LOCUS       RR217379                7806 bp    DNA     circular  SYN 
DEFINITION  Nfkb1 (myc-DDK-tagged) - Rat nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (Nfkb1)
ACCESSION   NM_001276711
VERSION     NM_001276711.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3961..4052 
                     /label="Myc-DDK"  
     misc_feature    4082..4667 
                     /label="PolyA signal"  
     misc_feature    4139..4158 
                     /label="Restriction Enzyme"  
     misc_feature    4943..5615 
                     /label="ColE1"  
     misc_feature    6045..6839 
                     /label="Kan/Neo"  
     misc_feature    6874..7176 
                     /label="SV40 ori"  
     misc_feature    7262..7739 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3936..3943 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3936 
                     /label="Nfkb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGACGAC GATCCTTTCG GAACTGGGCA AATGTTTCAT TTGAATACTG 
     1081 CTTTGACTCA CTCCATATTT AATGCAGAAT TATATTCAAC AGATATACCA CTGTCAACAG 
     1141 ATGGCCCATA CCTTCAAATA CTAGAGCAAC CGAAACAGAG AGGATTTCGA TTCCGCTACG 
     1201 TGTGCGAAGG CCCGTCCCAC GGAGGGCTTC CCGGTGCCTC CAGTGAGAAG AACAAGAAAT 
     1261 CTTACCCGCA GGTCAAAATT TGCAACTATG TGGGGCCTGC AAAGGTTATC GTTCAGTTGG 
     1321 TCACAAATGG AAAAAATATT CACCTGCACG CCCACAGCCT GGTGGGCAAG CACTGTGAGG 
     1381 ACGGCATATG CACCGTGACA GCAGGACCCA AGGACATGGT GGTTGGCTTT GCAAACCTGG 
     1441 GAATACTTCA CGTGACTAAG AAAAAGGTAT TCGAAACACT GGAAGCACGG ATGACAGAGG 
     1501 CGTGTATAAG GGGCTATAAC CCTGGACTTC TGGTGCATTC TGACCTTGCC TATCTACAGG 
     1561 CAGAAGGCGG AGGAGACCGG CAACTCACAG ACAGAGAGAA GGAGATCATC CGCCAGGCAG 
     1621 CTCTGCAGCA GACCAAGGAG ATGGACCTGA GTGTGGTGCG CCTCATGTTC ACAGCCTTCC 
     1681 TCCCCGACAG CACGGGCAGC TTCACTCGGA GACTGGAACC TGTGGTGTCA GACGCCATCT 
     1741 ATGACAGCAA AGCCCCCAAC GCGTCCAACC TGAAGATCGT AAGAATGGAC AGAACAGCAG 
     1801 GCTGCGTGAC GGGAGGGGAA GAAATCTATC TTCTCTGTGA CAAGGTCCAG AAAGATGACA 
     1861 TCCAGATTCG GTTTTATGAA GAGGAAGAAA ATGGTGGAGT TTGGGAAGGA TTTGGGGACT 
     1921 TTTCCCCCAC GGATGTTCAC AGACAGTTTG CCATTGTCTT CAAAACACCG AAGTATAAGG 
     1981 ATGTCAACAT TACAAAGCCA GCTTCCGTGT TTGTTCAGCT TCGGAGAAAA TCGGATTTGG 
     2041 AAACTAGTGA ACCGAAACCC TTTCTCTACT ACCCCGAAAT CAAAGACAAG GAGGAAGTAC 
     2101 AGAGGAAACG CCAGAAGCTC ATGCCCAACT TCTCCGACAG TTTCGGCGGC GGCAGTGGAG 
     2161 CGGGAGCGGG TGGCGGAGGC ATGTTCGGTA GTGGCGGTGG CGGTGGAGGG AGTACCGGAA 
     2221 GTCCTGGCCC AGGGTATGGC TTCCCGCACT ATGGATTTCC TGCTTACGGT GGGATTGCAT 
     2281 TCCATCCCGG AGCCACTAAA TCCAACACAG GCATCACCCA TGGCACCATA AACACCAAAT 
     2341 TTAAAAACGA GCCTAGAGAT TGTGCCAAGA GTGACGACAG GGAGATTCTG AATCCCCCTG 
     2401 AGAAAGAAAC ACAAGGTGAG GGGCCCAGCC TGTTCATGGC CAGCACCAAG ACCGAAGCAA 
     2461 TTGCCCCGGC ATCCACCATG GAAGACAAGG AAGAGGATGT GGGGTTTCAG GATAACCTCT 
     2521 TTCTTGAGAA GGCGCTGCAG CTCGCCAAGC GGCATGCCAA CGCCCTCTTC GACTACGCGG 
     2581 TTACGGGAGA TGTGAAGATG CTGCTGGCGG TACAGCGCCA TCTCACCGCG GTGCAGGATG 
     2641 AGAACGGGGA CAGTGTCCTA CACTTAGCCA TCATCCACCT TCATGCTCAG CTCGTGAGGG 
     2701 ATCTGCTAGA AGTGACATCT GGTTCGATCT CTGATGACAT CATCAACATG AGAAACGATC 
     2761 TGTATCAGAC ACCTTTGCAC TTGGCCGTGA TCACCAAGCA GGAAGATGTG GTGGAGGACT 
     2821 TGCTGAGGGT TGGGGCTGAC CTGAGTCTTC TGGACCGCTG GGGCAACTCT GTCCTGCACC 
     2881 TAGCTGCCAA AGAAGGACAT GATAAAATCC TCGGGGTCCT ACTCAAGAAC AGCAAGGCAG 
     2941 CACTCCTTAT CAACCACCCC AACGGGGAAG GTCTGAATGC CATTCACATA GCTGTGATGA 
     3001 GCAACAGCCT GTCATGTCTG CAGCTGCTTG TGGCTGCTGG GGCAGAAGTC AACGCTCAGG 
     3061 AGCAGAAGTC TGGGCGCACG GCACTGCACC TGGCCGTGGA GTACGACAAC ATCTCGTTGG 
     3121 CCGGCTGCCT GCTCCTGGAG GGTGACGCCC TCGTGGACAG CACCACCTAC GATGGGACGA 
     3181 CACCTCTACA CATAGCAGCT GGAAGAGGGT CCACCAGGCT GGCAGCTCTT CTCAAAGCAG 
     3241 CAGGAGCAGA CCCCCTGGTG GAGAACTTTG AGCCTCTCTA TGACCTGGAC GACTCTTGGG 
     3301 AGAAGGCTGG AGAAGATGAG GGAGTAGTGC CAGGCACCAC ACCCCTGGAC ATGGCTGCCA 
     3361 ACTGGCAGGT ATTTGACATA CTAAATGGGA AACCATATGA GCCAGTGTTC ACATCTGATG 
     3421 ACATACTCCC ACAAGGGGAC ATTAAGCAGC TGACAGAAGA CACGAGGCTG CAACTCTGCA 
     3481 AGCTCTTGGA AATTCCTGAT CCCGACAAGA ACTGGGCCAC TCTGGCGCAG AAGTTAGGTC 
     3541 TGGGGATATT GAACAATGCC TTCCGGCTGA GTCCCGCCCC TTCTAAAACT CTTATGGACA 
