LOCUS       RR217374                7788 bp    DNA     circular  SYN 
DEFINITION  Pkd2 (myc-DDK-tagged) - Rat polycystic kidney disease 2 (autosomal dominant) (Pkd2)
ACCESSION   NM_001191934
VERSION     NM_001191934.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3943..4034 
                     /label="Myc-DDK"  
     misc_feature    4064..4649 
                     /label="PolyA signal"  
     misc_feature    4121..4140 
                     /label="Restriction Enzyme"  
     misc_feature    4925..5597 
                     /label="ColE1"  
     misc_feature    6027..6821 
                     /label="Kan/Neo"  
     misc_feature    6856..7158 
                     /label="SV40 ori"  
     misc_feature    7244..7721 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3918..3925 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3918 
                     /label="Pkd2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGAACTCC AGCCGTGTGC AGCCGCAGCC TCCCGGGGAC CCCGGACGCT 
     1081 CGCCCGCGCC GCGAGCGTCC GGGCCCGGCC GCCTGCTGGC GGGAGGCGCC GGCCTGGCAG 
     1141 TCCCCGGCGG CCTGCGGGAG CAGCGGGGCC TGGAGATTGA GATGGAGCGC ATCCGGCAGG 
     1201 CGGCCGCTCG GGACCCACCG GCCGGAGCCT CGGCCTCGCC TTCTCCTCCG CTGTCGTCCT 
     1261 GCTCCAGGCA GGCATGGAGC CGCGACAACC CGGGCTTTGA GGCCGAAGAG GATGACGACG 
     1321 ACGACGAGGT GGAAGGAGAA GAAGGAGGGA TGGTGGTGGA GATGGACGTG GAGTGGCGTC 
     1381 CGGGCAGCCG AAGACCGGCC TCCTCTTCGG CCGTGAGCTC GGCGGGCGCC CGCGGCCGGG 
     1441 GTCTGGGAAG CTATCGCGGC GCGGCTTACC CGAGCGGGAG ACGGCGCCGG CTAGAGGACC 
     1501 AGGGCGCGCC GTGTCCCAGC CCCGCGGGCG GCGGGGACCC GCTGCATCGC CACCTCCCGC 
     1561 TGGATGGCCA GCCGCCCCGA GTGGCCTGGG CCGAGAGGCT GGTGCGAGGA CTGCGAGGTC 
     1621 TCTGGGGAAC AAGACTCATG GAAGAGAGCA ACGCTAACCG AGAGAAATAC CTGAGAAGCG 
     1681 TGTTGCGGGA GCTGGTCACT TACCTCTTTT TCCTCATACT CTTGTGCATC TTGACCTATG 
     1741 GCATGATGAG CTCCAACGTG TACTACTACA CTCGGACACT GTCACAACTT TTCATAGACA 
     1801 CCCCAGTGTC TAAAACAGAG AAAACCAACT TTAAAACTCT CTCTTCCATG GAGGACTTCT 
     1861 GGAAGTTCAC AGAAGGCTCC TTCCTGGATG GGCTGTACTG GAAGGCACAG AGCAGCAACC 
     1921 ATACGCAAGC TGACAACCGG AGCTTTATTT TCTATGAGAA CCTGCTGCTA GGAGTGCCGC 
     1981 GTCTACGCCA ACTCCGAGTC AGAAACGGAT CCTGCTCCAT CCCTCAGGAC CTGAGAGATG 
     2041 AAATTAAAGA ATGCTATGAT GTCTACTCTG TCAGCAGTGA GGACAGGGCT CCATTTGGAC 
     2101 CGCGGAACGG AACTGCGTGG ATCTACACAA GTGAAAAAGA CCTGAATGGC AGCAGTCACT 
     2161 GGGGAATCAT TGCGACTTAC AGTGGAGCCG GTTACTACCT GGATCTGTCC AGAACCAGGG 
     2221 AGGAGACAGC GGCCCAGCTT GCTGGCCTCA GGAGGAATTT CTGGCTGGAC CGGGGCACAC 
     2281 GGGCAGCTTT CATTGACTTC TCGGTGTATA ACGCAAACAT TAACCTGTTC TGTGTGGTCA 
     2341 GGTTATTGGT TGAGTTCCCA GCAACGGGTG GCGTGGTGCC CTCTTGGCAG TTTCAGCCTG 
     2401 TAAAACTGAT CCGCTACGTC ACAGCCTTTG ATTTCTTCCT GGCAGCCTGT GAGATTATCT 
     2461 TTTGTTTCTT TATCCTTTAC TATGTGGTGG AAGAGATATT GGAAATTCGG ATTCACAGAC 
     2521 TGAACTATTT CAGGAGTTTC TGGAATTGTC TGGATGTTGT GATTGTTGTG TTGTCCGTAG 
     2581 TAGCCATGAT GATTAACATT TACCGGATGT CCAATGCTGA GGGGCTGCTA CAGTTTCTCG 
     2641 AAGATCAAAA TTCTTTCCCC AACTTTGAGC ATGTGGCATA CTGGCAAATA CAGTTCAACA 
     2701 ATATAGCTGC CGTCATGGTA TTTTTGGTCT GGATTAAGCT CTTCAAATTC ATCAATTTCA 
     2761 ATAGGACCAT GAGTCAGCTC TCCACGACCA TGTCCAGATG TGCCAAAGAC CTCTTTGGCT 
     2821 TCACCATCAT GTTCTTCATC ATCTTCTTGG CATATGCCCA GTTGGCGTAC CTTGTCTTTG 
     2881 GCACTCAGGT GGATGACTTC AGTACTTTCC AAGAGTGTAT TTTCACTCAG TTCCGCATCA 
     2941 TTTTGGGTGA TATCAACTTC GCAGAGATCG AGGAAGCTAA CCGAGTTTTG GGGCCACTTT 
     3001 ATTTTACTAC ATTTGTGTTC TTTATGTTCT TCATTCTTTT GAACATGTTT CTGGCGATCA 
     3061 TCAATGATTC CTACTCTGAG GTGAAATCAG ATCTGGCCCA GCAGAAAGCA GAAATGGAAC 
     3121 TCTCAGACCT TATCAGAAAG GGCTGCCAAA AAGCACTTGT CAAACTAAAA CTTAAAAGAA 
     3181 ACACCGTAGA CGCCATTTCA GAGAGTCTCC GGCAAGGTGG TGGCAAGCTA AACTTTGATG 
     3241 AACTTCGGCA AGACCTGAAA GGGAAGGGCC ACACGGACGC AGAGATCGAG GCCATATTCA 
     3301 CTAAATATGA CCAGGACGGC GACCAGGAAC TGACTGAGCG TGAGCATCAG CAGATGAGAG 
     3361 ATGACTTGGA GAGAGAGAGG GAGGACCTAG ACTTGGAACA CAGCTCTTTA CCACGTCCCA 
     3421 TGAGCAGCAG AAGTTTCCCC AGAAGCCTGG ATGACTCTGA GGAGGAAGAC GATGAAGACA 
     3481 GTGGCCATAG TTCCAGGAGG AGGGGAAGCA TCTCCAGTGG GGTTTCCTAT GAAGAGTTCC 
     3541 AAGTACTGGT GAGACGCGTG GACCGCATGG AGCACTCCAT CGGCAGCATC GTGTCCAAGA 
