LOCUS       RR217365                7764 bp    DNA     circular  SYN 
DEFINITION  Thbs4 (myc-DDK-tagged) - Rat thrombospondin 4 (Thbs4)
ACCESSION   NM_017133
VERSION     NM_017133.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3921..4012 
                     /label="Myc-DDK"  
     misc_feature    4042..4627 
                     /label="PolyA signal"  
     misc_feature    4099..4118 
                     /label="Restriction Enzyme"  
     misc_feature    4903..5575 
                     /label="ColE1"  
     misc_feature    6005..6799 
                     /label="Kan/Neo"  
     misc_feature    6834..7136 
                     /label="SV40 ori"  
     misc_feature    7222..7699 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3903..3909 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3903 
                     /label="Thbs4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTAGCCCCA CGCGCGGCCG CCTTCCTCCT ACTGCACCTG GTCCTGCAGC 
     1081 CGTGGCAGCG GGCGGGCGCC CAGGCCACAC CCCAGGTCTT TGACCTTCTG CCATCCTCCA 
     1141 GCCAGAGGCT GAACCCGGCC GCCCTGCAGC CAGTCCTGAC AGACCCCACC CTGCACGAGC 
     1201 TCTATGTCAT CTCCACCTTC AAGCTGCAGA GTAAGAGCTC AGCCACCATC TTTGGCCTCT 
     1261 ACTCCTCGAG TGACAACAGC AAATACTTTG AATTCACTGT GATGGGACGC CTGAACAAAG 
     1321 CCATCCTCCG TTACCTAAAG GATGATGGGA AGATTCATCT GGTGGTTTTC AACAACCTGC 
     1381 AGCTGGCGGA TGGGAGGCGC CACCGGATTC TCCTGAGACT CAGCAATTTG CAGAGAGGGG 
     1441 CCGGCTCCGT AGAACTCTAC TTGGACTGTG TCCAGGTTGA TTCAGTGAAC AACCTCCCCA 
     1501 GAGCCTTCTC TGGTCTCACC CAGAACCCAC AGGCCATTGA ATTGAGAACA TTCCAGAGGA 
     1561 AGCCCCAGGA CTTCTTGGAA GAGCTGAAGC TGGTGGTGAG GGGCTCTCTG TTCCAGGTGG 
     1621 CCAGCCTACA AGACTGTTTC CTGCAGCAGA GTGAGCCGCT GGCTGCCACA GGCACAGGAG 
     1681 ACTTTAATCG ACAGTTTTTG GGTCAAATGA CACAATTAAA CCAGCTCCTT GGAGAGGTGA 
     1741 AAGATCTTCT GAGGCAGCAG GTCAAGGAAA CATCATTTTT GCGAAACACT ATTGCTGAGT 
     1801 GCCAGGCTTG TGGCCCTCTC AGCTTCCAGT CCCCAACCCC AAACACGCTG GTGCCCATAG 
     1861 CTCCTCCAGC ACCCCCAACC CGACCAACAC GTCGCTGTGA CTCCAGCCCT TGTTTCCGAG 
     1921 GTGTCCGATG CACGGACACC AGGGACGGCT TCCAATGTGG ACCCTGCCCA GATGGCTACA 
     1981 CGGGGAACGG GATCACCTGT TCTGACGTGG ATGAGTGTAA ATACCATCCC TGCTACCCTG 
     2041 GTGTGCGCTG CACAAATTTG GCTCCGGGTT TCAGATGTGA TGCCTGTCCA GTAGGCTTCA 
     2101 CGGGGCCCAT GGTGCAGGGC GTGGGGATCA ACTTTGCCAA AACAAACAAG CAGGTGTGCA 
     2161 CTGATGTTGA CGAGTGTCGG AACGGAGCGT GTGTTCTGAA CTCCATCTGC ATTAACACTC 
     2221 TGGGCTCTTA CCGCTGTGGG CCTTGCAAGC CAGGGTACAC TGGTGACCAG ACGAGGGGAT 
     2281 GCAGGACCGA AAGGAGCTGC CGGAACCCAG AGCAGAACCC GTGCAGTGTG CATGCGCAGT 
     2341 GCATTGAGGA ACGGCAAGGG GATGTGACGT GTGTGTGCGG TGTTGGTTGG GCCGGCGACG 
     2401 GCTATGTCTG TGGAAAGGAT GTGGACATCG ACAGTTACCC CGATGAAGAA CTGCCGTGCT 
     2461 CAGCCAGGAA CTGCAAGAAG GATAACTGCA AGTATGTGCC AAACTCCGGC CAAGAAGACG 
     2521 CAGACAGAGA TGGCATTGGA GACGCCTGTG ATGAGGATGC GGATGGAGAC GGGATCTTGA 
     2581 ATGAGCAGGA CAACTGTGTC CTGACTCACA ATGTGGACCA GAGGAACACT GATAAAGATA 
     2641 TCTTTGGGGA TGCCTGTGAC AACTGCCGGG GCGTCCTGAA TAATGACCAG AAGGACACTG 
     2701 ACGGGGATGG GAAAGGAGAC GCCTGTGATG ACGACATGGA TGGAGACGGA ATAAAAAACA 
     2761 TTTTGGACAA CTGTCCCCGA GTTCCCAACC GCGACCAACA GGATCGGGAT GGCGATGGTG 
     2821 TCGGAGACGC CTGTGACAGC TGTCCTGATG TCAGTAACCC TAACCAGTCG GATGTGGATA 
     2881 ACGATCTGGT TGGGGACTCC TGTGACACCA ACCAAGACAG TGATGGGGAT GGCCACCAGG 
     2941 ACAGCACAGA CAACTGCCCC ACCGTCATTA ACAGCGCCCA GCTGGACACC GACAAGGACG 
     3001 GGATTGGCGA TGAATGCGAT GACGATGACG ATAATGACGG CATCCCAGAC CTGGTTCCCC 
     3061 CTGGCCCAGA CAACTGCAGG CTTGTTCCCA ACCCAGCCCA GGAAGATAGC AACAATGATG 
     3121 GAGTAGGAGA TATCTGTGAA GCTGACTTCG ACCAGGACAA GGTCATTGAT CGCATTGATG 
     3181 TGTGTCCTGA GAATGCGGAG ATCACCCTGA CTGACTTCAG GGCCTATCAG ACTGTGGTCC 
     3241 TGGACCCTGA AGGGGATGCC CAGATCGACC CCAACTGGGT GGTCCTGAAC CAGGGCATGG 
     3301 AGATTGTGCA GACCATGAAC AGTGACCCTG GCCTAGCAGT CGGGTACACG GCATTTAATG 
     3361 GAGTTGACTT TGAAGGGACC TTCCACGTAA ACACCCAGAC AGATGACGAC TATGCCGGCT 
     3421 TTATTTTTGG TTACCAAGAC AGTTCCAGCT TCTATGTGGT CATGTGGAAA CAGACAGAGC 
     3481 AGACATACTG GCAGGCTACG CCATTCCGTG CAGTAGCAGA ACCTGGTATT CAGCTCAAGG 
     3541 CCGTGAAGTC TAAAACTGGC CCAGGGGAAC ATCTCCGAAA CTCCCTGTGG CACACAGGGG 