     3601 ACTATGAGGT CTCTGGGGGG ACCATCAAAG AGCTGGTGGA GGCCCTGCGA CAGATGGGCT 
     3661 ACACAGAGGC CATTGAAGTG ATCCAGGCAG CCTTCCGCAC CCCGGAAACC ACAGCCTCCA 
     3721 GCCCCGTGAC CACTGCTCAG GCCCACTTGC TGCCTCTCTC GTCCTCCTCC ACAAGACAGC 
     3781 ACATAGATGA GCTCCGAGAT AATGACAGCG TGTGTGACAG TGGTGTGGAG ACATCCTTCC 
     3841 GCAAACTCAG CTTCTCGGAG TCCCTCACTG GAGATGGCCC ACTGCTATCT CTGAACAAAA 
     3901 TGCCTCACAA TTATGGGCAG GATGGACCTA TAGAAGGCAA AATTAGCGGA CCGACGCGTA 
     3961 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4021 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4081 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4141 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4201 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4261 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4321 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4381 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4441 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4501 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4561 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4621 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4681 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4741 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4801 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4861 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4921 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4981 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5041 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5101 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5161 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5221 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5281 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5341 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5401 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5461 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5521 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5581 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5641 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5701 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5761 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5821 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5881 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5941 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6001 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6061 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6121 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6181 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6241 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6301 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6361 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6421 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6481 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6541 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6601 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6661 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6721 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6781 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6841 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6901 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6961 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7021 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7081 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7141 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7201 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7261 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7321 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7381 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7441 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7501 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7561 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7621 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7681 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7741 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7801 AATTTT
//