     3601 TCGATGCTGT GATTGTCAAG CTTGAGATCA TGGAGCGGGC CAAGGTGAAG AGGCGAGAGG 
     3661 TGTTAGGAAG GCTGCTGGAT GGAGTGGCTG AGGATGCGCG CCTGGGTCGG GATAGTGAGA 
     3721 TCCACAGGGA GCAGATGGAG CGCCTGGTGC GGGAAGAGCT GGAGCGCTGG GAATCGGATG 
     3781 ACGCAGCTTC ACAGTCAGGT CACGGTCTAG GCACACAAGT GGGACTCGGT GGCCAGTCGC 
     3841 ACCCCAGAAG CTCCCGGCCC CCTTCCTCCC AGTCTGCAGA GGGCCTGGAA GGCGGAGGTG 
     3901 GAAACGGAAG TACCAACGCG CATGCGAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     3961 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4021 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4081 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4141 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4201 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4261 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4321 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4381 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4441 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4501 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4561 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4621 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4681 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4741 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4801 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4861 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4921 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4981 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5041 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5101 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5161 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5221 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5281 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5341 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5401 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5461 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5521 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5581 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5641 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5701 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5761 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5821 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5881 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5941 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6001 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6061 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6121 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6181 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6241 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6301 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6361 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6421 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6481 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6541 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6601 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6661 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6721 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6781 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6841 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6901 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6961 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7021 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7081 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7141 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7201 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7261 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7321 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7381 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7441 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7501 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7561 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7621 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7681 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7741 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//