LOCUS       RR217356                7728 bp    DNA     circular  SYN 
DEFINITION  Iqsec2 (myc-DDK-tagged) - Rat IQ motif and Sec7 domain 2 (Iqsec2), transcript variant 2
ACCESSION   NM_001277386
VERSION     NM_001277386.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3885..3976 
                     /label="Myc-DDK"  
     misc_feature    4006..4591 
                     /label="PolyA signal"  
     misc_feature    4063..4082 
                     /label="Restriction Enzyme"  
     misc_feature    4867..5539 
                     /label="ColE1"  
     misc_feature    5969..6763 
                     /label="Kan/Neo"  
     misc_feature    6798..7100 
                     /label="SV40 ori"  
     misc_feature    7186..7663 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3867..3873 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3867 
                     /label="Iqsec2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACCCCCC TGGAAGAGCA GCAGGTCCAC AGCGTCTCAT ACTCTACACC 
     1081 AGTATTGCTG TCCTACTCAG GTCCTTGACT CCATGAAGCT TACCCCCTCA GGCAGGCTGG 
     1141 CAGAGAGCAG TGTGGAGGGT GATGCCCCAG GCAGTGACCT GAGCACAGCG GTTGATAGTC 
     1201 CTGGGAGCCA ACCCCCCTAC CGGCTGAGCC AACTGCCCCC AACCAGCAGC CACATGGGGG 
     1261 GCCCCCCTGC TGGCGTGGGC CTACCTTGGT CTCAGAGGGC TCGCCTCCAG CCAGCCAGTG 
     1321 TCGCCCTGAG GAAGCAGGAG GAGGAGGAGA TAAAGCGCTC CAAGGCCCTG TCAGACAGCT 
     1381 ATGAACTCTC CACAGACCTA CAGGACAAGA AGGTGGAAAT GCTGGAGAGG AAGTATGGGG 
     1441 GCTCCTTCCT GAGCCGCAGG GCTGCCAGGA CCATCCAGAC AGCCTTTCGC CAGTATCGTA 
     1501 TGAATAAGAA CTTTGAGCGA CTGCGCAGCT CGGCTTCAGA GAGCCGCATG TCCCGCCGCA 
     1561 TCATCTTGTC CAACATGCGG ATGCAGTTCT CCTTTGAGGA GTACGAGAAA GCACAGAACC 
     1621 CTGCATACTT CGAAGGCAAG CCTGCCTCAT TGGATGAGGG TGCAATGGCT GGAGCCCGGA 
     1681 GCCACCGGCT TGAACGGGGC CTCCCCTATG GAGGCTCCTG TGGTGGGGGC ATCGATGGTG 
     1741 GTGGAAGTTC AGTCACCACA TCTGGAGAGT TTTCTAATGA CATCACAGAG CTCGAGGACT 
     1801 CCTTCTCCAA ACAGGTAAAG TCTCTGGCTG AATCCATTGA TGAGGCCTTG AACTGCCACC 
     1861 CATCAGGGCC TATGTCTGAG GAGCCAGGGT CAGCCCAACT GGAGAAGCGG GAATCAAAGG 
     1921 AACAGCAAGA AGACAGCTCA GCCACATCCT TCAGTGACCT CCCCCTCTAC TTGGATGACC 
     1981 CAGTTCCTCC TCCATCACCT GAGCGACTGC CCAGCACAGA GCCCCCACCC CAAGGCCGCC 
     2041 CTGAGTTCTG GGCACCAGCT CCCCTCCCAC CAGTTCCTCC ACCAATGCCA CCAGGGACCC 
     2101 GGGAAGATGG TAGCCGTGAG GAAGGCACTC GAAGGGGTCC TGGGTGCTTG GAGTGCCGGG 
     2161 ATTTCCGGCT CCGGGCTGCA CACCTCCCCC TCCTCACCAT TGAGCCTCCC AGTGACAGTT 
     2221 CTGTAGACCT GAGTGACCGC TCAGATCGCG GCTCTGTCCA CCGCCAGCTG GTGTATGAGG 
     2281 CTGATGGCTG CAGCCCCCAT GGGACCCTGA AGCACAAGGG GCCACCAGGC AGGGCCCCAA 
     2341 TCCCACACCG CCACTACCCT GCCCCTGAGG GTCCAGCTCC AGCCCCACCA GGGCCCCTGC 
     2401 CACCAGCCCC CAATAGTGGT ACTGGGCCCA GTGGTGTGGC TGGAGGTCGG AGGTTGGGAA 
     2461 AGTGTGAGGC AGCAGGTGAG AACTCTGATG GTGGAGACAA TGAGAGCCTT GAGAGCTCCA 
     2521 GCAACTCTAA TGAGACCATC AACTGCAGCT CTGGCTCCTC CTCTCGGGAT AGCCTGAGGG 
     2581 AACCTCCAGC CACTGGCCTG TGCAAGCAGA CTTACCAGCG GGAGACAAGG CACAGCTGGG 
     2641 ACTCGCCAGC CTTCAACAAT GACGTGGTAC AAAGAAGGCA TTACAGAATT GGCCTCAACC 
     2701 TCTTCAATAA GAAGCCAGAG AAGGGTATCC AGTATTTGAT CGAGCGAGGC TTCCTGTCAG 
     2761 ACACTCCTGT GGGGGTGGCT CACTTCATCC TGGAGCGGAA AGGCCTGAGT CGGCAGATGA 
     2821 TAGGGGAATT CCTGGGGAAT CGACAGAAGC AGTTCAACAG AGATGTGTTG GACTGTGTGG 
     2881 TGGATGAGAT GGACTTCTCC TCTATGGATT TGGACGATGC ACTCAGGAAG TTCCAATCCC 
     2941 ATATCCGAGT TCAGGGTGAG GCCCAGAAAG TGGAGCGACT CATTGAAGCT TTCAGCCAGC 
     3001 GGTACTGTGT CTGTAACCCT GCCCTCGTAC GACAGTTCCG GAACCCAGAC ACTATCTTCA 
     3061 TCCTTGCTTT TGCCATCATC CTCCTCAATA CCGATATGTA CAGCCCCAGT GTCAAGGCTG 
     3121 AACGCAAGAT GAAGCTGGAT GACTTCATTA AGAACCTGAG AGGAGTTGAC AATGGTGAAG 
     3181 ACATCCCCCG AGACCTCCTA GTAGGCATCT ACCAGCGCAT CCAAGGTCGT GAACTAAGGA 
     3241 CCAACGATGA CCATGTGTCC CAGGTGCAGG CTGTGGAGCG CATGATTGTT GGCAAGAAAC 
     3301 CGGTCCTGTC GCTCCCTCAC CGTCGACTGG TTTGCTGCTG CCAGCTCTAT GAGGTGCCAG 
     3361 ATCCAAATCG CCCCCAAAGG CTGGGGCTGC ATCAGCGGGA GGTCTTCCTC TTCAATGACC 
     3421 TCCTTGTGGT CACCAAAATT TTCCAGAAGA AGAAGATCTT GGTGACATAC AGCTTCCGTC 
     3481 AGTCCTTTCC CCTGGTGGAA ATGCATATGC AGCTCTTCCA GAATTCATAT TATCAGTTTG 
