LOCUS       RR217301                7545 bp    DNA     circular  SYN 
DEFINITION  Rad54b (myc-DDK-tagged) - Rat RAD54 homolog B (S. cerevisiae) (Rad54b)
ACCESSION   NM_001191755
VERSION     NM_001191755.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3702..3793 
                     /label="Myc-DDK"  
     misc_feature    3823..4408 
                     /label="PolyA signal"  
     misc_feature    3880..3899 
                     /label="Restriction Enzyme"  
     misc_feature    4684..5356 
                     /label="ColE1"  
     misc_feature    5786..6580 
                     /label="Kan/Neo"  
     misc_feature    6615..6917 
                     /label="SV40 ori"  
     misc_feature    7003..7480 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3684..3690 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3684 
                     /label="Rad54b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGACGTTCT GCAGCACCAA GCCAGGTGCG GGGAAATTCC TCTAAAAAAG 
     1081 CAAGATTTGT ACCTCCAGGA AGAAGTACTA CAGGTGTGAG TAAGGAGATT TCGATGAGTC 
     1141 CAGAGGCAAA ACTGGTTCAG GGCGCCCACC AGTCACAAGG GGATGCAGGT GTGTGCAGTT 
     1201 CCAATCCTTG GCTCACTGAG GGAAGCCCTG TGGAGATGGG TGACGGGGCT AGAGTAGATC 
     1261 CGCTTTCTCC AGTTCACTTG GCCTCTAAAG AAATCATAGA GTCCAAGGCA CAAGAAGAGG 
     1321 AAGCTAGCAG CTTGCTTAAA TATTTCAGTG TCGTTTGGTG TAAAGCTTCA AAGAAAAAAC 
     1381 ACAAGAAATG GGAAGGCGAT GCTATTCTTA TTGTAAAAGG AAGGTCGTTC ATATTGAAAG 
     1441 ATATGGAAGG AAAAGATATT GGAAGAGGCA TGGGATATAA ATTCAAAGAG CTTGAAAATG 
     1501 TAGAAGAAGG CCAAACATTG ATGCTTGGTG GGAAAGAAAT AGAGATCCTG GGTACAGTCT 
     1561 CTGCTGAGGA CTTCAATAGT GGCAAGTGTT TTCAGGCTGG AAGCGGAAGT CCTGCCGTCC 
     1621 CAAGTTCTCA GGCTGCCAGG AAATGCTTTT CTAACCCTTT CAAGAGTGTG TGTCAGTCAA 
     1681 CTCAAGCAAG GAGAGACAGA GGGAATGACT GGCAAAATTG CAAACCTCGC TATGACCCAT 
     1741 ACACACCAAA TGCCCTTGTT ATGCCTCGAC CAGACAAGAG TCACCAGTGG GCGTTTAATA 
     1801 GGAGCTGCCT TCCTACTGTG GATGTGGTGA TAGACCCTCA CCTTGTACGT CACCTCCGAC 
     1861 CACACCAGAA AGATGGGGTC GCCTTCCTCT ATGAGTGTGT GATGGGAATG AGAGCAGTTG 
     1921 GCAAATGTGG TGCCATTCTT GCTGATGAGA TGGGATTAGG GAAAACCCTG CAGTGTATCT 
     1981 CGCTCATCTG GACCCTCCAG TGTCAGGGGC CTTACGGGGG CAAGCCAATA GTAAAGAGGA 
     2041 CACTTATTGT TACCCCTGGA AGCTTGGTGA ATAATTGGAG AAAAGAATTT CAAAAATGGC 
     2101 TGGGAAGCGA AAGGATCAAG ATATTCACTG TTGATCAGGA CCATAAAGTC GAGGAGTTTA 
     2161 TCAATTCTGC ATTTCACTCT GTTCTTATCA TCAGTTATGA AATGTTACTT CGTTCTCTAG 
     2221 ATCGAATTAA GACCATAACA TTTGGCCTTC TGATTTGTGA TGAGGGGCAC CGGTTGAAGA 
     2281 ACAGTGGTAT TAAGACAACA GCAGCCCTTT CTAGCCTCTC TTGTGAGAAG AGAGTAATTC 
     2341 TGACTGGTAC CCCAGTTCAG AATGATTTGC AAGAGTTTTT TGCATTAGTT GATTTTGTTA 
     2401 ATCCAGGAAT CTTAGGGTCT CTGTCATCTT ATAGGAGGAT ATATGAAGAG CCCATTGTTA 
     2461 TGTCAAGAGA GCCTTCTGCT TCCAAGGAAG AGAGAGAGTT AGGGGAGAGA AGAACAACTG 
     2521 AGCTCACACG CCTCACTGGA TGTTTCATCC TTAGAAGAAC TCAGGAGGTC ATCAATAAAT 
     2581 ATCTCCCCCC TAAGATAGAG AACGTAGTCT TTTGCCGACC AGGAGCACTG CAGATTGAGC 
     2641 TTTACCGAAA GCTGCTGAGT TCTCAATCTG TCAGGTTCTG TCTGCAAGGC CTTTTGGGAA 
     2701 ATAGTGCTCA CCTCATTTGC ATAGGCGCCC TGAAGAAGCT ATGCAATCAT CCTCGCCTCT 
     2761 TGTTCAGCTT TGTGAAGGGT AAAGAATTTA ACTCGAGTCG TGATGAAAAT GAAGAAAGGA 
     2821 GCCTTTGCCA AGGCTTGCTG ACTGTGTTCC CTGCTGGCTA CAACCTGCTG CAGCTCTCCG 
     2881 AGAGCGAGTC AGGGAAACTA CAGGTGTTAG TAAAGCTCCT GGCGGCGATC TCTGAACTTC 
     2941 GTCCCACTGA AAAAGTGATT TTGGTATCCA ACTACAGACA AACCTTGAAT CTTTTAGAAG 
     3001 AAGTATGCAA GTGTCATGGA TATGCTTGTG CAAGACTTGA TGGACAAACA CCAGTCTCTC 
     3061 AAAGACAGCA AATTGTTGAT AATTTCAATA GTAAATACTC TACTGATTTT ATTTTTTTAT 
     3121 TAAGTTCAAA AGCTGGTGGT GTGGGACTTA ATCTTATTGG AGGATCTCAC TTAATTCTTT 
     3181 ATGATATTGA TTGGAACCCA GCTACTGACA TCCAGGCCAT GGCTAGAGTG TGGAGAGACG 
     3241 GCCAGAAACA CCCTGTCCAT GTTTACAGAC TCCTGACTAC AGGTACAATA GAGGAAAAGA 
     3301 TCTATCAAAG GCAGATCAGT AAGCAAGGCC TTTCTGGGGC GGTTGTAGAC CTAACCAGAT 
     3361 CATCCGAACA GATCCAGTTT TCAGTGGAAG AGCTTAAAAA CTTGTTCACA TTACATGAAA 
     3421 ATTCACATTG TGTTACTCAT GACCTGCTTG ACTGTGAGTG CACAGGAGAG AAAGATCACT 
     3481 CTGATGATGC GTCGGAAAAC CCTGTCACCT CTAGACAGTG TCAGCTTGGC CCTCAGAAGT 
     3541 CCAGCTCCTC GAGACCTCTG TCCATGTCAC AGCTGAAGCA GTGGAAACAT TTTTCAGGAG 
     3601 ACCATTTAAA TCTCCCAGAC CCATTTCTCG AAAGAATAAG GGAAAATGTG TCCTTCTTTT 
     3661 TCCAGAATAT AACCAACCAA GCTCCTGCCA TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3721 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3781 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3841 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3901 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3961 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4021 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4081 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4141 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4201 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4261 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4321 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4381 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4441 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4501 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4561 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4621 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4681 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4741 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4801 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4861 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4921 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4981 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5041 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5101 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5161 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5221 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5281 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5341 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5401 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5461 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5521 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5581 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5641 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5701 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5761 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5821 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5881 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5941 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6001 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6061 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6121 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6181 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6241 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6301 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6361 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6421 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6481 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6541 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6601 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6661 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6721 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6781 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6841 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6901 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6961 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7021 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7081 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7141 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7201 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7261 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7321 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7381 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7441 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7501 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//