LOCUS       RR217308                7572 bp    DNA     circular  SYN 
DEFINITION  Usp33 (myc-DDK-tagged) - Rat ubiquitin specific peptidase 33 (Usp33)
ACCESSION   NM_001191094
VERSION     NM_001191094.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3729..3820 
                     /label="Myc-DDK"  
     misc_feature    3850..4435 
                     /label="PolyA signal"  
     misc_feature    3907..3926 
                     /label="Restriction Enzyme"  
     misc_feature    4711..5383 
                     /label="ColE1"  
     misc_feature    5813..6607 
                     /label="Kan/Neo"  
     misc_feature    6642..6944 
                     /label="SV40 ori"  
     misc_feature    7030..7507 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3711..3717 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3711 
                     /label="Usp33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGACTTTT CGAAATCATT GTCCACATTT GGATTCAGTT GGTGAAATAA 
     1081 CAAAAGAGGA TTTGATACAA AAATCTCATG GTGCTTGTCA GGACTGTAAA GTCAGAGGAC 
     1141 CAAACCTGTG GGCATGCCTA GAGAATAGGT GTTCCTATGT TGGCTGCGGG GAGTCACAAG 
     1201 ATCATAGCAC CATACACTCG AAGAAGACAA AGCATTATCT AACTGTGAAC CTCACCACCC 
     1261 TTCGAGTATG GTGTTATGCT TGCAGCAAAG AAGTATTCTT GGATAGAAAA TTAGGAACTC 
     1321 CTCCTTCATT ACCTCATGTA AGACAGCCTC AACAAACACA AGAAAACAGT GTCCAGGATT 
     1381 TTAAAATACC CAGTAATACA GCATTGAAAA CCCCTCTGGT TGCTGTATTT GAAGATCTGG 
     1441 ATATAGAAGT GGAAGAGGAA GATGAGCTGA AGGCTAGAGG CCTGACAGGT TTGAAAAACA 
     1501 TTGGAAATAC TTGCTATATG AATGCCGCTC TGCAAGCTCT CTCTAACTGC CCACCTTTGA 
     1561 CACAGTTCTT TCTTGATTGT GGAGGACTGG CTAGAACAGA TAAGAAACCA GCCATTTGTA 
     1621 AAAGTTATCT CAAACTAATG ACAGAATTGT GGCACAAGAG CAGGCCAGGA TCTGTTGTGC 
     1681 CTGCTAATCT GTTCCAAGGA ATTAAAACTG TAAATCCAAC TTTTCGAGGC TATTCTCAGC 
     1741 AGGATGCCCA GGAATTCCTT CGCTGTTTAA TGGATCTGCT TCATGAAGAG TTGAAGGAGC 
     1801 AGGTCATGGA AATAGAGGAA GAGCCTCAAG CACTAACTTC TGAGGAGACA GTGGAGGAAG 
     1861 AGAAGAGCCA GTCAGATGTG GATTTTCAGT CCTGCGAGTC ATGTAGCAGC AGCAGTGAGA 
     1921 AAGGGGAAAA TGAGAGTGTC TCTAAGGGCG GCCCTGAAGA CAGCACTGAG ACCACCATGC 
     1981 TCATTCAGGA TGAGGAGGAC CTAGAGATGG CCAAAGACTG GCAAAAAGAG AAGATGTGCA 
     2041 ACAAGAGCAA CAAAGCAGAT TCTGACGGAG AACCGGATAA AGACAGAGAC ACGGTGTGTG 
     2101 AAACAGTTGA CCTAAACAGC CAGGAGACCG TCAAGGTGCA GATACACGGC AGAGCCTCAG 
     2161 AATATATCAC TGATGTCCAT TTGAATGACC TATCTACACC ACAGATCCTT CCATCAAATG 
     2221 AAAGTGTTAA TCCACGTTTA TCAGCAAGTC CTCCTAAGTC AGGCAATCTG TGGCCAGGAT 
     2281 TGACACCTCC ACACAAGAAA GCTCAGTCTT CATCTCCAAA GAGGAAAAAG CAGCATAAGA 
     2341 AGTACAGAAG TGTCATCTCT GACATATTTG ATGGGACAAT CATTAGTTCA GTACAGTGTC 
     2401 TGACGTGTGA TAGGGTGTCT GTAACCCTCG AGACCTTTCA GGATCTGTCC TTGCCGATTC 
     2461 CTGGCAAGGA AGACCTGGCT AAGCTGCACT CCTCCAGTCA CCCAACTATA GTCAAAGCAG 
     2521 GGTCGTGTGG CGAAGCCTAC GCTCCACAGG GGTGGATAGC TTTCTTCATG GAGTACGTGA 
     2581 AGAGCTGGTT TTGGGGTCCA GTAGTAACCT TGCAAGATTG TCTTGCTGCC TTCTTCGCCA 
     2641 GAGATGAACT TAAAGGTGAT AATATGTACA GTTGTGAAAA ATGCAAAAAG TTGAGGAATG 
     2701 GAGTAAAGTT TTGTAAAGTA CAAAAGTTTC CTGAGATTTT GTGTATCCAC CTTAAAAGAT 
     2761 TTCGACATGA ACTGATGTTT TCCACCAAAA TTAGCACCCA TGTTTCCTTC CCCCTGGAAG 
     2821 GCCTGGACCT TCAGCCATTT CTTGCAAAGG ATAGCCCAGC TCAGATCGTG ACATATGATC 
     2881 TCCTGTCAGT CATCTGTCAT CATGGGACTG CAAGTAGTGG GCACTACATT GCCTACTGCA 
     2941 GAAACAATTT AAATAACCTG TGGTATGAGT TTGATGACCA GAGCGTCACT GAAGTTTCAG 
     3001 AGTCCACCGT ACAGAATGCC GAAGCCTACG TCCTTTTCTA CAGGAAGAGC AGTGAAGAGG 
     3061 CACAAAGGGA GAGGCGAAGG ATATCCAATT TGTTGAACAT AATGGAGCCA AGCCTCCTTC 
     3121 AGTTCTATAT ATCTCGACAG TGGTTGAATA AATTTAAGAC CTTTGCTGAA CCTGGCCCTA 
     3181 TTTCAAATAA TGACTTTCTC TGTATTCATG GAGGTATTCC TCCACGAAAA GCAAGTTATA 
     3241 TTGAAGACTT AGTTTTGATG CTGCCTCAGA ACATTTGGGA TAACCTCTAC AGCAGGTATG 
     3301 GAGGAGGGCC TGCTGTCAAC CACCTCTACA TTTGTCACAC TTGCCAAATT GAGGCAGAGA 
     3361 AGATTGAAAA ACGAAGAAAA ACTGAATTGG AAATTTTTAT TCGGCTCAAC AGAGCATTTC 
     3421 AAGAGGAAGA CTCTCCAGCT ACTTTTTACT GTATCAGCAT GCAGTGGTTT AGAGAATGGG 