     3541 GAATCAAGTT ACTATCTGCG GTACCTGGAG GGGAACGAAA AGTTCTCATT ATCTTTAATG 
     3601 CTCCTAGCCT CCAAGACAGG CTACGCTTTA CCTCTGACCT GCGGGAGTCC ATTGCTGAGG 
     3661 TGCAGGAGAT GGAGAAGTAC CGTGTGGAAT CGGAGCTGGA GAAGCAGAAA GGTATGATGC 
     3721 GGCCTAATGC CTCACAACCT GGAGGAGCCA AGGACTCAGT GAATGGGACT CTGGCCCGCA 
     3781 GTAGTCTGGA GGACACTTAT GGGGCAGGCG ATGGGCTCAA GCGGGGTGCA CTCAGTAGTT 
     3841 CCCTGCGAGA CCTCTCTGAT GCAGGGGTCT GTTATACGCG TACGCGGCCG CTCGAGCAGA 
     3901 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3961 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4021 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4081 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4141 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4201 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4261 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4321 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4381 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4441 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4501 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4561 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4621 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4681 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4741 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4801 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4861 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4921 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4981 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5041 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5101 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5161 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5221 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5281 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5341 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5401 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5461 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5521 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5581 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5641 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5701 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5761 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5821 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5881 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5941 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6001 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6061 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6121 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6181 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6241 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6301 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6361 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6421 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6481 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6541 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6601 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6661 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6721 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6781 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6841 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6901 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6961 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7021 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7081 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7141 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7201 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7261 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7321 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7381 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7441 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7501 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7561 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7621 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7681 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//