     3601 ACACCAGCGA CCAGGTCAGG CTGCTGTGGA AGGACTCCAG GAATGTGGGC TGGAAGGACA 
     3661 AGGTGTCCTA CCGCTGGTTC CTGCAGCATA GGCCCCAGGT GGGCTACATC AGGGTGCGGT 
     3721 TTTATGAAGG CTCAGAGTTG GTGGCTGACT CCGGGGTCAC TATAGACACC ACCATGCGCG 
     3781 GAGGCCGACT GGGCGTGTTC TGCTTCTCTC AGGAAAACAT CATCTGGTCC AACCTCAAGT 
     3841 ACCGCTGTAA CGACACCATA CCAGAGGACT TCCAGGAGTT CCAGATCCAG ACCTTTGACC 
     3901 GCCTGGATAA TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3961 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4021 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4081 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4141 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4201 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4261 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4321 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4381 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4441 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4501 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4561 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4621 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4681 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4741 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4801 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4861 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4921 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4981 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5041 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5101 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5161 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5221 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5281 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5341 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5401 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5461 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5521 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5581 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5641 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5701 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5761 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5821 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5881 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5941 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6001 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6061 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6121 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6181 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6241 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6301 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6361 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6421 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6481 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6541 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6601 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6661 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6721 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6781 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6841 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6901 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6961 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7021 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7081 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7141 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7201 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7261 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7321 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7381 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7441 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7501 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7561 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7621 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7681 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7741 TAACAAAAAT TTAACGCGAA TTTT
//