     3481 AGAGTTTTGT AAAGGATCCT CCAGGTCCAA TAGACAACAC TAAAATTGCA GTCACTAAGT 
     3541 GTGGTAATGT GATGCTCAAG CAAGGAGCAG ATTCTGGTCA AATTTCAGAA GAAACATGGA 
     3601 ATTTCCTGCA ATCTATATAT GGTGGGGGAC CTGAAGTTAT CCTCCGACCT CCAGTTGTTC 
     3661 ATGTTGACCC TGATGCACTA CAAGCAGAGG AAAAGATTGA AGTAGAAACT CGCTCTTTGA 
     3721 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3781 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3841 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3901 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3961 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4021 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4081 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4141 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4201 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4261 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4321 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4381 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4441 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4501 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4561 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4621 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4681 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4741 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4801 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4861 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4921 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4981 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5041 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5101 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5161 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5221 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5281 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5341 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5401 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5461 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5521 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5581 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5641 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5701 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5761 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5821 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5881 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5941 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6001 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6061 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6121 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6181 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6241 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6301 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6361 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6421 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6481 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6541 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6601 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6661 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6721 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6781 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6841 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6901 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6961 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7021 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7081 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7141 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7201 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7261 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7321 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7381 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7441 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7501 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7561 AACGCGAATT TT
//