ATATCTGTGG GGAGAAGGAG ATTCCCCACA 
     1381 TCAAGGTAGG CCCTGAGGAG ACACCCCGCC TTCAGTACCT CCGCTTTGCA TCTGTCAGCC 
     1441 TGTATCCCAG TAATGAAGAT GTCAGCCTGG CAGTCTCCCG GATCCTCAAG TCCTTTAACT 
     1501 ACCCCTCCGC TAGCCTCATC TGCGCCAAAG CTGAGTGCCT GCTGCGGTTA GAAGAACTGG 
     1561 TGCGAGGCTT CCTCATCTCC AAGGAGACAC TGTCCGTGAG GATGCTTGAT GACAGCCGGG 
     1621 ACCCCACGCC GCTACTCAAG GAGATCCGAG ATGACAAAGT GTCCACCATC ATCATCGATG 
     1681 CCAACGCGTC CATCTCCCAC CTTGTCCTCC GTAAGGCTTC GGAGCTGGGA ATGACCTCAG 
     1741 CGTTTTACAA GTACATCCTC ACCACCATGG ACTTCCCCAT CCTGCATCTG GACGGTATCG 
     1801 TAGAGGACTC CTCCAACATC TTGGGCTTTT CCATGTTCAA CACCTCCCAT CCCTTCTACC 
     1861 CGGAGTTTGT GCGCAGCCTC AACATGTCCT GGAGGGAGAA CTGCGAAGCC AGCACCTACC 
     1921 CTGGCCCTGC GCTGTCGGCA GCCCTGATGT TTGATGCCGT GCATGTGGTG GTAAGCGCTG 
     1981 TCCGAGAGCT GAACCGGAGC CAGGAGATTG GTGTCAAGCC ACTGGCTTGC ACTTCGGCCA 
     2041 ACATCTGGCC CCATGGGACC AGCCTCATGA ACTACCTGCG CATGGTAGAG TATGACGGGC 
     2101 TGACTGGGCG GGTTGAGTTC AACAGCAAAG GGCAGAGGAC CAACTACACA CTGCGCATCC 
     2161 TGGAGAAGTC CCGCCAGGGC CACCGTGAGA TAGGGGTGTG GTACTCTAAC CGGACCCTGG 
     2221 CCATGAATGC TACCACCCTG GACATCAACC TGTCACAGAC TCTAGCCAAC AAGACTCTGG 
     2281 TGGTCACAAC AATCCTGGAG AACCCGTATG TCATGCGCCG GCCCAACTTC CAGGCCCTGT 
     2341 CAGGGAACGA GCGCTTTGAG GGCTTCTGCG TGGACATGCT TCGGGAGCTG GCCGAGCTGC 
     2401 TGCGCTTCCG GTATCGCCTG CGGTTGGTGG AGGACGGACT GTACGGGGCA CCTGAGCCCA 
     2461 ATGGTTCCTG GACAGGAATG GTTGGCGAGC TCATCAACCG GAAGGCAGAC CTGGCTGTGG 
     2521 CAGCCTTCAC CATCACCGCG GAGAGAGAGA AGGTCATCGA CTTCTCCAAG CCATTCATGA 
     2581 CCCTGGGGAT CAGCATCCTC TACAGGGTGC ACATGGGCCG CAAGCCTGGC TACTTCTCCT 
     2641 TCCTGGACCC TTTCTCCCCT GCCGTGTGGC TCTTCATGCT TCTTGCCTAC CTGGCTGTCA 
     2701 GCTGTGTCTT GTTTCTGGCT GCCAGGCTGA GCCCTTATGA GTGGTATAAC CCTCACCCGT 
     2761 GTCTTCGGGC GCGTCCCCAT ATCCTGGAGA ACCAGTACAC GCTGGGTAAC AGCCTTTGGT 
     2821 TCCCTGTGGG TGGCTTCATG CAGCAGGGCT CGGAGATCAT GCCCCGGGCG CTGTCCACAC 
     2881 GCTGTGTCAG CGGAGTCTGG TGGGCCTTCA CCTTGATCAT CATCTCCTCC TACACGGCCA 
     2941 ACCTGGCTGC CTTCCTCACG GTGCAGCGCA TGGAGGTGCC GGTGGAGTCT GCAGACGACT 
     3001 TGGCGGATCA GACCAACATT GAGTACGGCA CTATCCACGC TGGCTCCACC ATGACCTTCT 
     3061 TTCAGAACTC ACGGTACCAG ACGTACCAGC GGATGTGGAA TTACATGCAG TCGAAGCAGC 
     3121 CCAGCGTGTT CGTCAAGAGC ACAGAGGAGG GGATCGCCCG TGTCCTCAAC TCACGTTACG 
     3181 CCTTCCTGCT GGAGTCCACC ATGAACGAGT ACCACAGGCG CCTCAATTGC AACCTCACCC 
     3241 AGATCGGGGG CCTCCTCGAC ACCAAGGGCT ACGGCATCGG CATGCCGCTG GGCTCCCCAT 
     3301 TCCGGGATGA GATCACGCTG GCCATCCTGC AGCTCCAGGA GAACAACAGG CTGGAGATCC 
     3361 TGAAGCGCAA GTGGTGGGAG GGCGGCCGGT GCCCCAAGGA GGAGGACCAC AGGGCCAAAG 
     3421 GTTTGGGCAT GGAGAACATT GGCGGTATTT TTGTCGTGCT GATCTGTGGC CTCATCATTG 
     3481 CTGTCTTCGT GGCGGTCATG GAATTCATCT GGTCCACGCG GAGGTCAGCG GAGTCTGAGG 
     3541 AGGTGTCGGT GTGCCAGGAG ATGCTGCAGG AGCTGCGCCA CGCCGTGTCT TGCCGCAAGA 
     3601 CCTCGCGTTC CCGCCGGCGC CGGCGCCCTG GCGGCCCGAG CCGGGCCCTG CTGTCGCTGC 
     3661 GCGCGGTCCG CGAGATGCGA CTCAGCAACG GCAAGCTCTA CTCGGCCGGC GCGGGCGGGG 
     3721 ACGCGGGCGC GCACGGGGGT CCGCAGCGCC TCCTGGACGA CCCCGGGCCT CCTGGGGGAC 
     3781 CCCGGCCCCA GGCTCCCACG CCCTGCACGC ACGTGCGCGT CTGCCAGGAG TGCAGGCGCA 
     3841 TCCAGGCGCT GCGAGCTTCA GGGGCCGGGG CGCCCCCGCG TGGCCTGGGC ACTCCGGCCG 
     3901 AAGCCACCAG CCCGCCTCGG CCGCGGCCTG GACCCACTGG ACCCCGCGAG CTGACTGAGC 
     3961 ATGAAACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4021 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4081 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4141 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4201 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4261 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4321 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4381 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4441 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4501 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4561 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4621 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4681 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4741 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4801 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4861 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4921 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4981 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5041 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5101 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5161 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5221 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5281 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5341 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5401 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5461 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5521 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5581 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5641 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5701 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5761 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5821 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5881 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5941 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6001 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6061 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6121 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6181 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6241 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6301 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6361 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6421 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6481 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6541 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6601 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6661 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6721 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6781 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6841 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6901 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6961 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7021 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7081 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7141 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7201 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7261 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7321 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7381 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7441 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7501 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7561 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7621 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7681 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7741 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7801 AACGCGAATT TT
//