LOCUS       RR217280                7485 bp    DNA     circular  SYN 
DEFINITION  Ube3a (myc-DDK-tagged) - Rat ubiquitin protein ligase E3A (Ube3a)
ACCESSION   NM_001191837
VERSION     NM_001191837.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3642..3733 
                     /label="Myc-DDK"  
     misc_feature    3763..4348 
                     /label="PolyA signal"  
     misc_feature    3820..3839 
                     /label="Restriction Enzyme"  
     misc_feature    4624..5296 
                     /label="ColE1"  
     misc_feature    5726..6520 
                     /label="Kan/Neo"  
     misc_feature    6555..6857 
                     /label="SV40 ori"  
     misc_feature    6943..7420 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3624..3630 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3624 
                     /label="Ube3a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACAGCT TGTAAAAGAT CATCAGGAGA ATCCCAGTCT GAGGACATTG 
     1081 AAGCTAGCCG AATGAAGCGA GCAGCTGCAA AGCATCTAAT AGAGCGCTAC TACCATCAGT 
     1141 TAACTGAGGG CTGTGGAAAT GAGGCCTGCA CGAATGAGTT CTGTGCTTCC TGTCCAACTT 
     1201 TTCTTCGTAT GGATAACAAT GCAGCAGCTA TTAAAGCTCT TGAGCTTTAT AAAATTAATG 
     1261 CAAAACTCTG TGATCCTCAT CCCTCCAAGA AAGGAGCAAG CTCAGCTTAC CTTGAGAACT 
     1321 CAAAAGGTGC ATCTAACAAC TCAGAGATAA AAATGAACAA GAAGGAAGAG TTTAAAGAGG 
     1381 TGGACTACCT AACTGAAGAG AAAGTATATG AAATTTATGA ATTTTGTAGG GAGAGAGAGG 
     1441 ATTACTCCCC TTTAATTCGT GTAATTGGAA GAATATTTTC TAGTGCTGAG GCACTGGTTC 
     1501 AGAGCTTTCG GAAAGTCAAA CAGCACACTA AGGAGGAATT GAAATCTCTT CAAGAGAAGG 
     1561 ATGAAGACAA GGATGAAGAT GAAAAGGAAA AAGCTGCGTG TTCTGCTGCT GCTATGGAAG 
     1621 AAGACTCAGA AGCATCTTCT TCAAGGATGG GTGATAGCTC ACAGGGAGAC AACAATGTAC 
     1681 AAAAATTAGG TCCTGATGAT GTGACTGTGG ATATTGATGC TATAAGAAGG GTCTACAGCA 
     1741 GTTTGCTCTC TGATGAAAAA ATAGAAACTG CCTTCCTGAA TGCACTTGTA TATCTGTCAC 
     1801 CTAACGTGGA ATGTGATTTG ACATATCATA ATGTGTACAC TCGAGACCCT AATTATCTCA 
     1861 ATTTGTTCAT TATTGTAATG GAAAATAGGA ATCTCCACAG TCCTGAATAT CTGGAAATGG 
     1921 CATTGCCGTT ATTTTGCAAA GCTATGAGTA AGCTACCCCT TGCAGCTCAA GGAAAACTGA 
     1981 TTAGGCTGTG GTCTAAATAC AGTGCTGACC AGATTCGGAG AATGATGGAA ACATTTCAGC 
     2041 AACTTATTAC CTACAAAGTC ATAAGCAATG AATTTAATAG CCGAAATCTA GTGAATGATG 
     2101 ATGATGCCAT TGTTGCTGCT TCAAAGTGTT TGAAAATGGT TTACTATGCA AATGTAGTGG 
     2161 GAGGGGACGT GGACACAAAT CATAATGAGG AAGATGATGA AGAGCCTATA CCTGAGTCCA 
     2221 GTGAATTGAC ACTTCAGGAG CTTCTGGGAG AGGAAAGAAG AAATAAGAAA GGTCCTCGAG 
     2281 TGGATCCACT AGAAACCGAA ATTGGCGTTA AAACTCTAGA TTGTCGAAAA CCACTTATCT 
     2341 CCTTTGAAGA ATTCATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAC AAAGATTATA 
     2401 CTTTTTTCAA AGTTGAAACA GAGAACAAAT TCTCTTTTAT GACATGTCCC TTTATATTGA 
     2461 ATGCTGTCAC AAAGAATCTG GGATTGTATT ATGACAATAG AATTCGCATG TACAGTGAAA 
     2521 GAAGAATCAC TGTTCTTTAC AGCCTAGTTC AAGGACAGCA GTTGAATCCA TATTTGAGAC 
     2581 TTAAAGTCAG ACGTGACCAT ATTATAGATG ATGCACTTGT CCGGCTAGAG ATGATCGCTA 
     2641 TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA GAACAAGGAG 
     2701 TAGATGAGGG AGGCGTTTCC AAAGAGTTTT TTCAGTTGGT TGTGGAGGAA ATCTTTAATC 
     2761 CAGATATTGG TATGTTCACA TACGATGAAG CTACAAGATT ATTTTGGTTT AATCCATCTT 
     2821 CTTTTGAAAC TGAGGGTCAA TTTACTCTGA TTGGCATAGT CCTGGGTCTG GCTATTTACA 
     2881 ATAACTGTAT ACTGGATGTC CATTTTCCCA TGGTTGTGTA CAGGAAGCTA ATGGGGAAAA 
     2941 AAGGAACGTT TTGTGACTTG GGAGACTCTC ACCCAATCTT ATATCAGAGT TTAAAGGATT 
     3001 TATTGGAATA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG ATATCACAGA 
     3061 CAGATCTTTT TGGTAACCCA ATGATGTATG ATCTAAAAGA AAATGGTGAT AAAATTCCAA 
     3121 TTACAAATGA AAACAGGAAG GAATTTGTCA GTCTCTATTC AGACTACATT CTCAATAAAT 
     3181 CTGTAGAAAA ACAATTCAAG GCATTTCGCA GAGGTTTTCA TATGGTGACT AATGAATCAC 
     3241 CCTTAAAATA CTTATTCAGA CCAGAAGAAA TTGAATTACT TATATGTGGA AGCCGGAATC 
     3301 TCGATTTCCA GGCACTAGAA GAAACTACAG AGTATGATGG TGGCTATACG AGAGAATCTG 
     3361 TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCGTTTAC AGATGAACAG AAAAGACTTT 
     3421 TCCTGCAGTT TACAACAGGC ACAGACAGAG CACCTGTTGG AGGACTCGGA AAATTGAAGA 
     3481 TGATTATAGC CAAAAATGGC CCAGACACAG AAAGGTTACC TACATCTCAT ACTTGCTTTA 
     3541 ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA TTGCTGAAGG 
     3601 CCATCACATA TGCCAAAGGA TTTGGCATGC TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3661 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3721 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3781 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3841 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3901 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3961 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4021 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4081 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4141 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4201 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4261 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4321 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4381 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4441 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4501 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4561 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4621 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4681 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4741 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4801 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4861 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4921 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4981 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5041 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5101 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5161 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5221 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5281 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5341 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5401 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5461 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5521 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5581 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5641 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5701 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5761 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5821 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5881 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5941 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6001 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6061 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6121 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6181 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6241 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6301 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6361 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6421 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6481 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6541 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6601 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6661 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6721 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6781 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6841 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6901 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6961 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7021 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7081 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7141 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7201 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7261 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7321 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7381 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7441 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//