LOCUS       RR217373                7776 bp    DNA     circular  SYN 
DEFINITION  Slc8a1 (myc-DDK-tagged) - Rat solute carrier family 8 (sodium/calcium exchanger), member 1 (Slc8a1), transcript variant 9
ACCESSION   NM_001270779
VERSION     NM_001270779.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3933..4024 
                     /label="Myc-DDK"  
     misc_feature    4054..4639 
                     /label="PolyA signal"  
     misc_feature    4111..4130 
                     /label="Restriction Enzyme"  
     misc_feature    4915..5587 
                     /label="ColE1"  
     misc_feature    6017..6811 
                     /label="Kan/Neo"  
     misc_feature    6846..7148 
                     /label="SV40 ori"  
     misc_feature    7234..7711 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3915..3921 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3915 
                     /label="Slc8a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTCGACTA AGTCTCCCAC CCAATGTTTC AATGGGATTT CGTCTGGTAA 
     1081 CTCTGGTGGC TCTCTTGTTT ACCCATGTTG ACCATATAAC TGCAGATACA GAGGCAGAAA 
     1141 CAGGAGGAAA TGAAACCACT GAATGTACTG GCTCATATTA CTGTAAGAAA GGGGTGATTC 
     1201 TGCCCATTTG GGAACCCCAA GACCCATCTT TTGGGGATAA AATTGCTAGA GCAACTGTGT 
     1261 ATTTTGTGGC CATGGTCTAC ATGTTCCTCG GAGTTTCTAT TATTGCCGAC CGGTTTATGT 
     1321 CCTCTATAGA AGTCATCACC TCTCAAGAAA AGGAGATTAC CATAAAGAAA CCAAATGGAG 
     1381 AGACCACCAA GACTACAGTG CGTATCTGGA ATGAGACTGT GTCCAACCTG ACCTTGATGG 
     1441 CCCTGGGATC TTCCGCTCCT GAGATTCTCC TGTCTGTCAT TGAAGTGTGT GGCCATAACT 
     1501 TCACCGCAGG GGACCTTGGT CCCAGCACCA TTGTGGGAAG CGCCGCCTTC AACATGTTCA 
     1561 TCATCATCGC GCTTTGTGTT TATGTGGTCC CAGATGGAGA GACGAGGAAG ATTAAACATC 
     1621 TGCGTGTGTT CTTTGTGACA GCAGCCTGGA GCATCTTTGC CTATACCTGG CTTTACATAA 
     1681 TTTTGTCTGT CAGCTCTCCT GGTGTCGTGG AGGTCTGGGA AGGATTACTC ACTTTCTTCT 
     1741 TCTTTCCCAT CTGTGTTGTG TTCGCTTGGG TTGCAGACAG GCGGCTTCTC TTTTACAAGT 
     1801 ATGTCTACAA GCGGTACAGG GCTGGCAAGC AGAGGGGGAT GATCATTGAA CATGAAGGAG 
     1861 ACAGACCAGC TTCCAAAACT GAAATTGAAA TGGATGGGAA AGTAGTCAAC TCCCACGTTG 
     1921 ACAATTTCTT AGATGGCGCT CTGGTTTTGG AAGTCGATGA GAGGGACCAA GATGACGAGG 
     1981 AAGCCAGGCG TGAGATGGCA AGGATTCTGA AGGAACTTAA GCAGAAGCAT CCCGACAAAG 
     2041 AGATCGAACA ATTAATAGAA TTAGCCAACT ATCAAGTCCT AAGTCAGCAG CAAAAGAGCC 
     2101 GAGCATTTTA CCGAATTCAA GCTACTCGCC TGATGACTGG AGCTGGTAAC ATTTTGAAGA 
     2161 GGCATGCAGC TGACCAAGCG AGGAAGGCTG TCAGCATGCA TGAAGTCAAC ATGGATGTGG 
     2221 TTGAAAATGA CCCAGTCAGT AAGGTCTTCT TTGAGCAAGG GACATACCAG TGTCTAGAAA 
     2281 ACTGTGGTAC TGTGGCCCTC ACCATTATTC GAAGAGGGGG TGACTTGACC AACACTGTGT 
     2341 TTGTTGACTT CAGGACGGAA GATGGCACAG CCAATGCTGG GTCTGATTAT GAGTTCACGG 
     2401 AAGGGACTGT GATCTTCAAA CCTGGGGAGA CCCAGAAGGA AATCAGAGTT GGCATCATTG 
     2461 ATGATGATAT CTTTGAAGAA GATGAAAACT TTCTTGTGCA TCTTAGCAAC GTCAGGGTCT 
     2521 CTTCAGAAGT CTCGGAAGAT GGCATACTAG ACTCCAATCA CGTGTCTGCG ATTGCTTGTC 
     2581 TCGGGTCACC CAACACTGCC ACCATAACCA TTTTTGATGA TGACCACGCG GGCATCTTTA 
     2641 CTTTCGAGGA ACCCGTGACT CACGTGAGCG AGAGCATTGG CATCATGGAG GTGAAGGTGC 
     2701 TGAGAACCTC TGGAGCGCGA GGAAATGTTA TCATTCCCTA TAAAACCATT GAAGGCACAG 
     2761 CCCGAGGTGG AGGGGAGGAC TTTGAGGACA CCTGTGGAGA GCTGGAATTC CAGAATGATG 
     2821 AAATAGTCAA AACAATATCA GTCAAGGTAA TCGATGACGA GGAGTATGAG AAAAACAAGA 
     2881 CCTTCTTCAT TGAGATTGGA GAACCCCGTC TGGTGGAGAT GAGTGAGAAG AAAGCCCTGT 
     2941 TGTTAAATGA GCTTGGTGGC TTCACATTAA CAGGCCAACC TGTCTTCAGG AAGGTCCATG 
     3001 CTAGAGATCA TCCGATTCCC TCTACCGTAA TCAGCATTTC AGAGGAGTAC GATGACAAGC 
     3061 AGCCACTGAC CAGCAAAGAG GAGGAGGAGA GGCGCATTGC AGAAATGGGG CGCCCCATTC 
     3121 TAGGCGAACA CACCAAGCTG GAAGTGATCA TTGAAGAGTC TTACGAATTC AAGAGCACTG 
     3181 TGGACAAACT CATTAAGAAG ACGAACCTGG CCCTCGTGGT GGGGACCAAC AGCTGGAGAG 
     3241 AGCAGTTCAT TGAAGCGATC ACCGTCAGCG CTGGGGAAGA TGACGATGAT GATGAATGTG 
     3301 GGGAGGAGAA GCTGCCCTCC TGTTTTGATT ACGTGATGCA CTTTCTCACA GTGTTCTGGA 
     3361 AGGTTCTGTT TGCCTTCGTC CCACCTACAG AATATTGGAA TGGCTGGGCC TGCTTCATTG 
     3421 TCTCCATCCT CATGATCGGC CTACTGACAG CCTTCATTGG AGATCTGGCT TCCCACTTTG 
     3481 GCTGCACCAT TGGTCTGAAA GATTCCGTGA CTGCAGTTGT GTTTGTCGCT CTTGGAACCT 
