LOCUS       RR217305                7569 bp    DNA     circular  SYN 
DEFINITION  Epha5 (myc-DDK-tagged) - Rat EPH receptor A5 (Epha5), transcript variant 2
ACCESSION   NM_024367
VERSION     NM_024367.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3724..3815 
                     /label="Myc-DDK"  
     misc_feature    3845..4430 
                     /label="PolyA signal"  
     misc_feature    3902..3921 
                     /label="Restriction Enzyme"  
     misc_feature    4706..5378 
                     /label="ColE1"  
     misc_feature    5808..6602 
                     /label="Kan/Neo"  
     misc_feature    6637..6939 
                     /label="SV40 ori"  
     misc_feature    7025..7502 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3699..3706 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3699 
                     /label="Epha5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGGCTCC GGGCCCCGCG GTGCGGGACG CCGACGGACC CAGGGCAGAG 
     1081 GTGGCGGCGG CGACACCCCC CGCGTCCCTG CCTCTCTGGC AGGCTGCTAT TCCGCACCTC 
     1141 TAAAGGGGCC CCTCTGGACG TGCCTTCTCC TGTGTGCGGC GCTCCGGACC CTTTTGGCCA 
     1201 GCCCCAGTAA CGAAGTGAAT TTATTGGATT CGCGCACTGT CCTGGGAGAC CTTGGATGGA 
     1261 TTGCTTTTCC AAAGAATGGG TGGGAAGAGA TTGGTGAAGT TGATGAAAAC TATGCCCCCA 
     1321 TCCACACCTA TCAAGTGTGC AAAGTTATGG AACAGAATCA GAATAATTGG CTGTTGACCA 
     1381 GTTGGATCTC TAACGAAGGT GCTTCCAGAA TTTTTATTGA ACTCAAGTTT ACTCTGAGGG 
     1441 ATTGCAACAG CCTTCCTGGA GGACTGGGGA CTTGCAAGGA GACCTTTAAC ATGTATTATT 
     1501 TTGAGTCGGA TGATGAGAAT GGGAGAAATA TCAAAGAGAA CCAGTACATC AAGATCGATA 
     1561 CCATTGCTGC TGATGAGAGC TTCACCGAAC TTGACCTTGG AGACCGGGTC ATGAAGCTGA 
     1621 ATACGGAGGT CAGAGATGTA GGACCTCTGA GCAAAAAGGG ATTTTATCTT GCTTTCCAAG 
     1681 ATGTCGGTGC TTGCATCGCT CTGGTTTCTG TCCGTGTCTA CTATAAAAAA TGTCCTTCTG 
     1741 TAGTTAGACA TTTGGCTGTT TTCCCTGACA CGATCACTGG AGCAGATTCT TCCCAGTTGC 
     1801 TAGAGGTGTC AGGCTCCTGC GTCAACCATT CTGTGACAGA CGATCCTCCC AAAATGCATT 
     1861 GCAGTGCTGA AGGGGAGTGG CTGGTTCCCA TCGGGAAATG CATGTGCAAG GCCGGATATG 
     1921 AAGAGAAAAA TGGTACCTGT CAAGTGTGCA GACCTGGGTT CTTCAAAGCC TCTCCTCACA 
     1981 GCCAGACCTG CAGCAAATGT CCACCTCACA GTTACACCCA TGAGGAAGCT TCCACCTCTT 
     2041 GTGTCTGTGA AAAGGATTAT TTCAGGAGGG AATCTGATCC GCCCACAATG GCATGCACAA 
     2101 CTCCTTCTCC AGTCACCAAC GTGAAGAAGG GGAAAATCGC AAAGAACAGC ATTTCTTTGT 
     2161 CTTGGCAAGA GCCAGATCGC CCCAATGGGA TCATCCTGGA GTACGAAATC AAGTACTTTG 
     2221 AAAAGGACCA AGAGACCAGC TACACAATTA TCAAGTCTAA AGAGACCACT ATTACGGCAG 
     2281 AGGGCCTGAA ACCTGCGTCT GTGTATGTCT TCCAAATTCG AGCACGTACA GCAGCAGGCT 
     2341 ACGGCGTCTT CAGTCGACGG TTTGAGTTTG AAACCACACC AGTGTTTGCA GCATCTAATG 
     2401 ATCAAAGCCA GATTCCCATT ATTGCGGTGT CAGTGACGGT GGGAGTCATC TTGTTGGCAG 
     2461 TGATGATCGG CTTCCTCCTC AGTGGCAGTT GCTGCGAATG TGGCTGTGGG AGGGCTTCTT 
     2521 CCCTGTGCGC TGTTGCCCAT CCAAGCCTAA TATGGCGGTG TGGCTACAGC AAAGCAAAGC 
     2581 AGGATCCAGA AGAGGAAAAG ATGCACTTTC ATAACGGGCA CATTAAACTG CCAGGAGTAA 
     2641 GAACGTACAT TGATCCACAC ACCTACGAAG ATCCCACTCA AGCCGTTCAT GAATTTGCCA 
     2701 AGGAGATCGA GGCCTCATGC ATCACCATTG AGAGAGTTAT TGGAGCAGGT GAATTTGGTG 
     2761 AAGTTTGTAG TGGACGTTTG AAACTACCCG GAAAAAGAGA ATTGCCTGTG GCTATCAAAA 
     2821 CTCTTAAAGT AGGCTACACT GAAAAGCAGC GCAGAGATTT TCTGAGTGAA GCGAGTATCA 
     2881 TGGGGCAGTT TGATCATCCA AACATCATCC ATCTAGAAGG CGTTGTGACT AAAAGTAAAC 
     2941 CTGTGATGAT AGTGACAGAG TACATGGAGA ATGGCTCCTT AGACACATTT TTAAAGAAAA 
     3001 ACGATGGCCA GTTCACTGTG ATTCAGCTTG TTGGCATGCT GAGAGGCATC GCTGCAGGAA 
     3061 TGAAGTACCT TTCTGACATG GGCTACGTGC ACAGAGACCT TGCTGCTAGA AACATCTTAA 
     3121 TCAACAGTAA CCTTGTGTGC AAAGTGTCTG ACTTTGGACT TTCCAGGGTG CTGGAAGATG 
     3181 ATCCTGAGGC AGCCTATACC ACAAGGGGAG GCAAAATTCC AATCAGGTGG ACTGCTCCAG 
     3241 AAGCAATAGC TTTTCGAAAG TTTACCTCTG CCAGTGATGT CTGGAGCTAT GGAATCGTAA 
     3301 TGTGGGAAGT TGTGTCCTAT GGAGAGAGAC CGTACTGGGA GATGACCAAT CAGGATGTGA 
     3361 TCAAGGCAGT GGAAGAAGGC TACCGCCTGC CAAGCCCCAT GGATTGTCCT GCTGCCCTCT 
     3421 ATCAATTAAT GCTGGATTGC TGGCAGAAAG ATCGCAACAG CAGGCCCAAG TTTGATGACA 
     3481 TAGTCAACAT GCTGGATAAG CTGATACGAA ACCCAAGTAG TTTGAAGACA CTGGTGAACG 
     3541 CGTCAAGCAG GGTATCTACT TTGTTGGCAG AACATGGATC TTTGGGGTCT GGGGCCTACA 
     3601 GATCAGTAGG TGAATGGCTA GAAGCAATCA AAATGGGCCG GTACACAGAG ATTTTCATGG 
     3661 AAAATGGATA CAGTTCAATG GACGCTGTGG CTCAGGTGAC CTTGGAGAGC GGACCGACGC 
     3721 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3781 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3841 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3901 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3961 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4021 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4081 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4141 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4201 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4261 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4321 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4381 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4441 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4501 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4561 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4621 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4681 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4741 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4801 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4861 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4921 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4981 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5041 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5101 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5161 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5221 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5281 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5341 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5401 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5461 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5521 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5581 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5641 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5701 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5761 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5821 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5881 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5941 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6001 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6061 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6121 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6181 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6241 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6301 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6361 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6421 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6481 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6541 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6601 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6661 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6721 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6781 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6841 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6901 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6961 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7021 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7081 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7141 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7201 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7261 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7321 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7381 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7441 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7501 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7561 GCGAATTTT
//