LOCUS       RR217307                7563 bp    DNA     circular  SYN 
DEFINITION  Actn2 (myc-DDK-tagged) - Rat actinin alpha 2 (Actn2)
ACCESSION   NM_001170325
VERSION     NM_001170325.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3720..3811 
                     /label="Myc-DDK"  
     misc_feature    3841..4426 
                     /label="PolyA signal"  
     misc_feature    3898..3917 
                     /label="Restriction Enzyme"  
     misc_feature    4702..5374 
                     /label="ColE1"  
     misc_feature    5804..6598 
                     /label="Kan/Neo"  
     misc_feature    6633..6935 
                     /label="SV40 ori"  
     misc_feature    7021..7498 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3702..3708 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3702 
                     /label="Actn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCAGATA GAGCCCGGCG TGCAGTACAA CTATGTCTAC GACGAAGATG 
     1081 AGTACATGAT CCAGGAGGAG GAGTGGGACC GCGACCTGCT CCTCGACCCG GCCTGGGAGA 
     1141 AGCAGCAGAG GAAGACTTTC ACTGCCTGGT GCAACTCGCA CCTGCGCAAA GCCGGCACCC 
     1201 AGATCGAGAA CATAGAGGAG GATTTCAGGA ATGGCCTCAA GCTCATGCTG CTTCTGGAAG 
     1261 TCATCTCAGG GGAAAGGCTT CCCAAACCTG ACCGGGGCAA AATGCGGTTC CACAAGATTG 
     1321 CGAATGTCAA CAAGGCTTTG GATTACATAG CCAGCAAGGG AGTGAAATTG GTATCTATTG 
     1381 GGGCTGAAGA AATCGTCGAT GGCAATGTGA AGATGACCCT GGGTATGATC TGGACCATCA 
     1441 TCCTCCGCTT CGCCATTCAG GACATCTCAG TGGAAGAAAC ATCTGCCAAA GAGGGCCTGC 
     1501 TGCTATGGTG TCAGAGGAAG ACAGCACCAT ACCGAAATGT GAACATCCAG AACTTCCACA 
     1561 CCAGCTGGAA AGATGGCCTT GGACTCTGTG CCCTCATCCA CAGACACCGG CCTGACCTCA 
     1621 TCGACTACTC AAAGCTTAAC AAGGATGACC CCATAGGAAA CATTAACCTG GCCATGGAAA 
     1681 TAGCAGAGAA GCACTTGGAT ATCCCTAAAA TGTTGGATGC TGAAGACATT GTCAACACTC 
     1741 CCAAACCCGA TGAAAGAGCC ATCATGACTT ACGTTTCCTG CTTCTACCAT GCTTTCGCGG 
     1801 GAGCGGAGCA GGCCGAGACG GCAGCTAACA GGATATGTAA GGTTCTTGCT GTGAATCAAG 
     1861 AGAATGAGAG GCTGATGGAA GAATATGAGA GGCTAGCGAG TGAGCTCTTG GAATGGATTC 
     1921 GACGCACAAT CCCCTGGCTG GAGAACAGGA CACCGGAGAA GACCATGCAA GCCATGCAGA 
     1981 AGAAGCTGGA GGACTTCCGG GACTATCGTC GTAAGCACAA GCCCCCAAAG GTGCAGGAGA 
     2041 AGTGCCAGCT GGAGATCAAC TTCAACACCC TGCAGACCAA GCTTCGGATC AGCAACCGGC 
     2101 CTGCCTTCAT GCCCTCCGAG GGCAAGATGG TGTCGGACAT TGCTGGTGCC TGGCAGAGGT 
     2161 TGGAGCAGGC CGAGAAGGGC TATGAGGAAT GGCTATTGAA TGAGATTCGA AGACTGGAGC 
     2221 GCTTGGAGCA CTTGGCTGAG AAGTTCAGGC AGAAGGCCTC CACCCATGAG ACATGGGCTT 
     2281 ACGGCAAAGA ACAGATCTTG CTGCAGAAGG ACTATGAGTC TGCGTCCCTG ACGGAAGTTC 
     2341 GAGCCCTACT TCGGAAGCAC GAGGCCTTTG AGAGTGACCT GGCGGCACAC CAGGATCGTG 
     2401 TGGAACAGAT TGCAGCCATT GCTCAGGAGC TCAATGAACT GGACTACCAC GATGCTGTGA 
     2461 ACGTCAATGA TCGTTGCCAG AAAATTTGCG ACCAGTGGGA TAGATTGGGG ACGCTCACCC 
     2521 AGAAGAGGAG GGAGGCCTTG GAGAGAACCG AGAAGTTGCT AGAAACAATT GACCAGCTGC 
     2581 ACCTGGAGTT TGCCAAGAGA GCTGCGCCCT TCAACAACTG GATGGAGGGC GCCATGGAGG 
     2641 ACCTGCAGGA CATGTTCATC GTCCACAGCA TTGAGGAGAT CCAGAGTCTG ATCACCGCAC 
     2701 ATGAGCAGTT CAAGGCCACG CTGCCCGAGG CCGATGGGGA GCGGCAGTCC ATCCTAGCCA 
     2761 TCCAGAATGA GGTGGAGAAG GTGATCCAGA GCTACAGTAT CCGGATCAGC TCCAGCAACC 
     2821 CCTACAGCAC TGTCACCATG GACGAGCTGC GCAACAAGTG GGACAAGGTG AAGCAGCTGG 
     2881 TGCCAGTCCG GGATCAGTCC CTGCAGGAGG AGCTGGCACG CCAACATGCT AATGAGCGCC 
     2941 TACGGCGCCA GTTTGCTGCC CAGGCCAATG CCATCGGCCC CTGGATCCAG AACAAGATGG 
     3001 AGGAGATCGC TCGGAGCTCC ATCCAGATCA CAGGGGCCCT GGAGGACCAG ATGAACCAGC 
     3061 TGAAGCAGTA CGAGCACAAC ATCATCAACT ACAAGAACAA CATTGACAAG CTGGAGGGCG 
     3121 ACCACCAGCT CATCCAGGAA GCCCTGGTCT TCGACAACAA GCACACCAAC TACACCATGG 
     3181 AGCATATCCG TGTCGGATGG GAGTTGCTGC TCACAACTAT CGCCAGAACC ATCAATGAGG 
     3241 TGGAGACCCA GATCCTGACA AGAGATGCCA AGGGCATCAC CCAGGAGCAG ATGAATGAGT 
     3301 TCAGAGCCTC CTTCAACCAC TTTGACAGGA GGAAGAATGG CCTGATGGAT CATGAGGATT 
     3361 TCAGAGCCTG CCTCATTTCC ATGGGCTATG ACTTGGGTGA AGCTGAGTTT GCCCGAATTA 
     3421 TGACTCTGGT TGACCCCAAC GGACAAGGCA CGGTCACCTT CCAGTCCTTC ATTGACTTCA 
     3481 TGACTAGAGA GACCGCAGAC ACGGACACTG CAGAACAGGT CATCGCCTCC TTCCGGATTC 
     3541 TGGCTTCTGA TAAGCCTTAC ATCTTGGCAG AGGAACTTCG TCGAGAGCTG CCTCCGGATC 
     3601 AGGCCCAGTA CTGCATCAAG AGAATGCCCC CATACTCAGG CCCAGGCAGT GTCCCCGGGG 
     3661 CCCTGGACTA CACTGCCTTC TCCTCTGCCC TCTACGGGGA GAGCGACCTC ACGCGTACGC 
     3721 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3781 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3841 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3901 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3961 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4021 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4081 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4141 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4201 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4261 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4321 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4381 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4441 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4501 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4561 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4621 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4681 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4741 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4801 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4861 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4921 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4981 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5041 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5101 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5161 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5221 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5281 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5341 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5401 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5461 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5521 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5581 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5641 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5701 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5761 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5821 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5881 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5941 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6001 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6061 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6121 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6181 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6241 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6301 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6361 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6421 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6481 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6541 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6601 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6661 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6721 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6781 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6841 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6901 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6961 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7021 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7081 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7141 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7201 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7261 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7321 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7381 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7441 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7501 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7561 TTT
//