     3541 CAGTGCCAGA CACATTTGCC AGCAAAGTAG CAGCTACCCA GGACCAGTAT GCAGATGCGT 
     3601 CCATAGGCAA TGTCACCGGA AGCAACGCTG TGAATGTCTT CCTGGGAATC GGCGTGGCCT 
     3661 GGTCCATTGC TGCCATCTAC CATGCGGCCA ACGGGGAACA GTTCAAAGTG TCCCCTGGCA 
     3721 CGCTAGCTTT CTCTGTCACT CTCTTCACCA TTTTTGCTTT CATCAACGTG GGGGTGCTGC 
     3781 TGTATCGGCG GAGGCCAGAA ATAGGAGGTG AGCTGGGTGG GCCCCGGACT GCCAAGCTCC 
     3841 TCACATCTTC CCTGTTTGTG CTCCTGTGGC TCTTGTACAT TTTCTTCTCC TCCCTGGAGG 
     3901 CCTACTGCCA CATAAAAGGC TTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3961 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4021 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4081 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4141 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4201 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4261 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4321 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4381 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4441 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4501 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4561 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4621 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4681 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4741 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4801 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4861 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4921 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4981 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5041 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5101 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5161 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5221 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5281 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5341 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5401 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5461 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5521 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5581 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5641 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5701 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5761 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5821 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5881 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5941 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6001 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6061 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6121 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6181 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6241 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6301 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6361 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6421 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6481 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6541 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6601 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6661 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6721 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6781 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6841 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6901 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6961 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7021 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7081 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7141 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7201 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7261 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7321 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7381 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7441 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7501 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7561 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7621 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7681 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7741 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//